(Circular) (Six-base) MAPSORT of: pFN21AB9835.seq Check: 173 from: 1 to: 5755 pFN21AB9835 5755 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2077 2077 Size: 5755 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2232 3098 1937 Size: 295 866 4594 Fragments arranged by size: 4594 866 295 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2372 3818 5202 5470 1632 Size: 740 1446 1384 268 1917 Fragments arranged by size: 1917 1446 1384 740 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 4654 5631 1203 Size: 3451 977 1327 Fragments arranged by size: 3451 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 2029 1166 Size: 863 4892 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5755 AlwNI CAG_nnn'CTG Cuts at: 2091 3498 5070 2091 Size: 1407 1572 2776 Fragments arranged by size: 2776 1572 1407 ApaI G_GGCC'C Cuts at: 2863 2863 Size: 5755 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 2050 2935 3064 3290 3442 5551 1493 Size: 557 885 129 226 152 2109 1697 Fragments arranged by size: 2109 1697 885 557 226 152 129 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 3080 1870 Size: 73 1137 4545 Fragments arranged by size: 4545 1137 73 BamHI G'GATC_C Cuts at: 2613 3085 3452 2613 Size: 472 367 4916 Fragments arranged by size: 4916 472 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3076 4531 618 Size: 325 217 627 1289 1455 1842 Fragments arranged by size: 1842 1455 1289 627 325 217 BanII G_rGCy'C Cuts at: 1391 2863 3074 1391 Size: 1472 211 4072 Fragments arranged by size: 4072 1472 211 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4784 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2833 2867 3946 3980 1903 Size: 34 896 34 1079 34 3678 Fragments arranged by size: 3678 1079 896 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3647 4857 3647 Size: 1210 4545 BclI T'GATC_A Cuts at: 1548 2130 1548 Size: 582 5173 BfrBI ATG'CAT Cuts at: 2971 2971 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4366 136 Size: 107 122 71 631 605 2694 1525 Fragments arranged by size: 2694 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 2159 3172 2159 Size: 1013 4742 Bme1580I G_kGCm'C Cuts at: 1790 2863 3816 4972 1790 Size: 1073 953 1156 2573 Fragments arranged by size: 2573 1156 1073 953 BmgBI CAC'GTC Cuts at: 1318 3002 1318 Size: 1684 4071 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3486 4444 442 Size: 416 53 83 399 24 2069 958 1753 Fragments arranged by size: 2069 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4415 1239 Size: 3176 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2939 3880 4745 5043 2939 Size: 941 865 298 3651 Fragments arranged by size: 3651 941 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2347 4418 882 Size: 617 848 2071 2219 Fragments arranged by size: 2219 2071 848 617 BsaAI yAC'GTr Cuts at: 493 1204 3546 493 Size: 711 2342 2702 Fragments arranged by size: 2702 2342 711 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3562 3944 4579 275 Size: 53 83 186 936 2029 382 635 1451 Fragments arranged by size: 2029 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4188 4586 4860 5007 5418 4188 Size: 398 274 147 411 4525 Fragments arranged by size: 4525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5530 Fragments arranged by size: 5530 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5755 BseYI C'CCAG_C Cuts at: 2167 2484 4958 5454 2167 Size: 317 2474 496 2468 Fragments arranged by size: 2474 2468 496 317 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2215 2857 3205 3966 4994 1540 Size: 275 186 214 642 348 761 1028 2301 Fragments arranged by size: 2301 1028 761 642 348 275 214 186 BsiHKAI G_wGCw'C Cuts at: 3816 3901 4972 3816 Size: 85 1071 4599 Fragments arranged by size: 4599 1071 85 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2863 3074 3816 3901 4972 1391 Size: 399 1073 211 742 85 1071 2174 Fragments arranged by size: 2174 1073 1071 742 399 211 85 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 2805 3644 5374 2805 Size: 839 1730 3186 Fragments arranged by size: 3186 1730 839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2077 2244 3110 844 Size: 1233 167 866 3489 Fragments arranged by size: 3489 1233 866 167 BsrBI CCG'CTC Cuts at: 1394 1823 2217 3642 1394 Size: 429 394 1425 3507 Fragments arranged by size: 3507 1425 429 394 BsrDI GCAATG_nn' Cuts at: 65 1248 4250 4424 65 Size: 1183 3002 174 1396 Fragments arranged by size: 3002 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4399 4586 1700 Size: 110 2589 187 2869 Fragments arranged by size: 2869 2589 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5755 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstXI CCAn_nnnn'nTGG Cuts at: 2031 2031 Size: 5755 BstYI r'GATC_y Cuts at: 1972 2020 2613 2958 3085 3452 3837 3854 5306 Size: 48 593 345 127 367 385 17 1452 Cuts at: 5306 5392 5404 5750 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 593 385 367 346 345 127 86 48 17 12 BtgI C'CryG_G Cuts at: 513 1065 2944 513 Size: 552 1879 3324 Fragments arranged by size: 3324 1879 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2119 487 Size: 683 56 893 4123 Fragments arranged by size: 4123 893 683 56 BtsI GCAGTG_nn' Cuts at: 3271 4065 4085 3271 Size: 794 20 4941 Fragments arranged by size: 4941 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2212 2253 2415 Size: 54 1425 50 275 18 382 41 162 Cuts at: 2415 2854 2929 3202 4091 5720 8 Size: 439 75 273 889 1629 43 Fragments arranged by size: 1629 1425 889 439 382 275 273 162 75 54 50 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 2212 2854 3202 1537 Size: 275 400 642 348 4090 Fragments arranged by size: 4090 642 400 348 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3049 3685 1353 Size: 1696 636 3423 Fragments arranged by size: 3423 1696 636 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2516 2614 4342 4716 4862 387 Size: 1064 138 927 98 1728 374 146 1280 Fragments arranged by size: 1728 1280 1064 927 374 146 138 98 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2372 3818 4415 5202 5470 1239 Size: 393 740 1446 597 787 268 1524 Fragments arranged by size: 1524 1446 787 740 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2333 2333 Size: 5755 EcoO109I rG'GnC_Cy Cuts at: 2572 2811 2860 2572 Size: 239 49 5467 Fragments arranged by size: 5467 239 49 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2473 3010 2473 Size: 537 5218 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 2842 4902 1678 Size: 1164 2060 2531 Fragments arranged by size: 2531 2060 1164 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2605 2637 4396 4428 4470 Size: 32 135 32 1033 32 1759 32 42 Cuts at: 4470 4502 1373 Size: 32 2626 Fragments arranged by size: 2626 1759 1033 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2362 3099 3345 669 Size: 988 281 424 737 246 3079 Fragments arranged by size: 3079 988 737 424 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3072 4869 5053 1305 Size: 1767 1797 184 2007 Fragments arranged by size: 2007 1797 1767 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2417 10 Size: 54 1425 343 585 3348 Fragments arranged by size: 3348 1425 585 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2029 2060 2329 2399 2804 3713 4072 Size: 648 863 31 269 70 405 909 359 Cuts at: 4072 4231 518 Size: 159 2042 Fragments arranged by size: 2042 909 863 648 405 359 269 159 70 31 MspA1I CmG'CkG Cuts at: 1606 2647 3171 3848 4996 5241 1606 Size: 1041 524 677 1148 245 2120 Fragments arranged by size: 2120 1148 1041 677 524 245 NcoI C'CATG_G Cuts at: 513 1065 2944 513 Size: 552 1879 3324 Fragments arranged by size: 3324 1879 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NsiI A_TGCA'T Cuts at: 2973 2973 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 2065 3113 4658 1132 Size: 97 836 1048 1545 2229 Fragments arranged by size: 2229 1545 1048 836 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5755 PfoI T'CCnGG_A Cuts at: 2823 2823 Size: 5755 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2572 2811 2572 Size: 239 5516 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PspOMI G'GGCC_C Cuts at: 2859 2859 Size: 5755 PstI C_TGCA'G Cuts at: 830 2074 3107 830 Size: 1244 1033 3478 Fragments arranged by size: 3478 1244 1033 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SexAI A'CCwGG_T Cuts at: 2781 2781 Size: 5755 SfcI C'TryA_G Cuts at: 826 1045 2070 2368 3103 4238 4919 5110 826 Size: 219 1025 298 735 1135 681 191 1471 Fragments arranged by size: 1471 1135 1025 735 681 298 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2954 3859 4760 5022 820 Size: 197 926 1011 905 901 262 1553 Fragments arranged by size: 1553 1011 926 905 901 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 2065 3113 1132 Size: 933 1048 3774 Fragments arranged by size: 3774 1048 933 SspI AAT'ATT Cuts at: 5 52 2014 3679 5 Size: 47 1962 1665 2081 Fragments arranged by size: 2081 1962 1665 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5755 StyI C'CwwG_G Cuts at: 513 1065 2250 2298 2944 3194 513 Size: 552 1185 48 646 250 3074 Fragments arranged by size: 3074 1185 646 552 250 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3782 3799 3952 4137 4582 325 Size: 1255 2202 17 153 185 445 1498 Fragments arranged by size: 2202 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4001 5713 96 Size: 274 80 33 51 1443 2024 1712 138 Fragments arranged by size: 2024 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5715 5747 5715 Size: 32 5723 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2878 2878 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI