Restriction map for
pFN21AB9769
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AviII BamHI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BsaAI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BspEI BspLU11I BsrFI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Cfr10I DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MluI
MroI MspA1I MvnI NaeI NarI
NgoMIV NheI NotI NruI PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI PvuI PvuII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
StuI SwaI Tth111I Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 2578 GTCTAC GT^[AC][GT]AC
AflIII 971 ACGTGT A^C[GA][CT]GT
Alw44I 3070 GTGCAC G^TGCAC
ApaLI 3070 GTGCAC G^TGCAC
AvrII 1041 CCTAGG C^CTAGG
BanI 1818 GGCACC G^G[CT][GA]CC
BcnI 1822 CCCGG CC^[GC]GG
BlnI 1041 CCTAGG C^CTAGG
BlpI 2293 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2293 GCTGAGC GC^T[ACGT]AGC
BsaBI 1584 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1967 ACCGGA [AT]^CCGG[AT]
BspDI 1591 ATCGAT AT^CGAT
BspMI 2440 ACCTGC ACCTGC(4/8)
BsrBI 2536 GAGCGG CCGCTC(-3/-3)
BstBI 2749 TTCGAA TT^CGAA
CelII 2293 GCTGAGC GC^T[ACGT]AGC
CfoI 2467 GCGC GCG^C
ClaI 1591 ATCGAT AT^CGAT
CpoI 2999 CGGACCG CG^G[AT]CCG
DraI 2338 TTTAAA TTT^AAA
DraII 1343 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1343 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 487 GATATC GAT^ATC
EcoT22I 2269 ATGCAT ATGCA^T
FauI 2538 GCGGG CCCGC(4/6)
HgaI 22 GACGC GACGC(5/10)
HhaI 2467 GCGC GCG^C
HinP1I 2467 GCGC G^CGC
MamI 1584 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 57 CAATTG C^AATTG
MunI 57 CAATTG C^AATTG
NciI 1822 CCCGG CC^[GC]GG
NcoI 821 CCATGG C^CATGG
NsiI 2269 ATGCAT ATGCA^T
NspI 2115 GCATGC [GA]CATG^[CT]
NspV 2749 TTCGAA TT^CGAA
PaeR7I 1901 CTCGAG C^TCGAG
PpuMI 1343 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1776 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1406 CTGCAG CTGCA^G
RsrII 2999 CGGACCG CG^G[AT]CCG
SfuI 2749 TTCGAA TT^CGAA
SpeI 2458 ACTAGT A^CTAGT
SphI 2115 GCATGC GCATG^C
XcmI 2127 CCAGCCCTGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1901 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AclI 88 AACGTT AA^CGTT
1889 AACGTT
AlwI 877 GATCC GGATC(4/5)
2526 GATCC
AlwNI 1241 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
2816 CAGAGTCTG
Asp700I 1355 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1857 GAATGCTTTC
AvaI 1364 CTCGGG C^[CT]CG[GA]G
1901 CTCGAG
BalI 227 TGGCCA TGG^CCA
2488 TGGCCA
BbsI 2718 GAAGAC GAAGAC(2/6)
577 GTCTTC
BclI 1955 TGATCA T^GATCA
2609 TGATCA
BglII 1583 AGATCT A^GATCT
1989 AGATCT
BpuAI 2718 GAAGAC GAAGAC(2/6)
577 GTCTTC
BsmFI 2405 GGGAC GGGAC(10/14)
2694 GTCCC
BsoBI 1364 CTCGGG C^[CT]CG[GA]G
1901 CTCGAG
Bsp1407I 50 TGTACA T^GTACA
2099 TGTACA
BspHI 437 TCATGA T^CATGA
2714 TCATGA
BsrGI 50 TGTACA T^GTACA
2099 TGTACA
BstXI 2046 CCATGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2243 CCACGGACTTGG
BstYI 1583 AGATCT [GA]^GATC[CT]
1989 AGATCT
EaeI 227 TGGCCA [CT]^GGCC[GA]
2488 TGGCCA
EcoRI 1063 GAATTC G^AATTC
2658 GAATTC
EcoT14I 821 CCATGG C^C[AT][AT]GG
1041 CCTAGG
FbaI 1955 TGATCA T^GATCA
2609 TGATCA
MflI 1583 AGATCT [GA]^GATC[CT]
1989 AGATCT
MluNI 227 TGGCCA TGG^CCA
2488 TGGCCA
MscI 227 TGGCCA TGG^CCA
2488 TGGCCA
NdeI 793 CATATG CA^TATG
2266 CATATG
Psp1406I 88 AACGTT AA^CGTT
1889 AACGTT
RcaI 437 TCATGA T^CATGA
2714 TCATGA
SacI 535 GAGCTC GAGCT^C
2423 GAGCTC
SapI 441 GAAGAGC GCTCTTC(1/4)
1421 GAAGAGC
SspBI 50 TGTACA T^GTACA
2099 TGTACA
StyI 821 CCATGG C^C[AT][AT]GG
1041 CCTAGG
XhoII 1583 AGATCT [GA]^GATC[CT]
1989 AGATCT
XmnI 1355 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1857 GAATGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AocI
ApoI AspHI AvaII BanII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI Bsu36I Cac8I Cfr13I
DdeI DpnII DsaI EarI Eco81I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I