Restriction map for pFN21AB9436
 (Circular) (Six-base) MAPSORT of: pFN21AB9436.seq  Check: 2199  from: 1  to: 5143



pFN21AB9436  5143 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:43  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2173    2173 
   Size:       5143
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2953     278 
   Size:         53      83     186     936    1417    2468

  Fragments arranged by size: 

                2468    1417     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2486    1937 
   Size:        549    4594
 
Acc65I G'GTAC_C

Cuts at:   2464    2464 
   Size:       5143
 
AclI AA'CG_TT

Cuts at:   1563    3270    3643    1563 
   Size:       1707     373    3063

  Fragments arranged by size: 

                3063    1707     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3206    4590    4858    1632 
   Size:       1574    1384     268    1917

  Fragments arranged by size: 

                1917    1574    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4946
 
AflIII A'CryG_T

Cuts at:   1203    4042    5019    1203 
   Size:       2839     977    1327

  Fragments arranged by size: 

                2839    1327     977
 
AgeI A'CCGG_T

Cuts at:   3974    3974 
   Size:       5143
 
AhdI GACnn_n'nnGTC

Cuts at:   3872    3872 
   Size:       5143
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5143
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5143
 
AlwNI CAG_nnn'CTG

Cuts at:   2074    2263    2356    2886    4458    2074 
   Size:        189      93     530    1572    2759

  Fragments arranged by size: 

                2759    1572     530     189      93
 
ApaLI G'TGCA_C

Cuts at:   3200    4356    3200 
   Size:       1156    3987
 
ApoI r'AATT_y

Cuts at:   1493    2452    2678    2830    4939    1493 
   Size:        959     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     959     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3697     160 
   Size:        872    2665    1606

  Fragments arranged by size: 

                2665    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5143
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2468    1870 
   Size:         73     525    4545

  Fragments arranged by size: 

                4545     525      73
 
BamHI G'GATC_C

Cuts at:   2473    2840    2473 
   Size:        367    4776
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2146    2464    3919     618 
   Size:        325     217     627     359     318    1455    1842

  Fragments arranged by size: 

                1842    1455     627     359     325     318     217
 
BanII G_rGCy'C

Cuts at:   1391    2336    2462    1391 
   Size:        945     126    4072

  Fragments arranged by size: 

                4072     945     126
 
BbeI G_GCGC'C

Cuts at:   2150    2150 
   Size:       5143
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4172
 
BbvCI CC'TCA_GC

Cuts at:   2103    2103 
   Size:       5143
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3334    3368    1903 
   Size:         34    1397      34    3678

  Fragments arranged by size: 

                3678    1397      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3035    4245    3035 
   Size:       1210    3933
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5143
 
BfrBI ATG'CAT

Cuts at:   2229    2229 
   Size:       5143
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3754     136 
   Size:        107     122      71     631     605    2082    1525

  Fragments arranged by size: 

                2082    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5138    5138 
   Size:       5143
 
BlpI GC'TnA_GC

Cuts at:   2560    2560 
   Size:       5143
 
Bme1580I G_kGCm'C

Cuts at:   1790    3204    4360    1790 
   Size:       1414    1156    2573

  Fragments arranged by size: 

                2573    1414    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5143
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2874    3832     442 
   Size:        416      53      83     399      24    1457     958    1753

  Fragments arranged by size: 

                1753    1457     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5143
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2209    2209 
   Size:       5143
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3803    1239 
   Size:       2564    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2103    2330    2103 
   Size:        227    4916
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3268    4133    4431    3268 
   Size:        865     298    3980

  Fragments arranged by size: 

                3980     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3806     882 
   Size:        617    2307    2219

  Fragments arranged by size: 

                2307    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2934     493 
   Size:        711    1730    2702

  Fragments arranged by size: 

                2702    1730     711
 
BsaBI GATnn'nnATC

Cuts at:   5137    5137 
   Size:       5143
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2147    2950    3332    3967 
   Size:         53      83     186     936     614     803     382     635

Cuts at:   3967     275 
   Size:       1451

  Fragments arranged by size: 

                1451     936     803     635     614     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3576    3974    4248    4395    4806    3576 
   Size:        398     274     147     411    3913

  Fragments arranged by size: 

                3913     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4918

  Fragments arranged by size: 

                4918     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2091    1186 
   Size:        905    4238
 
BseYI C'CCAG_C

Cuts at:   2015    4346    4842    2015 
   Size:       2331     496    2316

  Fragments arranged by size: 

                2331    2316     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4570
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2593    3354    4382    1540 
   Size:        275     186     592     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     592     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3204    3289    4360    3204 
   Size:         85    1071    3987

  Fragments arranged by size: 

                3987    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2654    2747    2654 
   Size:         93    5050
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2336    2462    3204    3289    4360    1391 
   Size:        399     546     126     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     546     399     126      85
 
BspEI T'CCGG_A

Cuts at:   4806    4806 
   Size:       5143
 
BspHI T'CATG_A

Cuts at:   3032    4762    3032 
   Size:       1730    3413
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2173    2498     844 
   Size:       1329     325    3489

  Fragments arranged by size: 

                3489    1329     325
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3030    1394 
   Size:        429    1207    3507

  Fragments arranged by size: 

                3507    1207     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3638    3812      65 
   Size:       1183    2390     174    1396

  Fragments arranged by size: 

                2390    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2113    2143    3787    3974    1700 
   Size:        110     303      30    1644     187    2869

  Fragments arranged by size: 

                2869    1644     303     187     110      30
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5143
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5143
 
BssSI C'ACGA_G

Cuts at:   3203    4215    3203 
   Size:       1012    4131
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2356     794 
   Size:       1562    3581
 
BstBI TT'CG_AA

Cuts at:   2427    3947    2427 
   Size:       1520    3623
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5143
 
BstYI r'GATC_y

Cuts at:   1972    2473    2840    3225    3242    4694    4780    4792    5138 
   Size:        501     367     385      17    1452      86      12     346

Cuts at:   5138    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     501     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2150     513 
   Size:        552     939     146    3506

  Fragments arranged by size: 

                3506     939     552     146
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4404

  Fragments arranged by size: 

                4404     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2659    3453    3473    2659 
   Size:        794      20    4329

  Fragments arranged by size: 

                4329     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5143
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5108
 
DraI TTT'AAA

Cuts at:   2447    2447 
   Size:       5143
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4150     809 
   Size:        507    2834    1802

  Fragments arranged by size: 

                2834    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2590    3479    5108 
   Size:         54    1425      50     275      18     760     889    1629

Cuts at:   5108       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     760     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2590    1537 
   Size:        275     778    4090

  Fragments arranged by size: 

                4090     778     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2178    3073    1353 
   Size:        825     895    3423

  Fragments arranged by size: 

                3423     895     825
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2054    3730    4104    4250     387 
   Size:       1064     138     465    1676     374     146    1280

  Fragments arranged by size: 

                1676    1280    1064     465     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3206    3803    4590    4858    1239 
   Size:        393    1574     597     787     268    1524

  Fragments arranged by size: 

                1574    1524     787     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2260    2260 
   Size:       5143
 
EcoRI G'AATT_C

Cuts at:   2452    2830    2452 
   Size:        378    4765
 
FspI TGC'GCA

Cuts at:   2297    3649    2297 
   Size:       1352    3791
 
HaeII r_GCGC'y

Cuts at:   1678    2150    4290    1678 
   Size:        472    2140    2531

  Fragments arranged by size: 

                2531    2140     472
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3784    3816    3858    3890    1373 
   Size:         32     135      32    2212      32      42      32    2626

  Fragments arranged by size: 

                2626    2212     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2487    2733     669 
   Size:        988     281     549     246    3079

  Fragments arranged by size: 

                3079     988     549     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5143
 
HpaI GTT'AAC

Cuts at:   2733    2733 
   Size:       5143
 
KasI G'GCGC_C

Cuts at:   2146    2146 
   Size:       5143
 
KpnI G_GTAC'C

Cuts at:   2468    2468 
   Size:       5143
 
MfeI C'AATT_G

Cuts at:   2742    2742 
   Size:       5143
 
MluI A'CGCG_T

Cuts at:   5019    5019 
   Size:       5143
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2088    4257    4441    1305 
   Size:        783    2169     184    2007

  Fragments arranged by size: 

                2169    2007     783     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3321

  Fragments arranged by size: 

