Restriction map for
pFN21AB9834
(Linear) (Six-base) MAPSORT of: KIBB9834.seq Check: 6908 from: 1 to: 1038
KIBB9834.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 165 1038
Size: 165 873
AatII G_ACGT'C
Cuts at: 0 947 1038
Size: 947 91
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1038
Size: 625 413
AfeI AGC'GCT
Cuts at: 0 955 1038
Size: 955 83
AlwNI CAG_nnn'CTG
Cuts at: 0 195 1038
Size: 195 843
ApaI G_GGCC'C
Cuts at: 0 129 1038
Size: 129 909
ApaLI G'TGCA_C
Cuts at: 0 273 592 1038
Size: 273 319 446
Fragments arranged by size:
446 319 273
AvaI C'yCGr_G
Cuts at: 0 122 925 1038
Size: 122 803 113
Fragments arranged by size:
803 122 113
AvrII C'CTAG_G
Cuts at: 0 399 1038
Size: 399 639
BanI G'GyrC_C
Cuts at: 0 457 828 1038
Size: 457 371 210
Fragments arranged by size:
457 371 210
BanII G_rGCy'C
Cuts at: 0 129 320 749 1038
Size: 129 191 429 289
Fragments arranged by size:
429 289 191 129
BbeI G_GCGC'C
Cuts at: 0 461 832 1038
Size: 461 371 206
Fragments arranged by size:
461 371 206
BbsI GAAGACnn'nnnn_
Cuts at: 0 900 1038
Size: 900 138
BclI T'GATC_A
Cuts at: 0 623 1038
Size: 623 415
BglI GCCn_nnn'nGGC
Cuts at: 0 144 1038
Size: 144 894
BglII A'GATC_T
Cuts at: 0 681 1038
Size: 681 357
BlpI GC'TnA_GC
Cuts at: 0 314 1038
Size: 314 724
Bme1580I G_kGCm'C
Cuts at: 0 129 277 596 1038
Size: 129 148 319 442
Fragments arranged by size:
442 319 148 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 177 1038
Size: 177 861
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 527 981 1038
Size: 224 303 454 57
Fragments arranged by size:
454 303 224 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1038
Size: 520 518
BsaI GGTCTCn'nnnn_
Cuts at: 0 957 1038
Size: 957 81
BsaHI Gr'CG_yC
Cuts at: 0 458 769 829 944 1038
Size: 458 311 60 115 94
Fragments arranged by size:
458 311 115 94 60
BsaWI w'CCGG_w
Cuts at: 0 32 1038
Size: 32 1006
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 163 193 749 779 1038
Size: 163 30 556 30 259
Fragments arranged by size:
556 259 163 30 30
BseYI C'CCAG_C
Cuts at: 0 195 967 1038
Size: 195 772 71
Fragments arranged by size:
772 195 71
BsiHKAI G_wGCw'C
Cuts at: 0 160 277 320 596 749 1038
Size: 160 117 43 276 153 289
Fragments arranged by size:
289 276 160 153 117 43
Bsp1286I G_dGCh'C
Cuts at: 0 129 160 277 320 596 749 1038
Size: 129 31 117 43 276 153 289
Fragments arranged by size:
289 276 153 129 117 43 31
BspEI T'CCGG_A
Cuts at: 0 32 1038
Size: 32 1006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 99 165 890 1038
Size: 99 66 725 148
Fragments arranged by size:
725 148 99 66
BsrBI CCG'CTC
Cuts at: 0 846 1038
Size: 846 192
BsrFI r'CCGG_y
Cuts at: 0 143 463 1038
Size: 143 320 575
Fragments arranged by size:
575 320 143
BsrGI T'GTAC_A
Cuts at: 0 221 1038
Size: 221 817
BssHII G'CGCG_C
Cuts at: 0 989 1038
Size: 989 49
BstXI CCAn_nnnn'nTGG
Cuts at: 0 414 1038
Size: 414 624
BstYI r'GATC_y
Cuts at: 0 681 1008 1038
Size: 681 327 30
Fragments arranged by size:
681 327 30
Bsu36I CC'TnA_GG
Cuts at: 0 473 1038
Size: 473 565
BtgI C'CryG_G
Cuts at: 0 130 237 1038
Size: 130 107 801
Fragments arranged by size:
801 130 107
BtsI GCAGTG_nn'
Cuts at: 0 495 1038
Size: 495 543
EaeI y'GGCC_r
Cuts at: 0 251 465 1038
Size: 251 214 573
Fragments arranged by size:
573 251 214
EarI CTCTTCn'nnn_
Cuts at: 0 122 574 1038
Size: 122 452 464
Fragments arranged by size:
464 452 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 315 1038
Size: 315 723
EcoICRI GAG'CTC
Cuts at: 0 318 747 1038
Size: 318 429 291
Fragments arranged by size:
429 318 291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 527 625 981 1038
Size: 224 303 98 356 57
Fragments arranged by size:
356 303 224 98 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 716 1038
Size: 716 322
EcoO109I rG'GnC_Cy
Cuts at: 0 126 1038
Size: 126 912
HaeII r_GCGC'y
Cuts at: 0 461 832 936 957 1038
Size: 461 371 104 21 81
Fragments arranged by size:
461 371 104 81 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 1038
Size: 1000 32 6
Fragments arranged by size:
1000 32 6
KasI G'GCGC_C
Cuts at: 0 457 828 1038
Size: 457 371 210
Fragments arranged by size:
457 371 210
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 1038
Size: 656 382
MscI TGG'CCA
Cuts at: 0 253 1038
Size: 253 785
MslI CAynn'nnrTG
Cuts at: 0 412 1038
Size: 412 626
MspA1I CmG'CkG
Cuts at: 0 132 239 901 1038
Size: 132 107 662 137
Fragments arranged by size:
662 137 132 107
NaeI GCC'GGC
Cuts at: 0 145 465 1038
Size: 145 320 573
Fragments arranged by size:
573 320 145
NarI GG'CG_CC
Cuts at: 0 458 829 1038
Size: 458 371 209
Fragments arranged by size:
458 371 209
NgoMIV G'CCGG_C
Cuts at: 0 143 463 1038
Size: 143 320 575
Fragments arranged by size:
575 320 143
PflMI CCAn_nnn'nTGG
Cuts at: 0 167 1038
Size: 167 871
PspOMI G'GGCC_C
Cuts at: 0 125 1038
Size: 125 913
PstI C_TGCA'G
Cuts at: 0 73 191 1038
Size: 73 118 847
Fragments arranged by size:
847 118 73
SacI G_AGCT'C
Cuts at: 0 320 749 1038
Size: 320 429 289
Fragments arranged by size:
429 320 289
SacII CC_GC'GG
Cuts at: 0 133 240 1038
Size: 133 107 798
Fragments arranged by size:
798 133 107
SapI GCTCTTCn'nnn_
Cuts at: 0 574 1038
Size: 574 464
ScaI AGT'ACT
Cuts at: 0 541 1038
Size: 541 497
SfcI C'TryA_G
Cuts at: 0 69 187 1038
Size: 69 118 851
Fragments arranged by size:
851 118 69
SfoI GGC'GCC
Cuts at: 0 459 830 1038
Size: 459 371 208
Fragments arranged by size:
459 371 208
SmaI CCC'GGG
Cuts at: 0 927 1038
Size: 927 111
SmlI C'TyrA_G
Cuts at: 0 535 1038
Size: 535 503
StyI C'CwwG_G
Cuts at: 0 399 936 1038
Size: 399 537 102
Fragments arranged by size:
537 399 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 745 1038
Size: 745 293
TatI w'GTAC_w
Cuts at: 0 221 539 1038
Size: 221 318 499
Fragments arranged by size:
499 318 221
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 163 195 749 781 1038
Size: 163 32 554 32 257
Fragments arranged by size:
554 257 163 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 451 1038
Size: 451 587
XmaI C'CCGG_G
Cuts at: 0 925 1038
Size: 925 113
ZraI GAC'GTC
Cuts at: 0 945 1038
Size: 945 93
Enzymes that do cut:
AarI AatII AcuI AfeI AlwNI ApaI ApaLI AvaI
AvrII BanI BanII BbeI BbsI BclI BglI BglII
BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaHI BsaWI
BsaXI BseYI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI
BsrGI BssHII BstXI BstYI Bsu36I BtgI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
PflMI PspOMI PstI SacI SacII SapI ScaI SfcI
SfoI SmaI SmlI StyI TaqII TatI TstI XcmI
XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI BaeI BamHI
BbvCI BcgI BciVI BfrBI BmgBI BmtI BplI Bpu10I
BsaAI BsaBI BseRI BsgI BsiEI BsiWI BsmI BsmBI
BspHI BsrDI BssSI BstAPI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SalI SanDI SbfI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmnI