(Circular) (Six-base) MAPSORT of: pFN21AB9634.seq Check: 4086 from: 1 to: 5680 pFN21AB9634 5680 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3490 278 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3023 1937 Size: 1086 4594 Acc65I G'GTAC_C Cuts at: 2881 3001 2881 Size: 120 5560 AclI AA'CG_TT Cuts at: 1563 3807 4180 1563 Size: 2244 373 3063 Fragments arranged by size: 3063 2244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2146 2563 2645 3743 5127 5395 1632 Size: 514 417 82 1098 1384 268 1917 Fragments arranged by size: 1917 1384 1098 514 417 268 82 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5483 AflIII A'CryG_T Cuts at: 1203 2852 4579 5556 1203 Size: 1649 1727 977 1327 Fragments arranged by size: 1727 1649 1327 977 AgeI A'CCGG_T Cuts at: 4511 4511 Size: 5680 AhdI GACnn_n'nnGTC Cuts at: 2952 4409 2952 Size: 1457 4223 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5680 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5680 AlwNI CAG_nnn'CTG Cuts at: 2409 3423 4995 2409 Size: 1014 1572 3094 Fragments arranged by size: 3094 1572 1014 ApaI G_GGCC'C Cuts at: 2553 2782 2553 Size: 229 5451 ApaLI G'TGCA_C Cuts at: 3737 4893 3737 Size: 1156 4524 ApoI r'AATT_y Cuts at: 1493 2989 3215 3367 5476 1493 Size: 1496 226 152 2109 1697 Fragments arranged by size: 2109 1697 1496 226 152 AseI AT'TA_AT Cuts at: 160 1032 4234 160 Size: 872 3202 1606 Fragments arranged by size: 3202 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 AvaI C'yCGr_G Cuts at: 1870 1943 2123 2731 3005 1870 Size: 73 180 608 274 4545 Fragments arranged by size: 4545 608 274 180 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2736 2769 2736 Size: 33 5647 BamHI G'GATC_C Cuts at: 3010 3377 3010 Size: 367 5313 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2704 2881 3001 4456 618 Size: 325 217 627 917 177 120 1455 1842 Fragments arranged by size: 1842 1455 917 627 325 217 177 120 BanII G_rGCy'C Cuts at: 1391 2553 2621 2732 2782 2999 1391 Size: 1162 68 111 50 217 4072 Fragments arranged by size: 4072 1162 217 111 68 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4709 BbvCI CC'TCA_GC Cuts at: 2242 2278 2242 Size: 36 5644 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3871 3905 1903 Size: 34 50 34 1850 34 3678 Fragments arranged by size: 3678 1850 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2024 2730 2737 3572 4782 2024 Size: 706 7 835 1210 2922 Fragments arranged by size: 2922 1210 835 706 7 BclI T'GATC_A Cuts at: 1548 1548 Size: 5680 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4291 136 Size: 107 122 71 631 605 2619 1525 Fragments arranged by size: 2619 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5675 5675 Size: 5680 BlpI GC'TnA_GC Cuts at: 3097 3097 Size: 5680 Bme1580I G_kGCm'C Cuts at: 1790 2553 2782 3741 4897 1790 Size: 763 229 959 1156 2573 Fragments arranged by size: 2573 1156 959 763 229 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5680 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2354 3411 4369 Size: 416 53 83 399 24 937 1057 958 Cuts at: 4369 442 Size: 1753 Fragments arranged by size: 1753 1057 958 937 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2365 2547 4340 1239 Size: 1126 182 1793 2579 Fragments arranged by size: 2579 1793 1126 182 Bpu10I CC'TnA_GC Cuts at: 2242 2278 2351 2242 Size: 36 73 5571 Fragments arranged by size: 5571 73 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2744 3805 4670 4968 2744 Size: 1061 865 298 3456 Fragments arranged by size: 3456 1061 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2960 4343 882 Size: 617 1461 1383 2219 Fragments arranged by size: 2219 1461 1383 617 BsaAI yAC'GTr Cuts at: 493 1204 3471 493 Size: 711 2267 2702 Fragments arranged by size: 2702 2267 711 BsaBI GATnn'nnATC Cuts at: 5674 5674 Size: 5680 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3487 3869 4504 275 Size: 53 83 186 936 1954 382 635 1451 Fragments arranged by size: 1954 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4113 4511 4785 4932 5343 4113 Size: 398 274 147 411 4450 Fragments arranged by size: 4450 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5455 Fragments arranged by size: 5455 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2230 2239 2242 2251 2961 1186 Size: 1044 9 3 9 710 3905 Fragments arranged by size: 3905 1044 710 9 9 3 BseYI C'CCAG_C Cuts at: 2156 2435 2451 2492 2597 2603 2656 4883 5379 Size: 279 16 41 105 6 53 2227 496 Cuts at: 5379 2156 Size: 2457 Fragments arranged by size: 2457 2227 496 279 105 53 41 16 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5107 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3130 3891 4919 1540 Size: 275 186 1129 761 1028 2301 Fragments arranged by size: 2301 1129 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3741 3826 4897 3741 Size: 85 1071 4524 Fragments arranged by size: 4524 1071 85 BsmI GAATG_Cn' Cuts at: 3191 3284 3191 Size: 93 5587 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2553 2621 2732 2782 2999 3741 3826 Size: 399 763 68 111 50 217 742 85 Cuts at: 3826 4897 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 763 742 399 217 111 85 68 50 BspEI T'CCGG_A Cuts at: 5343 5343 Size: 5680 BspHI T'CATG_A Cuts at: 3569 5299 3569 Size: 1730 3950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3035 844 Size: 2191 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2355 2775 3567 1394 Size: 429 532 420 792 3507 Fragments arranged by size: 3507 792 532 429 420 BsrDI GCAATG_nn' Cuts at: 65 1248 4175 4349 65 Size: 1183 2927 174 1396 Fragments arranged by size: 2927 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2591 4324 4511 1700 Size: 110 781 1733 187 2869 Fragments arranged by size: 2869 1733 781 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5680 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5680 BssSI C'ACGA_G Cuts at: 3740 4752 3740 Size: 1012 4668 BstAPI GCAn_nnn'nTGC Cuts at: 794 2409 794 Size: 1615 4065 BstBI TT'CG_AA Cuts at: 4484 4484 Size: 5680 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5680 BstXI CCAn_nnnn'nTGG Cuts at: 2113 2840 2113 Size: 727 4953 BstYI r'GATC_y Cuts at: 1972 3010 3377 3762 3779 5231 5317 5329 5675 Size: 1038 367 385 17 1452 86 12 346 Cuts at: 5675 1972 Size: 1977 Fragments arranged by size: 1977 1452 1038 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2106 513 Size: 552 1041 4087 Fragments arranged by size: 4087 1041 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4941 Fragments arranged by size: 4941 683 56 BtsI GCAGTG_nn' Cuts at: 2145 3196 3990 4010 2145 Size: 1051 794 20 3815 Fragments arranged by size: 3815 1051 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5645 DraI TTT'AAA Cuts at: 2984 2984 Size: 5680 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4687 809 Size: 507 3371 1802 Fragments arranged by size: 3371 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 2479 3127 Size: 54 1425 50 275 18 285 364 648 Cuts at: 3127 4016 5645 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 648 364 285 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3127 1537 Size: 275 1315 4090 Fragments arranged by size: 4090 1315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3610 1353 Size: 2257 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4267 4641 4787 387 Size: 1064 138 2678 374 146 1280 Fragments arranged by size: 2678 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2146 2365 2547 2563 2645 3743 4340 Size: 393 514 219 182 16 82 1098 597 Cuts at: 4340 5127 5395 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1098 787 597 514 393 268 219 182 82 16 EcoO109I rG'GnC_Cy Cuts at: 2051 2371 2550 2778 2779 2902 2946 2051 Size: 320 179 228 1 123 44 4785 Fragments arranged by size: 4785 320 228 179 123 44 1 EcoRI G'AATT_C Cuts at: 2989 3367 2989 Size: 378 5302 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2890 2890 Size: 5680 FspI TGC'GCA Cuts at: 4186 4186 Size: 5680 HaeII r_GCGC'y Cuts at: 1678 4827 1678 Size: 3149 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2360 2392 2938 2970 4321 Size: 32 135 32 788 32 546 32 1351 Cuts at: 4321 4353 4395 4427 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1351 788 546 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3024 3270 669 Size: 988 281 1086 246 3079 Fragments arranged by size: 3079 1086 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5680 HpaI GTT'AAC Cuts at: 3270 3270 Size: 5680 KpnI G_GTAC'C Cuts at: 2885 3005 2885 Size: 120 5560 MfeI C'AATT_G Cuts at: 3279 3279 Size: 5680 MluI A'CGCG_T Cuts at: 5556 5556 Size: 5680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2892 4794 4978 1305 Size: 1587 1902 184 2007 Fragments arranged by size: 2007 1902 1587 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 10 Size: 54 1425 343 285 3573 Fragments arranged by size: 3573 1425 343 285 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 2841 3638 3997 4156 518 Size: 648 1005 670 797 359 159 2042 Fragments arranged by size: 2042 1005 797 670 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2138 2215 2533 2656 2802 3096 3773 4921 Size: 532 77 318 123 146 294 677 1148 Cuts at: 4921 5166 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 532 318 294 245 146 123 77 NcoI C'CATG_G Cuts at: 513 1065 2106 513 Size: 552 1041 4087 Fragments arranged by size: 4087 1041 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5680 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5680 NotI GC'GGCC_GC Cuts at: 3127 3127 Size: 5680 NspI r_CATG'y Cuts at: 1132 1229 2856 3038 4583 1132 Size: 97 1627 182 1545 2229 Fragments arranged by size: 2229 1627 1545 182 97 PasI CC'CwG_GG Cuts at: 1348 2463 2962 1348 Size: 1115 499 4066 Fragments arranged by size: 4066 1115 499 PciI A'CATG_T Cuts at: 2852 4579 2852 Size: 1727 3953 PflMI CCAn_nnn'nTGG Cuts at: 1108 2656 1108 Size: 1548 4132 PfoI T'CCnGG_A Cuts at: 2047 2047 Size: 5680 PmeI GTTT'AAAC Cuts at: 2984 2984 Size: 5680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3749 3781 5262 5294 3749 Size: 32 1481 32 4135 Fragments arranged by size: 4135 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2051 2946 2051 Size: 895 4785 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5680 PsiI TTA'TAA Cuts at: 3250 3250 Size: 5680 PspOMI G'GGCC_C Cuts at: 2549 2778 2549 Size: 229 5451 PstI C_TGCA'G Cuts at: 830 3032 830 Size: 2202 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5680 PvuII CAG'CTG Cuts at: 2215 2533 2656 2215 Size: 318 123 5239 Fragments arranged by size: 5239 318 123 SalI G'TCGA_C Cuts at: 1936 3022 1936 Size: 1086 4594 SanDI GG'GwC_CC Cuts at: 2051 2051 Size: 5680 SbfI CC_TGCA'GG Cuts at: 3032 3032 Size: 5680 ScaI AGT'ACT Cuts at: 3928 5640 3928 Size: 1712 3968 SfcI C'TryA_G Cuts at: 826 1045 3028 4163 4844 5035 826 Size: 219 1983 1135 681 191 1471 Fragments arranged by size: 1983 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5680 SmaI CCC'GGG Cuts at: 1872 3007 1872 Size: 1135 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2723 3784 4685 4947 820 Size: 197 926 780 1061 901 262 1553 Fragments arranged by size: 1553 1061 926 901 780 262 197 SnaBI TAC'GTA Cuts at: 493 3471 493 Size: 2978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5680 SphI G_CATG'C Cuts at: 1132 3038 1132 Size: 1906 3774 SspI AAT'ATT Cuts at: 5 52 3604 5 Size: 47 3552 2081 Fragments arranged by size: 3552 2081 47 StuI AGG'CCT Cuts at: 1517 2511 1517 Size: 994 4686 StyI C'CwwG_G Cuts at: 513 1065 2106 2763 3119 513 Size: 552 1041 657 356 3074 Fragments arranged by size: 3074 1041 657 552 356 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2663 2722 3707 3724 3877 4062 4507 Size: 1255 1083 59 985 17 153 185 445 Cuts at: 4507 325 Size: 1498 Fragments arranged by size: 1498 1255 1083 985 445 185 153 59 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3926 5638 96 Size: 274 80 33 51 1443 1949 1712 138 Fragments arranged by size: 1949 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5640 5672 5640 Size: 32 5648 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5680 XbaI T'CTAG_A Cuts at: 3016 3016 Size: 5680 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2024 2110 2605 2653 2024 Size: 86 495 48 5051 Fragments arranged by size: 5051 495 86 48 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5680 XmaI C'CCGG_G Cuts at: 1870 3005 1870 Size: 1135 4545 XmnI GAAnn'nnTTC Cuts at: 2630 3809 2630 Size: 1179 4501 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3488 276 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI