(Circular) (Six-base) MAPSORT of: pFN21AB9743.seq Check: 7060 from: 1 to: 6277 pFN21AB9743 6277 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2442 2442 Size: 6277 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4087 278 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3620 1937 Size: 1683 4594 Acc65I G'GTAC_C Cuts at: 3598 3598 Size: 6277 AclI AA'CG_TT Cuts at: 1563 3576 4404 4777 1563 Size: 2013 828 373 3063 Fragments arranged by size: 3063 2013 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 2774 4340 5724 5992 1632 Size: 422 720 1566 1384 268 1917 Fragments arranged by size: 1917 1566 1384 720 422 268 AfeI AGC'GCT Cuts at: 2822 2912 2822 Size: 90 6187 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6080 AflIII A'CryG_T Cuts at: 1203 5176 6153 1203 Size: 3973 977 1327 Fragments arranged by size: 3973 1327 977 AgeI A'CCGG_T Cuts at: 2766 5108 2766 Size: 2342 3935 AhdI GACnn_n'nnGTC Cuts at: 5006 5006 Size: 6277 AleI CACnn'nnGTG Cuts at: 1166 2778 1166 Size: 1612 4665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2454 1184 Size: 1270 5007 AlwNI CAG_nnn'CTG Cuts at: 2362 4020 5592 2362 Size: 1658 1572 3047 Fragments arranged by size: 3047 1658 1572 ApaI G_GGCC'C Cuts at: 2428 3058 3203 3370 2428 Size: 630 145 167 5335 Fragments arranged by size: 5335 630 167 145 ApaLI G'TGCA_C Cuts at: 4334 5490 4334 Size: 1156 5121 ApoI r'AATT_y Cuts at: 1493 3586 3812 3964 6073 1493 Size: 2093 226 152 2109 1697 Fragments arranged by size: 2109 2093 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4831 160 Size: 872 3799 1606 Fragments arranged by size: 3799 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 AvaI C'yCGr_G Cuts at: 1870 1943 2201 2303 2591 3602 1870 Size: 73 258 102 288 1011 4545 Fragments arranged by size: 4545 1011 288 258 102 73 BamHI G'GATC_C Cuts at: 3607 3974 3607 Size: 367 5910 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3355 3458 3598 5053 618 Size: 325 217 627 1568 103 140 1455 1842 Fragments arranged by size: 1842 1568 1455 627 325 217 140 103 BanII G_rGCy'C Cuts at: 1391 2428 2547 2988 3058 3203 3370 3548 3596 Size: 1037 119 441 70 145 167 178 48 Cuts at: 3596 1391 Size: 4072 Fragments arranged by size: 4072 1037 441 178 167 145 119 70 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5306 BbvCI CC'TCA_GC Cuts at: 2246 2246 Size: 6277 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2582 2616 4468 4502 1903 Size: 34 50 34 561 34 1852 34 3678 Fragments arranged by size: 3678 1852 561 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4169 5379 4169 Size: 1210 5067 BclI T'GATC_A Cuts at: 1548 1548 Size: 6277 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3108 4888 136 Size: 107 122 71 631 605 1436 1780 1525 Fragments arranged by size: 1780 1525 1436 631 605 122 107 71 BglII A'GATC_T Cuts at: 6272 6272 Size: 6277 BlpI GC'TnA_GC Cuts at: 3694 3694 Size: 6277 Bme1580I G_kGCm'C Cuts at: 1790 2340 2428 3058 3154 3203 3360 3370 4338 Size: 550 88 630 96 49 157 10 968 Cuts at: 4338 5494 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 968 630 550 157 96 88 49 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6277 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2334 2629 3290 Size: 416 53 83 399 24 917 295 661 Cuts at: 3290 4008 4966 442 Size: 718 958 1753 Fragments arranged by size: 1753 958 917 718 661 416 399 295 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 2316 2765 2818 4937 1239 Size: 903 174 449 53 2119 2579 Fragments arranged by size: 2579 2119 903 449 174 53 Bpu10I CC'TnA_GC Cuts at: 2246 2246 Size: 6277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4402 5267 5565 4402 Size: 865 298 5114 Fragments arranged by size: 5114 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3301 4940 882 Size: 617 1802 1639 2219 Fragments arranged by size: 2219 1802 1639 617 BsaAI yAC'GTr Cuts at: 493 1204 2869 4068 493 Size: 711 1665 1199 2702 Fragments arranged by size: 2702 1665 1199 711 BsaBI GATnn'nnATC Cuts at: 2672 3065 6271 2672 Size: 393 3206 2678 Fragments arranged by size: 3206 2678 393 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3392 3483 4084 4466 Size: 53 83 186 936 1859 91 601 382 Cuts at: 4466 5101 275 Size: 635 1451 Fragments arranged by size: 1859 1451 936 635 601 382 186 91 83 53 BsaWI w'CCGG_w Cuts at: 2766 4710 5108 5382 5529 5940 2766 Size: 1944 398 274 147 411 3103 Fragments arranged by size: 3103 1944 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6052 Fragments arranged by size: 6052 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2434 1186 Size: 1248 5029 BseYI C'CCAG_C Cuts at: 2086 2122 2384 2517 2924 3076 3525 5480 5976 Size: 36 262 133 407 152 449 1955 496 Cuts at: 5976 2086 Size: 2387 Fragments arranged by size: 2387 1955 496 449 407 262 152 133 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2096 2414 1163 Size: 573 360 318 5026 Fragments arranged by size: 5026 573 360 318 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2771 3727 4488 5516 1540 Size: 275 186 770 956 761 1028 2301 Fragments arranged by size: 2301 1028 956 770 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2110 2547 2988 4338 4423 5494 2110 Size: 437 441 1350 85 1071 2893 Fragments arranged by size: 2893 1350 1071 441 437 85 BsmI GAATG_Cn' Cuts at: 3788 3881 3788 Size: 93 6184 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2110 2340 2428 2547 2988 3058 3154 Size: 399 320 230 88 119 441 70 96 Cuts at: 3154 3203 3360 3370 3548 3596 4338 4423 5494 Size: 49 157 10 178 48 742 85 1071 Cuts at: 5494 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 441 399 320 230 178 157 119 96 88 85 70 49 48 10 BspEI T'CCGG_A Cuts at: 5940 5940 Size: 6277 BspHI T'CATG_A Cuts at: 4166 5896 4166 Size: 1730 4547 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2334 2442 2496 2729 3632 844 Size: 1490 108 54 233 903 3489 Fragments arranged by size: 3489 1490 903 233 108 54 BsrBI CCG'CTC Cuts at: 1394 1823 2026 4164 1394 Size: 429 203 2138 3507 Fragments arranged by size: 3507 2138 429 203 BsrDI GCAATG_nn' Cuts at: 65 1248 4772 4946 65 Size: 1183 3524 174 1396 Fragments arranged by size: 3524 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2766 3562 4921 5108 1700 Size: 110 956 796 1359 187 2869 Fragments arranged by size: 2869 1359 956 796 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6277 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6277 BssSI C'ACGA_G Cuts at: 2237 2972 4337 5349 2237 Size: 735 1365 1012 3165 Fragments arranged by size: 3165 1365 1012 735 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6277 BstBI TT'CG_AA Cuts at: 5081 5081 Size: 6277 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6277 BstXI CCAn_nnnn'nTGG Cuts at: 2506 3370 2506 Size: 864 5413 BstYI r'GATC_y Cuts at: 1972 2757 3607 3974 4359 4376 5828 5914 5926 Size: 785 850 367 385 17 1452 86 12 Cuts at: 5926 6272 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 850 785 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2173 2173 Size: 6277 BtgI C'CryG_G Cuts at: 513 1065 2638 2953 3477 513 Size: 552 1573 315 524 3313 Fragments arranged by size: 3313 1573 552 524 315 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5538 Fragments arranged by size: 5538 683 56 BtsI GCAGTG_nn' Cuts at: 2360 2930 3017 3184 3793 4587 4607 2360 Size: 570 87 167 609 794 20 4030 Fragments arranged by size: 4030 794 609 570 167 87 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6277 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6242 DraI TTT'AAA Cuts at: 3581 3581 Size: 6277 DraIII CAC_nnn'GTG Cuts at: 2869 2965 2869 Size: 96 6181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5284 809 Size: 507 3968 1802 Fragments arranged by size: 3968 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2641 2956 3237 Size: 54 1425 50 275 18 811 315 281 Cuts at: 3237 3425 3724 4613 6242 8 Size: 188 299 889 1629 43 Fragments arranged by size: 1629 1425 889 811 315 299 281 275 188 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3724 1537 Size: 275 1912 4090 Fragments arranged by size: 4090 1912 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4207 1353 Size: 2854 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3291 4864 5238 5384 387 Size: 1064 138 1702 1573 374 146 1280 Fragments arranged by size: 1702 1573 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2545 2986 2545 Size: 441 5836 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2142 2316 2765 2774 2818 4340 Size: 393 422 88 174 449 9 44 1522 Cuts at: 4340 4937 5724 5992 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1522 787 597 449 422 393 268 174 88 44 9 EcoO109I rG'GnC_Cy Cuts at: 2323 2424 2425 3054 3095 3200 3215 3366 3367 Size: 101 1 629 41 105 15 151 1 Cuts at: 3367 2323 Size: 5233 Fragments arranged by size: 5233 629 151 105 101 41 15 1 1 EcoRI G'AATT_C Cuts at: 3586 3964 3586 Size: 378 5899 FspI TGC'GCA Cuts at: 2936 4783 2936 Size: 1847 4430 HaeII r_GCGC'y Cuts at: 1678 2311 2824 2914 5424 1678 Size: 633 513 90 2510 2531 Fragments arranged by size: 2531 2510 633 513 90 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2912 2944 4918 4950 4992 Size: 32 135 32 1340 32 1974 32 42 Cuts at: 4992 5024 1373 Size: 32 2626 Fragments arranged by size: 2626 1974 1340 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2572 3487 3621 3867 669 Size: 988 281 634 915 134 246 3079 Fragments arranged by size: 3079 988 915 634 281 246 134 HindIII A'AGCT_T Cuts at: 748 748 Size: 6277 HpaI GTT'AAC Cuts at: 3867 3867 Size: 6277 KpnI G_GTAC'C Cuts at: 3602 3602 Size: 6277 MfeI C'AATT_G Cuts at: 3876 3876 Size: 6277 MluI A'CGCG_T Cuts at: 6153 6153 Size: 6277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2741 2853 5391 5575 1305 Size: 1436 112 2538 184 2007 Fragments arranged by size: 2538 2007 1436 184 112 MscI TGG'CCA Cuts at: 10 64 1489 1832 2958 3239 10 Size: 54 1425 343 1126 281 3048 Fragments arranged by size: 3048 1425 1126 343 281 54 MslI CAynn'nnrTG Cuts at: 518 1166 2778 4235 4594 4753 518 Size: 648 1612 1457 359 159 2042 Fragments arranged by size: 2042 1612 1457 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2506 2712 2995 3080 3250 3317 3417 3494 Size: 900 206 283 85 170 67 100 77 Cuts at: 3494 3693 4370 5518 5763 1606 Size: 199 677 1148 245 2120 Fragments arranged by size: 2120 1148 900 677 283 245 206 199 170 100 85 77 67 NcoI C'CATG_G Cuts at: 513 1065 2638 2953 513 Size: 552 1573 315 3837 Fragments arranged by size: 3837 1573 552 315 NdeI CA'TA_TG Cuts at: 387 387 Size: 6277 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6277 NotI GC'GGCC_GC Cuts at: 3724 3724 Size: 6277 NspI r_CATG'y Cuts at: 1132 1229 3635 5180 1132 Size: 97 2406 1545 2229 Fragments arranged by size: 2406 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3203 3370 1348 Size: 1855 167 4255 Fragments arranged by size: 4255 1855 167 PciI A'CATG_T Cuts at: 5176 5176 Size: 6277 PflMI CCAn_nnn'nTGG Cuts at: 1108 2264 2869 1108 Size: 1156 605 4516 Fragments arranged by size: 4516 1156 605 PmeI GTTT'AAAC Cuts at: 3581 3581 Size: 6277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4346 4378 5859 5891 4346 Size: 32 1481 32 4732 Fragments arranged by size: 4732 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2323 2323 Size: 6277 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6277 PsiI TTA'TAA Cuts at: 3847 3847 Size: 6277 PspOMI G'GGCC_C Cuts at: 2424 3054 3199 3366 2424 Size: 630 145 167 5335 Fragments arranged by size: 5335 630 167 145 PstI C_TGCA'G Cuts at: 830 2726 2994 3629 830 Size: 1896 268 635 3478 Fragments arranged by size: 3478 1896 635 268 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6277 PvuII CAG'CTG Cuts at: 2506 2995 3080 3317 3417 3494 2506 Size: 489 85 237 100 77 5289 Fragments arranged by size: 5289 489 237 100 85 77 SacI G_AGCT'C Cuts at: 2547 2988 2547 Size: 441 5836 SalI G'TCGA_C Cuts at: 1936 3619 1936 Size: 1683 4594 SbfI CC_TGCA'GG Cuts at: 3629 3629 Size: 6277 ScaI AGT'ACT Cuts at: 4525 6237 4525 Size: 1712 4565 SexAI A'CCwGG_T Cuts at: 2556 2556 Size: 6277 SfcI C'TryA_G Cuts at: 826 1045 2722 2990 3625 4760 5441 5632 826 Size: 219 1677 268 635 1135 681 191 1471 Fragments arranged by size: 1677 1471 1135 681 635 268 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6277 SmaI CCC'GGG Cuts at: 1872 3604 1872 Size: 1732 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4381 5282 5544 820 Size: 197 926 2438 901 262 1553 Fragments arranged by size: 2438 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4068 493 Size: 3575 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6277 SphI G_CATG'C Cuts at: 1132 3635 1132 Size: 2503 3774 SspI AAT'ATT Cuts at: 5 52 4201 5 Size: 47 4149 2081 Fragments arranged by size: 4149 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6277 StyI C'CwwG_G Cuts at: 513 1065 2463 2638 2953 3716 513 Size: 552 1398 175 315 763 3074 Fragments arranged by size: 3074 1398 763 552 315 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2078 2876 3517 4304 4321 4474 4659 Size: 1255 498 798 641 787 17 153 185 Cuts at: 4659 5104 325 Size: 445 1498 Fragments arranged by size: 1498 1255 798 787 641 498 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4523 6235 96 Size: 274 80 33 51 1443 2546 1712 138 Fragments arranged by size: 2546 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2074 2106 2951 2983 6237 6269 2074 Size: 32 845 32 3254 32 2082 Fragments arranged by size: 3254 2082 845 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2567 1316 Size: 1251 5026 XbaI T'CTAG_A Cuts at: 3613 3613 Size: 6277 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3367 2011 Size: 1356 4921 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6277 XmaI C'CCGG_G Cuts at: 1870 3602 1870 Size: 1732 4545 XmnI GAAnn'nnTTC Cuts at: 4406 4406 Size: 6277 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4085 276 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI