Restriction map for
pFN21AB8999
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BbvCI
BciVI BclI BfrI BglI BlnI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI DraI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII SacII SalI SbfI ScaI
SfcI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tsp509I TspRI Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflIII 818 ACACGT A^C[GA][CT]GT
Alw44I 295 GTGCAC G^TGCAC
AlwNI 381 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 295 GTGCAC G^TGCAC
BamHI 893 GGATCC G^GATCC
BbrPI 819 CACGTG CAC^GTG
BcgI 321 GCAGCTAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 308 CCGGG CC^[GC]GG
BglII 665 AGATCT A^GATCT
BlpI 285 GCTGAGC GC^T[ACGT]AGC
BpmI 696 CTCCAG CTGGAG(16/14)
Bpu1102I 285 GCTGAGC GC^T[ACGT]AGC
BsaAI 819 CACGTG [CT]AC^GT[GA]
BsaI 55 GAGACC GGTCTC(1/5)
BseRI 568 GAGGAG GAGGAG(10/8)
BsgI 372 CTGCAC GTGCAG(16/14)
BspHI 479 TCATGA T^CATGA
BsrBI 634 CCGCTC CCGCTC(-3/-3)
BssSI 298 CACGAG CACGAG(-5/-1)
BstXI 401 CCATAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 285 GCTGAGC GC^T[ACGT]AGC
CpoI 779 CGGTCCG CG^G[AT]CCG
DraIII 214 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 242 CCATGG C^C[GA][CT]GG
EarI 649 CTCTTC CTCTTC(1/4)
EcoT14I 242 CCATGG C^C[AT][AT]GG
HapII 308 CCGG C^CGG
HinfI 748 GACTC G^A[ACGT]TC
HpaII 308 CCGG C^CGG
Ksp632I 649 CTCTTC CTCTTC(1/4)
MseI 998 TTAA T^TAA
MslI 870 CATTGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 308 CCGG C^CGG
NciI 308 CCGGG CC^[GC]GG
NcoI 242 CCATGG C^CATGG
PleI 748 GACTC GAGTC(4/5)
PmaCI 819 CACGTG CAC^GTG
PmlI 819 CACGTG CAC^GTG
RcaI 479 TCATGA T^CATGA
RsrII 779 CGGTCCG CG^G[AT]CCG
SacI 913 GAGCTC GAGCT^C
SapI 648 GCTCTTC GCTCTTC(1/4)
SexAI 230 ACCTGGT A^CC[AT]GGT
StyI 242 CCATGG C^C[AT][AT]GG
Tru9I 998 TTAA T^TAA
XcmI 680 CCAAGTTCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 311 GGCGCC G[GA]^CG[CT]C
619 GGCGCC
BanI 311 GGCGCC G^G[CT][GA]CC
619 GGCGCC
BanII 693 GGGCTC G[GA]GC[CT]^C
913 GAGCTC
BbeI 311 GGCGCC GGCGC^C
619 GGCGCC
BbsI 702 GAAGAC GAAGAC(2/6)
112 GTCTTC
BfaI 325 CTAG C^TAG
1061 CTAG
BpuAI 702 GAAGAC GAAGAC(2/6)
112 GTCTTC
BsaHI 311 GGCGCC G[GA]^CG[CT]C
619 GGCGCC
BsmFI 815 GGGAC GGGAC(10/14)
923 GTCCC
BsrI 419 ACTGG ACTGG(1/-1)
659 ACTGG
BstYI 665 AGATCT [GA]^GATC[CT]
893 GGATCC
DdeI 286 CTGAG C^T[ACGT]AG
557 CTGAG
HgaI 467 GCGTC GACGC(5/10)
713 GCGTC
Hin1I 311 GGCGCC G[GA]^CG[CT]C
619 GGCGCC
KasI 311 GGCGCC G^GCGCC
619 GGCGCC
MaeI 325 CTAG C^TAG
1061 CTAG
MflI 665 AGATCT [GA]^GATC[CT]
893 GGATCC
NarI 311 GGCGCC GG^CGCC
619 GGCGCC
PflMI 225 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
380 CCAGCATCTGG
SfoI 311 GGCGCC GGC^GCC
619 GGCGCC
Van91I 225 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
380 CCAGCATCTGG
XhoII 665 AGATCT [GA]^GATC[CT]
893 GGATCC
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BbvI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmBI
Bsp1286I BspMI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MvaI MvnI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp45I