(Circular) (Six-base) MAPSORT of: pFN21AB9805.seq Check: 7683 from: 1 to: 5380 pFN21AB9805 5380 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3190 278 Size: 53 83 186 936 1654 2468 Fragments arranged by size: 2468 1654 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2723 1937 Size: 786 4594 Acc65I G'GTAC_C Cuts at: 2701 2701 Size: 5380 AclI AA'CG_TT Cuts at: 1563 3507 3880 1563 Size: 1944 373 3063 Fragments arranged by size: 3063 1944 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3443 4827 5095 1632 Size: 1811 1384 268 1917 Fragments arranged by size: 1917 1811 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5183 AflIII A'CryG_T Cuts at: 1203 2034 4279 5256 1203 Size: 831 2245 977 1327 Fragments arranged by size: 2245 1327 977 831 AgeI A'CCGG_T Cuts at: 4211 4211 Size: 5380 AhdI GACnn_n'nnGTC Cuts at: 4109 4109 Size: 5380 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5380 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5380 AlwNI CAG_nnn'CTG Cuts at: 2476 2524 3123 4695 2476 Size: 48 599 1572 3161 Fragments arranged by size: 3161 1572 599 48 ApaI G_GGCC'C Cuts at: 2138 2610 2138 Size: 472 4908 ApaLI G'TGCA_C Cuts at: 3437 4593 3437 Size: 1156 4224 ApoI r'AATT_y Cuts at: 1493 2689 2915 3067 5176 1493 Size: 1196 226 152 2109 1697 Fragments arranged by size: 2109 1697 1196 226 152 AseI AT'TA_AT Cuts at: 160 1032 3934 160 Size: 872 2902 1606 Fragments arranged by size: 2902 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5380 AvaI C'yCGr_G Cuts at: 1870 1943 2137 2705 1870 Size: 73 194 568 4545 Fragments arranged by size: 4545 568 194 73 BamHI G'GATC_C Cuts at: 2710 3077 2710 Size: 367 5013 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2158 2490 2594 2701 4156 Size: 325 217 627 371 332 104 107 1455 Cuts at: 4156 618 Size: 1842 Fragments arranged by size: 1842 1455 627 371 332 325 217 107 104 BanII G_rGCy'C Cuts at: 1391 2016 2138 2610 2699 1391 Size: 625 122 472 89 4072 Fragments arranged by size: 4072 625 472 122 89 BbeI G_GCGC'C Cuts at: 2162 2494 2598 2162 Size: 332 104 4944 Fragments arranged by size: 4944 332 104 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4409 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3571 3605 1903 Size: 34 1634 34 3678 Fragments arranged by size: 3678 1634 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3272 4482 3272 Size: 1210 4170 BclI T'GATC_A Cuts at: 1548 1548 Size: 5380 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2090 2142 3991 Size: 107 122 71 631 605 418 52 1849 Cuts at: 3991 136 Size: 1525 Fragments arranged by size: 1849 1525 631 605 418 122 107 71 52 BglII A'GATC_T Cuts at: 5375 5375 Size: 5380 BlpI GC'TnA_GC Cuts at: 2312 2797 2312 Size: 485 4895 Bme1580I G_kGCm'C Cuts at: 1790 2138 2582 2610 3441 4597 1790 Size: 348 444 28 831 1156 2573 Fragments arranged by size: 2573 1156 831 444 348 28 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5380 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3111 4069 442 Size: 416 53 83 399 24 1694 958 1753 Fragments arranged by size: 1753 1694 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5380 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4040 1239 Size: 2801 2579 Bpu10I CC'TnA_GC Cuts at: 2181 2181 Size: 5380 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2000 3505 4370 4668 2000 Size: 1505 865 298 2712 Fragments arranged by size: 2712 1505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2084 2307 4043 882 Size: 617 585 223 1736 2219 Fragments arranged by size: 2219 1736 617 585 223 BsaAI yAC'GTr Cuts at: 493 1204 3171 493 Size: 711 1967 2702 Fragments arranged by size: 2702 1967 711 BsaBI GATnn'nnATC Cuts at: 5374 5374 Size: 5380 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2159 2226 2491 2555 Size: 53 83 186 936 626 67 265 64 Cuts at: 2555 2595 2642 3187 3569 4204 275 Size: 40 47 545 382 635 1451 Fragments arranged by size: 1451 936 635 626 545 382 265 186 83 67 64 53 47 40 BsaWI w'CCGG_w Cuts at: 3813 4211 4485 4632 5043 3813 Size: 398 274 147 411 4150 Fragments arranged by size: 4150 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5155 Fragments arranged by size: 5155 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5380 BseYI C'CCAG_C Cuts at: 2176 4583 5079 2176 Size: 2407 496 2477 Fragments arranged by size: 2477 2407 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2148 1163 Size: 573 412 4395 Fragments arranged by size: 4395 573 412 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2288 2830 3591 4619 1540 Size: 275 186 287 542 761 1028 2301 Fragments arranged by size: 2301 1028 761 542 287 275 186 BsiHKAI G_wGCw'C Cuts at: 2016 3441 3526 4597 2016 Size: 1425 85 1071 2799 Fragments arranged by size: 2799 1425 1071 85 BsmI GAATG_Cn' Cuts at: 2891 2984 2891 Size: 93 5287 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2138 2582 2610 2699 3441 3526 Size: 399 226 122 444 28 89 742 85 Cuts at: 3526 4597 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 444 399 226 122 89 85 28 BspEI T'CCGG_A Cuts at: 5043 5043 Size: 5380 BspHI T'CATG_A Cuts at: 3269 4999 3269 Size: 1730 3650 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2735 844 Size: 1891 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2211 2332 3267 1394 Size: 429 388 121 935 3507 Fragments arranged by size: 3507 935 429 388 121 BsrDI GCAATG_nn' Cuts at: 65 1248 3875 4049 65 Size: 1183 2627 174 1396 Fragments arranged by size: 2627 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2677 4024 4211 1700 Size: 110 867 1347 187 2869 Fragments arranged by size: 2869 1347 867 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5380 BssHII G'CGCG_C Cuts at: 1925 2324 1925 Size: 399 4981 BssSI C'ACGA_G Cuts at: 2669 3440 4452 2669 Size: 771 1012 3597 Fragments arranged by size: 3597 1012 771 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5380 BstBI TT'CG_AA Cuts at: 4184 4184 Size: 5380 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5380 BstYI r'GATC_y Cuts at: 1972 2710 3077 3462 3479 4931 5017 5029 5375 Size: 738 367 385 17 1452 86 12 346 Cuts at: 5375 1972 Size: 1977 Fragments arranged by size: 1977 1452 738 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2053 2116 2154 513 Size: 552 939 49 63 38 3739 Fragments arranged by size: 3739 939 552 63 49 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4641 Fragments arranged by size: 4641 683 56 BtsI GCAGTG_nn' Cuts at: 2896 3690 3710 2896 Size: 794 20 4566 Fragments arranged by size: 4566 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5380 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5345 DraI TTT'AAA Cuts at: 2684 2684 Size: 5380 DraIII CAC_nnn'GTG Cuts at: 2577 2577 Size: 5380 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4387 809 Size: 507 3071 1802 Fragments arranged by size: 3071 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2285 2526 Size: 54 1425 50 275 18 177 278 241 Cuts at: 2526 2563 2827 3716 5345 8 Size: 37 264 889 1629 43 Fragments arranged by size: 1629 1425 889 278 275 264 241 177 54 50 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 2285 2827 1537 Size: 275 473 542 4090 Fragments arranged by size: 4090 542 473 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3310 1353 Size: 1957 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2204 2285 3967 4341 4487 387 Size: 1064 138 615 81 1682 374 146 1280 Fragments arranged by size: 1682 1280 1064 615 374 146 138 81 EcoICRI GAG'CTC Cuts at: 2014 2014 Size: 5380 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3443 4040 4827 5095 1239 Size: 393 1811 597 787 268 1524 Fragments arranged by size: 1811 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2462 2510 2462 Size: 48 5332 EcoO109I rG'GnC_Cy Cuts at: 2473 2521 2606 2607 2473 Size: 48 85 1 5246 Fragments arranged by size: 5246 85 48 1 EcoRI G'AATT_C Cuts at: 2689 3067 2689 Size: 378 5002 FspI TGC'GCA Cuts at: 2411 3886 2411 Size: 1475 3905 HaeII r_GCGC'y Cuts at: 1678 2162 2494 2598 4527 1678 Size: 484 332 104 1929 2531 Fragments arranged by size: 2531 1929 484 332 104 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 2285 2317 2367 Size: 32 135 32 431 32 250 32 50 Cuts at: 2367 2399 4021 4053 4095 4127 1373 Size: 32 1622 32 42 32 2626 Fragments arranged by size: 2626 1622 431 250 135 50 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2724 2970 669 Size: 988 281 786 246 3079 Fragments arranged by size: 3079 988 786 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5380 HpaI GTT'AAC Cuts at: 2970 2970 Size: 5380 KasI G'GCGC_C Cuts at: 2158 2490 2594 2158 Size: 332 104 4944 Fragments arranged by size: 4944 332 104 KpnI G_GTAC'C Cuts at: 2705 2705 Size: 5380 MfeI C'AATT_G Cuts at: 2979 2979 Size: 5380 MluI A'CGCG_T Cuts at: 2034 5256 2034 Size: 3222 2158 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4494 4678 1305 Size: 3189 184 2007 Fragments arranged by size: 3189 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3558 Fragments arranged by size: 3558 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3338 3697 3856 518 Size: 648 2172 359 159 2042 Fragments arranged by size: 2172 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2118 2156 2199 2239 2299 2796 3473 4621 Size: 512 38 43 40 60 497 677 1148 Cuts at: 4621 4866 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 512 497 245 60 43 40 38 NarI GG'CG_CC Cuts at: 2159 2491 2595 2159 Size: 332 104 4944 Fragments arranged by size: 4944 332 104 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3889 Fragments arranged by size: 3889 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5380 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5380 NotI GC'GGCC_GC Cuts at: 2285 2827 2285 Size: 542 4838 NspI r_CATG'y Cuts at: 1132 1229 2344 2738 4283 1132 Size: 97 1115 394 1545 2229 Fragments arranged by size: 2229 1545 1115 394 97 PasI CC'CwG_GG Cuts at: 1348 2602 1348 Size: 1254 4126 PciI A'CATG_T Cuts at: 4279 4279 Size: 5380 PflMI CCAn_nnn'nTGG Cuts at: 1108 2524 1108 Size: 1416 3964 PfoI T'CCnGG_A Cuts at: 2636 2636 Size: 5380 PmeI GTTT'AAAC Cuts at: 2684 2684 Size: 5380 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3449 3481 4962 4994 3449 Size: 32 1481 32 3835 Fragments arranged by size: 3835 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5380 PsiI TTA'TAA Cuts at: 2950 2950 Size: 5380 PspOMI G'GGCC_C Cuts at: 2134 2606 2134 Size: 472 4908 PstI C_TGCA'G Cuts at: 830 2732 830 Size: 1902 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5380 SacI G_AGCT'C Cuts at: 2016 2016 Size: 5380 SacII CC_GC'GG Cuts at: 2119 2157 2119 Size: 38 5342 SalI G'TCGA_C Cuts at: 1936 2722 1936 Size: 786 4594 SbfI CC_TGCA'GG Cuts at: 2732 2732 Size: 5380 ScaI AGT'ACT Cuts at: 3628 5340 3628 Size: 1712 3668 SfcI C'TryA_G Cuts at: 826 1045 2728 3863 4544 4735 826 Size: 219 1683 1135 681 191 1471 Fragments arranged by size: 1683 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2160 2492 2596 2160 Size: 332 104 4944 Fragments arranged by size: 4944 332 104 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5380 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5380 SmaI CCC'GGG Cuts at: 1872 2139 2707 1872 Size: 267 568 4545 Fragments arranged by size: 4545 568 267 SmlI C'TyrA_G Cuts at: 820 1017 1943 2015 3484 4385 4647 820 Size: 197 926 72 1469 901 262 1553 Fragments arranged by size: 1553 1469 926 901 262 197 72 SnaBI TAC'GTA Cuts at: 493 3171 493 Size: 2678 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5380 SphI G_CATG'C Cuts at: 1132 2344 2738 1132 Size: 1212 394 3774 Fragments arranged by size: 3774 1212 394 SrfI GCCC'GGGC Cuts at: 2139 2139 Size: 5380 SspI AAT'ATT Cuts at: 5 52 3304 5 Size: 47 3252 2081 Fragments arranged by size: 3252 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5380 StyI C'CwwG_G Cuts at: 513 1065 2004 2819 513 Size: 552 939 815 3074 Fragments arranged by size: 3074 939 815 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3407 3424 3577 3762 4207 325 Size: 1255 1827 17 153 185 445 1498 Fragments arranged by size: 1827 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3626 5338 96 Size: 274 80 33 51 1443 1649 1712 138 Fragments arranged by size: 1712 1649 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5340 5372 5340 Size: 32 5348 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5380 XbaI T'CTAG_A Cuts at: 2716 2716 Size: 5380 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5380 XmaI C'CCGG_G Cuts at: 1870 2137 2705 1870 Size: 267 568 4545 Fragments arranged by size: 4545 568 267 XmnI GAAnn'nnTTC Cuts at: 3509 3509 Size: 5380 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3188 276 Size: 53 83 186 936 1654 2468 Fragments arranged by size: 2468 1654 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SwaI XcmI