Restriction map for pFN21AB9791
 (Circular) (Six-base) MAPSORT of: pFN21AB9791.seq  Check: 2596  from: 1  to: 5335



pFN21AB9791  5335 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2207    2453    2207 
   Size:        246    5089
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3145     278 
   Size:         53      83     186     936    1609    2468

  Fragments arranged by size: 

                2468    1609     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2678    1937 
   Size:        741    4594
 
Acc65I G'GTAC_C

Cuts at:   2656    2656 
   Size:       5335
 
AclI AA'CG_TT

Cuts at:   1563    3462    3835    1563 
   Size:       1899     373    3063

  Fragments arranged by size: 

                3063    1899     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3398    4782    5050    1632 
   Size:       1766    1384     268    1917

  Fragments arranged by size: 

                1917    1766    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5138
 
AflIII A'CryG_T

Cuts at:   1203    4234    5211    1203 
   Size:       3031     977    1327

  Fragments arranged by size: 

                3031    1327     977
 
AgeI A'CCGG_T

Cuts at:   4166    4166 
   Size:       5335
 
AhdI GACnn_n'nnGTC

Cuts at:   4064    4064 
   Size:       5335
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5335
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5335
 
AlwNI CAG_nnn'CTG

Cuts at:   2310    3078    4650    2310 
   Size:        768    1572    2995

  Fragments arranged by size: 

                2995    1572     768
 
ApaI G_GGCC'C

Cuts at:   2172    2172 
   Size:       5335
 
ApaLI G'TGCA_C

Cuts at:   3392    4548    3392 
   Size:       1156    4179
 
ApoI r'AATT_y

Cuts at:   1493    2644    2870    3022    5131    1493 
   Size:       1151     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1151     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3889     160 
   Size:        872    2857    1606

  Fragments arranged by size: 

                2857    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5335
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2136    2455    2660    1870 
   Size:         73     193     319     205    4545

  Fragments arranged by size: 

                4545     319     205     193      73
 
BamHI G'GATC_C

Cuts at:   2665    3032    2665 
   Size:        367    4968
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2156    2219    2374    2656    4111 
   Size:        325     217     627     369      63     155     282    1455

Cuts at:   4111     618 
   Size:       1842

  Fragments arranged by size: 

                1842    1455     627     369     325     282     217     155
                  63
 
BanII G_rGCy'C

Cuts at:   1391    2172    2654    1391 
   Size:        781     482    4072

  Fragments arranged by size: 

                4072     781     482
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4364
 
BbvCI CC'TCA_GC

Cuts at:   2442    2615    2442 
   Size:        173    5162
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    2360    2394    3526    3560    1903 
   Size:         34      50      34     339      34    1132      34    3678

  Fragments arranged by size: 

                3678    1132     339      50      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   3227    4437    3227 
   Size:       1210    4125
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5335
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2568    2625    3946 
   Size:        107     122      71     631     605     896      57    1321

Cuts at:   3946     136 
   Size:       1525

  Fragments arranged by size: 

                1525    1321     896     631     605     122     107      71
                  57
 
BglII A'GATC_T

Cuts at:   5330    5330 
   Size:       5335
 
BlpI GC'TnA_GC

Cuts at:   2752    2752 
   Size:       5335
 
Bme1580I G_kGCm'C

Cuts at:   1790    2161    2172    2224    3396    4552    1790 
   Size:        371      11      52    1172    1156    2573

  Fragments arranged by size: 

                2573    1172    1156     371      52      11
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5335
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2052    2509    3066 
   Size:        416      53      83     399      24     635     457     557

Cuts at:   3066    4024     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753     958     635     557     457     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5335
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2210    3995    1239 
   Size:        971    1785    2579

  Fragments arranged by size: 

                2579    1785     971
 
Bpu10I CC'TnA_GC

Cuts at:   2442    2615    2442 
   Size:        173    5162
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3460    4325    4623    3460 
   Size:        865     298    4172

  Fragments arranged by size: 

                4172     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3998     882 
   Size:        617    2499    2219

  Fragments arranged by size: 

                2499    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3126     493 
   Size:        711    1922    2702

  Fragments arranged by size: 

                2702    1922     711
 
BsaBI GATnn'nnATC

Cuts at:   5329    5329 
   Size:       5335
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3142    3524    4159     275 
   Size:         53      83     186     936    1609     382     635    1451

  Fragments arranged by size: 

                1609    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3768    4166    4440    4587    4998    3768 
   Size:        398     274     147     411    4105

  Fragments arranged by size: 

                4105     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2114    2144    1178 
   Size:         30     165      30     711      30    4369

  Fragments arranged by size: 

                4369     711     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2030    2042    2603    1186 
   Size:        844      12     561    3918

  Fragments arranged by size: 

                3918     844     561      12
 
BseYI C'CCAG_C

Cuts at:   2165    2195    2310    4538    5034    2165 
   Size:         30     115    2228     496    2466

  Fragments arranged by size: 

                2466    2228     496     115      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2244    2590    1163 
   Size:        573     508     346    3908

  Fragments arranged by size: 

                3908     573     508     346
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2785    3546    4574    1540 
   Size:        275     186     784     761    1028    2301

  Fragments arranged by size: 

                2301    1028     784     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3396    3481    4552    3396 
   Size:         85    1071    4179

  Fragments arranged by size: 

                4179    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2846    2939    2846 
   Size:         93    5242
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2161    2172    2224    2654    3396    3481    4552 
   Size:        399     371      11      52     430     742      85    1071

Cuts at:   4552    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     430     399     371      85      52
                  11
 
BspEI T'CCGG_A

Cuts at:   4998    4998 
   Size:       5335
 
BspHI T'CATG_A

Cuts at:   3224    4954    3224 
   Size:       1730    3605
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2207    2453    2690     844 
   Size:       1363     246     237    3489

  Fragments arranged by size: 

                3489    1363     246     237
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3222    1394 
   Size:        429    1399    3507

  Fragments arranged by size: 

                3507    1399     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2093    3830    4004      65 
   Size:       1183     845    1737     174    1396

  Fragments arranged by size: 

                1737    1396    1183     845     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2238    3979    4166    1700 
   Size:        110     428    1741     187    2869

  Fragments arranged by size: 

                2869    1741     428     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5335
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5335
 
BssSI C'ACGA_G

Cuts at:   3395    4407    3395 
   Size:       1012    4323
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5335
 
BstBI TT'CG_AA

Cuts at:   2584    4139    2584 
   Size:       1555    3780
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5335
 
BstYI r'GATC_y

Cuts at:   1972    2665    3032    3417    3434    4886    4972    4984    5330 
   Size:        693     367     385      17    1452      86      12     346

Cuts at:   5330    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     693     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4783
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2310    2590     487 
   Size:        683      56    1084     280    3232

  Fragments arranged by size: 

                3232    1084     683     280      56
 
BtsI GCAGTG_nn'

Cuts at:   2592    2851    3645    3665    2592 
   Size:        259     794      20    4262

  Fragments arranged by size: 

                4262     794     259      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5335
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5300
 
DraI TTT'AAA

Cuts at:   2639    2639 
   Size:       5335
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4342     809 
   Size:        507    3026    1802

  Fragments arranged by size: 

                3026    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2226    2626    2782 
   Size:         54    1425      50     275      18     396     400     156

Cuts at:   2782    3671    5300       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     400     396     275     156      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2782    1537 
   Size:        275     970    4090

  Fragments arranged by size: 

                4090     970     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2186    3265    1353 
   Size:        833    1079    3423

  Fragments arranged by size: 

                3423    1079     833
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2390    3922    4296    4442     387 
   Size:       1064     138     801    1532     374     146    1280

  Fragments arranged by size: 

                1532    1280    1064     801     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2210    3398    3995    4782    5050    1239 
   Size:        393     578    1188     597     787     268    1524

  Fragments arranged by size: 

                1524    1188     787     597     578     393     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2189    2189 
   Size:       5335
 
EcoO109I rG'GnC_Cy

Cuts at:   2122    2169    2199    2122 
   Size:         47      30    5258

  Fragments arranged by size: 

                5258      47      30
 
EcoRI G'AATT_C

Cuts at:   2644    3022    2644 
   Size:        378    4957
 
FspI TGC'GCA

Cuts at:   3841    3841 
   Size:       5335
 
HaeII r_GCGC'y

Cuts at:   1678    4482    1678 
   Size:       2804    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2114    2146    3976    4008    4050 
   Size:         32     135      32     542      32    1830      32      42

Cuts at:   4050    4082    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1830     542     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2679    2925     669 
   Size:        988     281     741     246    3079

  Fragments arranged by size: 

                3079     988     741     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5335
 
HpaI GTT'AAC

Cuts at:   2925    2925 
   Size:       5335
 
KpnI G_GTAC'C

Cuts at:   2660    2660 
   Size:       5335
 
MfeI C'AATT_G

Cuts at:   2067    2934    2067 
   Size:        867    4468
 
MluI A'CGCG_T

Cuts at:   5211    5211 
   Size:       5335
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4449    4633    1305 
   Size:       3144     184    2007

  Fragments arranged by size: 

                3144    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3513

  Fragments arranged by size: 

                3513    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3293    3652    3811     518 
   Size:        648    2127     359     159    2042

  Fragments arranged by size: 

                2127    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2333    2384    2424    2549    2751    3428    4576    4821 
   Size:        727      51      40     125     202     677    1148     245

Cuts at:   4821    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     727     677     245     202     125      51
                  40
 
NaeI GCC'GGC

Cuts at:   2240    2240 
   Size:       5335
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4783
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5335
 
NgoMIV G'CCGG_C

Cuts at:   2238    2238 
   Size:       5335
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5335
 
NotI GC'GGCC_GC

Cuts at:   2782    2782 
   Size:       5335
 
NspI r_CATG'y

Cuts at:   1132    1229    2693    4238    1132 
   Size:         97    1464    1545    2229

  Fragments arranged by size: 

                2229    1545    1464      97
 
PasI CC'CwG_GG

Cuts at:   1348    2557    1348 
   Size:       1209    4126
 
PciI A'CATG_T

Cuts at:   4234    4234 
   Size:       5335
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5335
 
PmeI GTTT'AAAC

Cuts at:   2639    2639 
   Size:       5335
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3404    3436    4917    4949    3404 
   Size:         32    1481      32    3790

  Fragments arranged by size: 

                3790    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2122    2122 
   Size:       5335
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5335
 
PsiI TTA'TAA

Cuts at:   2905    2905 
   Size:       5335
 
PspOMI G'GGCC_C

Cuts at:   2168    2168 
   Size:       5335
 
PstI C_TGCA'G

Cuts at:    830    2383    2687     830 
   Size:       1553     304    3478

  Fragments arranged by size: 

                3478    1553     304
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5335
 
PvuII CAG'CTG

Cuts at:   2384    2384 
   Size:       5335
 
RsrII CG'GwC_CG

Cuts at:   2328    2328 
   Size:       5335
 
SalI G'TCGA_C

Cuts at:   1936    2677    1936 
   Size:        741    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2186    2186 
   Size:       5335
 
SbfI CC_TGCA'GG

Cuts at:   2687    2687 
   Size:       5335
 
ScaI AGT'ACT

Cuts at:   2408    3583    5295    2408 
   Size:       1175    1712    2448

  Fragments arranged by size: 

                2448    1712    1175
 
SfcI C'TryA_G

Cuts at:    826    1045    2379    2683    3818    4499    4690     826 
   Size:        219    1334     304    1135     681     191    1471

  Fragments arranged by size: 

                1471    1334    1135     681     304     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5335
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5335
 
SmaI CCC'GGG

Cuts at:   1872    2457    2662    1872 
   Size:        585     205    4545

  Fragments arranged by size: 

                4545     585     205
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3439    4340    4602     820 
   Size:        197     926    1496     901     262    1553

  Fragments arranged by size: 

                1553    1496     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3126     493 
   Size:       2633    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5335
 
SphI G_CATG'C

Cuts at:   1132    2693    1132 
   Size:       1561    3774
 
SspI AAT'ATT

Cuts at:      5      52    3259       5 
   Size:         47    3207    2081

  Fragments arranged by size: 

                3207    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5335
 
StyI C'CwwG_G

Cuts at:    513    1065    2774     513 
   Size:        552    1709    3074

  Fragments arranged by size: 

                3074    1709     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2302    3362    3379    3532    3717    4162     325 
   Size:       1255     722    1060      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1060     722     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2406    3581    5293 
   Size:        274      80      33      51    1443     429    1175    1712

Cuts at:   5293      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1175     429     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5295    5327    5295 
   Size:         32    5303
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5335
 
XbaI T'CTAG_A

Cuts at:   2671    2671 
   Size:       5335
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5335
 
XmaI C'CCGG_G

Cuts at:   1870    2455    2660    1870 
   Size:        585     205    4545

  Fragments arranged by size: 

                4545     585     205
 
XmnI GAAnn'nnTTC

Cuts at:   3464    3464 
   Size:       5335
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3143     276 
   Size:         53      83     186     936    1609    2468

  Fragments arranged by size: 

                2468    1609     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII    RsrII     SalI
     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BfrBI     BplI
    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoRV
     FalI     FseI    FspAI     KasI     NarI     NruI     NsiI     PacI
     PfoI     PmlI    PspXI     PsrI     SacI    SacII    SanDI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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