Restriction map for pFN21AB9634
 (Circular) (Six-base) MAPSORT of: pFN21AB9634.seq  Check: 4086  from: 1  to: 5680



pFN21AB9634  5680 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:28  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3490     278 
   Size:         53      83     186     936    1954    2468

  Fragments arranged by size: 

                2468    1954     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3023    1937 
   Size:       1086    4594
 
Acc65I G'GTAC_C

Cuts at:   2881    3001    2881 
   Size:        120    5560
 
AclI AA'CG_TT

Cuts at:   1563    3807    4180    1563 
   Size:       2244     373    3063

  Fragments arranged by size: 

                3063    2244     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2146    2563    2645    3743    5127    5395    1632 
   Size:        514     417      82    1098    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1098     514     417     268      82
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5483
 
AflIII A'CryG_T

Cuts at:   1203    2852    4579    5556    1203 
   Size:       1649    1727     977    1327

  Fragments arranged by size: 

                1727    1649    1327     977
 
AgeI A'CCGG_T

Cuts at:   4511    4511 
   Size:       5680
 
AhdI GACnn_n'nnGTC

Cuts at:   2952    4409    2952 
   Size:       1457    4223
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5680
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5680
 
AlwNI CAG_nnn'CTG

Cuts at:   2409    3423    4995    2409 
   Size:       1014    1572    3094

  Fragments arranged by size: 

                3094    1572    1014
 
ApaI G_GGCC'C

Cuts at:   2553    2782    2553 
   Size:        229    5451
 
ApaLI G'TGCA_C

Cuts at:   3737    4893    3737 
   Size:       1156    4524
 
ApoI r'AATT_y

Cuts at:   1493    2989    3215    3367    5476    1493 
   Size:       1496     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1496     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4234     160 
   Size:        872    3202    1606

  Fragments arranged by size: 

                3202    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5680
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2123    2731    3005    1870 
   Size:         73     180     608     274    4545

  Fragments arranged by size: 

                4545     608     274     180      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2736    2769    2736 
   Size:         33    5647
 
BamHI G'GATC_C

Cuts at:   3010    3377    3010 
   Size:        367    5313
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2704    2881    3001    4456     618 
   Size:        325     217     627     917     177     120    1455    1842

  Fragments arranged by size: 

                1842    1455     917     627     325     217     177     120
            
 
BanII G_rGCy'C

Cuts at:   1391    2553    2621    2732    2782    2999    1391 
   Size:       1162      68     111      50     217    4072

  Fragments arranged by size: 

                4072    1162     217     111      68      50
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4709
 
BbvCI CC'TCA_GC

Cuts at:   2242    2278    2242 
   Size:         36    5644
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3871    3905    1903 
   Size:         34      50      34    1850      34    3678

  Fragments arranged by size: 

                3678    1850      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2024    2730    2737    3572    4782    2024 
   Size:        706       7     835    1210    2922

  Fragments arranged by size: 

                2922    1210     835     706       7
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5680
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4291     136 
   Size:        107     122      71     631     605    2619    1525

  Fragments arranged by size: 

                2619    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5675    5675 
   Size:       5680
 
BlpI GC'TnA_GC

Cuts at:   3097    3097 
   Size:       5680
 
Bme1580I G_kGCm'C

Cuts at:   1790    2553    2782    3741    4897    1790 
   Size:        763     229     959    1156    2573

  Fragments arranged by size: 

                2573    1156     959     763     229
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5680
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2354    3411    4369 
   Size:        416      53      83     399      24     937    1057     958

Cuts at:   4369     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1057     958     937     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5680
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2365    2547    4340    1239 
   Size:       1126     182    1793    2579

  Fragments arranged by size: 

                2579    1793    1126     182
 
Bpu10I CC'TnA_GC

Cuts at:   2242    2278    2351    2242 
   Size:         36      73    5571

  Fragments arranged by size: 

                5571      73      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2744    3805    4670    4968    2744 
   Size:       1061     865     298    3456

  Fragments arranged by size: 

                3456    1061     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2960    4343     882 
   Size:        617    1461    1383    2219

  Fragments arranged by size: 

                2219    1461    1383     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3471     493 
   Size:        711    2267    2702

  Fragments arranged by size: 

                2702    2267     711
 
BsaBI GATnn'nnATC

Cuts at:   5674    5674 
   Size:       5680
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3487    3869    4504     275 
   Size:         53      83     186     936    1954     382     635    1451

  Fragments arranged by size: 

                1954    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4113    4511    4785    4932    5343    4113 
   Size:        398     274     147     411    4450

  Fragments arranged by size: 

                4450     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5455

  Fragments arranged by size: 

                5455     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2230    2239    2242    2251    2961    1186 
   Size:       1044       9       3       9     710    3905

  Fragments arranged by size: 

                3905    1044     710       9       9       3
 
BseYI C'CCAG_C

Cuts at:   2156    2435    2451    2492    2597    2603    2656    4883    5379 
   Size:        279      16      41     105       6      53    2227     496

Cuts at:   5379    2156 
   Size:       2457

  Fragments arranged by size: 

                2457    2227     496     279     105      53      41      16
                   6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5107
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3130    3891    4919    1540 
   Size:        275     186    1129     761    1028    2301

  Fragments arranged by size: 

                2301    1129    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3741    3826    4897    3741 
   Size:         85    1071    4524

  Fragments arranged by size: 

                4524    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3191    3284    3191 
   Size:         93    5587
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2553    2621    2732    2782    2999    3741    3826 
   Size:        399     763      68     111      50     217     742      85

Cuts at:   3826    4897    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     763     742     399     217     111      85
                  68      50
 
BspEI T'CCGG_A

Cuts at:   5343    5343 
   Size:       5680
 
BspHI T'CATG_A

Cuts at:   3569    5299    3569 
   Size:       1730    3950
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3035     844 
   Size:       2191    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2355    2775    3567    1394 
   Size:        429     532     420     792    3507

  Fragments arranged by size: 

                3507     792     532     429     420
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4175    4349      65 
   Size:       1183    2927     174    1396

  Fragments arranged by size: 

                2927    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2591    4324    4511    1700 
   Size:        110     781    1733     187    2869

  Fragments arranged by size: 

                2869    1733     781     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5680
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5680
 
BssSI C'ACGA_G

Cuts at:   3740    4752    3740 
   Size:       1012    4668
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2409     794 
   Size:       1615    4065
 
BstBI TT'CG_AA

Cuts at:   4484    4484 
   Size:       5680
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5680
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2113    2840    2113 
   Size:        727    4953
 
BstYI r'GATC_y

Cuts at:   1972    3010    3377    3762    3779    5231    5317    5329    5675 
   Size:       1038     367     385      17    1452      86      12     346

Cuts at:   5675    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1038     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2106     513 
   Size:        552    1041    4087

  Fragments arranged by size: 

                4087    1041     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4941

  Fragments arranged by size: 

                4941     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2145    3196    3990    4010    2145 
   Size:       1051     794      20    3815

  Fragments arranged by size: 

                3815    1051     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5680
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5645
 
DraI TTT'AAA

Cuts at:   2984    2984 
   Size:       5680
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4687     809 
   Size:        507    3371    1802

  Fragments arranged by size: 

                3371    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2115    2479    3127 
   Size:         54    1425      50     275      18     285     364     648

Cuts at:   3127    4016    5645       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     648     364     285     275      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3127    1537 
   Size:        275    1315    4090

  Fragments arranged by size: 

                4090    1315     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3610    1353 
   Size:       2257    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4267    4641    4787     387 
   Size:       1064     138    2678     374     146    1280

  Fragments arranged by size: 

                2678    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2146    2365    2547    2563    2645    3743    4340 
   Size:        393     514     219     182      16      82    1098     597

Cuts at:   4340    5127    5395    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524    1098     787     597     514     393     268     219
                 182      82      16
 
EcoO109I rG'GnC_Cy

Cuts at:   2051    2371    2550    2778    2779    2902    2946    2051 
   Size:        320     179     228       1     123      44    4785

  Fragments arranged by size: 

                4785     320     228     179     123      44       1
 
EcoRI G'AATT_C

Cuts at:   2989    3367    2989 
   Size:        378    5302
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2890    2890 
   Size:       5680
 
FspI TGC'GCA

Cuts at:   4186    4186 
   Size:       5680
 
HaeII r_GCGC'y

Cuts at:   1678    4827    1678 
   Size:       3149    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2360    2392    2938    2970    4321 
   Size:         32     135      32     788      32     546      32    1351

Cuts at:   4321    4353    4395    4427    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1351     788     546     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3024    3270     669 
   Size:        988     281    1086     246    3079

  Fragments arranged by size: 

                3079    1086     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5680
 
HpaI GTT'AAC

Cuts at:   3270    3270 
   Size:       5680
 
KpnI G_GTAC'C

Cuts at:   2885    3005    2885 
   Size:        120    5560
 
MfeI C'AATT_G

Cuts at:   3279    3279 
   Size:       5680
 
MluI A'CGCG_T

Cuts at:   5556    5556 
   Size:       5680
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2892    4794    4978    1305 
   Size:       1587    1902     184    2007

  Fragments arranged by size: 

                2007    1902    1587     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2117      10 
   Size:         54    1425     343     285    3573

  Fragments arranged by size: 

                3573    1425     343     285      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2171    2841    3638    3997    4156     518 
   Size:        648    1005     670     797     359     159    2042

  Fragments arranged by size: 

                2042    1005     797     670     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2138    2215    2533    2656    2802    3096    3773    4921 
   Size:        532      77     318     123     146     294     677    1148

Cuts at:   4921    5166    1606 
   Size:        245    2120

  Fragments arranged by size: 

                2120    1148     677     532     318     294     245     146
                 123      77
 
NcoI C'CATG_G

Cuts at:    513    1065    2106     513 
   Size:        552    1041    4087

  Fragments arranged by size: 

                4087    1041     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5680
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5680
 
NotI GC'GGCC_GC

Cuts at:   3127    3127 
   Size:       5680
 
NspI r_CATG'y

Cuts at:   1132    1229    2856    3038    4583    1132 
   Size:         97    1627     182    1545    2229

  Fragments arranged by size: 

                2229    1627    1545     182      97
 
PasI CC'CwG_GG

Cuts at:   1348    2463    2962    1348 
   Size:       1115     499    4066

  Fragments arranged by size: 

                4066    1115     499
 
PciI A'CATG_T

Cuts at:   2852    4579    2852 
   Size:       1727    3953
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2656    1108 
   Size:       1548    4132
 
PfoI T'CCnGG_A

Cuts at:   2047    2047 
   Size:       5680
 
PmeI GTTT'AAAC

Cuts at:   2984    2984 
   Size:       5680
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3749    3781    5262    5294    3749 
   Size:         32    1481      32    4135

  Fragments arranged by size: 

                4135    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2051    2946    2051 
   Size:        895    4785
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5680
 
PsiI TTA'TAA

Cuts at:   3250    3250 
   Size:       5680
 
PspOMI G'GGCC_C

Cuts at:   2549    2778    2549 
   Size:        229    5451
 
PstI C_TGCA'G

Cuts at:    830    3032     830 
   Size:       2202    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5680
 
PvuII CAG'CTG

Cuts at:   2215    2533    2656    2215 
   Size:        318     123    5239

  Fragments arranged by size: 

                5239     318     123
 
SalI G'TCGA_C

Cuts at:   1936    3022    1936 
   Size:       1086    4594
 
SanDI GG'GwC_CC

Cuts at:   2051    2051 
   Size:       5680
 
SbfI CC_TGCA'GG

Cuts at:   3032    3032 
   Size:       5680
 
ScaI AGT'ACT

Cuts at:   3928    5640    3928 
   Size:       1712    3968
 
SfcI C'TryA_G

Cuts at:    826    1045    3028    4163    4844    5035     826 
   Size:        219    1983    1135     681     191    1471

  Fragments arranged by size: 

                1983    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5680
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5680
 
SmaI CCC'GGG

Cuts at:   1872    3007    1872 
   Size:       1135    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2723    3784    4685    4947     820 
   Size:        197     926     780    1061     901     262    1553

  Fragments arranged by size: 

                1553    1061     926     901     780     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3471     493 
   Size:       2978    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5680
 
SphI G_CATG'C

Cuts at:   1132    3038    1132 
   Size:       1906    3774
 
SspI AAT'ATT

Cuts at:      5      52    3604       5 
   Size:         47    3552    2081

  Fragments arranged by size: 

                3552    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2511    1517 
   Size:        994    4686
 
StyI C'CwwG_G

Cuts at:    513    1065    2106    2763    3119     513 
   Size:        552    1041     657     356    3074

  Fragments arranged by size: 

                3074    1041     657     552     356
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2663    2722    3707    3724    3877    4062    4507 
   Size:       1255    1083      59     985      17     153     185     445

Cuts at:   4507     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255    1083     985     445     185     153      59
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3926    5638      96 
   Size:        274      80      33      51    1443    1949    1712     138

  Fragments arranged by size: 

                1949    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5640    5672    5640 
   Size:         32    5648
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5680
 
XbaI T'CTAG_A

Cuts at:   3016    3016 
   Size:       5680
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2024    2110    2605    2653    2024 
   Size:         86     495      48    5051

  Fragments arranged by size: 

                5051     495      86      48
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5680
 
XmaI C'CCGG_G

Cuts at:   1870    3005    1870 
   Size:       1135    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2630    3809    2630 
   Size:       1179    4501
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3488     276 
   Size:         53      83     186     936    1954    2468

  Fragments arranged by size: 

                2468    1954     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII     SalI    SanDI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BbeI    BfrBI     BplI
    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI    EcoRV
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI
     PacI     PmlI    PspXI     PsrI    RsrII     SacI    SacII     SapI
    SexAI     SfiI     SfoI     SrfI     SwaI
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