Restriction map for pFN21AB9769
 (Linear) (Six-base) MAPSORT of: KIBB9769.seq  Check: 5735  from: 1  to: 3141



KIBB9769.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AccI GT'mk_AC

Cuts at:      0    2579    3141 
   Size:       2579     562
 
AclI AA'CG_TT

Cuts at:      0      89    1890    3141 
   Size:         89    1801    1251

  Fragments arranged by size: 

                1801    1251      89
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     593     958    1132    1790    1837    1931    2434    2575 
   Size:        593     365     174     658      47      94     503     141

Cuts at:   2575    2822    2834    3141 
   Size:        247      12     307

  Fragments arranged by size: 

                 658     593     503     365     307     247     174     141
                  94      47      12
 
AflIII A'CryG_T

Cuts at:      0     971    3141 
   Size:        971    2170
 
AleI CACnn'nnGTG

Cuts at:      0    1258    2209    2227    3141 
   Size:       1258     951      18     914

  Fragments arranged by size: 

                1258     951     914      18
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2200    2232    3141 
   Size:       2200      32     909

  Fragments arranged by size: 

                2200     909      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1246    2821    3141 
   Size:       1246    1575     320

  Fragments arranged by size: 

                1575    1246     320
 
ApaLI G'TGCA_C

Cuts at:      0    3070    3141 
   Size:       3070      71
 
ApoI r'AATT_y

Cuts at:      0     421     586    1063    2343    2386    2658    3141 
   Size:        421     165     477    1280      43     272     483

  Fragments arranged by size: 

                1280     483     477     421     272     165      43
 
AvaI C'yCGr_G

Cuts at:      0    1364    1901    3141 
   Size:       1364     537    1240

  Fragments arranged by size: 

                1364    1240     537
 
AvrII C'CTAG_G

Cuts at:      0    1041    3141 
   Size:       1041    2100
 
BanI G'GyrC_C

Cuts at:      0    1818    3141 
   Size:       1818    1323
 
BanII G_rGCy'C

Cuts at:      0     300     539    1078    2427    3141 
   Size:        300     239     539    1349     714

  Fragments arranged by size: 

                1349     714     539     300     239
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     570    2725    3141 
   Size:        570    2155     416

  Fragments arranged by size: 

                2155     570     416
 
BclI T'GATC_A

Cuts at:      0    1955    2609    3141 
   Size:       1955     654     532

  Fragments arranged by size: 

                1955     654     532
 
BfrBI ATG'CAT

Cuts at:      0    2271    3141 
   Size:       2271     870
 
BglII A'GATC_T

Cuts at:      0    1583    1989    3141 
   Size:       1583     406    1152

  Fragments arranged by size: 

                1583    1152     406
 
BlpI GC'TnA_GC

Cuts at:      0    2294    3141 
   Size:       2294     847
 
Bme1580I G_kGCm'C

Cuts at:      0    3074    3141 
   Size:       3074      67
 
BmgBI CAC'GTC

Cuts at:      0     972     993    3141 
   Size:        972      21    2148

  Fragments arranged by size: 

                2148     972      21
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2302    3141 
   Size:       2302     839
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     449     901    2059    2794    2998    3141 
   Size:        449     452    1158     735     204     143

  Fragments arranged by size: 

                1158     735     452     449     204     143
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     748     869    2155    3141 
   Size:        748     121    1286     986

  Fragments arranged by size: 

                1286     986     748     121
 
BsaBI GATnn'nnATC

Cuts at:      0    1588    3141 
   Size:       1588    1553
 
BsaWI w'CCGG_w

Cuts at:      0    1967    3141 
   Size:       1967    1174
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2072    2102    3141 
   Size:       2072      30    1039

  Fragments arranged by size: 

                2072    1039      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     943    1333    1336    1339    2093    2890    3141 
   Size:        943     390       3       3     754     797     251

  Fragments arranged by size: 

                 943     797     754     390     251       3       3
 
BseYI C'CCAG_C

Cuts at:      0     694    1239    3141 
   Size:        694     545    1902

  Fragments arranged by size: 

                1902     694     545
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     515    2143    2233    3101    3141 
   Size:        515    1628      90     868      40

  Fragments arranged by size: 

                1628     868     515      90      40
 
BsiHKAI G_wGCw'C

Cuts at:      0     539    1428    1734    2427    3074    3141 
   Size:        539     889     306     693     647      67

  Fragments arranged by size: 

                 889     693     647     539     306      67
 
BsmI GAATG_Cn'

Cuts at:      0     291    1863    2181    3141 
   Size:        291    1572     318     960

  Fragments arranged by size: 

                1572     960     318     291
 
Bsp1286I G_dGCh'C

Cuts at:      0     300     539    1078    1428    1734    2427    3074    3141 
   Size:        300     239     539     350     306     693     647      67

  Fragments arranged by size: 

                 693     647     539     350     306     300     239      67
            
 
BspHI T'CATG_A

Cuts at:      0     437    2714    3141 
   Size:        437    2277     427

  Fragments arranged by size: 

                2277     437     427
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2449    3141 
   Size:       2449     692
 
BsrBI CCG'CTC

Cuts at:      0    2538    3141 
   Size:       2538     603
 
BsrDI GCAATG_nn'

Cuts at:      0     285    1631    1649    3141 
   Size:        285    1346      18    1492

  Fragments arranged by size: 

                1492    1346     285      18
 
BsrGI T'GTAC_A

Cuts at:      0      50    2099    3141 
   Size:         50    2049    1042

  Fragments arranged by size: 

                2049    1042      50
 
BstBI TT'CG_AA

Cuts at:      0    2750    3141 
   Size:       2750     391
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2053    2250    3141 
   Size:       2053     197     891

  Fragments arranged by size: 

                2053     891     197
 
BstYI r'GATC_y

Cuts at:      0    1583    1989    3141 
   Size:       1583     406    1152

  Fragments arranged by size: 

                1583    1152     406
 
Bsu36I CC'TnA_GG

Cuts at:      0    1556    2738    2780    3141 
   Size:       1556    1182      42     361

  Fragments arranged by size: 

                1556    1182     361      42
 
BtgI C'CryG_G

Cuts at:      0     821    1679    2243    3084    3096    3141 
   Size:        821     858     564     841      12      45

  Fragments arranged by size: 

                 858     841     821     564      45      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     498    3141 
   Size:        498    2643
 
BtsI GCAGTG_nn'

Cuts at:      0     310    1734    3141 
   Size:        310    1424    1407

  Fragments arranged by size: 

                1424    1407     310
 
ClaI AT'CG_AT

Cuts at:      0    1592    3141 
   Size:       1592    1549
 
DraI TTT'AAA

Cuts at:      0    2340    3141 
   Size:       2340     801
 
EaeI y'GGCC_r

Cuts at:      0     227    2488    3141 
   Size:        227    2261     653

  Fragments arranged by size: 

                2261     653     227
 
EarI CTCTTCn'nnn_

Cuts at:      0      37     436     671    1178    1310    1350    1406    1416 
   Size:         37     399     235     507     132      40      56      10

Cuts at:   1416    1907    2861    2867    2906    2945    3049    3141 
   Size:        491     954       6      39      39     104      92

  Fragments arranged by size: 

                 954     507     491     399     235     132     104      92
                  56      40      39      39      37      10       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      78    3141 
   Size:         78    3063
 
EcoICRI GAG'CTC

Cuts at:      0     537    2425    3141 
   Size:        537    1888     716

  Fragments arranged by size: 

                1888     716     537
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     449     593     901     958    1132    1790    1837    1931 
   Size:        449     144     308      57     174     658      47      94

Cuts at:   1931    2059    2434    2575    2794    2822    2834    2998    3141 
   Size:        128     375     141     219      28      12     164     143

  Fragments arranged by size: 

                 658     449     375     308     219     174     164     144
                 143     141     128      94      57      47      28      12
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1344    3141 
   Size:       1344    1797
 
EcoRI G'AATT_C

Cuts at:      0    1063    2658    3141 
   Size:       1063    1595     483

  Fragments arranged by size: 

                1595    1063     483
 
EcoRV GAT'ATC

Cuts at:      0     489    3141 
   Size:        489    2652
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     366    3141 
   Size:        366    2775
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     861     893    2803    2829    2835    2861    2884    2916 
   Size:        861      32    1910      26       6      26      23      32

Cuts at:   2916    3141 
   Size:        225

  Fragments arranged by size: 

                1910     861     225      32      32      26      26      23
                   6
 
MfeI C'AATT_G

Cuts at:      0      57    3141 
   Size:         57    3084
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     875    3141 
   Size:        875    2266
 
MscI TGG'CCA

Cuts at:      0     229    2490    3141 
   Size:        229    2261     651

  Fragments arranged by size: 

                2261     651     229
 
MslI CAynn'nnrTG

Cuts at:      0     223     868     991    1258    2051    2207    2209    2227 
   Size:        223     645     123     267     793     156       2      18

Cuts at:   2227    2270    3141 
   Size:         43     871

  Fragments arranged by size: 

                 871     793     645     267     223     156     123      43
                  18       2
 
NcoI C'CATG_G

Cuts at:      0     821    3141 
   Size:        821    2320
 
NdeI CA'TA_TG

Cuts at:      0     794    2267    3141 
   Size:        794    1473     874

  Fragments arranged by size: 

                1473     874     794
 
NsiI A_TGCA'T

Cuts at:      0    2273    3141 
   Size:       2273     868
 
NspI r_CATG'y

Cuts at:      0    2119    3141 
   Size:       2119    1022
 
PfoI T'CCnGG_A

Cuts at:      0    2771    3141 
   Size:       2771     370
 
PpuMI rG'GwC_Cy

Cuts at:      0    1344    3141 
   Size:       1344    1797
 
PshAI GACnn'nnGTC

Cuts at:      0    1780    3141 
   Size:       1780    1361
 
PsiI TTA'TAA

Cuts at:      0     905    1895    3141 
   Size:        905     990    1246

  Fragments arranged by size: 

                1246     990     905
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1820    1852    3141 
   Size:       1820      32    1289

  Fragments arranged by size: 

                1820    1289      32
 
PstI C_TGCA'G

Cuts at:      0    1410    3141 
   Size:       1410    1731
 
RsrII CG'GwC_CG

Cuts at:      0    3000    3141 
   Size:       3000     141
 
SacI G_AGCT'C

Cuts at:      0     539    2427    3141 
   Size:        539    1888     714

  Fragments arranged by size: 

                1888     714     539
 
SapI GCTCTTCn'nnn_

Cuts at:      0     436    1416    3141 
   Size:        436     980    1725

  Fragments arranged by size: 

                1725     980     436
 
SfcI C'TryA_G

Cuts at:      0    1406    2430    2589    2624    3141 
   Size:       1406    1024     159      35     517

  Fragments arranged by size: 

                1406    1024     517     159      35
 
SmlI C'TyrA_G

Cuts at:      0     763     884    1901    2170    3141 
   Size:        763     121    1017     269     971

  Fragments arranged by size: 

                1017     971     763     269     121
 
SpeI A'CTAG_T

Cuts at:      0    2458    3141 
   Size:       2458     683
 
SphI G_CATG'C

Cuts at:      0    2119    3141 
   Size:       2119    1022
 
SspI AAT'ATT

Cuts at:      0    1812    2010    2109    3141 
   Size:       1812     198      99    1032

  Fragments arranged by size: 

                1812    1032     198      99
 
StyI C'CwwG_G

Cuts at:      0     821    1041    3141 
   Size:        821     220    2100

  Fragments arranged by size: 

                2100     821     220
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     103    2291    3017    3141 
   Size:        103    2188     726     124

  Fragments arranged by size: 

                2188     726     124     103
 
TatI w'GTAC_w

Cuts at:      0      50    1881    2099    2688    3141 
   Size:         50    1831     218     589     453

  Fragments arranged by size: 

                1831     589     453     218      50
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2072    2104    3141 
   Size:       2072      32    1037

  Fragments arranged by size: 

                2072    1037      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2134    3141 
   Size:       2134    1007
 
XhoI C'TCGA_G

Cuts at:      0    1901    3141 
   Size:       1901    1240
 
XmnI GAAnn'nnTTC

Cuts at:      0    1359    1861    3141 
   Size:       1359     502    1280

  Fragments arranged by size: 

                1359    1280     502
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     AleI     AloI    AlwNI    ApaLI
     ApoI     AvaI    AvrII     BanI    BanII     BbsI     BclI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BplI     BpmI    BpuEI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BstBI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI     EaeI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    Hin4I
     MfeI     MmeI     MscI     MslI     NcoI     NdeI     NsiI     NspI
     PfoI    PpuMI    PshAI     PsiI     PsrI     PstI    RsrII     SacI
     SapI     SfcI     SmlI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AlfI
     ApaI     AscI     AseI    AsiSI     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI     BglI     BmrI     BmtI   Bpu10I     BsaI    BsaAI
    BsaHI    BsiEI    BsiWI    BsmBI    BspEI    BsrFI   BssHII    BssSI
   BstAPI   BstEII  BstZ17I    CspCI   DraIII     DrdI     EagI    EcoNI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MluI   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
   PspOMI    PspXI     PvuI    PvuII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SrfI     StuI     SwaI  Tth111I     XbaI     XmaI     ZraI
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