Restriction map for
pFN21AB9637
(Linear) (Six-base) MAPSORT of: KIBB9637.seq Check: 2495 from: 1 to: 1233
KIBB9637.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1233
Size: 565 668
AleI CACnn'nnGTG
Cuts at: 0 32 241 1233
Size: 32 209 992
Fragments arranged by size:
992 209 32
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 259 1233
Size: 259 974
AlwNI CAG_nnn'CTG
Cuts at: 0 474 675 949 1233
Size: 474 201 274 284
Fragments arranged by size:
474 284 274 201
BanI G'GyrC_C
Cuts at: 0 167 1233
Size: 167 1066
BbsI GAAGACnn'nnnn_
Cuts at: 0 55 463 1233
Size: 55 408 770
Fragments arranged by size:
770 408 55
BbvCI CC'TCA_GC
Cuts at: 0 617 1233
Size: 617 616
BciVI GTATCCnnnnn_n'
Cuts at: 0 720 1233
Size: 720 513
BfrBI ATG'CAT
Cuts at: 0 1225 1233
Size: 1225 8
Bme1580I G_kGCm'C
Cuts at: 0 222 414 1135 1233
Size: 222 192 721 98
Fragments arranged by size:
721 222 192 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 244 1233
Size: 70 174 989
Fragments arranged by size:
989 174 70
Bpu10I CC'TnA_GC
Cuts at: 0 617 899 1233
Size: 617 282 334
Fragments arranged by size:
617 334 282
BsaI GGTCTCn'nnnn_
Cuts at: 0 494 1233
Size: 494 739
BsaHI Gr'CG_yC
Cuts at: 0 360 1233
Size: 360 873
BsaWI w'CCGG_w
Cuts at: 0 59 496 1233
Size: 59 437 737
Fragments arranged by size:
737 437 59
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 27 1233
Size: 27 1206
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 713 1233
Size: 139 574 520
Fragments arranged by size:
574 520 139
BseYI C'CCAG_C
Cuts at: 0 153 659 1233
Size: 153 506 574
Fragments arranged by size:
574 506 153
BsiHKAI G_wGCw'C
Cuts at: 0 39 238 514 1233
Size: 39 199 276 719
Fragments arranged by size:
719 276 199 39
BsmI GAATG_Cn'
Cuts at: 0 1225 1233
Size: 1225 8
Bsp1286I G_dGCh'C
Cuts at: 0 39 222 238 414 514 1135 1233
Size: 39 183 16 176 100 621 98
Fragments arranged by size:
621 183 176 100 98 39 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 932 975 1233
Size: 932 43 258
Fragments arranged by size:
932 258 43
BstAPI GCAn_nnn'nTGC
Cuts at: 0 675 1233
Size: 675 558
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 421 1233
Size: 108 313 812
Fragments arranged by size:
812 313 108
BtgI C'CryG_G
Cuts at: 0 1127 1233
Size: 1127 106
BtsI GCAGTG_nn'
Cuts at: 0 198 1233
Size: 198 1035
DraIII CAC_nnn'GTG
Cuts at: 0 369 1233
Size: 369 864
EaeI y'GGCC_r
Cuts at: 0 185 1233
Size: 185 1048
EarI CTCTTCn'nnn_
Cuts at: 0 269 1233
Size: 269 964
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 716 1233
Size: 716 517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 244 565 1233
Size: 70 174 321 668
Fragments arranged by size:
668 321 174 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 928 1233
Size: 928 305
EcoO109I rG'GnC_Cy
Cuts at: 0 198 1060 1233
Size: 198 862 173
Fragments arranged by size:
862 198 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 847 879 1233
Size: 847 32 354
Fragments arranged by size:
847 354 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 1233
Size: 564 669
MslI CAynn'nnrTG
Cuts at: 0 32 241 1233
Size: 32 209 992
Fragments arranged by size:
992 209 32
MspA1I CmG'CkG
Cuts at: 0 616 675 1008 1186 1233
Size: 616 59 333 178 47
Fragments arranged by size:
616 333 178 59 47
NcoI C'CATG_G
Cuts at: 0 1127 1233
Size: 1127 106
NsiI A_TGCA'T
Cuts at: 0 1227 1233
Size: 1227 6
PasI CC'CwG_GG
Cuts at: 0 1056 1233
Size: 1056 177
PflMI CCAn_nnn'nTGG
Cuts at: 0 369 1233
Size: 369 864
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 1233
Size: 37 32 1164
Fragments arranged by size:
1164 37 32
PpuMI rG'GwC_Cy
Cuts at: 0 198 1233
Size: 198 1035
PstI C_TGCA'G
Cuts at: 0 945 1233
Size: 945 288
PvuII CAG'CTG
Cuts at: 0 616 1008 1186 1233
Size: 616 392 178 47
Fragments arranged by size:
616 392 178 47
SexAI A'CCwGG_T
Cuts at: 0 365 1233
Size: 365 868
SfcI C'TryA_G
Cuts at: 0 941 1233
Size: 941 292
StyI C'CwwG_G
Cuts at: 0 296 929 1127 1233
Size: 296 633 198 106
Fragments arranged by size:
633 296 198 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 107 1233
Size: 107 1126
TatI w'GTAC_w
Cuts at: 0 140 749 1233
Size: 140 609 484
Fragments arranged by size:
609 484 140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 382 414 624 656 1233
Size: 382 32 210 32 577
Fragments arranged by size:
577 382 210 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1033 1233
Size: 1033 200
XmnI GAAnn'nnTTC
Cuts at: 0 52 1233
Size: 52 1181
Enzymes that do cut:
AcuI AleI AlfI AlwNI BanI BbsI BbvCI BciVI
BfrBI Bme1580I BpmI Bpu10I BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BstAPI BstXI
BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I Hin4I MmeI MslI MspA1I NcoI NsiI PasI
PflMI PpiI PpuMI PstI PvuII SexAI SfcI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BcgI
BclI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaAI BsaBI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NspI PacI PciI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmaI ZraI