                3321    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3101    3460    3619     518 
   Size:        648    1935     359     159    2042

  Fragments arranged by size: 

                2042    1935     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2074    2092    2356    2559    3236    4384    4629    1606 
   Size:        468      18     264     203     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     468     264     245     203      18
            
 
NaeI GCC'GGC

Cuts at:   2145    2145 
   Size:       5143
 
NarI GG'CG_CC

Cuts at:   2147    2147 
   Size:       5143
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3652

  Fragments arranged by size: 

                3652     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5143
 
NgoMIV G'CCGG_C

Cuts at:   2143    2143 
   Size:       5143
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5143
 
NotI GC'GGCC_GC

Cuts at:   2590    2590 
   Size:       5143
 
NsiI A_TGCA'T

Cuts at:   2231    2231 
   Size:       5143
 
NspI r_CATG'y

Cuts at:   1132    1229    2425    2501    4046    1132 
   Size:         97    1196      76    1545    2229

  Fragments arranged by size: 

                2229    1545    1196      97      76
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5143
 
PciI A'CATG_T

Cuts at:   4042    4042 
   Size:       5143
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5143
 
PfoI T'CCnGG_A

Cuts at:   2286    2367    2286 
   Size:         81    5062
 
PmeI GTTT'AAAC

Cuts at:   2447    2447 
   Size:       5143
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3212    3244    4725    4757    3212 
   Size:         32    1481      32    3598

  Fragments arranged by size: 

                3598    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2260    2260 
   Size:       5143
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5143
 
PsiI TTA'TAA

Cuts at:   2713    2713 
   Size:       5143
 
PstI C_TGCA'G

Cuts at:    830    2361    2495     830 
   Size:       1531     134    3478

  Fragments arranged by size: 

                3478    1531     134
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5143
 
PvuII CAG'CTG

Cuts at:   2356    2356 
   Size:       5143
 
SalI G'TCGA_C

Cuts at:   1936    2485    1936 
   Size:        549    4594
 
SbfI CC_TGCA'GG

Cuts at:   2495    2495 
   Size:       5143
 
ScaI AGT'ACT

Cuts at:   3391    5103    3391 
   Size:       1712    3431
 
SfcI C'TryA_G

Cuts at:    826    1045    2357    2491    3626    4307    4498     826 
   Size:        219    1312     134    1135     681     191    1471

  Fragments arranged by size: 

                1471    1312    1135     681     219     191     134
 
SfoI GGC'GCC

Cuts at:   2148    2148 
   Size:       5143
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5143
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    2143    1700 
   Size:        443    4700
 
SmaI CCC'GGG

Cuts at:   1872    2470    1872 
   Size:        598    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3247    4148    4410     820 
   Size:        197     926    1304     901     262    1553

  Fragments arranged by size: 

                1553    1304     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2934     493 
   Size:       2441    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5143
 
SphI G_CATG'C

Cuts at:   1132    2501    1132 
   Size:       1369    3774
 
SspI AAT'ATT

Cuts at:      5      52    3067       5 
   Size:         47    3015    2081

  Fragments arranged by size: 

                3015    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5143
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2436    2582     513 
   Size:        552     939     432     146    3074

  Fragments arranged by size: 

                3074     939     552     432     146
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3170    3187    3340    3525    3970     325 
   Size:       1255    1590      17     153     185     445    1498

  Fragments arranged by size: 

                1590    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2406    3389    5101 
   Size:        274      80      33      51    1443     429     983    1712

Cuts at:   5101      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443     983     429     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5103    5135    5103 
   Size:         32    5111
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5143
 
XbaI T'CTAG_A

Cuts at:   2479    2479 
   Size:       5143
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5143
 
XmaI C'CCGG_G

Cuts at:   1870    2468    1870 
   Size:        598    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3272    3272 
   Size:       5143
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2951     276 
   Size:         53      83     186     936    1417    2468

  Fragments arranged by size: 

                2468    1417     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NsiI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI
     PvuI    PvuII     SalI     SbfI     ScaI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BsiWI    BsmBI
    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI    EcoRV     FalI
     FseI    FspAI     NruI     PacI     PmlI   PspOMI    PspXI     PsrI
    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI     SrfI
     SwaI     XcmI
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