Restriction map for
pFN21AB9835
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BciVI BfrI BglI BglII BlnI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV FauI FseI
FspI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 226 GTCTAC GT^[AC][GT]AC
AfaI 680 GTAC GT^AC
AlwNI 81 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 854 GGGCCC GGGCC^C
BalI 410 TGGCCA TGG^CCA
BamHI 608 GGATCC G^GATCC
BanII 854 GGGCCC G[GA]GC[CT]^C
BcgI 839 GCAACAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 125 TGATCA T^GATCA
BcnI 852 CCGGG CC^[GC]GG
BlpI 153 GCTGAGC GC^T[ACGT]AGC
BmyI 854 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 153 GCTGAGC GC^T[ACGT]AGC
BsaI 336 GGTCTC GGTCTC(1/5)
Bsp1286I 854 GGGCCC G[AGT]GC[ACT]^C
BspHI 800 TCATGA T^CATGA
BsrBI 210 CCGCTC CCGCTC(-3/-3)
BstXI 19 CCACAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 153 GCTGAGC GC^T[ACGT]AGC
CfoI 834 GCGC GCG^C
DsaI 939 CCATGG C^C[GA][CT]GG
EarI 1038 CTCTTC CTCTTC(1/4)
EcoNI 324 CCTGTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 964 ATGCAT ATGCA^T
FbaI 125 TGATCA T^GATCA
HaeII 833 AGCGCC [GA]GCGC^[CT]
HapII 852 CCGG C^CGG
HgaI 447 GCGTC GACGC(5/10)
HhaI 834 GCGC GCG^C
HinP1I 834 GCGC G^CGC
HincII 355 GTCAAC GT[CT]^[GA]AC
HindII 355 GTCAAC GT[CT]^[GA]AC
HpaII 852 CCGG C^CGG
Ksp632I 1038 CTCTTC CTCTTC(1/4)
MaeII 996 ACGT A^CGT
MluNI 410 TGGCCA TGG^CCA
MscI 410 TGGCCA TGG^CCA
MseI 689 TTAA T^TAA
MspA1I 640 CCGCTG C[AC]G^C[GT]G
MspI 852 CCGG C^CGG
NciI 852 CCGGG CC^[GC]GG
NcoI 939 CCATGG C^CATGG
NsiI 964 ATGCAT ATGCA^T
NspI 56 GCATGC [GA]CATG^[CT]
PstI 65 CTGCAG CTGCA^G
RcaI 800 TCATGA T^CATGA
RsaI 680 GTAC GT^AC
SexAI 776 ACCTGGT A^CC[AT]GGT
SmlI 949 CTCAAG C^T[CT][GA]AG
SphI 56 GCATGC GCATG^C
SspI 7 AATATT AAT^ATT
Tru9I 689 TTAA T^TAA
XcmI 866 CCACTGAGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AcsI 45 AAATTT [GA]^AATT[CT]
930 AAATTT
AluI 826 AGCT AG^CT
892 AGCT
ApoI 45 AAATTT [GA]^AATT[CT]
930 AAATTT
BfaI 94 CTAG C^TAG
142 CTAG
BsiEI 207 CGGCCG CG[GA][CT]^CG
849 CGGCCG
BsmFI 483 GGGAC GGGAC(10/14)
501 GTCCC
BspMI 63 ACCTGC ACCTGC(4/8)
230 ACCTGC
EagI 207 CGGCCG C^GGCCG
849 CGGCCG
EclXI 207 CGGCCG C^GGCCG
849 CGGCCG
Eco52I 207 CGGCCG C^GGCCG
849 CGGCCG
MaeI 94 CTAG C^TAG
142 CTAG
PpuMI 566 AGGTCCT [GA]G^G[AT]CC[CT]
805 AGGACCC
SfcI 65 CTGCAG C^T[GA][CT]AG
363 CTACAG
Tsp45I 217 GTCAC ^GT[GC]AC
449 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AlwI AvaII BbvI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MflI MnlI MslI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
TseI Tsp509I TspRI XhoII