Restriction map for
pFN21AB9606
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI AspI AviII AvrII
BalI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsmI BspDI
BspEI BspLU11I BspMI BsrDI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KasI KspI MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI Tru9I Tsp45I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 818 GGTACC G^GTACC
AhdI 283 GACGACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 525 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 818 GGTACC G^GTACC
AspEI 283 GACGACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 856 GAGCTC G[AT]GC[AT]^C
AvaII 987 GGACC G^G[AT]CC
BamHI 563 GGATCC G^GATCC
BclI 633 TGATCA T^GATCA
BcnI 175 CCGGG CC^[GC]GG
BglI 95 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 1033 CTGCAC GTGCAG(16/14)
BsiEI 596 CGGTCG CG[GA][CT]^CG
BsiHKAI 856 GAGCTC G[AT]GC[AT]^C
BsiWI 809 CGTACG C^GTACG
Bsp1407I 52 TGTACA T^GTACA
BspHI 366 TCATGA T^CATGA
BsrGI 52 TGTACA T^GTACA
BssHII 996 GCGCGC G^CGCGC
Eam1105I 283 GACGACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 584 CCAAGG C^C[AT][AT]GG
FbaI 633 TGATCA T^GATCA
HphI 736 GGTGA GGTGA(8/7)
KpnI 818 GGTACC GGTAC^C
MaeII 808 ACGT A^CGT
NaeI 315 GCCGGC GCC^GGC
NciI 175 CCGGG CC^[GC]GG
NgoMIV 315 GCCGGC G^CCGGC
PshAI 464 GACGCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 366 TCATGA T^CATGA
SacI 856 GAGCTC GAGCT^C
SapI 76 GAAGAGC GCTCTTC(1/4)
SspBI 52 TGTACA T^GTACA
StuI 230 AGGCCT AGG^CCT
StyI 584 CCAAGG C^C[AT][AT]GG
XmnI 525 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 333 GACGCC G[GA]^CG[CT]C
464 GACGCC
AlwNI 56 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1012 CAGCTGCTG
AvaI 121 CCCGAG C^[CT]CG[GA]G
337 CCCGAG
BanI 117 GGTGCC G^G[CT][GA]CC
818 GGTACC
BanII 392 GGGCTC G[GA]GC[CT]^C
856 GAGCTC
BfaI 44 CTAG C^TAG
245 CTAG
BsaHI 333 GACGCC G[GA]^CG[CT]C
464 GACGCC
BsmBI 462 GAGACG CGTCTC(1/5)
805 GAGACG
BsoBI 121 CCCGAG C^[CT]CG[GA]G
337 CCCGAG
BsrBI 112 GAGCGG CCGCTC(-3/-3)
814 GAGCGG
BstXI 159 CCAGTTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
380 CCAGTAAGTTGG
BstYI 563 GGATCC [GA]^GATC[CT]
654 GGATCT
DdeI 89 CTGAG C^T[ACGT]AG
253 CTGAG
EaeI 2 TGGCCG [CT]^GGCC[GA]
168 TGGCCG
EcoNI 252 CCTGAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
397 CCTGCCGCAGG
HaeII 92 AGCGCC [GA]GCGC^[CT]
1053 AGCGCC
Hin1I 333 GACGCC G[GA]^CG[CT]C
464 GACGCC
MaeI 44 CTAG C^TAG
245 CTAG
MflI 563 GGATCC [GA]^GATC[CT]
654 GGATCT
PleI 286 GACTC GAGTC(4/5)
370 GACTC
PstI 787 CTGCAG CTGCA^G
835 CTGCAG
PvuII 439 CAGCTG CAG^CTG
1012 CAGCTG
SfcI 787 CTGCAG C^T[GA][CT]AG
835 CTGCAG
TfiI 202 GAATC G^A[AT]TC
241 GAATC
XhoII 563 GGATCC [GA]^GATC[CT]
654 GGATCT
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DpnII EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
TaqI TseI TthHB8I