Restriction map for
pFN21AB9549
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspHI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoT22I FbaI FseI HaeII Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MroI MseI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI Tru9I Van91I XcmI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Alw44I 831 GTGCAC G^TGCAC
AlwI 432 GATCC GGATC(4/5)
ApaLI 831 GTGCAC G^TGCAC
AspI 1347 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1320 TGCGCA TGC^GCA
BfaI 55 CTAG C^TAG
BsaI 844 GAGACC GGTCTC(1/5)
BseRI 234 CTCCTC GAGGAG(10/8)
BsiEI 417 CGACCG CG[GA][CT]^CG
BsmI 524 GAATGC GAATGC(1/-1)
Bsp1407I 1212 TGTACA T^GTACA
BspMI 1232 ACCTGC ACCTGC(4/8)
BsrDI 920 CATTGC GCAATG(2/0)
BsrFI 46 GCCGGC [GA]^CCGG[CT]
BsrGI 1212 TGTACA T^GTACA
BssSI 1019 CTCGTG CACGAG(-5/-1)
BstAPI 1323 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 767 CCAAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 46 GCCGGC [GA]^CCGG[CT]
EcoT14I 872 CCAAGG C^C[AT][AT]GG
FspI 1320 TGCGCA TGC^GCA
HgaI 756 GACGC GACGC(5/10)
HindIII 141 AAGCTT A^AGCTT
MaeI 55 CTAG C^TAG
MluI 350 ACGCGT A^CGCGT
NaeI 46 GCCGGC GCC^GGC
NgoMIV 46 GCCGGC G^CCGGC
PaeR7I 40 CTCGAG C^TCGAG
PflFI 1347 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 856 GAGCTC GAGCT^C
SmaI 674 CCCGGG CCC^GGG
SphI 1317 GCATGC GCATG^C
SspBI 1212 TGTACA T^GTACA
StyI 872 CCAAGG C^C[AT][AT]GG
Tth111I 1347 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 54 TCTAGA T^CTAGA
XhoI 40 CTCGAG C^TCGAG
XmaCI 674 CCCGGG C^CCGGG
XmaI 674 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 351 CGCG CG^CG
507 CGCG
AfaI 1213 GTAC GT^AC
1401 GTAC
AocI 326 CCTCAGG CC^T[ACGT]AGG
1038 CCTAAGG
Asp700I 645 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1044 GAAACCGTTC
AvaI 40 CTCGAG C^[CT]CG[GA]G
674 CCCGGG
BsgI 1366 CTGCAC GTGCAG(16/14)
1330 GTGCAG
BsoBI 40 CTCGAG C^[CT]CG[GA]G
674 CCCGGG
BspLU11I 1215 ACATGT A^CATGT
1397 ACATGT
BstUI 351 CGCG CG^CG
507 CGCG
Bsu36I 326 CCTCAGG CC^T[ACGT]AGG
1038 CCTAAGG
Cac8I 46 GCCGGC GC[ACGT]^[ACGT]GC
1317 GCATGC
CfoI 1321 GCGC GCG^C
1363 GCGC
EarI 1264 CTCTTC CTCTTC(1/4)
73 GAAGAG
Eco81I 326 CCTCAGG CC^T[ACGT]AGG
1038 CCTAAGG
EcoRI 865 GAATTC G^AATTC
1024 GAATTC
FauI 1371 CCCGC CCCGC(4/6)
314 GCGGG
HhaI 1321 GCGC GCG^C
1363 GCGC
HinP1I 1321 GCGC G^CGC
1363 GCGC
Ksp632I 1264 CTCTTC CTCTTC(1/4)
73 GAAGAG
MaeII 835 ACGT A^CGT
1178 ACGT
MslI 349 CACGCGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
834 CACGTCAGTG
MvnI 351 CGCG CG^CG
507 CGCG
NlaIV 255 GGTCCC GG[ACGT]^[ACGT]CC
1065 GGAACC
RsaI 1213 GTAC GT^AC
1401 GTAC
SmlI 40 CTCGAG C^T[CT][GA]AG
394 CTCAAG
StuI 323 AGGCCT AGG^CCT
635 AGGCCT
XmnI 645 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1044 GAAACCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AflIII AluI ApoI
AspHI AvaII BalI BanII BbsI
BbvI BcnI BglII BmyI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EaeI EcoRII EcoRV Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NspI PleI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII