(Circular) (Six-base) MAPSORT of: pFN21AB9840.seq Check: 1506 from: 1 to: 5773 pFN21AB9840 5773 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3583 278 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3116 1937 Size: 1179 4594 Acc65I G'GTAC_C Cuts at: 3094 3094 Size: 5773 AclI AA'CG_TT Cuts at: 1563 3900 4273 1563 Size: 2337 373 3063 Fragments arranged by size: 3063 2337 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3836 5220 5488 1632 Size: 2204 1384 268 1917 Fragments arranged by size: 2204 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5576 AflIII A'CryG_T Cuts at: 1203 4672 5649 1203 Size: 3469 977 1327 Fragments arranged by size: 3469 1327 977 AgeI A'CCGG_T Cuts at: 4604 4604 Size: 5773 AhdI GACnn_n'nnGTC Cuts at: 4502 4502 Size: 5773 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5773 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5773 AlwNI CAG_nnn'CTG Cuts at: 3516 5088 3516 Size: 1572 4201 ApaI G_GGCC'C Cuts at: 2938 2938 Size: 5773 ApaLI G'TGCA_C Cuts at: 3830 4986 3830 Size: 1156 4617 ApoI r'AATT_y Cuts at: 1493 3082 3308 3460 5569 1493 Size: 1589 226 152 2109 1697 Fragments arranged by size: 2109 1697 1589 226 152 AseI AT'TA_AT Cuts at: 160 1032 2910 4327 160 Size: 872 1878 1417 1606 Fragments arranged by size: 1878 1606 1417 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 AvaI C'yCGr_G Cuts at: 1870 1943 3098 1870 Size: 73 1155 4545 Fragments arranged by size: 4545 1155 73 BamHI G'GATC_C Cuts at: 2118 3103 3470 2118 Size: 985 367 4421 Fragments arranged by size: 4421 985 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2314 2892 2900 3094 4549 Size: 325 217 627 527 578 8 194 1455 Cuts at: 4549 618 Size: 1842 Fragments arranged by size: 1842 1455 627 578 527 325 217 194 8 BanII G_rGCy'C Cuts at: 1391 2938 3092 1391 Size: 1547 154 4072 Fragments arranged by size: 4072 1547 154 BbeI G_GCGC'C Cuts at: 2896 2904 2896 Size: 8 5765 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4802 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3964 3998 1903 Size: 34 2027 34 3678 Fragments arranged by size: 3678 2027 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3665 4875 3665 Size: 1210 4563 BclI T'GATC_A Cuts at: 1548 2226 1548 Size: 678 5095 BfrBI ATG'CAT Cuts at: 2831 2831 Size: 5773 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4384 136 Size: 107 122 71 631 605 2712 1525 Fragments arranged by size: 2712 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5768 5768 Size: 5773 BlpI GC'TnA_GC Cuts at: 3190 3190 Size: 5773 Bme1580I G_kGCm'C Cuts at: 1790 2938 3834 4990 1790 Size: 1148 896 1156 2573 Fragments arranged by size: 2573 1156 1148 896 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5773 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2320 2512 2570 Size: 416 53 83 399 24 903 192 58 Cuts at: 2570 2951 3016 3504 4462 442 Size: 381 65 488 958 1753 Fragments arranged by size: 1753 958 903 488 416 399 381 192 83 65 58 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5773 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2530 2530 Size: 5773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 2525 2666 2773 2781 4433 1239 Size: 1022 264 141 107 8 1652 2579 Fragments arranged by size: 2579 1652 1022 264 141 107 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3898 4763 5061 3898 Size: 865 298 4610 Fragments arranged by size: 4610 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4436 882 Size: 617 2937 2219 Fragments arranged by size: 2937 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3564 493 Size: 711 2360 2702 Fragments arranged by size: 2702 2360 711 BsaBI GATnn'nnATC Cuts at: 2147 5767 2147 Size: 3620 2153 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2893 2901 3580 3962 Size: 53 83 186 936 1360 8 679 382 Cuts at: 3962 4597 275 Size: 635 1451 Fragments arranged by size: 1451 1360 936 679 635 382 186 83 53 8 BsaWI w'CCGG_w Cuts at: 2167 2768 4206 4604 4878 5025 5436 2167 Size: 601 1438 398 274 147 411 2504 Fragments arranged by size: 2504 1438 601 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2680 2710 1178 Size: 30 165 30 1277 30 4241 Fragments arranged by size: 4241 1277 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 2959 1186 Size: 955 818 4000 Fragments arranged by size: 4000 955 818 BseYI C'CCAG_C Cuts at: 4976 5472 4976 Size: 496 5277 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2626 3088 1163 Size: 573 890 462 3848 Fragments arranged by size: 3848 890 573 462 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3223 3984 5012 1540 Size: 275 186 1222 761 1028 2301 Fragments arranged by size: 2301 1222 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3834 3919 4990 3834 Size: 85 1071 4617 Fragments arranged by size: 4617 1071 85 BsmI GAATG_Cn' Cuts at: 3284 3377 3284 Size: 93 5680 BsmBI CGTCTCn'nnnn_ Cuts at: 2501 2501 Size: 5773 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2938 3092 3834 3919 4990 1391 Size: 399 1148 154 742 85 1071 2174 Fragments arranged by size: 2174 1148 1071 742 399 154 85 BspEI T'CCGG_A Cuts at: 2167 5436 2167 Size: 3269 2504 BspHI T'CATG_A Cuts at: 2538 3662 5392 2538 Size: 1124 1730 2919 Fragments arranged by size: 2919 1730 1124 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2383 2971 3128 844 Size: 1539 588 157 3489 Fragments arranged by size: 3489 1539 588 157 BsrBI CCG'CTC Cuts at: 1394 1823 2434 2701 3660 1394 Size: 429 611 267 959 3507 Fragments arranged by size: 3507 959 611 429 267 BsrDI GCAATG_nn' Cuts at: 65 1248 4268 4442 65 Size: 1183 3020 174 1396 Fragments arranged by size: 3020 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2012 2189 2317 2563 4417 4604 1700 Size: 110 202 177 128 246 1854 187 2869 Fragments arranged by size: 2869 1854 246 202 187 177 128 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5773 BssHII G'CGCG_C Cuts at: 1925 2136 1925 Size: 211 5562 BssSI C'ACGA_G Cuts at: 3833 4845 3833 Size: 1012 4761 BstAPI GCAn_nnn'nTGC Cuts at: 794 3066 794 Size: 2272 3501 BstBI TT'CG_AA Cuts at: 4577 4577 Size: 5773 BstEII G'GTnAC_C Cuts at: 1184 2296 1184 Size: 1112 4661 BstXI CCAn_nnnn'nTGG Cuts at: 2959 2959 Size: 5773 BstYI r'GATC_y Cuts at: 1972 2118 2370 2916 3103 3470 3855 3872 5324 Size: 146 252 546 187 367 385 17 1452 Cuts at: 5324 5410 5422 5768 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 546 385 367 346 252 187 146 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2303 2303 Size: 5773 BtgI C'CryG_G Cuts at: 513 1065 2004 2277 513 Size: 552 939 273 4009 Fragments arranged by size: 4009 939 552 273 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2431 487 Size: 683 56 1205 3829 Fragments arranged by size: 3829 1205 683 56 BtsI GCAGTG_nn' Cuts at: 3289 4083 4103 3289 Size: 794 20 4959 Fragments arranged by size: 4959 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5773 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5738 DraI TTT'AAA Cuts at: 3077 3077 Size: 5773 DraIII CAC_nnn'GTG Cuts at: 2328 2475 2328 Size: 147 5626 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4780 809 Size: 507 3464 1802 Fragments arranged by size: 3464 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2280 2319 2565 Size: 54 1425 50 275 18 450 39 246 Cuts at: 2565 2593 2709 3220 4109 5738 8 Size: 28 116 511 889 1629 43 Fragments arranged by size: 1629 1425 889 511 450 275 246 116 54 50 43 39 28 18 EagI C'GGCC_G Cuts at: 1537 1812 3220 1537 Size: 275 1408 4090 Fragments arranged by size: 4090 1408 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3703 1353 Size: 2350 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2909 4360 4734 4880 387 Size: 1064 138 1320 1451 374 146 1280 Fragments arranged by size: 1451 1320 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2525 2666 2773 2781 3836 4433 Size: 393 629 264 141 107 8 1055 597 Cuts at: 4433 5220 5488 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1055 787 629 597 393 268 264 141 107 8 EcoNI CCTnn'n_nnAGG Cuts at: 2974 2974 Size: 5773 EcoO109I rG'GnC_Cy Cuts at: 2289 2934 2289 Size: 645 5128 EcoRI G'AATT_C Cuts at: 3082 3460 3082 Size: 378 5395 EcoRV GAT'ATC Cuts at: 2251 2251 Size: 5773 FspI TGC'GCA Cuts at: 4279 4279 Size: 5773 HaeII r_GCGC'y Cuts at: 1678 2896 2904 4920 1678 Size: 1218 8 2016 2531 Fragments arranged by size: 2531 2016 1218 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2654 2686 2777 2809 4414 Size: 32 135 32 1082 32 91 32 1605 Cuts at: 4414 4446 4488 4520 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1605 1082 135 91 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3117 3363 669 Size: 988 281 1179 246 3079 Fragments arranged by size: 3079 1179 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5773 HpaI GTT'AAC Cuts at: 3363 3363 Size: 5773 KasI G'GCGC_C Cuts at: 2892 2900 2892 Size: 8 5765 KpnI G_GTAC'C Cuts at: 3098 3098 Size: 5773 MfeI C'AATT_G Cuts at: 3372 3372 Size: 5773 MluI A'CGCG_T Cuts at: 5649 5649 Size: 5773 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4887 5071 1305 Size: 3582 184 2007 Fragments arranged by size: 3582 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2711 10 Size: 54 1425 343 879 3072 Fragments arranged by size: 3072 1425 879 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3731 4090 4249 518 Size: 648 2565 359 159 2042 Fragments arranged by size: 2565 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2279 3189 3866 5014 5259 1606 Size: 673 910 677 1148 245 2120 Fragments arranged by size: 2120 1148 910 677 673 245 NaeI GCC'GGC Cuts at: 2319 2565 2319 Size: 246 5527 NarI GG'CG_CC Cuts at: 2893 2901 2893 Size: 8 5765 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4282 Fragments arranged by size: 4282 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5773 NgoMIV G'CCGG_C Cuts at: 2317 2563 2317 Size: 246 5527 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5773 NotI GC'GGCC_GC Cuts at: 3220 3220 Size: 5773 NsiI A_TGCA'T Cuts at: 2833 2833 Size: 5773 NspI r_CATG'y Cuts at: 1132 1229 3131 4676 1132 Size: 97 1902 1545 2229 Fragments arranged by size: 2229 1902 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5773 PciI A'CATG_T Cuts at: 4672 4672 Size: 5773 PflMI CCAn_nnn'nTGG Cuts at: 1108 2644 1108 Size: 1536 4237 PmeI GTTT'AAAC Cuts at: 3077 3077 Size: 5773 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3842 3874 5355 5387 3842 Size: 32 1481 32 4228 Fragments arranged by size: 4228 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2737 1613 Size: 1124 4649 PsiI TTA'TAA Cuts at: 3343 3343 Size: 5773 PspOMI G'GGCC_C Cuts at: 2934 2934 Size: 5773 PstI C_TGCA'G Cuts at: 830 3125 830 Size: 2295 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5773 SacII CC_GC'GG Cuts at: 2280 2280 Size: 5773 SalI G'TCGA_C Cuts at: 1936 3115 1936 Size: 1179 4594 SbfI CC_TGCA'GG Cuts at: 3125 3125 Size: 5773 ScaI AGT'ACT Cuts at: 4021 5733 4021 Size: 1712 4061 SexAI A'CCwGG_T Cuts at: 2721 2721 Size: 5773 SfcI C'TryA_G Cuts at: 826 1045 3121 4256 4937 5128 826 Size: 219 2076 1135 681 191 1471 Fragments arranged by size: 2076 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2894 2902 2894 Size: 8 5765 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5773 SmaI CCC'GGG Cuts at: 1872 3100 1872 Size: 1228 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3877 4778 5040 820 Size: 197 926 1934 901 262 1553 Fragments arranged by size: 1934 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3564 493 Size: 3071 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5773 SphI G_CATG'C Cuts at: 1132 3131 1132 Size: 1999 3774 SspI AAT'ATT Cuts at: 5 52 3697 5 Size: 47 3645 2081 Fragments arranged by size: 3645 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5773 StyI C'CwwG_G Cuts at: 513 1065 2004 2896 3212 513 Size: 552 939 892 316 3074 Fragments arranged by size: 3074 939 892 552 316 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2339 2651 2861 3800 3817 3970 4155 Size: 1255 759 312 210 939 17 153 185 Cuts at: 4155 4600 325 Size: 445 1498 Fragments arranged by size: 1498 1255 939 759 445 312 210 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4019 5731 96 Size: 274 80 33 51 1443 2042 1712 138 Fragments arranged by size: 2042 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2022 2054 2680 2712 5733 5765 2022 Size: 32 626 32 3021 32 2030 Fragments arranged by size: 3021 2030 626 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5773 XbaI T'CTAG_A Cuts at: 2659 3109 2659 Size: 450 5323 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2641 3013 2641 Size: 372 5401 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5773 XmaI C'CCGG_G Cuts at: 1870 3098 1870 Size: 1228 4545 XmnI GAAnn'nnTTC Cuts at: 3902 3902 Size: 5773 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3581 276 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstZ17I EcoICRI FalI FseI FspAI NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SrfI SwaI