Restriction map for
pFN21AB9623
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccIII AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AvaII AviII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmFI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DpnII DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MaeIII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI RcaI RsrII SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tsp509I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AclI 165 AACGTT AA^CGTT
AfaI 130 GTAC GT^AC
Asp700I 237 GAACCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 180 GAGCTC G[AT]GC[AT]^C
AspI 210 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 193 CCCGAG C^[CT]CG[GA]G
AvrII 342 CCTAGG C^CTAGG
BanII 180 GAGCTC G[GA]GC[CT]^C
BbsI 403 GAAGAC GAAGAC(2/6)
BfaI 343 CTAG C^TAG
BlnI 342 CCTAGG C^CTAGG
BpuAI 403 GAAGAC GAAGAC(2/6)
BsiHKAI 180 GAGCTC G[AT]GC[AT]^C
BsmI 268 GCATTC GAATGC(1/-1)
BsoBI 193 CCCGAG C^[CT]CG[GA]G
BssSI 226 CTCGTG CACGAG(-5/-1)
BstNI 126 CCAGG CC^[AT]GG
BstXI 257 CCATGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 55 CTGAG C^T[ACGT]AG
EarI 421 GAAGAG CTCTTC(1/4)
EcoRII 126 CCAGG ^CC[AT]GG
EcoT14I 342 CCTAGG C^C[AT][AT]GG
HinfI 242 GATTC G^A[ACGT]TC
Ksp632I 421 GAAGAG CTCTTC(1/4)
MaeI 343 CTAG C^TAG
MseI 170 TTAA T^TAA
MspA1I 85 CAGCTG C[AC]G^C[GT]G
MvaI 126 CCAGG CC^[AT]GG
NspI 221 ACATGC [GA]CATG^[CT]
PflFI 210 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 72 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 165 AACGTT AA^CGTT
PvuII 85 CAGCTG CAG^CTG
RsaI 130 GTAC GT^AC
SacI 180 GAGCTC GAGCT^C
StyI 342 CCTAGG C^C[AT][AT]GG
TfiI 242 GATTC G^A[AT]TC
Tru9I 170 TTAA T^TAA
TspRI 379 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 210 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 72 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 237 GAACCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccII 30 CGCG CG^CG
49 CGCG
AcyI 213 GGCGTC G[GA]^CG[CT]C
253 GACGCC
BsaHI 213 GGCGTC G[GA]^CG[CT]C
253 GACGCC
BsgI 25 CTGCAC GTGCAG(16/14)
205 GTGCAG
BstUI 30 CGCG CG^CG
49 CGCG
Cfr13I 110 GGGCC G^G[ACGT]CC
356 GGGCC
HaeIII 111 GGCC GG^CC
357 GGCC
HgaI 253 GACGC GACGC(5/10)
214 GCGTC
Hin1I 213 GGCGTC G[GA]^CG[CT]C
253 GACGCC
HphI 346 GGTGA GGTGA(8/7)
287 TCACC
MaeII 132 ACGT A^CGT
166 ACGT
MvnI 30 CGCG CG^CG
49 CGCG
Sau96I 110 GGGCC G^G[ACGT]CC
356 GGGCC
SfaNI 39 GATGC GCATC(5/9)
59 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BanI BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
Bsp1286I BssKI BstF5I Cac8I CfoI
Fnu4HI FokI HapII HhaI HinP1I
HpaII ItaI MboII MnlI MslI
MspI MwoI NciI NlaIII NlaIV
ScrFI TaqI TseI TthHB8I