(Circular) (Six-base) MAPSORT of: pFN21AB9038.seq Check: 4674 from: 1 to: 6037 pFN21AB9038 6037 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2726 2726 Size: 6037 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3847 278 Size: 53 83 186 936 2311 2468 Fragments arranged by size: 2468 2311 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3380 1937 Size: 1443 4594 Acc65I G'GTAC_C Cuts at: 3358 3358 Size: 6037 AclI AA'CG_TT Cuts at: 1563 4164 4537 1563 Size: 2601 373 3063 Fragments arranged by size: 3063 2601 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2488 2669 4100 5484 5752 1632 Size: 856 181 1431 1384 268 1917 Fragments arranged by size: 1917 1431 1384 856 268 181 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5840 AflIII A'CryG_T Cuts at: 1203 4936 5913 1203 Size: 3733 977 1327 Fragments arranged by size: 3733 1327 977 AgeI A'CCGG_T Cuts at: 4868 4868 Size: 6037 AhdI GACnn_n'nnGTC Cuts at: 4766 4766 Size: 6037 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6037 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3177 1184 Size: 1993 4044 AlwNI CAG_nnn'CTG Cuts at: 2438 3780 5352 2438 Size: 1342 1572 3123 Fragments arranged by size: 3123 1572 1342 ApaLI G'TGCA_C Cuts at: 2018 4094 5250 2018 Size: 2076 1156 2805 Fragments arranged by size: 2805 2076 1156 ApoI r'AATT_y Cuts at: 1493 3346 3572 3724 5833 1493 Size: 1853 226 152 2109 1697 Fragments arranged by size: 2109 1853 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4591 160 Size: 872 3559 1606 Fragments arranged by size: 3559 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 AvaI C'yCGr_G Cuts at: 1870 1943 3362 1870 Size: 73 1419 4545 Fragments arranged by size: 4545 1419 73 BamHI G'GATC_C Cuts at: 3036 3367 3734 3036 Size: 331 367 5339 Fragments arranged by size: 5339 367 331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2105 2330 2512 3358 Size: 325 217 627 267 51 225 182 846 Cuts at: 3358 4813 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 846 627 325 267 225 217 182 51 BanII G_rGCy'C Cuts at: 1391 2550 2854 3135 3356 1391 Size: 1159 304 281 221 4072 Fragments arranged by size: 4072 1159 304 281 221 BbeI G_GCGC'C Cuts at: 2334 2334 Size: 6037 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2875 2944 928 Size: 971 976 69 4021 Fragments arranged by size: 4021 976 971 69 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2032 2066 4228 4262 1903 Size: 34 95 34 2162 34 3678 Fragments arranged by size: 3678 2162 95 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3929 5139 3929 Size: 1210 4827 BclI T'GATC_A Cuts at: 1548 2073 1548 Size: 525 5512 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2397 4648 136 Size: 107 122 71 631 605 725 2251 1525 Fragments arranged by size: 2251 1525 725 631 605 122 107 71 BglII A'GATC_T Cuts at: 2253 6032 2253 Size: 3779 2258 BlpI GC'TnA_GC Cuts at: 3454 3454 Size: 6037 Bme1580I G_kGCm'C Cuts at: 1790 2022 2432 2450 4098 5254 1790 Size: 232 410 18 1648 1156 2573 Fragments arranged by size: 2573 1648 1156 410 232 18 BmgBI CAC'GTC Cuts at: 1318 2789 1318 Size: 1471 4566 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 2429 3768 Size: 416 53 83 399 24 897 115 1339 Cuts at: 3768 4726 442 Size: 958 1753 Fragments arranged by size: 1753 1339 958 897 416 399 115 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6037 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4697 1239 Size: 3458 2579 Bpu10I CC'TnA_GC Cuts at: 2236 2236 Size: 6037 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3071 4162 5027 5325 3071 Size: 1091 865 298 3783 Fragments arranged by size: 3783 1091 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2591 4700 882 Size: 617 1092 2109 2219 Fragments arranged by size: 2219 2109 1092 617 BsaAI yAC'GTr Cuts at: 493 1204 3828 493 Size: 711 2624 2702 Fragments arranged by size: 2702 2624 711 BsaBI GATnn'nnATC Cuts at: 6031 6031 Size: 6037 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2331 3844 4226 4861 Size: 53 83 186 936 798 1513 382 635 Cuts at: 4861 275 Size: 1451 Fragments arranged by size: 1513 1451 936 798 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4470 4868 5142 5289 5700 4470 Size: 398 274 147 411 4807 Fragments arranged by size: 4807 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5812 Fragments arranged by size: 5812 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3314 3317 3332 3344 1186 Size: 2128 3 15 12 3879 Fragments arranged by size: 3879 2128 15 12 3 BseYI C'CCAG_C Cuts at: 2874 5240 5736 2874 Size: 2366 496 3175 Fragments arranged by size: 3175 2366 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3023 1163 Size: 573 1287 4177 Fragments arranged by size: 4177 1287 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2929 3487 4248 5276 1540 Size: 275 186 928 558 761 1028 2301 Fragments arranged by size: 2301 1028 928 761 558 275 186 BsiHKAI G_wGCw'C Cuts at: 2022 2854 3135 4098 4183 5254 2022 Size: 832 281 963 85 1071 2805 Fragments arranged by size: 2805 1071 963 832 281 85 BsmI GAATG_Cn' Cuts at: 3548 3641 3548 Size: 93 5944 BsmBI CGTCTCn'nnnn_ Cuts at: 2795 3265 2795 Size: 470 5567 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2432 2450 2550 2854 3135 3356 Size: 399 232 410 18 100 304 281 221 Cuts at: 3356 4098 4183 5254 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 410 399 304 281 232 221 100 85 18 BspEI T'CCGG_A Cuts at: 5700 5700 Size: 6037 BspHI T'CATG_A Cuts at: 2493 3926 5656 2493 Size: 1433 1730 2874 Fragments arranged by size: 2874 1730 1433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2136 2285 2726 2869 3392 844 Size: 1292 149 441 143 523 3489 Fragments arranged by size: 3489 1292 523 441 149 143 BsrBI CCG'CTC Cuts at: 1394 1823 3924 1394 Size: 429 2101 3507 Fragments arranged by size: 3507 2101 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4532 4706 65 Size: 1183 3284 174 1396 Fragments arranged by size: 3284 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2102 4681 4868 1700 Size: 110 292 2579 187 2869 Fragments arranged by size: 2869 2579 292 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6037 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6037 BssSI C'ACGA_G Cuts at: 2187 3078 4097 5109 2187 Size: 891 1019 1012 3115 Fragments arranged by size: 3115 1019 1012 891 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6037 BstBI TT'CG_AA Cuts at: 4841 4841 Size: 6037 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6037 BstXI CCAn_nnnn'nTGG Cuts at: 2200 2200 Size: 6037 BstYI r'GATC_y Cuts at: 1972 2203 2253 2466 2674 3036 3367 3734 4119 Size: 231 50 213 208 362 331 367 385 Cuts at: 4119 4136 5588 5674 5686 6032 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 362 346 331 231 213 208 86 50 17 12 BtgI C'CryG_G Cuts at: 513 1065 2113 2220 2538 2830 2923 2940 2956 Size: 552 1048 107 318 292 93 17 16 Cuts at: 2956 513 Size: 3594 Fragments arranged by size: 3594 1048 552 318 292 107 93 17 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5298 Fragments arranged by size: 5298 683 56 BtsI GCAGTG_nn' Cuts at: 3159 3553 4347 4367 3159 Size: 394 794 20 4829 Fragments arranged by size: 4829 794 394 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6037 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6002 DraI TTT'AAA Cuts at: 3341 3341 Size: 6037 DraIII CAC_nnn'GTG Cuts at: 3080 3080 Size: 6037 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2540 5044 809 Size: 507 1224 2504 1802 Fragments arranged by size: 2504 1802 1224 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2248 2734 2926 Size: 54 1425 50 275 18 418 486 192 Cuts at: 2926 3484 4373 6002 8 Size: 558 889 1629 43 Fragments arranged by size: 1629 1425 889 558 486 418 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2926 3484 1537 Size: 275 1114 558 4090 Fragments arranged by size: 4090 1114 558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3140 3291 3321 3967 1353 Size: 1787 151 30 646 3423 Fragments arranged by size: 3423 1787 646 151 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3161 4624 4998 5144 387 Size: 1064 138 1572 1463 374 146 1280 Fragments arranged by size: 1572 1463 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2852 3133 2852 Size: 281 5756 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2488 2669 4100 4697 5484 5752 1239 Size: 393 856 181 1431 597 787 268 1524 Fragments arranged by size: 1524 1431 856 787 597 393 268 181 EcoO109I rG'GnC_Cy Cuts at: 2240 2620 2240 Size: 380 5657 EcoRI G'AATT_C Cuts at: 3346 3724 3346 Size: 378 5659 EcoRV GAT'ATC Cuts at: 2632 2632 Size: 6037 FspI TGC'GCA Cuts at: 4543 4543 Size: 6037 HaeII r_GCGC'y Cuts at: 1678 2334 5184 1678 Size: 656 2850 2531 Fragments arranged by size: 2850 2531 656 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2129 2161 3245 3277 4678 Size: 32 135 32 557 32 1084 32 1401 Cuts at: 4678 4710 4752 4784 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1401 1084 557 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2770 3381 3627 669 Size: 988 281 832 611 246 3079 Fragments arranged by size: 3079 988 832 611 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6037 HpaI GTT'AAC Cuts at: 3627 3627 Size: 6037 KasI G'GCGC_C Cuts at: 2330 2330 Size: 6037 KpnI G_GTAC'C Cuts at: 3362 3362 Size: 6037 MfeI C'AATT_G Cuts at: 3636 3636 Size: 6037 MluI A'CGCG_T Cuts at: 5913 5913 Size: 6037 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2942 5151 5335 1305 Size: 1637 2209 184 2007 Fragments arranged by size: 2209 2007 1637 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2250 2736 10 Size: 54 1425 343 418 486 3311 Fragments arranged by size: 3311 1425 486 418 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2198 3164 3995 4354 4513 518 Size: 648 981 51 966 831 359 159 2042 Fragments arranged by size: 2042 981 966 831 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2134 2407 3453 4130 5278 5523 1606 Size: 528 273 1046 677 1148 245 2120 Fragments arranged by size: 2120 1148 1046 677 528 273 245 NarI GG'CG_CC Cuts at: 2331 2331 Size: 6037 NcoI C'CATG_G Cuts at: 513 1065 2220 513 Size: 552 1155 4330 Fragments arranged by size: 4330 1155 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6037 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6037 NotI GC'GGCC_GC Cuts at: 3484 3484 Size: 6037 NspI r_CATG'y Cuts at: 1132 1229 3395 4940 1132 Size: 97 2166 1545 2229 Fragments arranged by size: 2229 2166 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6037 PciI A'CATG_T Cuts at: 4936 4936 Size: 6037 PflMI CCAn_nnn'nTGG Cuts at: 1108 2064 2419 2578 3080 1108 Size: 956 355 159 502 4065 Fragments arranged by size: 4065 956 502 355 159 PfoI T'CCnGG_A Cuts at: 2352 3126 2352 Size: 774 5263 PmeI GTTT'AAAC Cuts at: 3341 3341 Size: 6037 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3209 3241 4106 4138 5619 5651 3209 Size: 32 865 32 1481 32 3595 Fragments arranged by size: 3595 1481 865 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2240 2240 Size: 6037 PshAI GACnn'nnGTC Cuts at: 1613 3059 1613 Size: 1446 4591 PsiI TTA'TAA Cuts at: 3607 3607 Size: 6037 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3005 3037 3005 Size: 32 6005 PstI C_TGCA'G Cuts at: 830 2133 2397 3389 830 Size: 1303 264 992 3478 Fragments arranged by size: 3478 1303 992 264 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6037 PvuII CAG'CTG Cuts at: 2134 2407 2134 Size: 273 5764 SacI G_AGCT'C Cuts at: 2854 3135 2854 Size: 281 5756 SalI G'TCGA_C Cuts at: 1936 3379 1936 Size: 1443 4594 SapI GCTCTTCn'nnn_ Cuts at: 3140 3140 Size: 6037 SbfI CC_TGCA'GG Cuts at: 2397 3389 2397 Size: 992 5045 ScaI AGT'ACT Cuts at: 4285 5997 4285 Size: 1712 4325 SfcI C'TryA_G Cuts at: 826 1045 2129 2393 3385 4520 5201 5392 826 Size: 219 1084 264 992 1135 681 191 1471 Fragments arranged by size: 1471 1135 1084 992 681 264 219 191 SfoI GGC'GCC Cuts at: 2332 2332 Size: 6037 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6037 SmaI CCC'GGG Cuts at: 1872 3364 1872 Size: 1492 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3086 4141 5042 5304 820 Size: 197 926 1143 1055 901 262 1553 Fragments arranged by size: 1553 1143 1055 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3828 493 Size: 3335 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6037 SphI G_CATG'C Cuts at: 1132 3395 1132 Size: 2263 3774 SspI AAT'ATT Cuts at: 5 52 3961 5 Size: 47 3909 2081 Fragments arranged by size: 3909 2081 47 StuI AGG'CCT Cuts at: 1517 3182 1517 Size: 1665 4372 StyI C'CwwG_G Cuts at: 513 1065 2220 3476 513 Size: 552 1155 1256 3074 Fragments arranged by size: 3074 1256 1155 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2536 2888 4064 4081 4234 4419 4864 Size: 1255 956 352 1176 17 153 185 445 Cuts at: 4864 325 Size: 1498 Fragments arranged by size: 1498 1255 1176 956 445 352 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2151 4283 5995 Size: 274 80 33 51 1443 174 2132 1712 Cuts at: 5995 96 Size: 138 Fragments arranged by size: 2132 1712 1443 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5997 6029 5997 Size: 32 6005 Tth111I GACn'n_nGTC Cuts at: 1316 3257 1316 Size: 1941 4096 XbaI T'CTAG_A Cuts at: 2206 3373 2206 Size: 1167 4870 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2899 2899 Size: 6037 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6037 XmaI C'CCGG_G Cuts at: 1870 3362 1870 Size: 1492 4545 XmnI GAAnn'nnTTC Cuts at: 2501 3182 4166 2501 Size: 681 984 4372 Fragments arranged by size: 4372 984 681 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3845 276 Size: 53 83 186 936 2311 2468 Fragments arranged by size: 2468 2311 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI