(Circular) (Six-base) MAPSORT of: pFN21AB9842.seq Check: 5925 from: 1 to: 5785 pFN21AB9842 5785 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3595 278 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2259 2565 3128 1937 Size: 322 306 563 4594 Fragments arranged by size: 4594 563 322 306 Acc65I G'GTAC_C Cuts at: 3106 3106 Size: 5785 AclI AA'CG_TT Cuts at: 1563 3912 4285 1563 Size: 2349 373 3063 Fragments arranged by size: 3063 2349 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 3045 3848 5232 5500 1632 Size: 767 646 803 1384 268 1917 Fragments arranged by size: 1917 1384 803 767 646 268 AfeI AGC'GCT Cuts at: 2480 2480 Size: 5785 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5588 AflIII A'CryG_T Cuts at: 1203 4684 5661 1203 Size: 3481 977 1327 Fragments arranged by size: 3481 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 4514 4514 Size: 5785 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AlwNI CAG_nnn'CTG Cuts at: 2511 3528 5100 2511 Size: 1017 1572 3196 Fragments arranged by size: 3196 1572 1017 ApaLI G'TGCA_C Cuts at: 3842 4998 3842 Size: 1156 4629 ApoI r'AATT_y Cuts at: 1493 2492 3094 3320 3472 5581 1493 Size: 999 602 226 152 2109 1697 Fragments arranged by size: 2109 1697 999 602 226 152 AseI AT'TA_AT Cuts at: 160 1032 4339 160 Size: 872 3307 1606 Fragments arranged by size: 3307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 3110 1870 Size: 73 1167 4545 Fragments arranged by size: 4545 1167 73 BamHI G'GATC_C Cuts at: 2640 3115 3482 2640 Size: 475 367 4943 Fragments arranged by size: 4943 475 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3106 4561 618 Size: 325 217 627 1319 1455 1842 Fragments arranged by size: 1842 1455 1319 627 325 217 BanII G_rGCy'C Cuts at: 1391 2890 3104 1391 Size: 1499 214 4072 Fragments arranged by size: 4072 1499 214 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4814 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2860 2894 3976 4010 1903 Size: 34 923 34 1082 34 3678 Fragments arranged by size: 3678 1082 923 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3677 4887 3677 Size: 1210 4575 BclI T'GATC_A Cuts at: 1548 2679 3006 1548 Size: 1131 327 4327 Fragments arranged by size: 4327 1131 327 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4396 136 Size: 107 122 71 631 605 2724 1525 Fragments arranged by size: 2724 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2059 5780 2059 Size: 3721 2064 BlpI GC'TnA_GC Cuts at: 2186 3202 2186 Size: 1016 4769 Bme1580I G_kGCm'C Cuts at: 1790 3069 3846 5002 1790 Size: 1279 777 1156 2573 Fragments arranged by size: 2573 1279 1156 777 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5785 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2644 3516 4474 Size: 416 53 83 399 24 1227 872 958 Cuts at: 4474 442 Size: 1753 Fragments arranged by size: 1753 1227 958 872 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4445 1239 Size: 3206 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2966 3910 4775 5073 2966 Size: 944 865 298 3678 Fragments arranged by size: 3678 944 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2374 2849 4448 882 Size: 617 875 475 1599 2219 Fragments arranged by size: 2219 1599 875 617 475 BsaAI yAC'GTr Cuts at: 493 1204 3576 493 Size: 711 2372 2702 Fragments arranged by size: 2702 2372 711 BsaBI GATnn'nnATC Cuts at: 5779 5779 Size: 5785 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3592 3974 4609 275 Size: 53 83 186 936 2059 382 635 1451 Fragments arranged by size: 2059 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4218 4616 4890 5037 5448 4218 Size: 398 274 147 411 4555 Fragments arranged by size: 4555 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5560 Fragments arranged by size: 5560 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2969 1186 Size: 1783 4002 BseYI C'CCAG_C Cuts at: 2194 2584 4988 5484 2194 Size: 390 2404 496 2495 Fragments arranged by size: 2495 2404 496 390 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2242 2884 3235 3996 5024 1540 Size: 275 186 241 642 351 761 1028 2301 Fragments arranged by size: 2301 1028 761 642 351 275 241 186 BsiHKAI G_wGCw'C Cuts at: 3846 3931 5002 3846 Size: 85 1071 4629 Fragments arranged by size: 4629 1071 85 BsiWI C'GTAC_G Cuts at: 2711 2711 Size: 5785 BsmI GAATG_Cn' Cuts at: 3296 3389 3296 Size: 93 5692 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2890 3069 3104 3846 3931 5002 1391 Size: 399 1100 179 35 742 85 1071 2174 Fragments arranged by size: 2174 1100 1071 742 399 179 85 35 BspEI T'CCGG_A Cuts at: 5448 5448 Size: 5785 BspHI T'CATG_A Cuts at: 2832 3674 5404 2832 Size: 842 1730 3213 Fragments arranged by size: 3213 1730 842 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2271 3140 844 Size: 1427 869 3489 Fragments arranged by size: 3489 1427 869 BsrBI CCG'CTC Cuts at: 1394 1823 2244 3672 1394 Size: 429 421 1428 3507 Fragments arranged by size: 3507 1428 429 421 BsrDI GCAATG_nn' Cuts at: 65 1248 4280 4454 65 Size: 1183 3032 174 1396 Fragments arranged by size: 3032 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4429 4616 1700 Size: 110 2619 187 2869 Fragments arranged by size: 2869 2619 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 2207 3845 4857 2207 Size: 1638 1012 3135 Fragments arranged by size: 3135 1638 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5785 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5785 BstXI CCAn_nnnn'nTGG Cuts at: 2657 2657 Size: 5785 BstYI r'GATC_y Cuts at: 1972 2059 2640 3115 3482 3867 3884 5336 5422 Size: 87 581 475 367 385 17 1452 86 Cuts at: 5422 5434 5780 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 581 475 385 367 346 87 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2971 513 Size: 552 939 967 3327 Fragments arranged by size: 3327 967 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5046 Fragments arranged by size: 5046 683 56 BtsI GCAGTG_nn' Cuts at: 2672 3301 4095 4115 2672 Size: 629 794 20 4342 Fragments arranged by size: 4342 794 629 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 3089 3089 Size: 5785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4792 809 Size: 507 3476 1802 Fragments arranged by size: 3476 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2239 2280 2442 Size: 54 1425 50 275 18 409 41 162 Cuts at: 2442 2956 3232 4121 5750 8 Size: 514 276 889 1629 43 Fragments arranged by size: 1629 1425 889 514 409 276 275 162 54 50 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 2239 3232 1537 Size: 275 427 993 4090 Fragments arranged by size: 4090 993 427 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3061 3715 1353 Size: 1708 654 3423 Fragments arranged by size: 3423 1708 654 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2641 4372 4746 4892 387 Size: 1064 138 1052 1731 374 146 1280 Fragments arranged by size: 1731 1280 1064 1052 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2399 3045 3848 4445 5232 5500 1239 Size: 393 767 646 803 597 787 268 1524 Fragments arranged by size: 1524 803 787 767 646 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2360 2360 Size: 5785 EcoO109I rG'GnC_Cy Cuts at: 2838 2838 Size: 5785 EcoRI G'AATT_C Cuts at: 3094 3472 3094 Size: 378 5407 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2500 2500 Size: 5785 FspI TGC'GCA Cuts at: 4291 4291 Size: 5785 HaeII r_GCGC'y Cuts at: 1678 2482 2869 4932 1678 Size: 804 387 2063 2531 Fragments arranged by size: 2531 2063 804 387 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4426 4458 4500 4532 1373 Size: 32 135 32 2854 32 42 32 2626 Fragments arranged by size: 2854 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2389 3129 3375 669 Size: 988 281 451 740 246 3079 Fragments arranged by size: 3079 988 740 451 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5785 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KpnI G_GTAC'C Cuts at: 3110 3110 Size: 5785 MfeI C'AATT_G Cuts at: 3384 3384 Size: 5785 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4899 5083 1305 Size: 3594 184 2007 Fragments arranged by size: 3594 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2444 2958 10 Size: 54 1425 343 612 514 2837 Fragments arranged by size: 2837 1425 612 514 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2356 2426 2831 3743 4102 4261 518 Size: 648 1190 70 405 912 359 159 2042 Fragments arranged by size: 2042 1190 912 648 405 359 159 70 MspA1I CmG'CkG Cuts at: 1606 2925 3201 3878 5026 5271 1606 Size: 1319 276 677 1148 245 2120 Fragments arranged by size: 2120 1319 1148 677 276 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2971 513 Size: 552 939 967 3327 Fragments arranged by size: 3327 967 939 552 NdeI CA'TA_TG Cuts at: 387 2498 387 Size: 2111 3674 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 3143 4688 1132 Size: 97 1914 1545 2229 Fragments arranged by size: 2229 1914 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5785 PciI A'CATG_T Cuts at: 4684 4684 Size: 5785 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5785 PfoI T'CCnGG_A Cuts at: 2850 2850 Size: 5785 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2838 2838 Size: 5785 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5785 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PstI C_TGCA'G Cuts at: 830 3137 830 Size: 2307 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 PvuII CAG'CTG Cuts at: 2925 2925 Size: 5785 SalI G'TCGA_C Cuts at: 1936 3127 1936 Size: 1191 4594 SapI GCTCTTCn'nnn_ Cuts at: 3061 3061 Size: 5785 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 4033 5745 4033 Size: 1712 4073 SexAI A'CCwGG_T Cuts at: 2808 2808 Size: 5785 SfcI C'TryA_G Cuts at: 826 1045 2395 3133 4268 4949 5140 826 Size: 219 1350 738 1135 681 191 1471 Fragments arranged by size: 1471 1350 1135 738 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2981 3889 4790 5052 820 Size: 197 926 1038 908 901 262 1553 Fragments arranged by size: 1553 1038 926 908 901 262 197 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 3040 1517 Size: 1523 4262 StyI C'CwwG_G Cuts at: 513 1065 2004 2277 2325 2971 3224 513 Size: 552 939 273 48 646 253 3074 Fragments arranged by size: 3074 939 646 552 273 253 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3812 3829 3982 4167 4612 325 Size: 1255 2232 17 153 185 445 1498 Fragments arranged by size: 2232 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4031 5743 96 Size: 274 80 33 51 1443 2054 1712 138 Fragments arranged by size: 2054 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5745 5777 5745 Size: 32 5753 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5785 XbaI T'CTAG_A Cuts at: 3121 3121 Size: 5785 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2905 2905 Size: 5785 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5785 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 3914 3914 Size: 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3593 276 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI