Restriction map for
pFN21AB9038
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BbrPI BbvCI
BciVI BcnI BfrI BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoT22I FseI FspI HgaI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI Tsp509I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcyI 325 GGCGCC G[GA]^CG[CT]C
Alw44I 13 GTGCAC G^TGCAC
AlwNI 428 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 13 GTGCAC G^TGCAC
AspI 1249 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1031 GGATCC G^GATCC
BbeI 325 GGCGCC GGCGC^C
BcgI 38 GCAACCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 68 TGATCA T^GATCA
BfaI 202 CTAG C^TAG
BglI 386 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 248 AGATCT A^GATCT
BsaHI 325 GGCGCC G[GA]^CG[CT]C
BsaI 592 GAGACC GGTCTC(1/5)
BsgI 997 GTGCAG GTGCAG(16/14)
BsiEI 921 CGGCCG CG[GA][CT]^CG
BspHI 488 TCATGA T^CATGA
BsrFI 97 ACCGGC [GA]^CCGG[CT]
BstXI 188 CCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 97 ACCGGC [GA]^CCGG[CT]
DraIII 1070 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 529 GACACCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 921 CGGCCG C^GGCCG
EclXI 921 CGGCCG C^GGCCG
Eco52I 921 CGGCCG C^GGCCG
EcoRV 625 GATATC GAT^ATC
EcoT14I 215 CCATGG C^C[AT][AT]GG
FbaI 68 TGATCA T^GATCA
HaeII 325 GGCGCC [GA]GCGC^[CT]
Hin1I 325 GGCGCC G[GA]^CG[CT]C
HincII 763 GTCAAC GT[CT]^[GA]AC
HindII 763 GTCAAC GT[CT]^[GA]AC
KasI 325 GGCGCC G^GCGCC
MaeI 202 CTAG C^TAG
MseI 992 TTAA T^TAA
NarI 325 GGCGCC GG^CGCC
NcoI 215 CCATGG C^CATGG
PflFI 1249 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 220 GACTC GAGTC(4/5)
PpuMI 234 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1050 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 488 TCATGA T^CATGA
SapI 1128 GCTCTTC GCTCTTC(1/4)
SbfI 387 CCTGCAGG CCTGCA^GG
SfoI 325 GGCGCC GGC^GCC
SmlI 1081 CTCAAG C^T[CT][GA]AG
Sse8387I 387 CCTGCAGG CCTGCA^GG
StuI 1175 AGGCCT AGG^CCT
StyI 215 CCATGG C^C[AT][AT]GG
TfiI 340 GATTC G^A[AT]TC
Tru9I 992 TTAA T^TAA
Tth111I 1249 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 201 TCTAGA T^CTAGA
XcmI 887 CCAAGAACATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Asp700I 492 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1173 GAAGGCCTTC
BalI 243 TGGCCA TGG^CCA
729 TGGCCA
BbsI 877 GTCTTC GAAGAC(2/6)
946 GTCTTC
BpuAI 877 GTCTTC GAAGAC(2/6)
946 GTCTTC
BsmBI 784 CGTCTC CGTCTC(1/5)
1254 CGTCTC
BssSI 182 CTCGTG CACGAG(-5/-1)
1073 CTCGTG
CfoI 326 GCGC GCG^C
1137 GCGC
DraII 234 AGGTCCT [GA]G^G[ACGT]CC[CT]
614 AGGCCCT
EcoO109I 234 AGGTCCT [GA]G^G[ACGT]CC[CT]
614 AGGCCCT
FauI 914 CCCGC CCCGC(4/6)
419 GCGGG
HhaI 326 GCGC GCG^C
1137 GCGC
HinP1I 326 GCGC G^CGC
1137 GCGC
HinfI 220 GACTC G^A[ACGT]TC
340 GATTC
MaeII 783 ACGT A^CGT
995 ACGT
MluNI 243 TGGCCA TGG^CCA
729 TGGCCA
MscI 243 TGGCCA TGG^CCA
729 TGGCCA
MspA1I 127 CAGCTG C[AC]G^C[GT]G
400 CAGCTG
PstI 124 CTGCAG CTGCA^G
388 CTGCAG
PvuII 127 CAGCTG CAG^CTG
400 CAGCTG
SacI 845 GAGCTC GAGCT^C
1126 GAGCTC
SfcI 124 CTGCAG C^T[GA][CT]AG
388 CTGCAG
XmnI 492 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1173 GAAGGCCTTC
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BanII BbvI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PflMI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XhoII