Restriction map for
pFN21AB9629
(Circular) (Six-base) MAPSORT of: pFN21AB9629.seq Check: 8540 from: 1 to: 5716
pFN21AB9629 5716 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3059 1937
Size: 1122 4594
Acc65I G'GTAC_C
Cuts at: 3037 3037
Size: 5716
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2843 3779 5163 5431 1632
Size: 1211 936 1384 268 1917
Fragments arranged by size:
1917 1384 1211 936 268
AflII C'TTAA_G
Cuts at: 820 1017 2353 2782 820
Size: 197 1336 429 3754
Fragments arranged by size:
3754 1336 429 197
AflIII A'CryG_T
Cuts at: 1203 4615 5592 1203
Size: 3412 977 1327
Fragments arranged by size:
3412 1327 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2895 1184
Size: 1711 4005
AlwNI CAG_nnn'CTG
Cuts at: 2392 3459 5031 2392
Size: 1067 1572 3077
Fragments arranged by size:
3077 1572 1067
ApaI G_GGCC'C
Cuts at: 2158 2158
Size: 5716
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 2448 3025 3251 3403 5512 1493
Size: 955 577 226 152 2109 1697
Fragments arranged by size:
2109 1697 955 577 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4270 160
Size: 872 3238 1606
Fragments arranged by size:
3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 2662 3041 1870
Size: 73 719 379 4545
Fragments arranged by size:
4545 719 379 73
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2912 3037 4492 618
Size: 325 217 627 1125 125 1455 1842
Fragments arranged by size:
1842 1455 1125 627 325 217 125
BanII G_rGCy'C
Cuts at: 1391 2158 2652 3035 1391
Size: 767 494 383 4072
Fragments arranged by size:
4072 767 494 383
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2199 928
Size: 971 300 4445
Fragments arranged by size:
4445 971 300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3907 3941 1903
Size: 34 1970 34 3678
Fragments arranged by size:
3678 1970 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4818 3608
Size: 1210 4506
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5716
BfrBI ATG'CAT
Cuts at: 2872 2872
Size: 5716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4327 136
Size: 107 122 71 631 605 2655 1525
Fragments arranged by size:
2655 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2473 5711 2473
Size: 3238 2478
BlpI GC'TnA_GC
Cuts at: 3133 3133
Size: 5716
Bme1580I G_kGCm'C
Cuts at: 1790 2158 2440 3777 4933 1790
Size: 368 282 1337 1156 2573
Fragments arranged by size:
2573 1337 1156 368 282
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3447 4405 442
Size: 416 53 83 399 24 2030 958 1753
Fragments arranged by size:
2030 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2181 4376 1239
Size: 942 2195 2579
Fragments arranged by size:
2579 2195 942
Bpu10I CC'TnA_GC
Cuts at: 2646 2646
Size: 5716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3841 4706 5004 3841
Size: 865 298 4553
Fragments arranged by size:
4553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2461 4379 882
Size: 617 962 1918 2219
Fragments arranged by size:
2219 1918 962 617
BsaAI yAC'GTr
Cuts at: 493 1204 3507 493
Size: 711 2303 2702
Fragments arranged by size:
2702 2303 711
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3523 3905 4540 275
Size: 53 83 186 936 1990 382 635 1451
Fragments arranged by size:
1990 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4149 4547 4821 4968 5379 4149
Size: 398 274 147 411 4486
Fragments arranged by size:
4486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5491
Fragments arranged by size:
5491 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 1186
Size: 1015 4701
BseYI C'CCAG_C
Cuts at: 2514 4919 5415 2514
Size: 2405 496 2815
Fragments arranged by size:
2815 2405 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2077 2577 2901 1163
Size: 573 341 500 324 3978
Fragments arranged by size:
3978 573 500 341 324
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3166 3927 4955 1540
Size: 275 186 1165 761 1028 2301
Fragments arranged by size:
2301 1165 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2652 3777 3862 4933 2652
Size: 1125 85 1071 3435
Fragments arranged by size:
3435 1125 1071 85
BsmI GAATG_Cn'
Cuts at: 2081 3227 3320 2081
Size: 1146 93 4477
Fragments arranged by size:
4477 1146 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2158 2440 2652 3035 3777 3862 4933
Size: 399 368 282 212 383 742 85 1071
Cuts at: 4933 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 383 368 282 212
85
BspEI T'CCGG_A
Cuts at: 5379 5379
Size: 5716
BspHI T'CATG_A
Cuts at: 3605 5335 3605
Size: 1730 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3071 844
Size: 2227 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3603 1394
Size: 429 1780 3507
Fragments arranged by size:
3507 1780 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4211 4385 65
Size: 1183 2963 174 1396
Fragments arranged by size:
2963 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4360 4547 1700
Size: 110 2550 187 2869
Fragments arranged by size:
2869 2550 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5716
BssHII G'CGCG_C
Cuts at: 1925 2306 1925
Size: 381 5335
BssSI C'ACGA_G
Cuts at: 3776 4788 3776
Size: 1012 4704
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5716
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 2607 1184
Size: 1423 4293
BstYI r'GATC_y
Cuts at: 1972 2473 3046 3413 3798 3815 5267 5353 5365
Size: 501 573 367 385 17 1452 86 12
Cuts at: 5365 5711 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 573 501 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 2722 2889 3232 4026 4046 2722
Size: 167 343 794 20 4392
Fragments arranged by size:
4392 794 343 167 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2514 2549 529
Size: 35 1950 35 3696
Fragments arranged by size:
3696 1950 35 35
DraI TTT'AAA
Cuts at: 3020 3020
Size: 5716
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4723 809
Size: 507 3407 1802
Fragments arranged by size:
3407 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2418 3163 4052
Size: 54 1425 50 275 18 588 745 889
Cuts at: 4052 5681 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 745 588 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3163 1537
Size: 275 1351 4090
Fragments arranged by size:
4090 1351 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 2196 3646 1353
Size: 825 18 1450 3423
Fragments arranged by size:
3423 1450 825 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4303 4677 4823 387
Size: 1064 138 2714 374 146 1280
Fragments arranged by size:
2714 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2650 2650
Size: 5716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 2843 3779 4376 5163 5431 1239
Size: 393 549 662 936 597 787 268 1524
Fragments arranged by size:
1524 936 787 662 597 549 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2906 2906
Size: 5716
EcoO109I rG'GnC_Cy
Cuts at: 2154 2155 2777 2897 2154
Size: 1 622 120 4973
Fragments arranged by size:
4973 622 120 1
EcoRI G'AATT_C
Cuts at: 3025 3403 3025
Size: 378 5338
FspI TGC'GCA
Cuts at: 2966 4222 2966
Size: 1256 4460
HaeII r_GCGC'y
Cuts at: 1678 4863 1678
Size: 3185 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2458 2490 4357 4389 4431
Size: 32 135 32 886 32 1867 32 42
Cuts at: 4431 4463 1373
Size: 32 2626
Fragments arranged by size:
2626 1867 886 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2010 3060 3306 669
Size: 988 281 72 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246 72
HindIII A'AGCT_T
Cuts at: 748 2110 748
Size: 1362 4354
HpaI GTT'AAC
Cuts at: 2010 3306 2010
Size: 1296 4420
KpnI G_GTAC'C
Cuts at: 3041 3041
Size: 5716
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4830 5014 1305
Size: 3525 184 2007
Fragments arranged by size:
3525 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2420 10
Size: 54 1425 343 588 3306
Fragments arranged by size:
3306 1425 588 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2877 3674 4033 4192 518
Size: 648 1711 797 359 159 2042
Fragments arranged by size:
2042 1711 797 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3132 3809 4957 5202 1606
Size: 1526 677 1148 245 2120
Fragments arranged by size:
2120 1526 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5164
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5716
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NsiI A_TGCA'T
Cuts at: 2874 2874
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 2872 3074 4619 1132
Size: 97 1643 202 1545 2229
Fragments arranged by size:
2229 1643 1545 202 97
PasI CC'CwG_GG
Cuts at: 1348 2892 1348
Size: 1544 4172
PciI A'CATG_T
Cuts at: 4615 4615
Size: 5716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5716
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2777 2897 2777
Size: 120 5596
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PspOMI G'GGCC_C
Cuts at: 2154 2154
Size: 5716
PstI C_TGCA'G
Cuts at: 830 3068 830
Size: 2238 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
SacI G_AGCT'C
Cuts at: 2652 2652
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SanDI GG'GwC_CC
Cuts at: 2897 2897
Size: 5716
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 2743 3964 5676 2743
Size: 1221 1712 2783
Fragments arranged by size:
2783 1712 1221
SfcI C'TryA_G
Cuts at: 826 1045 3064 4199 4880 5071 826
Size: 219 2019 1135 681 191 1471
Fragments arranged by size:
2019 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 2664 3043 1872
Size: 792 379 4545
Fragments arranged by size:
4545 792 379
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2353 2782 3820 4721 4983 820
Size: 197 926 410 429 1038 901 262 1553
Fragments arranged by size:
1553 1038 926 901 429 410 262 197
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 2872 3074 1132
Size: 1740 202 3774
Fragments arranged by size:
3774 1740 202
SspI AAT'ATT
Cuts at: 5 52 3640 5
Size: 47 3588 2081
Fragments arranged by size:
3588 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5716
StyI C'CwwG_G
Cuts at: 513 1065 2838 2902 3155 513
Size: 552 1773 64 253 3074
Fragments arranged by size:
3074 1773 552 253 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2905 3743 3760 3913 4098 4543 325
Size: 1255 1325 838 17 153 185 445 1498
Fragments arranged by size:
1498 1325 1255 838 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2741 3962 5674
Size: 274 80 33 51 1443 764 1221 1712
Cuts at: 5674 96
Size: 138
Fragments arranged by size:
1712 1443 1221 764 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5676 5708 5676
Size: 32 5684
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 2069 3052 2069
Size: 983 4733
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5716
XmaI C'CCGG_G
Cuts at: 1870 2662 3041 1870
Size: 792 379 4545
Fragments arranged by size:
4545 792 379
XmnI GAAnn'nnTTC
Cuts at: 2065 3845 2065
Size: 1780 3936
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI