(Circular) (Six-base) MAPSORT of: pFN21AB9637.seq Check: 2740 from: 1 to: 5938 pFN21AB9637 5938 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3748 278 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3281 1937 Size: 1344 4594 Acc65I G'GTAC_C Cuts at: 3259 3259 Size: 5938 AclI AA'CG_TT Cuts at: 1563 4065 4438 1563 Size: 2502 373 3063 Fragments arranged by size: 3063 2502 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2570 4001 5385 5653 1632 Size: 938 1431 1384 268 1917 Fragments arranged by size: 1917 1431 1384 938 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5741 AflIII A'CryG_T Cuts at: 1203 4837 5814 1203 Size: 3634 977 1327 Fragments arranged by size: 3634 1327 977 AgeI A'CCGG_T Cuts at: 4769 4769 Size: 5938 AhdI GACnn_n'nnGTC Cuts at: 4667 4667 Size: 5938 AleI CACnn'nnGTG Cuts at: 1166 2037 2246 1166 Size: 871 209 4858 Fragments arranged by size: 4858 871 209 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2264 1184 Size: 1080 4858 AlwNI CAG_nnn'CTG Cuts at: 2479 2680 2954 3681 5253 2479 Size: 201 274 727 1572 3164 Fragments arranged by size: 3164 1572 727 274 201 ApaLI G'TGCA_C Cuts at: 3995 5151 3995 Size: 1156 4782 ApoI r'AATT_y Cuts at: 1493 3247 3473 3625 5734 1493 Size: 1754 226 152 2109 1697 Fragments arranged by size: 2109 1754 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4492 160 Size: 872 3460 1606 Fragments arranged by size: 3460 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 AvaI C'yCGr_G Cuts at: 1870 1943 3263 1870 Size: 73 1320 4545 Fragments arranged by size: 4545 1320 73 BamHI G'GATC_C Cuts at: 3268 3635 3268 Size: 367 5571 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2172 3259 4714 618 Size: 325 217 627 385 1087 1455 1842 Fragments arranged by size: 1842 1455 1087 627 385 325 217 BanII G_rGCy'C Cuts at: 1391 3257 1391 Size: 1866 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2060 2468 928 Size: 971 161 408 4398 Fragments arranged by size: 4398 971 408 161 BbvCI CC'TCA_GC Cuts at: 2622 2622 Size: 5938 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4129 4163 1903 Size: 34 2192 34 3678 Fragments arranged by size: 3678 2192 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2725 3830 5040 2725 Size: 1105 1210 3623 Fragments arranged by size: 3623 1210 1105 BclI T'GATC_A Cuts at: 1548 1548 Size: 5938 BfrBI ATG'CAT Cuts at: 3230 3230 Size: 5938 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4549 136 Size: 107 122 71 631 605 2877 1525 Fragments arranged by size: 2877 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5933 5933 Size: 5938 BlpI GC'TnA_GC Cuts at: 3355 3355 Size: 5938 Bme1580I G_kGCm'C Cuts at: 1790 2227 2419 3140 3999 5155 1790 Size: 437 192 721 859 1156 2573 Fragments arranged by size: 2573 1156 859 721 437 192 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5938 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3669 4627 442 Size: 416 53 83 399 24 2252 958 1753 Fragments arranged by size: 2252 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5938 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2249 4598 1239 Size: 836 174 2349 2579 Fragments arranged by size: 2579 2349 836 174 Bpu10I CC'TnA_GC Cuts at: 2622 2904 2622 Size: 282 5656 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4063 4928 5226 4063 Size: 865 298 4775 Fragments arranged by size: 4775 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2499 4601 882 Size: 617 1000 2102 2219 Fragments arranged by size: 2219 2102 1000 617 BsaAI yAC'GTr Cuts at: 493 1204 3729 493 Size: 711 2525 2702 Fragments arranged by size: 2702 2525 711 BsaBI GATnn'nnATC Cuts at: 5932 5932 Size: 5938 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2365 3745 4127 4762 Size: 53 83 186 936 832 1380 382 635 Cuts at: 4762 275 Size: 1451 Fragments arranged by size: 1451 1380 936 832 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2064 2501 4371 4769 5043 5190 5601 2064 Size: 437 1870 398 274 147 411 2401 Fragments arranged by size: 2401 1870 437 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2002 2032 1178 Size: 30 165 30 599 30 5084 Fragments arranged by size: 5084 599 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 2718 1186 Size: 958 574 4406 Fragments arranged by size: 4406 958 574 BseYI C'CCAG_C Cuts at: 2158 2664 5141 5637 2158 Size: 506 2477 496 2459 Fragments arranged by size: 2477 2459 506 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5365 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3388 4149 5177 1540 Size: 275 186 1387 761 1028 2301 Fragments arranged by size: 2301 1387 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2044 2243 2519 3999 4084 5155 2044 Size: 199 276 1480 85 1071 2827 Fragments arranged by size: 2827 1480 1071 276 199 85 BsmI GAATG_Cn' Cuts at: 3230 3449 3542 3230 Size: 219 93 5626 Fragments arranged by size: 5626 219 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2227 2243 2419 2519 3140 3257 Size: 399 254 183 16 176 100 621 117 Cuts at: 3257 3999 4084 5155 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 621 399 254 183 176 117 100 85 16 BspEI T'CCGG_A Cuts at: 5601 5601 Size: 5938 BspHI T'CATG_A Cuts at: 3827 5557 3827 Size: 1730 4208 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2937 2980 3293 844 Size: 2093 43 313 3489 Fragments arranged by size: 3489 2093 313 43 BsrBI CCG'CTC Cuts at: 1394 1823 3825 1394 Size: 429 2002 3507 Fragments arranged by size: 3507 2002 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4433 4607 65 Size: 1183 3185 174 1396 Fragments arranged by size: 3185 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4582 4769 1700 Size: 110 2772 187 2869 Fragments arranged by size: 2869 2772 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5938 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5938 BssSI C'ACGA_G Cuts at: 3998 5010 3998 Size: 1012 4926 BstAPI GCAn_nnn'nTGC Cuts at: 794 2680 794 Size: 1886 4052 BstBI TT'CG_AA Cuts at: 4742 4742 Size: 5938 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5938 BstXI CCAn_nnnn'nTGG Cuts at: 2113 2426 2113 Size: 313 5625 BstYI r'GATC_y Cuts at: 1972 3268 3635 4020 4037 5489 5575 5587 5933 Size: 1296 367 385 17 1452 86 12 346 Cuts at: 5933 1972 Size: 1977 Fragments arranged by size: 1977 1452 1296 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3132 513 Size: 552 939 1128 3319 Fragments arranged by size: 3319 1128 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5199 Fragments arranged by size: 5199 683 56 BtsI GCAGTG_nn' Cuts at: 2203 3454 4248 4268 2203 Size: 1251 794 20 3873 Fragments arranged by size: 3873 1251 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5903 DraI TTT'AAA Cuts at: 3242 3242 Size: 5938 DraIII CAC_nnn'GTG Cuts at: 2374 2374 Size: 5938 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4945 809 Size: 507 3629 1802 Fragments arranged by size: 3629 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 3385 4274 Size: 54 1425 50 275 18 360 1195 889 Cuts at: 4274 5903 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1195 889 360 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3385 1537 Size: 275 1573 4090 Fragments arranged by size: 4090 1573 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2274 3868 1353 Size: 921 1594 3423 Fragments arranged by size: 3423 1594 921 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2721 4525 4899 5045 387 Size: 1064 138 1132 1804 374 146 1280 Fragments arranged by size: 1804 1280 1132 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2249 2570 4001 4598 5385 5653 Size: 393 443 174 321 1431 597 787 268 Cuts at: 5653 1239 Size: 1524 Fragments arranged by size: 1524 1431 787 597 443 393 321 268 174 EcoNI CCTnn'n_nnAGG Cuts at: 2933 2933 Size: 5938 EcoO109I rG'GnC_Cy Cuts at: 2203 3065 2203 Size: 862 5076 EcoRI G'AATT_C Cuts at: 3247 3625 3247 Size: 378 5560 FspI TGC'GCA Cuts at: 4444 4444 Size: 5938 HaeII r_GCGC'y Cuts at: 1678 5085 1678 Size: 3407 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2852 2884 4579 4611 4653 Size: 32 135 32 1280 32 1695 32 42 Cuts at: 4653 4685 1373 Size: 32 2626 Fragments arranged by size: 2626 1695 1280 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3282 3528 669 Size: 988 281 1344 246 3079 Fragments arranged by size: 3079 1344 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5938 HpaI GTT'AAC Cuts at: 3528 3528 Size: 5938 KpnI G_GTAC'C Cuts at: 3263 3263 Size: 5938 MfeI C'AATT_G Cuts at: 3537 3537 Size: 5938 MluI A'CGCG_T Cuts at: 5814 5814 Size: 5938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2569 5052 5236 1305 Size: 1264 2483 184 2007 Fragments arranged by size: 2483 2007 1264 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4116 Fragments arranged by size: 4116 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2037 2246 3896 4255 4414 518 Size: 648 871 209 1650 359 159 2042 Fragments arranged by size: 2042 1650 871 648 359 209 159 MspA1I CmG'CkG Cuts at: 1606 2621 2680 3013 3191 3354 4031 5179 5424 Size: 1015 59 333 178 163 677 1148 245 Cuts at: 5424 1606 Size: 2120 Fragments arranged by size: 2120 1148 1015 677 333 245 178 163 59 NcoI C'CATG_G Cuts at: 513 1065 2004 3132 513 Size: 552 939 1128 3319 Fragments arranged by size: 3319 1128 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5938 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5938 NotI GC'GGCC_GC Cuts at: 3385 3385 Size: 5938 NsiI A_TGCA'T Cuts at: 3232 3232 Size: 5938 NspI r_CATG'y Cuts at: 1132 1229 3296 4841 1132 Size: 97 2067 1545 2229 Fragments arranged by size: 2229 2067 1545 97 PasI CC'CwG_GG Cuts at: 1348 3061 1348 Size: 1713 4225 PciI A'CATG_T Cuts at: 4837 4837 Size: 5938 PflMI CCAn_nnn'nTGG Cuts at: 1108 2374 1108 Size: 1266 4672 PmeI GTTT'AAAC Cuts at: 3242 3242 Size: 5938 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2042 2074 4007 4039 5520 5552 2042 Size: 32 1933 32 1481 32 2428 Fragments arranged by size: 2428 1933 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2203 2203 Size: 5938 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5938 PsiI TTA'TAA Cuts at: 3508 3508 Size: 5938 PstI C_TGCA'G Cuts at: 830 2950 3290 830 Size: 2120 340 3478 Fragments arranged by size: 3478 2120 340 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5938 PvuII CAG'CTG Cuts at: 2621 3013 3191 2621 Size: 392 178 5368 Fragments arranged by size: 5368 392 178 SalI G'TCGA_C Cuts at: 1936 3280 1936 Size: 1344 4594 SbfI CC_TGCA'GG Cuts at: 3290 3290 Size: 5938 ScaI AGT'ACT Cuts at: 4186 5898 4186 Size: 1712 4226 SexAI A'CCwGG_T Cuts at: 2370 2370 Size: 5938 SfcI C'TryA_G Cuts at: 826 1045 2946 3286 4421 5102 5293 826 Size: 219 1901 340 1135 681 191 1471 Fragments arranged by size: 1901 1471 1135 681 340 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5938 SmaI CCC'GGG Cuts at: 1872 3265 1872 Size: 1393 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4042 4943 5205 820 Size: 197 926 2099 901 262 1553 Fragments arranged by size: 2099 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3729 493 Size: 3236 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5938 SphI G_CATG'C Cuts at: 1132 3296 1132 Size: 2164 3774 SspI AAT'ATT Cuts at: 5 52 3862 5 Size: 47 3810 2081 Fragments arranged by size: 3810 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5938 StyI C'CwwG_G Cuts at: 513 1065 2004 2301 2934 3132 3377 513 Size: 552 939 297 633 198 245 3074 Fragments arranged by size: 3074 939 633 552 297 245 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2112 3965 3982 4135 4320 4765 325 Size: 1255 532 1853 17 153 185 445 1498 Fragments arranged by size: 1853 1498 1255 532 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 2754 4184 Size: 274 80 33 51 1443 168 609 1430 Cuts at: 4184 5896 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1430 609 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2387 2419 2629 2661 5898 5930 2387 Size: 32 210 32 3237 32 2395 Fragments arranged by size: 3237 2395 210 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5938 XbaI T'CTAG_A Cuts at: 3274 3274 Size: 5938 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3038 3038 Size: 5938 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5938 XmaI C'CCGG_G Cuts at: 1870 3263 1870 Size: 1393 4545 XmnI GAAnn'nnTTC Cuts at: 2057 4067 2057 Size: 2010 3928 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3746 276 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI