Restriction map for pFN21AB9685
 (Circular) (Six-base) MAPSORT of: pFN21AB9685.seq  Check: 8761  from: 1  to: 5290



pFN21AB9685  5290 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:28  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3100     278 
   Size:         53      83     186     936    1564    2468

  Fragments arranged by size: 

                2468    1564     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2633    1937 
   Size:        696    4594
 
Acc65I G'GTAC_C

Cuts at:   2611    2611 
   Size:       5290
 
AclI AA'CG_TT

Cuts at:   1563    3417    3790    1563 
   Size:       1854     373    3063

  Fragments arranged by size: 

                3063    1854     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3353    4737    5005    1632 
   Size:       1721    1384     268    1917

  Fragments arranged by size: 

                1917    1721    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5093
 
AflIII A'CryG_T

Cuts at:   1203    4189    5166    1203 
   Size:       2986     977    1327

  Fragments arranged by size: 

                2986    1327     977
 
AgeI A'CCGG_T

Cuts at:   4121    4121 
   Size:       5290
 
AhdI GACnn_n'nnGTC

Cuts at:   2123    4019    2123 
   Size:       1896    3394
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5290
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5290
 
AlwNI CAG_nnn'CTG

Cuts at:   3033    4605    3033 
   Size:       1572    3718
 
ApaLI G'TGCA_C

Cuts at:   3347    4503    3347 
   Size:       1156    4134
 
ApoI r'AATT_y

Cuts at:   1493    2018    2039    2083    2479    2583    2599    2825    2977 
   Size:        525      21      44     396     104      16     226     152

Cuts at:   2977    5086    1493 
   Size:       2109    1697

  Fragments arranged by size: 

                2109    1697     525     396     226     152     104      44
                  21      16
 
AseI AT'TA_AT

Cuts at:    160    1032    3844     160 
   Size:        872    2812    1606

  Fragments arranged by size: 

                2812    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5290
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2615    1870 
   Size:         73     672    4545

  Fragments arranged by size: 

                4545     672      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2439    2472    2439 
   Size:         33    5257
 
BamHI G'GATC_C

Cuts at:   2620    2987    2620 
   Size:        367    4923
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2611    4066     618 
   Size:        325     217     627     824    1455    1842

  Fragments arranged by size: 

                1842    1455     824     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2609    1391 
   Size:       1218    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2119     928 
   Size:        971     220    4099

  Fragments arranged by size: 

                4099     971     220
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3481    3515    1903 
   Size:         34    1544      34    3678

  Fragments arranged by size: 

                3678    1544      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3182    4392    3182 
   Size:       1210    4080
 
BclI T'GATC_A

Cuts at:   1548    2160    1548 
   Size:        612    4678
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3901     136 
   Size:        107     122      71     631     605    2229    1525

  Fragments arranged by size: 

                2229    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5285    5285 
   Size:       5290
 
BlpI GC'TnA_GC

Cuts at:   2707    2707 
   Size:       5290
 
Bme1580I G_kGCm'C

Cuts at:   1790    3351    4507    1790 
   Size:       1561    1156    2573

  Fragments arranged by size: 

                2573    1561    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5290
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2222    3021    3979 
   Size:        416      53      83     399      24     805     799     958

Cuts at:   3979     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     805     799     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5290
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2069    2192    3950    1239 
   Size:        830     123    1758    2579

  Fragments arranged by size: 

                2579    1758     830     123
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3415    4280    4578    3415 
   Size:        865     298    4127

  Fragments arranged by size: 

                4127     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3953     882 
   Size:        617    2454    2219

  Fragments arranged by size: 

                2454    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3081     493 
   Size:        711    1877    2702

  Fragments arranged by size: 

                2702    1877     711
 
BsaBI GATnn'nnATC

Cuts at:   5284    5284 
   Size:       5290
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3097    3479    4114     275 
   Size:         53      83     186     936    1564     382     635    1451

  Fragments arranged by size: 

                1564    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3723    4121    4395    4542    4953    3723 
   Size:        398     274     147     411    4060

  Fragments arranged by size: 

                4060     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5065

  Fragments arranged by size: 

                5065     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2066    1186 
   Size:        880    4410
 
BseYI C'CCAG_C

Cuts at:   4493    4989    4493 
   Size:        496    4794
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2058    1163 
   Size:        573     322    4395

  Fragments arranged by size: 

                4395     573     322
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2740    3501    4529    1540 
   Size:        275     186     739     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     739     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3351    3436    4507    3351 
   Size:         85    1071    4134

  Fragments arranged by size: 

                4134    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2801    2894    2801 
   Size:         93    5197
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2609    3351    3436    4507    1391 
   Size:        399     819     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     819     742     399      85
 
BspEI T'CCGG_A

Cuts at:   4953    4953 
   Size:       5290
 
BspHI T'CATG_A

Cuts at:   3179    4909    3179 
   Size:       1730    3560
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2645     844 
   Size:       1801    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3177    1394 
   Size:        429    1354    3507

  Fragments arranged by size: 

                3507    1354     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2059    3785    3959      65 
   Size:       1183     811    1726     174    1396

  Fragments arranged by size: 

                1726    1396    1183     811     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3934    4121    1700 
   Size:        110    2124     187    2869

  Fragments arranged by size: 

                2869    2124     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5290
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5290
 
BssSI C'ACGA_G

Cuts at:   3350    4362    3350 
   Size:       1012    4278
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5290
 
BstBI TT'CG_AA

Cuts at:   4094    4094 
   Size:       5290
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5290
 
BstYI r'GATC_y

Cuts at:   1972    2620    2987    3372    3389    4841    4927    4939    5285 
   Size:        648     367     385      17    1452      86      12     346

Cuts at:   5285    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     648     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3799

  Fragments arranged by size: 

                3799     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4551

  Fragments arranged by size: 

                4551     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2070    2806    3600    3620    2070 
   Size:        736     794      20    3740

  Fragments arranged by size: 

                3740     794     736      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5290
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5255
 
DraI TTT'AAA

Cuts at:   2594    2594 
   Size:       5290
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4297     809 
   Size:        507    2981    1802

  Fragments arranged by size: 

                2981    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2031    2737    3626 
   Size:         54    1425      50     275      18     201     706     889

Cuts at:   3626    5255       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     706     275     201      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2737    1537 
   Size:        275     925    4090

  Fragments arranged by size: 

                4090     925     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2195    3220    1353 
   Size:        842    1025    3423

  Fragments arranged by size: 

                3423    1025     842
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3877    4251    4397     387 
   Size:       1064     138    2288     374     146    1280

  Fragments arranged by size: 

                2288    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2069    2192    3353    3950    4737    5005    1239 
   Size:        393     437     123    1161     597     787     268    1524

  Fragments arranged by size: 

                1524    1161     787     597     437     393     268     123
            
 
EcoRI G'AATT_C

Cuts at:   2039    2599    2977    2039 
   Size:        560     378    4352

  Fragments arranged by size: 

                4352     560     378
 
FspI TGC'GCA

Cuts at:   3796    3796 
   Size:       5290
 
HaeII r_GCGC'y

Cuts at:   1678    4437    1678 
   Size:       2759    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2109    2141    2152    2184    3931 
   Size:         32     135      32     537      32      11      32    1747

Cuts at:   3931    3963    4005    4037    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1747     537     135      42      32      32      32
                  32      32      32      11
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2634    2880     669 
   Size:        988     281     696     246    3079

  Fragments arranged by size: 

                3079     988     696     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2544     748 
   Size:       1796    3494
 
HpaI GTT'AAC

Cuts at:   2880    2880 
   Size:       5290
 
KpnI G_GTAC'C

Cuts at:   2615    2615 
   Size:       5290
 
MfeI C'AATT_G

Cuts at:   2889    2889 
   Size:       5290
 
MluI A'CGCG_T

Cuts at:   5166    5166 
   Size:       5290
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4404    4588    1305 
   Size:       3099     184    2007

  Fragments arranged by size: 

                3099    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2033      10 
   Size:         54    1425     343     201    3267

  Fragments arranged by size: 

                3267    1425     343     201      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3248    3607    3766     518 
   Size:        648    2082     359     159    2042

  Fragments arranged by size: 

                2082    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2706    3383    4531    4776    1606 
   Size:       1100     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1100     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3799

  Fragments arranged by size: 

                3799     939     552
 
NdeI CA'TA_TG

Cuts at:    387    2452     387 
   Size:       2065    3225
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5290
 
NotI GC'GGCC_GC

Cuts at:   2737    2737 
   Size:       5290
 
NspI r_CATG'y

Cuts at:   1132    1229    2648    4193    1132 
   Size:         97    1419    1545    2229

  Fragments arranged by size: 

                2229    1545    1419      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5290
 
PciI A'CATG_T

Cuts at:   4189    4189 
   Size:       5290
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5290
 
PmeI GTTT'AAAC

Cuts at:   2594    2594 
   Size:       5290
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3359    3391    4872    4904    3359 
   Size:         32    1481      32    3745

  Fragments arranged by size: 

                3745    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    2259    1613 
   Size:        646    4644
 
PsiI TTA'TAA

Cuts at:   2394    2860    2394 
   Size:        466    4824
 
PstI C_TGCA'G

Cuts at:    830    2642     830 
   Size:       1812    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5290
 
SalI G'TCGA_C

Cuts at:   1936    2632    1936 
   Size:        696    4594
 
SbfI CC_TGCA'GG

Cuts at:   2642    2642 
   Size:       5290
 
ScaI AGT'ACT

Cuts at:   3538    5250    3538 
   Size:       1712    3578
 
SexAI A'CCwGG_T

Cuts at:   2121    2121 
   Size:       5290
 
SfcI C'TryA_G

Cuts at:    826    1045    2113    2638    3773    4454    4645     826 
   Size:        219    1068     525    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1068     681     525     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5290
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5290
 
SmaI CCC'GGG

Cuts at:   1872    2617    1872 
   Size:        745    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3394    4295    4557     820 
   Size:        197     926    1451     901     262    1553

  Fragments arranged by size: 

                1553    1451     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3081     493 
   Size:       2588    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5290
 
SphI G_CATG'C

Cuts at:   1132    2648    1132 
   Size:       1516    3774
 
SspI AAT'ATT

Cuts at:      5      52    3214       5 
   Size:         47    3162    2081

  Fragments arranged by size: 

                3162    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5290
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2131    2729     513 
   Size:        552     939     127     598    3074

  Fragments arranged by size: 

                3074     939     598     552     127
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3317    3334    3487    3672    4117     325 
   Size:       1255    1737      17     153     185     445    1498

  Fragments arranged by size: 

                1737    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2377    3536    5248 
   Size:        274      80      33      51    1443     400    1159    1712

Cuts at:   5248      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1159     400     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5250    5282    5250 
   Size:         32    5258
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5290
 
XbaI T'CTAG_A

Cuts at:   2626    2626 
   Size:       5290
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2011    2011 
   Size:       5290
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5290
 
XmaI C'CCGG_G

Cuts at:   1870    2615    1870 
   Size:        745    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3419    3419 
   Size:       5290
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3098     276 
   Size:         53      83     186     936    1564    2468

  Fragments arranged by size: 

                2468    1564     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PstI     PvuI     SalI     SbfI     ScaI    SexAI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI
    SacII    SanDI     SapI     SfiI     SfoI     SrfI     SwaI
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