(Circular) (Six-base) MAPSORT of: pFN21AB9776.seq Check: 7480 from: 1 to: 6853 pFN21AB9776 6853 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4663 278 Size: 53 83 186 936 3127 2468 Fragments arranged by size: 3127 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4196 1937 Size: 2259 4594 Acc65I G'GTAC_C Cuts at: 4174 4174 Size: 6853 AclI AA'CG_TT Cuts at: 1563 4980 5353 1563 Size: 3417 373 3063 Fragments arranged by size: 3417 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2692 4916 6300 6568 1632 Size: 1060 2224 1384 268 1917 Fragments arranged by size: 2224 1917 1384 1060 268 AfeI AGC'GCT Cuts at: 3875 3875 Size: 6853 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6656 AflIII A'CryG_T Cuts at: 1203 5752 6729 1203 Size: 4549 977 1327 Fragments arranged by size: 4549 1327 977 AgeI A'CCGG_T Cuts at: 5684 5684 Size: 6853 AhdI GACnn_n'nnGTC Cuts at: 3793 5582 3793 Size: 1789 5064 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6853 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3857 1184 Size: 2673 4180 AlwNI CAG_nnn'CTG Cuts at: 4596 6168 4596 Size: 1572 5281 ApaI G_GGCC'C Cuts at: 2988 4038 2988 Size: 1050 5803 ApaLI G'TGCA_C Cuts at: 2972 4910 6066 2972 Size: 1938 1156 3759 Fragments arranged by size: 3759 1938 1156 ApoI r'AATT_y Cuts at: 1493 4162 4388 4540 6649 1493 Size: 2669 226 152 2109 1697 Fragments arranged by size: 2669 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5407 160 Size: 872 4375 1606 Fragments arranged by size: 4375 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6853 AvaI C'yCGr_G Cuts at: 1870 1943 3583 3655 3955 4178 1870 Size: 73 1640 72 300 223 4545 Fragments arranged by size: 4545 1640 300 223 73 72 BamHI G'GATC_C Cuts at: 4183 4550 4183 Size: 367 6486 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3113 3545 3862 4174 5629 Size: 325 217 627 1326 432 317 312 1455 Cuts at: 5629 618 Size: 1842 Fragments arranged by size: 1842 1455 1326 627 432 325 317 312 217 BanII G_rGCy'C Cuts at: 1391 2255 2988 3060 3140 4038 4048 4172 1391 Size: 864 733 72 80 898 10 124 4072 Fragments arranged by size: 4072 898 864 733 124 80 72 10 BbeI G_GCGC'C Cuts at: 3549 3866 3549 Size: 317 6536 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2656 928 Size: 971 757 5125 Fragments arranged by size: 5125 971 757 BbvCI CC'TCA_GC Cuts at: 2247 2247 Size: 6853 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5044 5078 1903 Size: 34 3107 34 3678 Fragments arranged by size: 3678 3107 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4745 5955 4745 Size: 1210 5643 BclI T'GATC_A Cuts at: 1548 1548 Size: 6853 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3064 5464 136 Size: 107 122 71 631 605 1392 2400 1525 Fragments arranged by size: 2400 1525 1392 631 605 122 107 71 BglII A'GATC_T Cuts at: 2181 6848 2181 Size: 4667 2186 BlpI GC'TnA_GC Cuts at: 4270 4270 Size: 6853 Bme1580I G_kGCm'C Cuts at: 1790 2976 2988 3981 4038 4077 4914 6070 1790 Size: 1186 12 993 57 39 837 1156 2573 Fragments arranged by size: 2573 1186 1156 993 837 57 39 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6853 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4122 4584 5542 Size: 416 53 83 399 24 2705 462 958 Cuts at: 5542 442 Size: 1753 Fragments arranged by size: 2705 1753 958 462 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6853 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2200 2428 2608 2716 2200 Size: 228 180 108 6337 Fragments arranged by size: 6337 228 180 108 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2576 3523 5513 1239 Size: 1337 947 1990 2579 Fragments arranged by size: 2579 1990 1337 947 Bpu10I CC'TnA_GC Cuts at: 2247 3146 3877 2247 Size: 899 731 5223 Fragments arranged by size: 5223 899 731 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3290 4978 5843 6141 3290 Size: 1688 865 298 4002 Fragments arranged by size: 4002 1688 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2006 2552 3094 5516 882 Size: 617 507 546 542 2422 2219 Fragments arranged by size: 2422 2219 617 546 542 507 BsaAI yAC'GTr Cuts at: 493 1204 3029 4644 493 Size: 711 1825 1615 2702 Fragments arranged by size: 2702 1825 1615 711 BsaBI GATnn'nnATC Cuts at: 3314 6847 3314 Size: 3533 3320 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2048 3546 3863 4660 Size: 53 83 186 936 515 1498 317 797 Cuts at: 4660 5042 5677 275 Size: 382 635 1451 Fragments arranged by size: 1498 1451 936 797 635 515 382 317 186 83 53 BsaWI w'CCGG_w Cuts at: 3384 5286 5684 5958 6105 6516 3384 Size: 1902 398 274 147 411 3721 Fragments arranged by size: 3721 1902 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2036 2066 3521 3551 1178 Size: 30 165 30 633 30 1455 30 4480 Fragments arranged by size: 4480 1455 633 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 3160 1186 Size: 1006 968 4879 Fragments arranged by size: 4879 1006 968 BseYI C'CCAG_C Cuts at: 2123 2266 2427 3012 3670 3866 6056 6552 2123 Size: 143 161 585 658 196 2190 496 2424 Fragments arranged by size: 2424 2190 658 585 496 196 161 143 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6280 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3757 4303 5064 6092 1540 Size: 275 186 1756 546 761 1028 2301 Fragments arranged by size: 2301 1756 1028 761 546 275 186 BsiHKAI G_wGCw'C Cuts at: 2976 3152 3900 4914 4999 6070 2976 Size: 176 748 1014 85 1071 3759 Fragments arranged by size: 3759 1071 1014 748 176 85 BsmI GAATG_Cn' Cuts at: 4364 4457 4364 Size: 93 6760 BsmBI CGTCTCn'nnnn_ Cuts at: 2039 2399 2039 Size: 360 6493 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2255 2976 2988 3060 3140 3152 3900 Size: 399 465 721 12 72 80 12 748 Cuts at: 3900 3981 4038 4048 4077 4172 4914 4999 6070 Size: 81 57 10 29 95 742 85 1071 Cuts at: 6070 1391 Size: 2174 Fragments arranged by size: 2174 1071 748 742 721 465 399 95 85 81 80 72 57 29 12 12 10 BspEI T'CCGG_A Cuts at: 6516 6516 Size: 6853 BspHI T'CATG_A Cuts at: 2847 4742 6472 2847 Size: 1895 1730 3228 Fragments arranged by size: 3228 1895 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2592 3423 4208 844 Size: 1748 831 785 3489 Fragments arranged by size: 3489 1748 831 785 BsrBI CCG'CTC Cuts at: 1394 1823 2437 2695 3859 4740 1394 Size: 429 614 258 1164 881 3507 Fragments arranged by size: 3507 1164 881 614 429 258 BsrDI GCAATG_nn' Cuts at: 65 1248 3041 5348 5522 65 Size: 1183 1793 2307 174 1396 Fragments arranged by size: 2307 1793 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3110 3883 5497 5684 1700 Size: 110 1300 773 1614 187 2869 Fragments arranged by size: 2869 1614 1300 773 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6853 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6853 BssSI C'ACGA_G Cuts at: 4913 5925 4913 Size: 1012 5841 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6853 BstBI TT'CG_AA Cuts at: 5657 5657 Size: 6853 BstEII G'GTnAC_C Cuts at: 1184 3488 1184 Size: 2304 4549 BstXI CCAn_nnnn'nTGG Cuts at: 2036 2036 Size: 6853 BstYI r'GATC_y Cuts at: 1972 2181 4183 4550 4935 4952 6404 6490 6502 Size: 209 2002 367 385 17 1452 86 12 Cuts at: 6502 6848 1972 Size: 346 1977 Fragments arranged by size: 2002 1977 1452 385 367 346 209 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2862 3258 3603 3851 4023 4029 Size: 552 939 858 396 345 248 172 6 Cuts at: 4029 4116 513 Size: 87 3250 Fragments arranged by size: 3250 939 858 552 396 345 248 172 87 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6114 Fragments arranged by size: 6114 683 56 BtsI GCAGTG_nn' Cuts at: 2554 2635 3096 3476 3736 4369 5163 5183 2554 Size: 81 461 380 260 633 794 20 4224 Fragments arranged by size: 4224 794 633 461 380 260 81 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6853 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2797 2832 529 Size: 35 2233 35 4550 Fragments arranged by size: 4550 2233 35 35 DraI TTT'AAA Cuts at: 4157 4157 Size: 6853 DraIII CAC_nnn'GTG Cuts at: 3367 3367 Size: 6853 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2948 5860 809 Size: 507 1632 2912 1802 Fragments arranged by size: 2912 1802 1632 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2218 2438 3507 Size: 54 1425 50 275 18 388 220 1069 Cuts at: 3507 3888 3924 4026 4113 4300 5189 6818 8 Size: 381 36 102 87 187 889 1629 43 Fragments arranged by size: 1629 1425 1069 889 388 381 275 220 187 102 87 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 4300 1537 Size: 275 2488 4090 Fragments arranged by size: 4090 2488 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4783 1353 Size: 3430 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2111 2683 3425 5440 5814 5960 Size: 1064 138 522 572 742 2015 374 146 Cuts at: 5960 387 Size: 1280 Fragments arranged by size: 2015 1280 1064 742 572 522 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2576 2692 3523 4916 5513 6300 6568 Size: 393 944 116 831 1393 597 787 268 Cuts at: 6568 1239 Size: 1524 Fragments arranged by size: 1524 1393 944 831 787 597 393 268 116 EcoNI CCTnn'n_nnAGG Cuts at: 3273 3273 Size: 6853 EcoO109I rG'GnC_Cy Cuts at: 2984 3016 3772 4034 4035 2984 Size: 32 756 262 1 5802 Fragments arranged by size: 5802 756 262 32 1 EcoRI G'AATT_C Cuts at: 4162 4540 4162 Size: 378 6475 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2155 3475 2155 Size: 1320 5533 FspI TGC'GCA Cuts at: 5359 5359 Size: 6853 HaeII r_GCGC'y Cuts at: 1678 3205 3549 3699 3866 3877 6000 1678 Size: 1527 344 150 167 11 2123 2531 Fragments arranged by size: 2531 2123 1527 344 167 150 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2276 2308 2516 2548 3779 Size: 32 135 32 704 32 208 32 1231 Cuts at: 3779 3811 3949 3981 4052 4084 5494 5526 5568 Size: 32 138 32 71 32 1410 32 42 Cuts at: 5568 5600 1373 Size: 32 2626 Fragments arranged by size: 2626 1410 1231 704 208 138 135 71 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3448 4197 4443 669 Size: 988 281 1510 749 246 3079 Fragments arranged by size: 3079 1510 988 749 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6853 HpaI GTT'AAC Cuts at: 4443 4443 Size: 6853 KasI G'GCGC_C Cuts at: 3545 3862 3545 Size: 317 6536 KpnI G_GTAC'C Cuts at: 4178 4178 Size: 6853 MfeI C'AATT_G Cuts at: 4452 4452 Size: 6853 MluI A'CGCG_T Cuts at: 6729 6729 Size: 6853 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3261 3704 3762 5967 6151 1305 Size: 1956 443 58 2205 184 2007 Fragments arranged by size: 2205 2007 1956 443 184 58 MscI TGG'CCA Cuts at: 10 64 1489 1832 3509 3926 4115 10 Size: 54 1425 343 1677 417 189 2748 Fragments arranged by size: 2748 1677 1425 417 343 189 54 MslI CAynn'nnrTG Cuts at: 518 1166 2034 2361 2367 2540 4004 4115 4811 Size: 648 868 327 6 173 1464 111 696 Cuts at: 4811 5170 5329 518 Size: 359 159 2042 Fragments arranged by size: 2042 1464 868 696 648 359 327 173 159 111 6 MspA1I CmG'CkG Cuts at: 1606 2246 3807 3853 4025 4269 4946 6094 6339 Size: 640 1561 46 172 244 677 1148 245 Cuts at: 6339 1606 Size: 2120 Fragments arranged by size: 2120 1561 1148 677 640 245 244 172 46 NaeI GCC'GGC Cuts at: 3885 3885 Size: 6853 NarI GG'CG_CC Cuts at: 3546 3863 3546 Size: 317 6536 NcoI C'CATG_G Cuts at: 513 1065 2004 3258 3603 4029 4116 513 Size: 552 939 1254 345 426 87 3250 Fragments arranged by size: 3250 1254 939 552 426 345 87 NdeI CA'TA_TG Cuts at: 387 387 Size: 6853 NgoMIV G'CCGG_C Cuts at: 3883 3883 Size: 6853 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6853 NotI GC'GGCC_GC Cuts at: 4300 4300 Size: 6853 NspI r_CATG'y Cuts at: 1132 1229 2468 3301 4211 5756 1132 Size: 97 1239 833 910 1545 2229 Fragments arranged by size: 2229 1545 1239 910 833 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6853 PciI A'CATG_T Cuts at: 5752 5752 Size: 6853 PflMI CCAn_nnn'nTGG Cuts at: 1108 3012 3367 3922 1108 Size: 1904 355 555 4039 Fragments arranged by size: 4039 1904 555 355 PfoI T'CCnGG_A Cuts at: 3663 3970 3663 Size: 307 6546 PmeI GTTT'AAAC Cuts at: 4157 4157 Size: 6853 PmlI CAC'GTG Cuts at: 3029 3029 Size: 6853 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4922 4954 6435 6467 4922 Size: 32 1481 32 5308 Fragments arranged by size: 5308 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3016 3772 3016 Size: 756 6097 PshAI GACnn'nnGTC Cuts at: 1613 3021 1613 Size: 1408 5445 PsiI TTA'TAA Cuts at: 4423 4423 Size: 6853 PspOMI G'GGCC_C Cuts at: 2984 4034 2984 Size: 1050 5803 PstI C_TGCA'G Cuts at: 830 3103 4205 830 Size: 2273 1102 3478 Fragments arranged by size: 3478 2273 1102 PvuI CG_AT'CG Cuts at: 2001 3757 2001 Size: 1756 5097 PvuII CAG'CTG Cuts at: 2246 2246 Size: 6853 RsrII CG'GwC_CG Cuts at: 3382 3382 Size: 6853 SacII CC_GC'GG Cuts at: 3854 4026 3854 Size: 172 6681 SalI G'TCGA_C Cuts at: 1936 4195 1936 Size: 2259 4594 SanDI GG'GwC_CC Cuts at: 3016 3016 Size: 6853 SbfI CC_TGCA'GG Cuts at: 4205 4205 Size: 6853 ScaI AGT'ACT Cuts at: 5101 6813 5101 Size: 1712 5141 SfcI C'TryA_G Cuts at: 826 1045 3000 3099 3676 4201 5336 6017 6208 Size: 219 1955 99 577 525 1135 681 191 Cuts at: 6208 826 Size: 1471 Fragments arranged by size: 1955 1471 1135 681 577 525 219 191 99 SfoI GGC'GCC Cuts at: 3547 3864 3547 Size: 317 6536 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6853 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6853 SmaI CCC'GGG Cuts at: 1872 3585 3657 4180 1872 Size: 1713 72 523 4545 Fragments arranged by size: 4545 1713 523 72 SmlI C'TyrA_G Cuts at: 820 1017 1943 3305 4957 5858 6120 820 Size: 197 926 1362 1652 901 262 1553 Fragments arranged by size: 1652 1553 1362 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4644 493 Size: 4151 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6853 SphI G_CATG'C Cuts at: 1132 2468 4211 1132 Size: 1336 1743 3774 Fragments arranged by size: 3774 1743 1336 SspI AAT'ATT Cuts at: 5 52 4777 5 Size: 47 4725 2081 Fragments arranged by size: 4725 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6853 StyI C'CwwG_G Cuts at: 513 1065 2004 2091 3258 3596 3603 4029 4116 Size: 552 939 87 1167 338 7 426 87 Cuts at: 4116 4292 513 Size: 176 3074 Fragments arranged by size: 3074 1167 939 552 426 338 176 87 87 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2274 3654 4880 4897 5050 5235 5680 Size: 1255 694 1380 1226 17 153 185 445 Cuts at: 5680 325 Size: 1498 Fragments arranged by size: 1498 1380 1255 1226 694 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5099 6811 96 Size: 274 80 33 51 1443 3122 1712 138 Fragments arranged by size: 3122 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3521 3553 6813 6845 3521 Size: 32 3260 32 3529 Fragments arranged by size: 3529 3260 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6853 XbaI T'CTAG_A Cuts at: 4189 4189 Size: 6853 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2363 3530 2363 Size: 1167 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6853 XmaI C'CCGG_G Cuts at: 1870 3583 3655 4178 1870 Size: 1713 72 523 4545 Fragments arranged by size: 4545 1713 523 72 XmnI GAAnn'nnTTC Cuts at: 4982 4982 Size: 6853 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4661 276 Size: 53 83 186 936 3127 2468 Fragments arranged by size: 3127 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI SacI SapI SexAI SfiI SrfI SwaI