(Circular) (Six-base) MAPSORT of: pFN21AB9549.seq Check: 5804 from: 1 to: 6109 pFN21AB9549 6109 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3246 3246 Size: 6109 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3919 278 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3452 1937 Size: 1515 4594 Acc65I G'GTAC_C Cuts at: 3430 3430 Size: 6109 AclI AA'CG_TT Cuts at: 1563 4236 4609 1563 Size: 2673 373 3063 Fragments arranged by size: 3063 2673 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2097 4172 5556 5824 1632 Size: 465 2075 1384 268 1917 Fragments arranged by size: 2075 1917 1384 465 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5912 AflIII A'CryG_T Cuts at: 1203 2355 3220 3402 5008 5985 1203 Size: 1152 865 182 1606 977 1327 Fragments arranged by size: 1606 1327 1152 977 865 182 AgeI A'CCGG_T Cuts at: 4940 4940 Size: 6109 AhdI GACnn_n'nnGTC Cuts at: 4838 4838 Size: 6109 AleI CACnn'nnGTG Cuts at: 1166 2358 2843 1166 Size: 1192 485 4432 Fragments arranged by size: 4432 1192 485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6109 AlwNI CAG_nnn'CTG Cuts at: 3852 5424 3852 Size: 1572 4537 ApaLI G'TGCA_C Cuts at: 2836 4166 5322 2836 Size: 1330 1156 3623 Fragments arranged by size: 3623 1330 1156 ApoI r'AATT_y Cuts at: 1493 2565 2870 3029 3418 3644 3796 5905 1493 Size: 1072 305 159 389 226 152 2109 1697 Fragments arranged by size: 2109 1697 1072 389 305 226 159 152 AseI AT'TA_AT Cuts at: 160 1032 4663 160 Size: 872 3631 1606 Fragments arranged by size: 3631 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 AvaI C'yCGr_G Cuts at: 1870 1943 2045 2679 3434 1870 Size: 73 102 634 755 4545 Fragments arranged by size: 4545 755 634 102 73 BamHI G'GATC_C Cuts at: 3439 3806 3439 Size: 367 5742 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3430 4885 618 Size: 325 217 627 1643 1455 1842 Fragments arranged by size: 1842 1643 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2069 2237 2865 3428 1391 Size: 678 168 628 563 4072 Fragments arranged by size: 4072 678 628 563 168 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2765 2961 3356 928 Size: 971 866 196 395 3681 Fragments arranged by size: 3681 971 866 395 196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4300 4334 1903 Size: 34 2363 34 3678 Fragments arranged by size: 3678 2363 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4001 5211 4001 Size: 1210 4899 BclI T'GATC_A Cuts at: 1548 1548 Size: 6109 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4720 136 Size: 107 122 71 631 605 3048 1525 Fragments arranged by size: 3048 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2537 2608 3390 6104 2537 Size: 71 782 2714 2542 Fragments arranged by size: 2714 2542 782 71 BlpI GC'TnA_GC Cuts at: 3526 3526 Size: 6109 Bme1580I G_kGCm'C Cuts at: 1790 2840 4170 5326 1790 Size: 1050 1330 1156 2573 Fragments arranged by size: 2573 1330 1156 1050 BmgBI CAC'GTC Cuts at: 1318 2841 1318 Size: 1523 4586 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2280 3840 4798 Size: 416 53 83 399 24 863 1560 958 Cuts at: 4798 442 Size: 1753 Fragments arranged by size: 1753 1560 958 863 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4769 1239 Size: 3530 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2384 4234 5099 5397 2384 Size: 1850 865 298 3096 Fragments arranged by size: 3096 1850 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2843 4772 882 Size: 617 1344 1929 2219 Fragments arranged by size: 2219 1929 1344 617 BsaAI yAC'GTr Cuts at: 493 1204 3900 493 Size: 711 2696 2702 Fragments arranged by size: 2702 2696 711 BsaBI GATnn'nnATC Cuts at: 6103 6103 Size: 6109 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3916 4298 4933 275 Size: 53 83 186 936 2383 382 635 1451 Fragments arranged by size: 2383 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4542 4940 5214 5361 5772 4542 Size: 398 274 147 411 4879 Fragments arranged by size: 4879 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2729 2759 1178 Size: 30 165 30 1326 30 4528 Fragments arranged by size: 4528 1326 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2230 1186 Size: 1044 5065 BseYI C'CCAG_C Cuts at: 5312 5808 5312 Size: 496 5613 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3356 1163 Size: 573 1620 3916 Fragments arranged by size: 3916 1620 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2425 3559 4320 5348 1540 Size: 275 186 424 1134 761 1028 2301 Fragments arranged by size: 2301 1134 1028 761 424 275 186 BsiHKAI G_wGCw'C Cuts at: 2840 2865 3228 4170 4255 5326 2840 Size: 25 363 942 85 1071 3623 Fragments arranged by size: 3623 1071 942 363 85 25 BsmI GAATG_Cn' Cuts at: 2535 3620 3713 2535 Size: 1085 93 4931 Fragments arranged by size: 4931 1085 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2237 2840 2865 3228 3428 4170 Size: 399 279 168 603 25 363 200 742 Cuts at: 4170 4255 5326 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 603 399 363 279 200 168 85 25 BspEI T'CCGG_A Cuts at: 5772 5772 Size: 6109 BspHI T'CATG_A Cuts at: 3998 5728 3998 Size: 1730 4379 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3246 3464 844 Size: 2402 218 3489 Fragments arranged by size: 3489 2402 218 BsrBI CCG'CTC Cuts at: 1394 1823 3996 1394 Size: 429 2173 3507 Fragments arranged by size: 3507 2173 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2924 4604 4778 65 Size: 1183 1676 1680 174 1396 Fragments arranged by size: 1680 1676 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2051 4753 4940 1700 Size: 110 241 2702 187 2869 Fragments arranged by size: 2869 2702 241 187 110 BsrGI T'GTAC_A Cuts at: 96 3217 96 Size: 3121 2988 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6109 BssSI C'ACGA_G Cuts at: 3024 4169 5181 3024 Size: 1145 1012 3952 Fragments arranged by size: 3952 1145 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3334 794 Size: 2540 3569 BstBI TT'CG_AA Cuts at: 4913 4913 Size: 6109 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6109 BstXI CCAn_nnnn'nTGG Cuts at: 2779 2779 Size: 6109 BstYI r'GATC_y Cuts at: 1972 2436 2537 2608 3390 3439 3806 4191 4208 Size: 464 101 71 782 49 367 385 17 Cuts at: 4208 5660 5746 5758 6104 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 782 464 385 367 346 101 86 71 49 17 12 Bsu36I CC'TnA_GG Cuts at: 2332 3044 2332 Size: 712 5397 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4618 Fragments arranged by size: 4618 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5370 Fragments arranged by size: 5370 683 56 BtsI GCAGTG_nn' Cuts at: 2824 3625 4419 4439 2824 Size: 801 794 20 4494 Fragments arranged by size: 4494 801 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6109 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6074 DraI TTT'AAA Cuts at: 3413 3413 Size: 6109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5116 809 Size: 507 3800 1802 Fragments arranged by size: 3800 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2622 2769 2781 Size: 54 1425 50 275 18 792 147 12 Cuts at: 2781 3556 4445 6074 8 Size: 775 889 1629 43 Fragments arranged by size: 1629 1425 889 792 775 275 147 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3556 1537 Size: 275 1744 4090 Fragments arranged by size: 4090 1744 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2073 3275 4039 1353 Size: 720 1202 764 3423 Fragments arranged by size: 3423 1202 764 720 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2822 4696 5070 5216 387 Size: 1064 138 1233 1874 374 146 1280 Fragments arranged by size: 1874 1280 1233 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2863 2863 Size: 6109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 4172 4769 5556 5824 1239 Size: 393 465 2075 597 787 268 1524 Fragments arranged by size: 2075 1524 787 597 465 393 268 EcoRI G'AATT_C Cuts at: 2870 3029 3418 3796 2870 Size: 159 389 378 5183 Fragments arranged by size: 5183 389 378 159 EcoRV GAT'ATC Cuts at: 3064 3127 3262 3064 Size: 63 135 5911 Fragments arranged by size: 5911 135 63 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2395 2395 Size: 6109 FspI TGC'GCA Cuts at: 3327 4615 3327 Size: 1288 4821 HaeII r_GCGC'y Cuts at: 1678 5256 1678 Size: 3578 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2600 2632 4750 Size: 32 135 32 743 32 253 32 2118 Cuts at: 4750 4782 4824 4856 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2118 743 253 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3453 3699 669 Size: 988 281 1515 246 3079 Fragments arranged by size: 3079 1515 988 281 246 HindIII A'AGCT_T Cuts at: 748 2146 748 Size: 1398 4711 HpaI GTT'AAC Cuts at: 3699 3699 Size: 6109 KpnI G_GTAC'C Cuts at: 3434 3434 Size: 6109 MfeI C'AATT_G Cuts at: 3708 3708 Size: 6109 MluI A'CGCG_T Cuts at: 2355 5985 2355 Size: 3630 2479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3339 5223 5407 1305 Size: 2034 1884 184 2007 Fragments arranged by size: 2034 2007 1884 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2624 2771 2783 10 Size: 54 1425 343 792 147 12 3336 Fragments arranged by size: 3336 1425 792 343 147 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 2358 2843 4067 4426 4585 518 Size: 648 1192 485 1224 359 159 2042 Fragments arranged by size: 2042 1224 1192 648 485 359 159 MspA1I CmG'CkG Cuts at: 1606 2430 3310 3331 3525 4202 5350 5595 1606 Size: 824 880 21 194 677 1148 245 2120 Fragments arranged by size: 2120 1148 880 824 677 245 194 21 NaeI GCC'GGC Cuts at: 2053 2053 Size: 6109 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4618 Fragments arranged by size: 4618 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6109 NgoMIV G'CCGG_C Cuts at: 2051 2051 Size: 6109 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6109 NotI GC'GGCC_GC Cuts at: 3556 3556 Size: 6109 NspI r_CATG'y Cuts at: 1132 1229 3224 3326 3406 3467 5012 1132 Size: 97 1995 102 80 61 1545 2229 Fragments arranged by size: 2229 1995 1545 102 97 80 61 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6109 PciI A'CATG_T Cuts at: 3220 3402 5008 3220 Size: 182 1606 4321 Fragments arranged by size: 4321 1606 182 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6109 PmeI GTTT'AAAC Cuts at: 3413 3413 Size: 6109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4178 4210 5691 5723 4178 Size: 32 1481 32 4564 Fragments arranged by size: 4564 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6109 PsiI TTA'TAA Cuts at: 3020 3679 3020 Size: 659 5450 PspXI vC'TCGA_Gb Cuts at: 2045 2045 Size: 6109 PstI C_TGCA'G Cuts at: 830 2608 3243 3387 3461 830 Size: 1778 635 144 74 3478 Fragments arranged by size: 3478 1778 635 144 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6109 PvuII CAG'CTG Cuts at: 2430 3310 3331 2430 Size: 880 21 5208 Fragments arranged by size: 5208 880 21 SacI G_AGCT'C Cuts at: 2865 2865 Size: 6109 SalI G'TCGA_C Cuts at: 1936 3451 1936 Size: 1515 4594 SbfI CC_TGCA'GG Cuts at: 3461 3461 Size: 6109 ScaI AGT'ACT Cuts at: 4357 6069 4357 Size: 1712 4397 SfcI C'TryA_G Cuts at: 826 1045 2604 3239 3383 3394 3457 4592 5273 Size: 219 1559 635 144 11 63 1135 681 Cuts at: 5273 5464 826 Size: 191 1471 Fragments arranged by size: 1559 1471 1135 681 635 219 191 144 63 11 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6109 SmaI CCC'GGG Cuts at: 1872 2681 3436 1872 Size: 809 755 4545 Fragments arranged by size: 4545 809 755 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 2399 4213 5114 5376 820 Size: 197 926 102 354 1814 901 262 1553 Fragments arranged by size: 1814 1553 926 901 354 262 197 102 SnaBI TAC'GTA Cuts at: 493 3900 493 Size: 3407 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6109 SphI G_CATG'C Cuts at: 1132 3326 3467 1132 Size: 2194 141 3774 Fragments arranged by size: 3774 2194 141 SspI AAT'ATT Cuts at: 5 52 4033 5 Size: 47 3981 2081 Fragments arranged by size: 3981 2081 47 StuI AGG'CCT Cuts at: 1517 2330 2642 1517 Size: 813 312 4984 Fragments arranged by size: 4984 813 312 StyI C'CwwG_G Cuts at: 513 1065 2004 2877 3548 513 Size: 552 939 873 671 3074 Fragments arranged by size: 3074 939 873 671 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2191 4136 4153 4306 4491 4936 325 Size: 1255 611 1945 17 153 185 445 1498 Fragments arranged by size: 1945 1498 1255 611 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3217 4355 6067 Size: 274 80 33 51 1443 1240 1138 1712 Cuts at: 6067 96 Size: 138 Fragments arranged by size: 1712 1443 1240 1138 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2729 2761 6069 6101 2729 Size: 32 3308 32 2737 Fragments arranged by size: 3308 2737 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3355 1316 Size: 2039 4070 XbaI T'CTAG_A Cuts at: 2059 3445 2059 Size: 1386 4723 XhoI C'TCGA_G Cuts at: 1943 2045 1943 Size: 102 6007 XmaI C'CCGG_G Cuts at: 1870 2679 3434 1870 Size: 809 755 4545 Fragments arranged by size: 4545 809 755 XmnI GAAnn'nnTTC Cuts at: 2654 3053 4238 2654 Size: 399 1185 4525 Fragments arranged by size: 4525 1185 399 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3917 276 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI