Restriction map for
pFN21AB9834
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BamHI BbrPI BbvCI
BcgI BciVI BfrI BsaAI BsaBI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspHI BspLU11I BsrDI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 943 GACGTC GACGT^C
AccIII 32 TCCGGA T^CCGGA
AlwI 1008 GGATC GGATC(4/5)
AlwNI 190 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 472 CCTGAGG CC^T[ACGT]AGG
Aor51HI 953 AGCGCT AGC^GCT
ApaI 125 GGGCCC GGGCC^C
AvrII 399 CCTAGG C^CTAGG
BalI 251 TGGCCA TGG^CCA
BbsI 907 GTCTTC GAAGAC(2/6)
BclI 623 TGATCA T^GATCA
BglI 138 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 681 AGATCT A^GATCT
BlnI 399 CCTAGG C^CTAGG
BlpI 313 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 313 GCTGAGC GC^T[ACGT]AGC
BpuAI 907 GTCTTC GAAGAC(2/6)
BsaI 963 GAGACC GGTCTC(1/5)
BsaWI 32 TCCGGA [AT]^CCGG[AT]
BseAI 32 TCCGGA T^CCGGA
Bsp1407I 221 TGTACA T^GTACA
BspEI 32 TCCGGA T^CCGGA
BsrBI 844 GAGCGG CCGCTC(-3/-3)
BsrGI 221 TGTACA T^GTACA
BssHII 989 GCGCGC G^CGCGC
BstXI 407 CCATCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 472 CCTGAGG CC^T[ACGT]AGG
CelII 313 GCTGAGC GC^T[ACGT]AGC
DraII 125 GGGCCCC [GA]G^G[ACGT]CC[CT]
Eco47III 953 AGCGCT AGC^GCT
Eco81I 472 CCTGAGG CC^T[ACGT]AGG
EcoNI 712 CCTCTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 125 GGGCCCC [GA]G^G[ACGT]CC[CT]
FbaI 623 TGATCA T^GATCA
HinfI 916 GACTC G^A[ACGT]TC
HphI 1013 TCACC GGTGA(8/7)
MluNI 251 TGGCCA TGG^CCA
MroI 32 TCCGGA T^CCGGA
MscI 251 TGGCCA TGG^CCA
MseI 517 TTAA T^TAA
MslI 408 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 414 CATG CATG^
PflMI 161 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 916 GACTC GAGTC(4/5)
SapI 567 GCTCTTC GCTCTTC(1/4)
ScaI 539 AGTACT AGT^ACT
SfaNI 292 GCATC GCATC(5/9)
SmaI 925 CCCGGG CCC^GGG
SmlI 535 CTCAAG C^T[CT][GA]AG
SspBI 221 TGTACA T^GTACA
TaqI 911 TCGA T^CGA
Tru9I 517 TTAA T^TAA
Tsp509I 648 AATT ^AATT
TthHB8I 911 TCGA T^CGA
Van91I 161 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 444 CCAAGTCATCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 925 CCCGGG C^CCGGG
XmaI 925 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Alw44I 273 GTGCAC G^TGCAC
592 GTGCAC
ApaLI 273 GTGCAC G^TGCAC
592 GTGCAC
AvaI 122 CTCGGG C^[CT]CG[GA]G
925 CCCGGG
BanI 457 GGCGCC G^G[CT][GA]CC
828 GGCGCC
BbeI 457 GGCGCC GGCGC^C
828 GGCGCC
BsmAI 963 GAGAC GTCTC(1/5)
533 GTCTC
BsoBI 122 CTCGGG C^[CT]CG[GA]G
925 CCCGGG
BsrFI 143 GCCGGC [GA]^CCGG[CT]
463 GCCGGC
BsrI 167 ACTGG ACTGG(1/-1)
505 ACTGG
BstF5I 948 CATCC GGATG(2/0)
636 GGATG
BstNI 12 CCTGG CC^[AT]GG
152 CCTGG
BstYI 681 AGATCT [GA]^GATC[CT]
1008 GGATCT
Cfr10I 143 GCCGGC [GA]^CCGG[CT]
463 GCCGGC
DsaI 130 CCGCGG C^C[GA][CT]GG
237 CCGCGG
EaeI 251 TGGCCA [CT]^GGCC[GA]
465 CGGCCA
EarI 116 CTCTTC CTCTTC(1/4)
568 CTCTTC
EcoRII 12 CCTGG ^CC[AT]GG
152 CCTGG
EcoT14I 399 CCTAGG C^C[AT][AT]GG
936 CCTTGG
FokI 948 CATCC GGATG(9/13)
636 GGATG
KasI 457 GGCGCC G^GCGCC
828 GGCGCC
Ksp632I 116 CTCTTC CTCTTC(1/4)
568 CTCTTC
KspI 130 CCGCGG CCGC^GG
237 CCGCGG
MflI 681 AGATCT [GA]^GATC[CT]
1008 GGATCT
MvaI 12 CCTGG CC^[AT]GG
152 CCTGG
NaeI 143 GCCGGC GCC^GGC
463 GCCGGC
NarI 457 GGCGCC GG^CGCC
828 GGCGCC
NgoMIV 143 GCCGGC G^CCGGC
463 GCCGGC
PstI 69 CTGCAG CTGCA^G
187 CTGCAG
SacI 316 GAGCTC GAGCT^C
745 GAGCTC
SacII 130 CCGCGG CCGC^GG
237 CCGCGG
SfcI 69 CTGCAG C^T[GA][CT]AG
187 CTGCAG
SfoI 457 GGCGCC GGC^GCC
828 GGCGCC
StyI 399 CCTAGG C^C[AT][AT]GG
936 CCTTGG
XhoII 681 AGATCT [GA]^GATC[CT]
1008 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BspMI BssKI BstUI Cac8I CfoI
Cfr13I DdeI DpnII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvnI MwoI NciI
NdeII NlaIV RsaI Sau3AI Sau96I
ScrFI TseI Tsp45I TspRI