(Circular) (Six-base) MAPSORT of: pFN21AB9827.seq Check: 5118 from: 1 to: 5680 pFN21AB9827 5680 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2125 2125 Size: 5680 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3490 278 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3023 1937 Size: 1086 4594 Acc65I G'GTAC_C Cuts at: 3001 3001 Size: 5680 AclI AA'CG_TT Cuts at: 1563 3807 4180 1563 Size: 2244 373 3063 Fragments arranged by size: 3063 2244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2117 2204 2554 2663 2785 3743 5127 5395 Size: 485 87 350 109 122 958 1384 268 Cuts at: 5395 1632 Size: 1917 Fragments arranged by size: 1917 1384 958 485 350 268 122 109 87 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5483 AflIII A'CryG_T Cuts at: 1203 4579 5556 1203 Size: 3376 977 1327 Fragments arranged by size: 3376 1327 977 AgeI A'CCGG_T Cuts at: 4511 4511 Size: 5680 AhdI GACnn_n'nnGTC Cuts at: 4409 4409 Size: 5680 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5680 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2645 2897 1184 Size: 1461 252 3967 Fragments arranged by size: 3967 1461 252 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2132 2164 2132 Size: 32 5648 AlwNI CAG_nnn'CTG Cuts at: 2284 2299 2577 2641 2662 2931 3423 4995 2284 Size: 15 278 64 21 269 492 1572 2969 Fragments arranged by size: 2969 1572 492 278 269 64 21 15 ApaLI G'TGCA_C Cuts at: 2813 3737 4893 2813 Size: 924 1156 3600 Fragments arranged by size: 3600 1156 924 ApoI r'AATT_y Cuts at: 1493 2989 3215 3367 5476 1493 Size: 1496 226 152 2109 1697 Fragments arranged by size: 2109 1697 1496 226 152 AscI GG'CGCG_CC Cuts at: 2416 2416 Size: 5680 AseI AT'TA_AT Cuts at: 160 1032 4234 160 Size: 872 3202 1606 Fragments arranged by size: 3202 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 AvaI C'yCGr_G Cuts at: 1870 1943 2587 2948 3005 1870 Size: 73 644 361 57 4545 Fragments arranged by size: 4545 644 361 73 57 BamHI G'GATC_C Cuts at: 3010 3377 3010 Size: 367 5313 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 3001 4456 618 Size: 325 217 627 234 980 1455 1842 Fragments arranged by size: 1842 1455 980 627 325 234 217 BanII G_rGCy'C Cuts at: 1391 2999 1391 Size: 1608 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4709 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3871 3905 1903 Size: 34 1934 34 3678 Fragments arranged by size: 3678 1934 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3572 4782 3572 Size: 1210 4470 BclI T'GATC_A Cuts at: 1548 1548 Size: 5680 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2174 2402 4291 Size: 107 122 71 631 605 502 228 1889 Cuts at: 4291 136 Size: 1525 Fragments arranged by size: 1889 1525 631 605 502 228 122 107 71 BglII A'GATC_T Cuts at: 2352 5675 2352 Size: 3323 2357 BlpI GC'TnA_GC Cuts at: 2834 3097 2834 Size: 263 5417 Bme1580I G_kGCm'C Cuts at: 1790 2024 2817 3741 4897 1790 Size: 234 793 924 1156 2573 Fragments arranged by size: 2573 1156 924 793 234 BmgBI CAC'GTC Cuts at: 1318 2446 2824 1318 Size: 1128 378 4174 Fragments arranged by size: 4174 1128 378 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2587 3411 4369 Size: 416 53 83 399 24 1170 824 958 Cuts at: 4369 442 Size: 1753 Fragments arranged by size: 1753 1170 958 824 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5680 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2704 2704 Size: 5680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2306 2699 4340 1239 Size: 1067 393 1641 2579 Fragments arranged by size: 2579 1641 1067 393 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2042 2111 3805 4670 4968 2042 Size: 69 1694 865 298 2754 Fragments arranged by size: 2754 1694 865 298 69 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2111 4343 882 Size: 617 612 2232 2219 Fragments arranged by size: 2232 2219 617 612 BsaAI yAC'GTr Cuts at: 493 1204 3471 493 Size: 711 2267 2702 Fragments arranged by size: 2702 2267 711 BsaBI GATnn'nnATC Cuts at: 5674 5674 Size: 5680 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2166 2841 3487 3869 Size: 53 83 186 936 633 675 646 382 Cuts at: 3869 4504 275 Size: 635 1451 Fragments arranged by size: 1451 936 675 646 635 633 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4113 4511 4785 4932 5343 4113 Size: 398 274 147 411 4450 Fragments arranged by size: 4450 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5455 Fragments arranged by size: 5455 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2129 2303 2495 2498 2501 2519 2567 Size: 859 84 174 192 3 3 18 48 Cuts at: 2567 1186 Size: 4299 Fragments arranged by size: 4299 859 192 174 84 48 18 3 3 BseYI C'CCAG_C Cuts at: 2931 4883 5379 2931 Size: 1952 496 3232 Fragments arranged by size: 3232 1952 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2651 1163 Size: 573 915 4192 Fragments arranged by size: 4192 915 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2406 2476 3130 3891 4919 1540 Size: 275 186 405 70 654 761 1028 2301 Fragments arranged by size: 2301 1028 761 654 405 275 186 70 BsiHKAI G_wGCw'C Cuts at: 2817 3741 3826 4897 2817 Size: 924 85 1071 3600 Fragments arranged by size: 3600 1071 924 85 BsmI GAATG_Cn' Cuts at: 3191 3284 3191 Size: 93 5587 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2817 2999 3741 3826 4897 1391 Size: 399 234 793 182 742 85 1071 2174 Fragments arranged by size: 2174 1071 793 742 399 234 182 85 BspEI T'CCGG_A Cuts at: 5343 5343 Size: 5680 BspHI T'CATG_A Cuts at: 3569 5299 3569 Size: 1730 3950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2125 3035 844 Size: 1281 910 3489 Fragments arranged by size: 3489 1281 910 BsrBI CCG'CTC Cuts at: 1394 1823 2260 2311 2790 2797 3567 1394 Size: 429 437 51 479 7 770 3507 Fragments arranged by size: 3507 770 479 437 429 51 7 BsrDI GCAATG_nn' Cuts at: 65 1248 4175 4349 65 Size: 1183 2927 174 1396 Fragments arranged by size: 2927 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2493 2595 2693 4324 4511 1700 Size: 110 683 102 98 1631 187 2869 Fragments arranged by size: 2869 1631 683 187 110 102 98 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5680 BssHII G'CGCG_C Cuts at: 1925 2416 1925 Size: 491 5189 BssSI C'ACGA_G Cuts at: 3740 4752 3740 Size: 1012 4668 BstAPI GCAn_nnn'nTGC Cuts at: 794 2042 2299 2662 794 Size: 1248 257 363 3812 Fragments arranged by size: 3812 1248 363 257 BstBI TT'CG_AA Cuts at: 4484 4484 Size: 5680 BstEII G'GTnAC_C Cuts at: 1184 2206 2563 1184 Size: 1022 357 4301 Fragments arranged by size: 4301 1022 357 BstXI CCAn_nnnn'nTGG Cuts at: 2197 2197 Size: 5680 BstYI r'GATC_y Cuts at: 1972 2352 3010 3377 3762 3779 5231 5317 5329 Size: 380 658 367 385 17 1452 86 12 Cuts at: 5329 5675 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 658 385 380 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2169 2847 513 Size: 552 939 165 678 3346 Fragments arranged by size: 3346 939 678 552 165 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2092 2752 487 Size: 683 56 866 660 3415 Fragments arranged by size: 3415 866 683 660 56 BtsI GCAGTG_nn' Cuts at: 3196 3990 4010 3196 Size: 794 20 4866 Fragments arranged by size: 4866 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5645 DraI TTT'AAA Cuts at: 2984 2984 Size: 5680 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4687 809 Size: 507 3371 1802 Fragments arranged by size: 3371 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2268 2403 3127 Size: 54 1425 50 275 18 438 135 724 Cuts at: 3127 4016 5645 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 724 438 275 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2403 3127 1537 Size: 275 591 724 4090 Fragments arranged by size: 4090 724 591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2804 3610 1353 Size: 1451 806 3423 Fragments arranged by size: 3423 1451 806 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2680 4267 4641 4787 387 Size: 1064 138 1091 1587 374 146 1280 Fragments arranged by size: 1587 1280 1091 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2204 2306 2554 2663 2699 2785 Size: 393 485 87 102 248 109 36 86 Cuts at: 2785 3743 4340 5127 5395 1239 Size: 958 597 787 268 1524 Fragments arranged by size: 1524 958 787 597 485 393 268 248 109 102 87 86 36 EcoNI CCTnn'n_nnAGG Cuts at: 2645 2645 Size: 5680 EcoO109I rG'GnC_Cy Cuts at: 2239 2725 2239 Size: 486 5194 EcoRI G'AATT_C Cuts at: 2989 3367 2989 Size: 378 5302 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2932 2932 Size: 5680 FspI TGC'GCA Cuts at: 2615 2666 2888 4186 2615 Size: 51 222 1298 4109 Fragments arranged by size: 4109 1298 222 51 HaeII r_GCGC'y Cuts at: 1678 2278 2654 4827 1678 Size: 600 376 2173 2531 Fragments arranged by size: 2531 2173 600 376 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 4321 4353 4395 Size: 32 135 32 599 32 2118 32 42 Cuts at: 4395 4427 1373 Size: 32 2626 Fragments arranged by size: 2626 2118 599 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3024 3270 669 Size: 988 281 1086 246 3079 Fragments arranged by size: 3079 1086 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5680 HpaI GTT'AAC Cuts at: 3270 3270 Size: 5680 KpnI G_GTAC'C Cuts at: 3005 3005 Size: 5680 MfeI C'AATT_G Cuts at: 3279 3279 Size: 5680 MluI A'CGCG_T Cuts at: 5556 5556 Size: 5680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4794 4978 1305 Size: 3489 184 2007 Fragments arranged by size: 3489 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2270 10 Size: 54 1425 343 438 3420 Fragments arranged by size: 3420 1425 438 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3638 3997 4156 518 Size: 648 2472 359 159 2042 Fragments arranged by size: 2472 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2077 2266 2296 2317 2332 2892 2931 3096 Size: 471 189 30 21 15 560 39 165 Cuts at: 3096 3773 4921 5166 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 560 471 245 189 165 39 30 21 15 NaeI GCC'GGC Cuts at: 2695 2695 Size: 5680 NcoI C'CATG_G Cuts at: 513 1065 2004 2847 513 Size: 552 939 843 3346 Fragments arranged by size: 3346 939 843 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5680 NgoMIV G'CCGG_C Cuts at: 2693 2693 Size: 5680 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5680 NotI GC'GGCC_GC Cuts at: 3127 3127 Size: 5680 NspI r_CATG'y Cuts at: 1132 1229 3038 4583 1132 Size: 97 1809 1545 2229 Fragments arranged by size: 2229 1809 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5680 PciI A'CATG_T Cuts at: 4579 4579 Size: 5680 PflMI CCAn_nnn'nTGG Cuts at: 1108 2284 2931 1108 Size: 1176 647 3857 Fragments arranged by size: 3857 1176 647 PfoI T'CCnGG_A Cuts at: 2676 2676 Size: 5680 PmeI GTTT'AAAC Cuts at: 2984 2984 Size: 5680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3749 3781 5262 5294 3749 Size: 32 1481 32 4135 Fragments arranged by size: 4135 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2725 2725 Size: 5680 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5680 PsiI TTA'TAA Cuts at: 3250 3250 Size: 5680 PstI C_TGCA'G Cuts at: 830 2304 2658 3032 830 Size: 1474 354 374 3478 Fragments arranged by size: 3478 1474 374 354 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5680 PvuII CAG'CTG Cuts at: 2266 2892 2266 Size: 626 5054 SalI G'TCGA_C Cuts at: 1936 3022 1936 Size: 1086 4594 SapI GCTCTTCn'nnn_ Cuts at: 2804 2804 Size: 5680 SbfI CC_TGCA'GG Cuts at: 3032 3032 Size: 5680 ScaI AGT'ACT Cuts at: 3928 5640 3928 Size: 1712 3968 SfcI C'TryA_G Cuts at: 826 1045 2300 2654 3028 4163 4844 5035 826 Size: 219 1255 354 374 1135 681 191 1471 Fragments arranged by size: 1471 1255 1135 681 374 354 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2402 2402 Size: 5680 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 SgrAI Cr'CCGG_yG Cuts at: 1700 2595 2693 1700 Size: 895 98 4687 Fragments arranged by size: 4687 895 98 SmaI CCC'GGG Cuts at: 1872 3007 1872 Size: 1135 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2057 2126 3784 4685 4947 820 Size: 197 926 114 69 1658 901 262 1553 Fragments arranged by size: 1658 1553 926 901 262 197 114 69 SnaBI TAC'GTA Cuts at: 493 3471 493 Size: 2978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5680 SphI G_CATG'C Cuts at: 1132 3038 1132 Size: 1906 3774 SspI AAT'ATT Cuts at: 5 52 3604 5 Size: 47 3552 2081 Fragments arranged by size: 3552 2081 47 StuI AGG'CCT Cuts at: 1517 2939 1517 Size: 1422 4258 StyI C'CwwG_G Cuts at: 513 1065 2004 2847 3119 513 Size: 552 939 843 272 3074 Fragments arranged by size: 3074 939 843 552 272 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3707 3724 3877 4062 4507 325 Size: 1255 2127 17 153 185 445 1498 Fragments arranged by size: 2127 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2013 2967 3926 Size: 274 80 33 51 1443 36 954 959 Cuts at: 3926 5638 96 Size: 1712 138 Fragments arranged by size: 1712 1443 959 954 274 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5640 5672 5640 Size: 32 5648 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5680 XbaI T'CTAG_A Cuts at: 3016 3016 Size: 5680 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5680 XmaI C'CCGG_G Cuts at: 1870 3005 1870 Size: 1135 4545 XmnI GAAnn'nnTTC Cuts at: 3809 3809 Size: 5680 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3488 276 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI XcmI