Restriction map for
pFN21AB9811
(Linear) (Six-base) MAPSORT of: KIBB9811.seq Check: 5110 from: 1 to: 744
KIBB9811.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 744
Size: 382 362
AgeI A'CCGG_T
Cuts at: 0 406 744
Size: 406 338
AlwNI CAG_nnn'CTG
Cuts at: 0 666 744
Size: 666 78
ApaI G_GGCC'C
Cuts at: 0 236 744
Size: 236 508
AvaI C'yCGr_G
Cuts at: 0 235 559 744
Size: 235 324 185
Fragments arranged by size:
324 235 185
BanI G'GyrC_C
Cuts at: 0 82 744
Size: 82 662
BanII G_rGCy'C
Cuts at: 0 107 220 236 275 737 744
Size: 107 113 16 39 462 7
Fragments arranged by size:
462 113 107 39 16 7
BbeI G_GCGC'C
Cuts at: 0 86 744
Size: 86 658
BbsI GAAGACnn'nnnn_
Cuts at: 0 345 525 744
Size: 345 180 219
Fragments arranged by size:
345 219 180
BbvCI CC'TCA_GC
Cuts at: 0 622 744
Size: 622 122
BglI GCCn_nnn'nGGC
Cuts at: 0 34 400 744
Size: 34 366 344
Fragments arranged by size:
366 344 34
Bme1580I G_kGCm'C
Cuts at: 0 236 744
Size: 236 508
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 123 234 588 744
Size: 123 111 354 156
Fragments arranged by size:
354 156 123 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 259 744
Size: 195 64 485
Fragments arranged by size:
485 195 64
Bpu10I CC'TnA_GC
Cuts at: 0 622 744
Size: 622 122
BsaHI Gr'CG_yC
Cuts at: 0 83 338 744
Size: 83 255 406
Fragments arranged by size:
406 255 83
BsaWI w'CCGG_w
Cuts at: 0 406 744
Size: 406 338
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 226 238 577 744
Size: 115 111 12 339 167
Fragments arranged by size:
339 167 115 111 12
BseYI C'CCAG_C
Cuts at: 0 297 744
Size: 297 447
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 744
Size: 598 146
BsiEI CG_ry'CG
Cuts at: 0 695 744
Size: 695 49
BsiHKAI G_wGCw'C
Cuts at: 0 107 275 744
Size: 107 168 469
Fragments arranged by size:
469 168 107
Bsp1286I G_dGCh'C
Cuts at: 0 107 220 236 275 737 744
Size: 107 113 16 39 462 7
Fragments arranged by size:
462 113 107 39 16 7
BspHI T'CATG_A
Cuts at: 0 74 744
Size: 74 670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 744
Size: 134 610
BsrFI r'CCGG_y
Cuts at: 0 406 744
Size: 406 338
BstAPI GCAn_nnn'nTGC
Cuts at: 0 666 744
Size: 666 78
BstXI CCAn_nnnn'nTGG
Cuts at: 0 507 576 744
Size: 507 69 168
Fragments arranged by size:
507 168 69
BtgI C'CryG_G
Cuts at: 0 86 461 602 744
Size: 86 375 141 142
Fragments arranged by size:
375 142 141 86
EaeI y'GGCC_r
Cuts at: 0 159 392 401 578 692 744
Size: 159 233 9 177 114 52
Fragments arranged by size:
233 177 159 114 52 9
EagI C'GGCC_G
Cuts at: 0 692 744
Size: 692 52
EarI CTCTTCn'nnn_
Cuts at: 0 110 704 744
Size: 110 594 40
Fragments arranged by size:
594 110 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 493 744
Size: 493 251
EcoICRI GAG'CTC
Cuts at: 0 105 273 744
Size: 105 168 471
Fragments arranged by size:
471 168 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 259 382 744
Size: 195 64 123 362
Fragments arranged by size:
362 195 123 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 322 394 625 744
Size: 322 72 231 119
Fragments arranged by size:
322 231 119 72
EcoO109I rG'GnC_Cy
Cuts at: 0 232 744
Size: 232 512
HaeII r_GCGC'y
Cuts at: 0 86 744
Size: 86 658
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 744
Size: 29 715
KasI G'GCGC_C
Cuts at: 0 82 744
Size: 82 662
MscI TGG'CCA
Cuts at: 0 403 580 744
Size: 403 177 164
Fragments arranged by size:
403 177 164
MspA1I CmG'CkG
Cuts at: 0 663 744
Size: 663 81
NarI GG'CG_CC
Cuts at: 0 83 744
Size: 83 661
NcoI C'CATG_G
Cuts at: 0 461 602 744
Size: 461 141 142
Fragments arranged by size:
461 142 141
PspOMI G'GGCC_C
Cuts at: 0 232 744
Size: 232 512
PvuII CAG'CTG
Cuts at: 0 663 744
Size: 663 81
SacI G_AGCT'C
Cuts at: 0 107 275 744
Size: 107 168 469
Fragments arranged by size:
469 168 107
SapI GCTCTTCn'nnn_
Cuts at: 0 110 744
Size: 110 634
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 400 744
Size: 400 344
SfoI GGC'GCC
Cuts at: 0 84 744
Size: 84 660
SgrAI Cr'CCGG_yG
Cuts at: 0 406 744
Size: 406 338
StyI C'CwwG_G
Cuts at: 0 461 602 744
Size: 461 141 142
Fragments arranged by size:
461 142 141
Enzymes that do cut:
AcuI AgeI AlwNI ApaI AvaI BanI BanII BbeI
BbsI BbvCI BglI Bme1580I BplI BpmI Bpu10I BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrFI BstAPI BstXI BtgI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
MscI MspA1I NarI NcoI PspOMI PvuII SacI SapI
SfiI SfoI SgrAI StyI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BpuEI BsaI BsaAI
BsaBI BsaXI BsiWI BsmI BsmBI BspEI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MslI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfcI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI