(Circular) (Six-base) MAPSORT of: pFN21AB9542.seq Check: 6662 from: 1 to: 5575 pFN21AB9542 5575 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3385 278 Size: 53 83 186 936 1849 2468 Fragments arranged by size: 2468 1849 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2918 1937 Size: 981 4594 Acc65I G'GTAC_C Cuts at: 2896 2896 Size: 5575 AclI AA'CG_TT Cuts at: 1563 3702 4075 1563 Size: 2139 373 3063 Fragments arranged by size: 3063 2139 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2069 2279 2673 2799 3638 5022 5290 1632 Size: 437 210 394 126 839 1384 268 1917 Fragments arranged by size: 1917 1384 839 437 394 268 210 126 AfeI AGC'GCT Cuts at: 2523 2523 Size: 5575 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5378 AflIII A'CryG_T Cuts at: 1203 2138 2503 4474 5451 1203 Size: 935 365 1971 977 1327 Fragments arranged by size: 1971 1327 977 935 365 AgeI A'CCGG_T Cuts at: 2796 4406 2796 Size: 1610 3965 AhdI GACnn_n'nnGTC Cuts at: 4304 4304 Size: 5575 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5575 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5575 AlwNI CAG_nnn'CTG Cuts at: 2650 3318 4890 2650 Size: 668 1572 3335 Fragments arranged by size: 3335 1572 668 ApaLI G'TGCA_C Cuts at: 3632 4788 3632 Size: 1156 4419 ApoI r'AATT_y Cuts at: 1493 2884 3110 3262 5371 1493 Size: 1391 226 152 2109 1697 Fragments arranged by size: 2109 1697 1391 226 152 AseI AT'TA_AT Cuts at: 160 1032 2157 2210 4129 160 Size: 872 1125 53 1919 1606 Fragments arranged by size: 1919 1606 1125 872 53 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 AvaI C'yCGr_G Cuts at: 1870 1943 2900 1870 Size: 73 957 4545 Fragments arranged by size: 4545 957 73 BamHI G'GATC_C Cuts at: 2905 3272 2905 Size: 367 5208 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2768 2896 4351 618 Size: 325 217 627 981 128 1455 1842 Fragments arranged by size: 1842 1455 981 627 325 217 128 BanII G_rGCy'C Cuts at: 1391 2894 1391 Size: 1503 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4604 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3766 3800 1903 Size: 34 50 34 1745 34 3678 Fragments arranged by size: 3678 1745 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3467 4677 3467 Size: 1210 4365 BclI T'GATC_A Cuts at: 1548 1548 Size: 5575 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2404 4186 136 Size: 107 122 71 631 605 732 1782 1525 Fragments arranged by size: 1782 1525 732 631 605 122 107 71 BglII A'GATC_T Cuts at: 2479 5570 2479 Size: 3091 2484 BlpI GC'TnA_GC Cuts at: 2992 2992 Size: 5575 Bme1580I G_kGCm'C Cuts at: 1790 3636 4792 1790 Size: 1846 1156 2573 Fragments arranged by size: 2573 1846 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5575 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3306 4264 442 Size: 416 53 83 399 24 1889 958 1753 Fragments arranged by size: 1889 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5575 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2735 4235 1239 Size: 1496 1500 2579 Fragments arranged by size: 2579 1500 1496 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2504 2828 3700 4565 4863 2504 Size: 324 872 865 298 3216 Fragments arranged by size: 3216 872 865 324 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2022 2630 4238 882 Size: 617 523 608 1608 2219 Fragments arranged by size: 2219 1608 617 608 523 BsaAI yAC'GTr Cuts at: 493 1204 3366 493 Size: 711 2162 2702 Fragments arranged by size: 2702 2162 711 BsaBI GATnn'nnATC Cuts at: 5569 5569 Size: 5575 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3382 3764 4399 275 Size: 53 83 186 936 1849 382 635 1451 Fragments arranged by size: 1849 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2796 4008 4406 4680 4827 5238 2796 Size: 1212 398 274 147 411 3133 Fragments arranged by size: 3133 1212 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5350 Fragments arranged by size: 5350 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2846 1186 Size: 1660 3915 BseYI C'CCAG_C Cuts at: 2033 2164 2352 2719 4778 5274 2033 Size: 131 188 367 2059 496 2334 Fragments arranged by size: 2334 2059 496 367 188 131 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5002 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3025 3786 4814 1540 Size: 275 186 1024 761 1028 2301 Fragments arranged by size: 2301 1028 1024 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2788 3636 3721 4792 2788 Size: 848 85 1071 3571 Fragments arranged by size: 3571 1071 848 85 BsmI GAATG_Cn' Cuts at: 3086 3179 3086 Size: 93 5482 BsmBI CGTCTCn'nnnn_ Cuts at: 2017 2017 Size: 5575 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2788 2894 3636 3721 4792 1391 Size: 399 998 106 742 85 1071 2174 Fragments arranged by size: 2174 1071 998 742 399 106 85 BspEI T'CCGG_A Cuts at: 5238 5238 Size: 5575 BspHI T'CATG_A Cuts at: 3464 5194 3464 Size: 1730 3845 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2930 844 Size: 2086 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3462 1394 Size: 429 1639 3507 Fragments arranged by size: 3507 1639 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4070 4244 65 Size: 1183 2822 174 1396 Fragments arranged by size: 2822 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2107 2796 4219 4406 1700 Size: 110 297 689 1423 187 2869 Fragments arranged by size: 2869 1423 689 297 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5575 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5575 BssSI C'ACGA_G Cuts at: 2828 3635 4647 2828 Size: 807 1012 3756 Fragments arranged by size: 3756 1012 807 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5575 BstBI TT'CG_AA Cuts at: 4379 4379 Size: 5575 BstEII G'GTnAC_C Cuts at: 1184 2639 1184 Size: 1455 4120 BstXI CCAn_nnnn'nTGG Cuts at: 2537 2679 2537 Size: 142 5433 BstYI r'GATC_y Cuts at: 1972 2479 2905 3272 3657 3674 5126 5212 5224 Size: 507 426 367 385 17 1452 86 12 Cuts at: 5224 5570 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 507 426 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2620 513 Size: 552 1555 3468 Fragments arranged by size: 3468 1555 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4836 Fragments arranged by size: 4836 683 56 BtsI GCAGTG_nn' Cuts at: 3091 3885 3905 3091 Size: 794 20 4761 Fragments arranged by size: 4761 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5575 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5540 DraI TTT'AAA Cuts at: 2879 2879 Size: 5575 DraIII CAC_nnn'GTG Cuts at: 2662 2662 Size: 5575 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4582 809 Size: 507 3266 1802 Fragments arranged by size: 3266 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3022 3911 5540 Size: 54 1425 50 275 18 1192 889 1629 Cuts at: 5540 8 Size: 43 Fragments arranged by size: 1629 1425 1192 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3022 1537 Size: 275 1210 4090 Fragments arranged by size: 4090 1210 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2306 3505 1353 Size: 953 1199 3423 Fragments arranged by size: 3423 1199 953 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2462 4162 4536 4682 387 Size: 1064 138 873 1700 374 146 1280 Fragments arranged by size: 1700 1280 1064 873 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2279 2673 2735 2799 3638 4235 Size: 393 437 210 394 62 64 839 597 Cuts at: 4235 5022 5290 1239 Size: 787 268 1524 Fragments arranged by size: 1524 839 787 597 437 394 393 268 210 64 62 EcoNI CCTnn'n_nnAGG Cuts at: 2421 2421 Size: 5575 EcoO109I rG'GnC_Cy Cuts at: 2694 2694 Size: 5575 EcoRI G'AATT_C Cuts at: 2884 3262 2884 Size: 378 5197 FspI TGC'GCA Cuts at: 4081 4081 Size: 5575 HaeII r_GCGC'y Cuts at: 1678 2525 4722 1678 Size: 847 2197 2531 Fragments arranged by size: 2531 2197 847 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2049 2081 4216 4248 4290 Size: 32 135 32 477 32 2135 32 42 Cuts at: 4290 4322 1373 Size: 32 2626 Fragments arranged by size: 2626 2135 477 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2919 3165 669 Size: 988 281 981 246 3079 Fragments arranged by size: 3079 988 981 281 246 HindIII A'AGCT_T Cuts at: 748 2237 748 Size: 1489 4086 HpaI GTT'AAC Cuts at: 3165 3165 Size: 5575 KpnI G_GTAC'C Cuts at: 2900 2900 Size: 5575 MfeI C'AATT_G Cuts at: 3174 3174 Size: 5575 MluI A'CGCG_T Cuts at: 5451 5451 Size: 5575 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2080 2580 4689 4873 1305 Size: 775 500 2109 184 2007 Fragments arranged by size: 2109 2007 775 500 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3753 Fragments arranged by size: 3753 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2126 2281 3533 3892 4051 518 Size: 648 960 155 1252 359 159 2042 Fragments arranged by size: 2042 1252 960 648 359 159 155 MspA1I CmG'CkG Cuts at: 1606 2991 3668 4816 5061 1606 Size: 1385 677 1148 245 2120 Fragments arranged by size: 2120 1385 1148 677 245 NaeI GCC'GGC Cuts at: 2109 2109 Size: 5575 NcoI C'CATG_G Cuts at: 513 1065 2620 513 Size: 552 1555 3468 Fragments arranged by size: 3468 1555 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5575 NgoMIV G'CCGG_C Cuts at: 2107 2107 Size: 5575 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5575 NotI GC'GGCC_GC Cuts at: 3022 3022 Size: 5575 NspI r_CATG'y Cuts at: 1132 1229 2142 2507 2933 4478 1132 Size: 97 913 365 426 1545 2229 Fragments arranged by size: 2229 1545 913 426 365 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5575 PciI A'CATG_T Cuts at: 2138 2503 4474 2138 Size: 365 1971 3239 Fragments arranged by size: 3239 1971 365 PflMI CCAn_nnn'nTGG Cuts at: 1108 2124 2662 1108 Size: 1016 538 4021 Fragments arranged by size: 4021 1016 538 PmeI GTTT'AAAC Cuts at: 2879 2879 Size: 5575 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3644 3676 5157 5189 3644 Size: 32 1481 32 4030 Fragments arranged by size: 4030 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2694 2694 Size: 5575 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5575 PsiI TTA'TAA Cuts at: 3145 3145 Size: 5575 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2431 2463 2431 Size: 32 5543 PstI C_TGCA'G Cuts at: 830 2422 2927 830 Size: 1592 505 3478 Fragments arranged by size: 3478 1592 505 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5575 SalI G'TCGA_C Cuts at: 1936 2917 1936 Size: 981 4594 SanDI GG'GwC_CC Cuts at: 2694 2694 Size: 5575 SbfI CC_TGCA'GG Cuts at: 2422 2927 2422 Size: 505 5070 ScaI AGT'ACT Cuts at: 3823 5535 3823 Size: 1712 3863 SfcI C'TryA_G Cuts at: 826 1045 2418 2923 4058 4739 4930 826 Size: 219 1373 505 1135 681 191 1471 Fragments arranged by size: 1471 1373 1135 681 505 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5575 SmaI CCC'GGG Cuts at: 1872 2902 1872 Size: 1030 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2483 2843 3679 4580 4842 820 Size: 197 926 540 360 836 901 262 1553 Fragments arranged by size: 1553 926 901 836 540 360 262 197 SnaBI TAC'GTA Cuts at: 493 3366 493 Size: 2873 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5575 SphI G_CATG'C Cuts at: 1132 2933 1132 Size: 1801 3774 SspI AAT'ATT Cuts at: 5 52 3499 5 Size: 47 3447 2081 Fragments arranged by size: 3447 2081 47 StuI AGG'CCT Cuts at: 1517 2416 1517 Size: 899 4676 StyI C'CwwG_G Cuts at: 513 1065 2620 3014 513 Size: 552 1555 394 3074 Fragments arranged by size: 3074 1555 552 394 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2025 2366 2531 3602 3619 3772 3957 Size: 1255 445 341 165 1071 17 153 185 Cuts at: 3957 4402 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1071 445 445 341 185 165 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2242 2839 3821 Size: 274 80 33 51 1443 265 597 982 Cuts at: 3821 5533 96 Size: 1712 138 Fragments arranged by size: 1712 1443 982 597 274 265 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5535 5567 5535 Size: 32 5543 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5575 XbaI T'CTAG_A Cuts at: 2911 2911 Size: 5575 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2125 2125 Size: 5575 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5575 XmaI C'CCGG_G Cuts at: 1870 2900 1870 Size: 1030 4545 XmnI GAAnn'nnTTC Cuts at: 3704 3704 Size: 5575 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3383 276 Size: 53 83 186 936 1849 2468 Fragments arranged by size: 2468 1849 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI