(Circular) (Six-base) MAPSORT of: pFN21AB9606.seq Check: 7503 from: 1 to: 5782 pFN21AB9606 5782 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 1937 Size: 1188 4594 Acc65I G'GTAC_C Cuts at: 2823 3103 2823 Size: 280 5502 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3845 5229 5497 1632 Size: 2213 1384 268 1917 Fragments arranged by size: 2213 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 4681 5658 1203 Size: 3478 977 1327 Fragments arranged by size: 3478 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 2293 4511 2293 Size: 2218 3564 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AlwNI CAG_nnn'CTG Cuts at: 2066 3022 3525 5097 2066 Size: 956 503 1572 2751 Fragments arranged by size: 2751 1572 956 503 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 3091 3317 3469 5578 1493 Size: 1598 226 152 2109 1697 Fragments arranged by size: 2109 1697 1598 226 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2126 2342 3107 1870 Size: 73 183 216 765 4545 Fragments arranged by size: 4545 765 216 183 73 BamHI G'GATC_C Cuts at: 2568 3112 3479 2568 Size: 544 367 4871 Fragments arranged by size: 4871 544 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 2823 3103 4558 618 Size: 325 217 627 335 701 280 1455 1842 Fragments arranged by size: 1842 1455 701 627 335 325 280 217 BanII G_rGCy'C Cuts at: 1391 2401 2865 3101 1391 Size: 1010 464 236 4072 Fragments arranged by size: 4072 1010 464 236 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4811 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3973 4007 1903 Size: 34 2036 34 3678 Fragments arranged by size: 3678 2036 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 2638 1548 Size: 1090 4692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2106 4393 136 Size: 107 122 71 631 605 434 2287 1525 Fragments arranged by size: 2287 1525 631 605 434 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 3199 3199 Size: 5782 Bme1580I G_kGCm'C Cuts at: 1790 2127 3843 4999 1790 Size: 337 1716 1156 2573 Fragments arranged by size: 2573 1716 1156 337 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2158 3513 4471 Size: 416 53 83 399 24 741 1355 958 Cuts at: 4471 442 Size: 1753 Fragments arranged by size: 1753 1355 958 741 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2906 2954 2999 3005 4442 1239 Size: 1667 48 45 6 1437 2579 Fragments arranged by size: 2579 1667 1437 48 45 6 Bpu10I CC'TnA_GC Cuts at: 2094 2094 Size: 5782 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3907 4772 5070 3907 Size: 865 298 4619 Fragments arranged by size: 4619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4445 882 Size: 617 2946 2219 Fragments arranged by size: 2946 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3573 493 Size: 711 2369 2702 Fragments arranged by size: 2702 2369 711 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2339 2470 3589 3971 Size: 53 83 186 936 806 131 1119 382 Cuts at: 3971 4606 275 Size: 635 1451 Fragments arranged by size: 1451 1119 936 806 635 382 186 131 83 53 BsaWI w'CCGG_w Cuts at: 4215 4613 4887 5034 5445 4215 Size: 398 274 147 411 4552 Fragments arranged by size: 4552 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5557 Fragments arranged by size: 5557 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2144 2435 2666 2759 1186 Size: 943 15 291 231 93 4209 Fragments arranged by size: 4209 943 291 231 93 15 BseYI C'CCAG_C Cuts at: 2361 2446 2947 4985 5481 2361 Size: 85 501 2038 496 2662 Fragments arranged by size: 2662 2038 501 496 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3023 1163 Size: 573 1287 3922 Fragments arranged by size: 3922 1287 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2604 3232 3993 5021 1540 Size: 275 186 603 628 761 1028 2301 Fragments arranged by size: 2301 1028 761 628 603 275 186 BsiHKAI G_wGCw'C Cuts at: 2865 3843 3928 4999 2865 Size: 978 85 1071 3648 Fragments arranged by size: 3648 1071 978 85 BsiWI C'GTAC_G Cuts at: 2814 2814 Size: 5782 BsmI GAATG_Cn' Cuts at: 3293 3386 3293 Size: 93 5689 BsmBI CGTCTCn'nnnn_ Cuts at: 2461 2804 3072 2461 Size: 343 268 5171 Fragments arranged by size: 5171 343 268 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2401 2865 3101 3843 3928 4999 Size: 399 337 274 464 236 742 85 1071 Cuts at: 4999 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 464 399 337 274 236 85 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 2371 3671 5401 2371 Size: 1300 1730 2752 Fragments arranged by size: 2752 1730 1300 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3137 844 Size: 2293 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2119 2821 3669 1394 Size: 429 296 702 848 3507 Fragments arranged by size: 3507 848 702 429 296 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2320 2599 2669 2922 2994 3042 4426 Size: 110 510 279 70 253 72 48 1384 Cuts at: 4426 4613 1700 Size: 187 2869 Fragments arranged by size: 2869 1384 510 279 253 187 110 72 70 48 BsrGI T'GTAC_A Cuts at: 96 2057 96 Size: 1961 3821 BssHII G'CGCG_C Cuts at: 1925 3001 1925 Size: 1076 4706 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5782 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstXI CCAn_nnnn'nTGG Cuts at: 2171 2392 2171 Size: 221 5561 BstYI r'GATC_y Cuts at: 1972 2568 2659 3112 3479 3864 3881 5333 5419 Size: 596 91 453 367 385 17 1452 86 Cuts at: 5419 5431 5777 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 596 453 385 367 346 91 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4291 Fragments arranged by size: 4291 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2730 2775 487 Size: 683 56 1504 45 3494 Fragments arranged by size: 3494 1504 683 56 45 BtsI GCAGTG_nn' Cuts at: 3298 4092 4112 3298 Size: 794 20 4968 Fragments arranged by size: 4968 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2205 2240 529 Size: 35 1641 35 4071 Fragments arranged by size: 4071 1641 35 35 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2173 3229 Size: 54 1425 50 275 18 177 166 1056 Cuts at: 3229 4118 5747 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1056 889 275 177 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 2412 2517 3712 1353 Size: 723 336 105 1195 3423 Fragments arranged by size: 3423 1195 723 336 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 4743 4889 387 Size: 1064 138 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2863 2863 Size: 5782 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2906 2954 2999 3005 3845 4442 5229 Size: 393 1274 48 45 6 840 597 787 Cuts at: 5229 5497 1239 Size: 268 1524 Fragments arranged by size: 1524 1274 840 787 597 393 268 48 45 6 EcoNI CCTnn'n_nnAGG Cuts at: 2261 2406 2261 Size: 145 5637 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 2101 3062 4929 1678 Size: 423 961 1867 2531 Fragments arranged by size: 2531 1867 961 423 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2279 2311 4423 4455 4497 Size: 32 135 32 707 32 2112 32 42 Cuts at: 4497 4529 1373 Size: 32 2626 Fragments arranged by size: 2626 2112 707 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3126 3372 669 Size: 988 281 1188 246 3079 Fragments arranged by size: 3079 1188 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 2827 3107 2827 Size: 280 5502 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2727 4896 5080 1305 Size: 1422 2169 184 2007 Fragments arranged by size: 2169 2007 1422 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3740 4099 4258 518 Size: 648 2574 359 159 2042 Fragments arranged by size: 2574 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2300 2446 2797 3019 3198 3875 5023 5268 Size: 694 146 351 222 179 677 1148 245 Cuts at: 5268 1606 Size: 2120 Fragments arranged by size: 2120 1148 694 677 351 245 222 179 146 NaeI GCC'GGC Cuts at: 2322 2322 Size: 5782 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4291 Fragments arranged by size: 4291 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NgoMIV G'CCGG_C Cuts at: 2320 2320 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 3140 4685 1132 Size: 97 1911 1545 2229 Fragments arranged by size: 2229 1911 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5782 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5782 PfoI T'CCnGG_A Cuts at: 2076 2076 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2473 1613 Size: 860 4922 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 2796 2844 3134 830 Size: 1966 48 290 3478 Fragments arranged by size: 3478 1966 290 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2446 3019 2446 Size: 573 5209 SacI G_AGCT'C Cuts at: 2865 2865 Size: 5782 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SapI GCTCTTCn'nnn_ Cuts at: 2076 2076 Size: 5782 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 2792 2840 3130 4265 4946 5137 826 Size: 219 1747 48 290 1135 681 191 1471 Fragments arranged by size: 1747 1471 1135 681 290 219 191 48 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3886 4787 5049 820 Size: 197 926 1943 901 262 1553 Fragments arranged by size: 1943 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 2237 1517 Size: 720 5062 StyI C'CwwG_G Cuts at: 513 1065 2004 2589 3221 513 Size: 552 939 585 632 3074 Fragments arranged by size: 3074 939 632 585 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2159 2939 3041 3809 3826 3979 4164 Size: 1255 579 780 102 768 17 153 185 Cuts at: 4164 4609 325 Size: 445 1498 Fragments arranged by size: 1498 1255 780 768 579 445 185 153 102 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2057 2676 4028 Size: 274 80 33 51 1443 80 619 1352 Cuts at: 4028 5740 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1352 619 274 138 80 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2126 2158 5742 5774 2126 Size: 32 3584 32 2134 Fragments arranged by size: 3584 2134 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5782 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 2534 3911 2534 Size: 1377 4405 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI