Restriction map for
pFN21AB9171
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# NO CUT ENZYMES ( 98 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BbrPI BbsI BbvCI
BcgI BclI BfrI BglII BlpI
Bpu1102I BpuAI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrGI Bst1107I BstBI BstXI
BstZ17I CelII ClaI CpoI DraI
Eam1105I Eco47III EcoRI EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MluI MseI
MunI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 259 TCCGGA T^CCGGA
AgeI 1654 ACCGGT A^CCGGT
ApaI 1758 GGGCCC GGGCC^C
BbeI 999 GGCGCC GGCGC^C
BciVI 205 GTATCC GTATCC(6/5)
BsaAI 712 TACGTG [CT]AC^GT[GA]
BseAI 259 TCCGGA T^CCGGA
BsiEI 882 CGGCCG CG[GA][CT]^CG
BspEI 259 TCCGGA T^CCGGA
BsrDI 1783 GCAATG GCAATG(2/0)
BssHII 305 GCGCGC G^CGCGC
BssSI 1342 CACGAG CACGAG(-5/-1)
BstAPI 729 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 967 GGTGACC G^GT[ACGT]ACC
BstPI 967 GGTGACC G^GT[ACGT]ACC
DraIII 1996 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 556 GACCCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 882 CGGCCG C^GGCCG
EclXI 882 CGGCCG C^GGCCG
Eco52I 882 CGGCCG C^GGCCG
EcoO65I 967 GGTGACC G^GT[ACGT]ACC
EcoRV 1258 GATATC GAT^ATC
HincII 670 GTCAAC GT[CT]^[GA]AC
HindII 670 GTCAAC GT[CT]^[GA]AC
KasI 999 GGCGCC G^GCGCC
MroI 259 TCCGGA T^CCGGA
MslI 583 CACCGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 877 GCCGGC GCC^GGC
NarI 999 GGCGCC GG^CGCC
NgoMIV 877 GCCGGC G^CCGGC
NheI 1848 GCTAGC G^CTAGC
NspI 465 ACATGC [GA]CATG^[CT]
PinAI 1654 ACCGGT A^CCGGT
PshAI 222 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1185 GAGCTC GAGCT^C
SapI 1062 GAAGAGC GCTCTTC(1/4)
SbfI 1752 CCTGCAGG CCTGCA^GG
SfiI 1750 GGCCTGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 999 GGCGCC GGC^GCC
Sse8387I 1752 CCTGCAGG CCTGCA^GG
XcmI 1466 CCACCCTGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AatII 1282 GACGTC GACGT^C
1927 GACGTC
Alw44I 187 GTGCAC G^TGCAC
517 GTGCAC
AocI 811 CCTGAGG CC^T[ACGT]AGG
1308 CCTGAGG
ApaLI 187 GTGCAC G^TGCAC
517 GTGCAC
AvrII 231 CCTAGG C^CTAGG
1896 CCTAGG
BlnI 231 CCTAGG C^CTAGG
1896 CCTAGG
BsaI 220 GAGACC GGTCTC(1/5)
777 GGTCTC
BsaWI 259 TCCGGA [AT]^CCGG[AT]
1654 ACCGGT
Bsu36I 811 CCTGAGG CC^T[ACGT]AGG
1308 CCTGAGG
EarI 169 GAAGAG CTCTTC(1/4)
1062 GAAGAG
Eco81I 811 CCTGAGG CC^T[ACGT]AGG
1308 CCTGAGG
Ksp632I 169 GAAGAG CTCTTC(1/4)
1062 GAAGAG
PpuMI 227 AGGTCCT [GA]G^G[AT]CC[CT]
806 GGGACCC
TaqI 74 TCGA T^CGA
269 TCGA
TthHB8I 74 TCGA T^CGA
269 TCGA
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BamHI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI StuI StyI TseI Tsp45I
TspRI XhoII XmaCI XmaI