Restriction map for
pFN21AB9776
(Linear) (Six-base) MAPSORT of: KIBB9776.seq Check: 6395 from: 1 to: 2148
KIBB9776.orf
With 182 enzymes: SgfI *
September 18, 2009 17:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 2148
Size: 687 1461
AfeI AGC'GCT
Cuts at: 0 1870 2148
Size: 1870 278
AhdI GACnn_n'nnGTC
Cuts at: 0 1788 2148
Size: 1788 360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1852 2148
Size: 1852 296
ApaI G_GGCC'C
Cuts at: 0 983 2033 2148
Size: 983 1050 115
Fragments arranged by size:
1050 983 115
ApaLI G'TGCA_C
Cuts at: 0 967 2148
Size: 967 1181
AvaI C'yCGr_G
Cuts at: 0 1578 1650 1950 2148
Size: 1578 72 300 198
Fragments arranged by size:
1578 300 198 72
BanI G'GyrC_C
Cuts at: 0 1108 1540 1857 2148
Size: 1108 432 317 291
Fragments arranged by size:
1108 432 317 291
BanII G_rGCy'C
Cuts at: 0 250 983 1055 1135 2033 2043 2148
Size: 250 733 72 80 898 10 105
Fragments arranged by size:
898 733 250 105 80 72 10
BbeI G_GCGC'C
Cuts at: 0 1544 1861 2148
Size: 1544 317 287
Fragments arranged by size:
1544 317 287
BbsI GAAGACnn'nnnn_
Cuts at: 0 651 2148
Size: 651 1497
BbvCI CC'TCA_GC
Cuts at: 0 242 2148
Size: 242 1906
BglI GCCn_nnn'nGGC
Cuts at: 0 1059 2148
Size: 1059 1089
BglII A'GATC_T
Cuts at: 0 176 2148
Size: 176 1972
Bme1580I G_kGCm'C
Cuts at: 0 971 983 1976 2033 2072 2148
Size: 971 12 993 57 39 76
Fragments arranged by size:
993 971 76 57 39 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 2117 2148
Size: 2117 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 195 423 603 711 2148
Size: 195 228 180 108 1437
Fragments arranged by size:
1437 228 195 180 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1518 2148
Size: 571 947 630
Fragments arranged by size:
947 630 571
Bpu10I CC'TnA_GC
Cuts at: 0 242 1141 1872 2148
Size: 242 899 731 276
Fragments arranged by size:
899 731 276 242
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1285 2148
Size: 1285 863
BsaI GGTCTCn'nnnn_
Cuts at: 0 1 547 1089 2148
Size: 1 546 542 1059
Fragments arranged by size:
1059 546 542 1
BsaAI yAC'GTr
Cuts at: 0 1024 2148
Size: 1024 1124
BsaBI GATnn'nnATC
Cuts at: 0 1309 2148
Size: 1309 839
BsaHI Gr'CG_yC
Cuts at: 0 43 1541 1858 2148
Size: 43 1498 317 290
Fragments arranged by size:
1498 317 290 43
BsaWI w'CCGG_w
Cuts at: 0 1379 2148
Size: 1379 769
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 1516 1546 2148
Size: 31 30 1455 30 602
Fragments arranged by size:
1455 602 31 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 1155 2148
Size: 187 968 993
Fragments arranged by size:
993 968 187
BseYI C'CCAG_C
Cuts at: 0 118 261 422 1007 1665 1861 2148
Size: 118 143 161 585 658 196 287
Fragments arranged by size:
658 585 287 196 161 143 118
BsiEI CG_ry'CG
Cuts at: 0 1752 2148
Size: 1752 396
BsiHKAI G_wGCw'C
Cuts at: 0 971 1147 1895 2148
Size: 971 176 748 253
Fragments arranged by size:
971 748 253 176
BsmBI CGTCTCn'nnnn_
Cuts at: 0 34 394 2148
Size: 34 360 1754
Fragments arranged by size:
1754 360 34
Bsp1286I G_dGCh'C
Cuts at: 0 250 971 983 1055 1135 1147 1895 1976
Size: 250 721 12 72 80 12 748 81
Cuts at: 1976 2033 2043 2072 2148
Size: 57 10 29 76
Fragments arranged by size:
748 721 250 81 80 76 72 57
29 12 12 10
BspHI T'CATG_A
Cuts at: 0 842 2148
Size: 842 1306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 587 1418 2148
Size: 587 831 730
Fragments arranged by size:
831 730 587
BsrBI CCG'CTC
Cuts at: 0 432 690 1854 2148
Size: 432 258 1164 294
Fragments arranged by size:
1164 432 294 258
BsrDI GCAATG_nn'
Cuts at: 0 1036 2148
Size: 1036 1112
BsrFI r'CCGG_y
Cuts at: 0 1105 1878 2148
Size: 1105 773 270
Fragments arranged by size:
1105 773 270
BstEII G'GTnAC_C
Cuts at: 0 1483 2148
Size: 1483 665
BstXI CCAn_nnnn'nTGG
Cuts at: 0 31 2148
Size: 31 2117
BstYI r'GATC_y
Cuts at: 0 176 2148
Size: 176 1972
BtgI C'CryG_G
Cuts at: 0 857 1253 1598 1846 2018 2024 2111 2148
Size: 857 396 345 248 172 6 87 37
Fragments arranged by size:
857 396 345 248 172 87 37 6
BtsI GCAGTG_nn'
Cuts at: 0 549 630 1091 1471 1731 2148
Size: 549 81 461 380 260 417
Fragments arranged by size:
549 461 417 380 260 81
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 792 827 2148
Size: 792 35 1321
Fragments arranged by size:
1321 792 35
DraIII CAC_nnn'GTG
Cuts at: 0 1362 2148
Size: 1362 786
DrdI GACnn_nn'nnGTC
Cuts at: 0 943 2148
Size: 943 1205
EaeI y'GGCC_r
Cuts at: 0 213 433 1502 1883 1919 2021 2108 2148
Size: 213 220 1069 381 36 102 87 40
Fragments arranged by size:
1069 381 220 213 102 87 40 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 678 1420 2148
Size: 106 572 742 728
Fragments arranged by size:
742 728 572 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 687 1518 2148
Size: 571 116 831 630
Fragments arranged by size:
831 630 571 116
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1268 2148
Size: 1268 880
EcoO109I rG'GnC_Cy
Cuts at: 0 979 1011 1767 2029 2030 2148
Size: 979 32 756 262 1 118
Fragments arranged by size:
979 756 262 118 32 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 150 1470 2148
Size: 150 1320 678
Fragments arranged by size:
1320 678 150
HaeII r_GCGC'y
Cuts at: 0 1200 1544 1694 1861 1872 2148
Size: 1200 344 150 167 11 276
Fragments arranged by size:
1200 344 276 167 150 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 271 303 511 543 1774 1806 1944 1976
Size: 271 32 208 32 1231 32 138 32
Cuts at: 1976 2047 2079 2148
Size: 71 32 69
Fragments arranged by size:
1231 271 208 138 71 69 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1443 2148
Size: 1443 705
KasI G'GCGC_C
Cuts at: 0 1540 1857 2148
Size: 1540 317 291
Fragments arranged by size:
1540 317 291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1256 1699 1757 2148
Size: 1256 443 58 391
Fragments arranged by size:
1256 443 391 58
MscI TGG'CCA
Cuts at: 0 1504 1921 2110 2148
Size: 1504 417 189 38
Fragments arranged by size:
1504 417 189 38
MslI CAynn'nnrTG
Cuts at: 0 29 356 362 535 1999 2110 2148
Size: 29 327 6 173 1464 111 38
Fragments arranged by size:
1464 327 173 111 38 29 6
MspA1I CmG'CkG
Cuts at: 0 241 1802 1848 2020 2148
Size: 241 1561 46 172 128
Fragments arranged by size:
1561 241 172 128 46
NaeI GCC'GGC
Cuts at: 0 1880 2148
Size: 1880 268
NarI GG'CG_CC
Cuts at: 0 1541 1858 2148
Size: 1541 317 290
Fragments arranged by size:
1541 317 290
NcoI C'CATG_G
Cuts at: 0 1253 1598 2024 2111 2148
Size: 1253 345 426 87 37
Fragments arranged by size:
1253 426 345 87 37
NgoMIV G'CCGG_C
Cuts at: 0 1878 2148
Size: 1878 270
NspI r_CATG'y
Cuts at: 0 463 1296 2148
Size: 463 833 852
Fragments arranged by size:
852 833 463
PflMI CCAn_nnn'nTGG
Cuts at: 0 1007 1362 1917 2148
Size: 1007 355 555 231
Fragments arranged by size:
1007 555 355 231
PfoI T'CCnGG_A
Cuts at: 0 1658 1965 2148
Size: 1658 307 183
Fragments arranged by size:
1658 307 183
PmlI CAC'GTG
Cuts at: 0 1024 2148
Size: 1024 1124
PpuMI rG'GwC_Cy
Cuts at: 0 1011 1767 2148
Size: 1011 756 381
Fragments arranged by size:
1011 756 381
PshAI GACnn'nnGTC
Cuts at: 0 1016 2148
Size: 1016 1132
PspOMI G'GGCC_C
Cuts at: 0 979 2029 2148
Size: 979 1050 119
Fragments arranged by size:
1050 979 119
PstI C_TGCA'G
Cuts at: 0 1098 2148
Size: 1098 1050
PvuI CG_AT'CG
Cuts at: 0 1752 2148
Size: 1752 396
PvuII CAG'CTG
Cuts at: 0 241 2148
Size: 241 1907
RsrII CG'GwC_CG
Cuts at: 0 1377 2148
Size: 1377 771
SacII CC_GC'GG
Cuts at: 0 1849 2021 2148
Size: 1849 172 127
Fragments arranged by size:
1849 172 127
SanDI GG'GwC_CC
Cuts at: 0 1011 2148
Size: 1011 1137
SfcI C'TryA_G
Cuts at: 0 995 1094 1671 2148
Size: 995 99 577 477
Fragments arranged by size:
995 577 477 99
SfoI GGC'GCC
Cuts at: 0 1542 1859 2148
Size: 1542 317 289
Fragments arranged by size:
1542 317 289
SmaI CCC'GGG
Cuts at: 0 1580 1652 2148
Size: 1580 72 496
Fragments arranged by size:
1580 496 72
SmlI C'TyrA_G
Cuts at: 0 1300 2148
Size: 1300 848
SphI G_CATG'C
Cuts at: 0 463 2148
Size: 463 1685
StyI C'CwwG_G
Cuts at: 0 86 1253 1591 1598 2024 2111 2148
Size: 86 1167 338 7 426 87 37
Fragments arranged by size:
1167 426 338 87 86 37 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 269 1649 2148
Size: 269 1380 499
Fragments arranged by size:
1380 499 269
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1516 1548 2148
Size: 1516 32 600
Fragments arranged by size:
1516 600 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 358 1525 2148
Size: 358 1167 623
Fragments arranged by size:
1167 623 358
XmaI C'CCGG_G
Cuts at: 0 1578 1650 2148
Size: 1578 72 498
Fragments arranged by size:
1578 498 72
Enzymes that do cut:
AcuI AfeI AhdI AlfI ApaI ApaLI AvaI BanI
BanII BbeI BbsI BbvCI BglI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BstEII BstXI BstYI
BtgI BtsI CspCI DraIII DrdI EaeI EciI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacII SanDI SfcI SfoI SmaI SmlI
SphI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AloI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BlpI BmgBI
BmtI BsgI BsiWI BsmI BspEI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI DraI EagI
EarI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PasI PciI PmeI PpiI PsiI PspXI
PsrI SacI SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI