Restriction map for
pFN21AB9580
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwI AlwNI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AvaI AviII AvrII
BalI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 26 GTCTAC GT^[AC][GT]AC
Alw44I 115 GTGCAC G^TGCAC
ApaLI 115 GTGCAC G^TGCAC
Asp700I 482 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 115 GTGCAC G[AT]GC[AT]^C
AspI 75 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 80 GGTCC G^G[AT]CC
BanII 94 GAGCCC G[GA]GC[CT]^C
BbrPI 112 CACGTG CAC^GTG
BfaI 23 CTAG C^TAG
BpmI 91 CTGGAG CTGGAG(16/14)
BsaAI 112 CACGTG [CT]AC^GT[GA]
BsaI 463 GAGACC GGTCTC(1/5)
BsiHKAI 115 GTGCAC G[AT]GC[AT]^C
BspMI 431 ACCTGC ACCTGC(4/8)
BsrBI 298 GAGCGG CCGCTC(-3/-3)
BstF5I 137 CATCC GGATG(2/0)
DpnII 414 GATC ^GATC
EarI 440 CTCTTC CTCTTC(1/4)
EcoT14I 98 CCATGG C^C[AT][AT]GG
FokI 137 CATCC GGATG(9/13)
Ksp632I 440 CTCTTC CTCTTC(1/4)
MaeI 23 CTAG C^TAG
MaeII 113 ACGT A^CGT
MaeIII 103 GTCAC ^GT[ACGT]AC
MboI 414 GATC ^GATC
MseI 425 TTAA T^TAA
MspA1I 310 CAGCGG C[AC]G^C[GT]G
NcoI 98 CCATGG C^CATGG
NdeII 414 GATC ^GATC
PflFI 75 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 112 CACGTG CAC^GTG
PmlI 112 CACGTG CAC^GTG
PpuMI 79 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 433 CTGCAG CTGCA^G
Sau3AI 414 GATC ^GATC
ScaI 260 AGTACT AGT^ACT
SexAI 76 ACCAGGT A^CC[AT]GGT
SfaNI 231 GATGC GCATC(5/9)
SfcI 433 CTGCAG C^T[GA][CT]AG
StyI 98 CCATGG C^C[AT][AT]GG
TfiI 55 GATTC G^A[AT]TC
Tru9I 425 TTAA T^TAA
Tsp45I 103 GTCAC ^GT[GC]AC
Tth111I 75 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 482 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BcnI 129 CCCGG CC^[GC]GG
284 CCCGG
BmyI 94 GAGCCC G[AGT]GC[ACT]^C
115 GTGCAC
BseRI 151 GAGGAG GAGGAG(10/8)
364 GAGGAG
BsiYI 278 CCTTCACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
284 CCCGGAAAAGG
BslI 278 CCTTCACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
284 CCCGGAAAAGG
BsmAI 463 GAGAC GTCTC(1/5)
438 GTCTC
Bsp1286I 94 GAGCCC G[AGT]GC[ACT]^C
115 GTGCAC
DraII 79 AGGTCCT [GA]G^G[ACGT]CC[CT]
125 AGGCCCC
DsaI 98 CCATGG C^C[GA][CT]GG
160 CCACGG
EcoO109I 79 AGGTCCT [GA]G^G[ACGT]CC[CT]
125 AGGCCCC
HaeIII 126 GGCC GG^CC
272 GGCC
HapII 130 CCGG C^CGG
285 CCGG
HpaII 130 CCGG C^CGG
285 CCGG
HphI 315 GGTGA GGTGA(8/7)
281 TCACC
MboII 47 TCTTC GAAGA(8/7)
441 TCTTC
MspI 130 CCGG C^CGG
285 CCGG
NciI 129 CCCGG CC^[GC]GG
284 CCCGG
NlaIV 93 GGAGCC GG[ACGT]^[ACGT]CC
126 GGCCCC
PleI 181 GACTC GAGTC(4/5)
383 GACTC
TspRI 71 CACTG CA[GC]TG[ACGT][ACGT]^
204 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BssKI BstNI Cac8I Cfr13I DdeI
EcoRII FauI Fnu4HI HinfI ItaI
MnlI MvaI MwoI NlaIII RsaI
Sau96I ScrFI TseI Tsp509I