(Circular) (Six-base) MAPSORT of: pFN21AB9777.seq Check: 4147 from: 1 to: 7069 pFN21AB9777 7069 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4879 278 Size: 53 83 186 936 3343 2468 Fragments arranged by size: 3343 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4092 4412 1937 Size: 2155 320 4594 Fragments arranged by size: 4594 2155 320 Acc65I G'GTAC_C Cuts at: 4390 4390 Size: 7069 AclI AA'CG_TT Cuts at: 1563 5196 5569 1563 Size: 3633 373 3063 Fragments arranged by size: 3633 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2012 2481 2876 2933 3506 5132 6516 6784 Size: 380 469 395 57 573 1626 1384 268 Cuts at: 6784 1632 Size: 1917 Fragments arranged by size: 1917 1626 1384 573 469 395 380 268 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6872 AflIII A'CryG_T Cuts at: 1203 5968 6945 1203 Size: 4765 977 1327 Fragments arranged by size: 4765 1327 977 AgeI A'CCGG_T Cuts at: 5900 5900 Size: 7069 AhdI GACnn_n'nnGTC Cuts at: 5798 5798 Size: 7069 AleI CACnn'nnGTG Cuts at: 1166 2574 3283 1166 Size: 1408 709 4952 Fragments arranged by size: 4952 1408 709 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7069 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3728 3760 3728 Size: 32 7037 AlwNI CAG_nnn'CTG Cuts at: 2842 3331 4812 6384 2842 Size: 489 1481 1572 3527 Fragments arranged by size: 3527 1572 1481 489 ApaI G_GGCC'C Cuts at: 3306 3306 Size: 7069 ApaLI G'TGCA_C Cuts at: 5126 6282 5126 Size: 1156 5913 ApoI r'AATT_y Cuts at: 1493 2263 2311 3128 4378 4604 4756 6865 1493 Size: 770 48 817 1250 226 152 2109 1697 Fragments arranged by size: 2109 1697 1250 817 770 226 152 48 AseI AT'TA_AT Cuts at: 160 1032 2271 5623 160 Size: 872 1239 3352 1606 Fragments arranged by size: 3352 1606 1239 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7069 AvaI C'yCGr_G Cuts at: 1870 1943 2099 2810 3309 3978 4394 1870 Size: 73 156 711 499 669 416 4545 Fragments arranged by size: 4545 711 669 499 416 156 73 BamHI G'GATC_C Cuts at: 4007 4399 4766 4007 Size: 392 367 6310 Fragments arranged by size: 6310 392 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3034 4390 5845 618 Size: 325 217 627 1247 1356 1455 1842 Fragments arranged by size: 1842 1455 1356 1247 627 325 217 BanII G_rGCy'C Cuts at: 1391 2307 2934 3306 4388 1391 Size: 916 627 372 1082 4072 Fragments arranged by size: 4072 1082 916 627 372 BbeI G_GCGC'C Cuts at: 3038 3038 Size: 7069 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2313 2882 3281 3300 3494 928 Size: 971 414 569 399 19 194 4503 Fragments arranged by size: 4503 971 569 414 399 194 19 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5260 5294 1903 Size: 34 3323 34 3678 Fragments arranged by size: 3678 3323 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4961 6171 4961 Size: 1210 5859 BclI T'GATC_A Cuts at: 1548 2833 1548 Size: 1285 5784 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5680 136 Size: 107 122 71 631 605 4008 1525 Fragments arranged by size: 4008 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7064 7064 Size: 7069 BlpI GC'TnA_GC Cuts at: 2996 4486 2996 Size: 1490 5579 Bme1580I G_kGCm'C Cuts at: 1790 3306 5130 6286 1790 Size: 1516 1824 1156 2573 Fragments arranged by size: 2573 1824 1516 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7069 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2746 3319 3534 Size: 416 53 83 399 24 1329 573 215 Cuts at: 3534 4800 5758 442 Size: 1266 958 1753 Fragments arranged by size: 1753 1329 1266 958 573 416 399 215 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7069 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2232 2714 2882 3353 3567 3746 4384 5729 Size: 993 482 168 471 214 179 638 1345 Cuts at: 5729 1239 Size: 2579 Fragments arranged by size: 2579 1345 993 638 482 471 214 179 168 Bpu10I CC'TnA_GC Cuts at: 2485 2485 Size: 7069 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2291 5194 6059 6357 2291 Size: 2903 865 298 3003 Fragments arranged by size: 3003 2903 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2954 4029 5732 882 Size: 617 1455 1075 1703 2219 Fragments arranged by size: 2219 1703 1455 1075 617 BsaAI yAC'GTr Cuts at: 493 1204 4860 493 Size: 711 3656 2702 Fragments arranged by size: 3656 2702 711 BsaBI GATnn'nnATC Cuts at: 7063 7063 Size: 7069 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3035 4876 5258 5893 Size: 53 83 186 936 1502 1841 382 635 Cuts at: 5893 275 Size: 1451 Fragments arranged by size: 1841 1502 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2742 4325 5502 5900 6174 6321 6732 2742 Size: 1583 1177 398 274 147 411 3079 Fragments arranged by size: 3079 1583 1177 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2205 2235 3560 3590 3728 Size: 30 165 30 802 30 1325 30 138 Cuts at: 3728 3758 1178 Size: 30 4489 Fragments arranged by size: 4489 1325 802 165 138 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2435 3012 3833 4259 1186 Size: 1249 577 821 426 3996 Fragments arranged by size: 3996 1249 821 577 426 BseYI C'CCAG_C Cuts at: 2944 3038 3739 6272 6768 2944 Size: 94 701 2533 496 3245 Fragments arranged by size: 3245 2533 701 496 94 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2804 1163 Size: 573 1068 5428 Fragments arranged by size: 5428 1068 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4519 5280 6308 1540 Size: 275 186 2518 761 1028 2301 Fragments arranged by size: 2518 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2307 2934 3290 3581 3627 5130 5215 6286 2307 Size: 627 356 291 46 1503 85 1071 3090 Fragments arranged by size: 3090 1503 1071 627 356 291 85 46 BsmI GAATG_Cn' Cuts at: 2955 3612 4580 4673 2955 Size: 657 968 93 5351 Fragments arranged by size: 5351 968 657 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2307 2934 3290 3306 3581 3627 4388 Size: 399 517 627 356 16 275 46 761 Cuts at: 4388 5130 5215 6286 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 761 742 627 517 399 356 275 85 46 16 BspEI T'CCGG_A Cuts at: 4325 6732 4325 Size: 2407 4662 BspHI T'CATG_A Cuts at: 4958 6688 4958 Size: 1730 5339 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2644 3337 4424 844 Size: 1800 693 1087 3489 Fragments arranged by size: 3489 1800 1087 693 BsrBI CCG'CTC Cuts at: 1394 1823 4956 1394 Size: 429 3133 3507 Fragments arranged by size: 3507 3133 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2305 3841 5564 5738 65 Size: 1183 1057 1536 1723 174 1396 Fragments arranged by size: 1723 1536 1396 1183 1057 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2632 2778 5713 5900 1700 Size: 110 822 146 2935 187 2869 Fragments arranged by size: 2935 2869 822 187 146 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7069 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7069 BssSI C'ACGA_G Cuts at: 3620 5129 6141 3620 Size: 1509 1012 4548 Fragments arranged by size: 4548 1509 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7069 BstBI TT'CG_AA Cuts at: 5873 5873 Size: 7069 BstEII G'GTnAC_C Cuts at: 1184 2579 1184 Size: 1395 5674 BstXI CCAn_nnnn'nTGG Cuts at: 2860 2860 Size: 7069 BstYI r'GATC_y Cuts at: 1972 3193 3481 4007 4322 4399 4766 5151 5168 Size: 1221 288 526 315 77 367 385 17 Cuts at: 5168 6620 6706 6718 7064 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1221 526 385 367 346 315 288 86 77 17 12 Bsu36I CC'TnA_GG Cuts at: 2112 2409 3435 2112 Size: 297 1026 5746 Fragments arranged by size: 5746 1026 297 BtgI C'CryG_G Cuts at: 513 1065 3030 513 Size: 552 1965 4552 Fragments arranged by size: 4552 1965 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6330 Fragments arranged by size: 6330 683 56 BtsI GCAGTG_nn' Cuts at: 2581 2614 3912 4585 5379 5399 2581 Size: 33 1298 673 794 20 4251 Fragments arranged by size: 4251 1298 794 673 33 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7069 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7034 DraI TTT'AAA Cuts at: 4165 4373 4165 Size: 208 6861 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2743 6076 809 Size: 507 1427 3333 1802 Fragments arranged by size: 3333 1802 1427 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3694 4069 4516 Size: 54 1425 50 275 18 1864 375 447 Cuts at: 4516 5405 7034 8 Size: 889 1629 43 Fragments arranged by size: 1864 1629 1425 889 447 375 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4516 1537 Size: 275 2704 4090 Fragments arranged by size: 4090 2704 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2030 2376 2667 2909 3444 4236 4999 1353 Size: 677 346 291 242 535 792 763 3423 Fragments arranged by size: 3423 792 763 677 535 346 291 242 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3874 5656 6030 6176 387 Size: 1064 138 2285 1782 374 146 1280 Fragments arranged by size: 2285 1782 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2305 2932 2305 Size: 627 6442 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2012 2232 2481 2714 2876 2882 2933 Size: 393 380 220 249 233 162 6 51 Cuts at: 2933 3353 3506 3567 3746 4384 5132 5729 6516 Size: 420 153 61 179 638 748 597 787 Cuts at: 6516 6784 1239 Size: 268 1524 Fragments arranged by size: 1524 787 748 638 597 420 393 380 268 249 233 220 179 162 153 61 51 6 EcoNI CCTnn'n_nnAGG Cuts at: 2337 2337 Size: 7069 EcoO109I rG'GnC_Cy Cuts at: 2085 2413 2794 3302 2085 Size: 328 381 508 5852 Fragments arranged by size: 5852 508 381 328 EcoRI G'AATT_C Cuts at: 2311 4378 4756 2311 Size: 2067 378 4624 Fragments arranged by size: 4624 2067 378 EcoRV GAT'ATC Cuts at: 3871 3871 Size: 7069 FspI TGC'GCA Cuts at: 5575 5575 Size: 7069 HaeII r_GCGC'y Cuts at: 1678 2533 3038 3725 6216 1678 Size: 855 505 687 2491 2531 Fragments arranged by size: 2531 2491 855 687 505 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3565 3597 4256 4288 4307 Size: 32 135 32 1993 32 659 32 19 Cuts at: 4307 4339 5710 5742 5784 5816 1373 Size: 32 1371 32 42 32 2626 Fragments arranged by size: 2626 1993 1371 659 135 42 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 4093 4413 4659 669 Size: 988 281 2155 320 246 3079 Fragments arranged by size: 3079 2155 988 320 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7069 HpaI GTT'AAC Cuts at: 4659 4659 Size: 7069 KasI G'GCGC_C Cuts at: 3034 3034 Size: 7069 KpnI G_GTAC'C Cuts at: 4394 4394 Size: 7069 MfeI C'AATT_G Cuts at: 4668 4668 Size: 7069 MluI A'CGCG_T Cuts at: 6945 6945 Size: 7069 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2117 2336 3459 6183 6367 1305 Size: 812 219 1123 2724 184 2007 Fragments arranged by size: 2724 2007 1123 812 219 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3696 4071 10 Size: 54 1425 343 1864 375 3008 Fragments arranged by size: 3008 1864 1425 375 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2160 2574 3283 5027 5386 5545 518 Size: 648 994 414 709 1744 359 159 2042 Fragments arranged by size: 2042 1744 994 709 648 414 359 159 MspA1I CmG'CkG Cuts at: 1606 2860 2944 3076 4024 4485 5162 6310 6555 Size: 1254 84 132 948 461 677 1148 245 Cuts at: 6555 1606 Size: 2120 Fragments arranged by size: 2120 1254 1148 948 677 461 245 132 84 NaeI GCC'GGC Cuts at: 2634 2780 2634 Size: 146 6923 NarI GG'CG_CC Cuts at: 3035 3035 Size: 7069 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6517 NdeI CA'TA_TG Cuts at: 387 387 Size: 7069 NgoMIV G'CCGG_C Cuts at: 2632 2778 2632 Size: 146 6923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7069 NotI GC'GGCC_GC Cuts at: 4516 4516 Size: 7069 NspI r_CATG'y Cuts at: 1132 1229 4427 5972 1132 Size: 97 3198 1545 2229 Fragments arranged by size: 3198 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2252 1348 Size: 904 6165 PciI A'CATG_T Cuts at: 5968 5968 Size: 7069 PflMI CCAn_nnn'nTGG Cuts at: 1108 3331 3639 1108 Size: 2223 308 4538 Fragments arranged by size: 4538 2223 308 PmeI GTTT'AAAC Cuts at: 4373 4373 Size: 7069 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5138 5170 6651 6683 3728 Size: 32 1378 32 1481 32 4114 Fragments arranged by size: 4114 1481 1378 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2085 2413 2794 2085 Size: 328 381 6360 Fragments arranged by size: 6360 381 328 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7069 PsiI TTA'TAA Cuts at: 4639 4639 Size: 7069 PspOMI G'GGCC_C Cuts at: 3302 3302 Size: 7069 PspXI vC'TCGA_Gb Cuts at: 2810 2810 Size: 7069 PstI C_TGCA'G Cuts at: 830 2173 2655 3348 3717 4421 830 Size: 1343 482 693 369 704 3478 Fragments arranged by size: 3478 1343 704 693 482 369 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7069 PvuII CAG'CTG Cuts at: 2860 2944 3076 2860 Size: 84 132 6853 Fragments arranged by size: 6853 132 84 SacI G_AGCT'C Cuts at: 2307 2934 2307 Size: 627 6442 SalI G'TCGA_C Cuts at: 1936 4091 4411 1936 Size: 2155 320 4594 Fragments arranged by size: 4594 2155 320 SanDI GG'GwC_CC Cuts at: 2794 2794 Size: 7069 SapI GCTCTTCn'nnn_ Cuts at: 2667 2909 2667 Size: 242 6827 SbfI CC_TGCA'GG Cuts at: 4421 4421 Size: 7069 ScaI AGT'ACT Cuts at: 5317 7029 5317 Size: 1712 5357 SfcI C'TryA_G Cuts at: 826 1045 2169 2651 3104 3344 3713 4417 5552 Size: 219 1124 482 453 240 369 704 1135 Cuts at: 5552 6233 6424 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1124 704 681 482 453 369 240 219 191 SfoI GGC'GCC Cuts at: 3036 3036 Size: 7069 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7069 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7069 SmaI CCC'GGG Cuts at: 1872 2101 3311 4396 1872 Size: 229 1210 1085 4545 Fragments arranged by size: 4545 1210 1085 229 SmlI C'TyrA_G Cuts at: 820 1017 1943 2306 2810 3978 5173 6074 6336 Size: 197 926 363 504 1168 1195 901 262 Cuts at: 6336 820 Size: 1553 Fragments arranged by size: 1553 1195 1168 926 901 504 363 262 197 SnaBI TAC'GTA Cuts at: 493 4860 493 Size: 4367 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7069 SphI G_CATG'C Cuts at: 1132 4427 1132 Size: 3295 3774 SspI AAT'ATT Cuts at: 5 52 2048 4993 5 Size: 47 1996 2945 2081 Fragments arranged by size: 2945 2081 1996 47 StuI AGG'CCT Cuts at: 1517 3888 1517 Size: 2371 4698 StyI C'CwwG_G Cuts at: 513 1065 2973 3465 3633 4508 513 Size: 552 1908 492 168 875 3074 Fragments arranged by size: 3074 1908 875 552 492 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2172 5096 5113 5266 5451 5896 325 Size: 1255 592 2924 17 153 185 445 1498 Fragments arranged by size: 2924 1498 1255 592 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2431 3554 4083 Size: 274 80 33 51 1443 454 1123 529 Cuts at: 4083 4233 5315 7027 96 Size: 150 1082 1712 138 Fragments arranged by size: 1712 1443 1123 1082 529 454 274 150 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2205 2237 3560 3592 3634 3666 3999 4031 7029 Size: 32 1323 32 42 32 333 32 2998 Cuts at: 7029 7061 2205 Size: 32 2213 Fragments arranged by size: 2998 2213 1323 333 42 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2066 2981 1316 Size: 750 915 5404 Fragments arranged by size: 5404 915 750 XbaI T'CTAG_A Cuts at: 4405 4405 Size: 7069 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3073 3073 Size: 7069 XhoI C'TCGA_G Cuts at: 1943 2810 3978 1943 Size: 867 1168 5034 Fragments arranged by size: 5034 1168 867 XmaI C'CCGG_G Cuts at: 1870 2099 3309 4394 1870 Size: 229 1210 1085 4545 Fragments arranged by size: 4545 1210 1085 229 XmnI GAAnn'nnTTC Cuts at: 5198 5198 Size: 7069 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4877 276 Size: 53 83 186 936 3343 2468 Fragments arranged by size: 3343 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII FalI FseI FspAI NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SexAI SfiI SrfI SwaI