(Circular) (Six-base) MAPSORT of: pFN21AB8999.seq Check: 1334 from: 1 to: 5776 pFN21AB8999 5776 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3839 5223 5491 1632 Size: 2207 1384 268 1917 Fragments arranged by size: 2207 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 2823 3073 4675 5652 1203 Size: 1620 250 1602 977 1327 Fragments arranged by size: 1620 1602 1327 977 250 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2012 2044 2012 Size: 32 5744 AlwNI CAG_nnn'CTG Cuts at: 2391 3519 5091 2391 Size: 1128 1572 3076 Fragments arranged by size: 3076 1572 1128 ApaLI G'TGCA_C Cuts at: 2300 3833 4989 2300 Size: 1533 1156 3087 Fragments arranged by size: 3087 1533 1156 ApoI r'AATT_y Cuts at: 1493 3085 3311 3463 5572 1493 Size: 1592 226 152 2109 1697 Fragments arranged by size: 2109 1697 1592 226 152 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 3101 1870 Size: 73 1158 4545 Fragments arranged by size: 4545 1158 73 BamHI G'GATC_C Cuts at: 2898 3106 3473 2898 Size: 208 367 5201 Fragments arranged by size: 5201 367 208 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2316 2624 3097 4552 618 Size: 325 217 627 529 308 473 1455 1842 Fragments arranged by size: 1842 1455 627 529 473 325 308 217 BanII G_rGCy'C Cuts at: 1391 2702 2922 3095 1391 Size: 1311 220 173 4072 Fragments arranged by size: 4072 1311 220 173 BbeI G_GCGC'C Cuts at: 2320 2628 2320 Size: 308 5468 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2110 2714 928 Size: 971 211 604 3990 Fragments arranged by size: 3990 971 604 211 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2315 2349 3967 4001 1903 Size: 34 378 34 1618 34 3678 Fragments arranged by size: 3678 1618 378 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4387 136 Size: 107 122 71 631 605 2715 1525 Fragments arranged by size: 2715 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2670 5771 2670 Size: 3101 2675 BlpI GC'TnA_GC Cuts at: 2291 3193 2291 Size: 902 4874 Bme1580I G_kGCm'C Cuts at: 1790 2229 2304 2506 3837 4993 1790 Size: 439 75 202 1331 1156 2573 Fragments arranged by size: 2573 1331 1156 439 202 75 BmgBI CAC'GTC Cuts at: 1318 2368 1318 Size: 1050 4726 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2575 2575 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2686 4436 1239 Size: 1447 1750 2579 Fragments arranged by size: 2579 1750 1447 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3901 4766 5064 3901 Size: 865 298 4613 Fragments arranged by size: 4613 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2054 4439 882 Size: 617 555 2385 2219 Fragments arranged by size: 2385 2219 617 555 BsaAI yAC'GTr Cuts at: 493 1204 2826 3567 493 Size: 711 1622 741 2702 Fragments arranged by size: 2702 1622 741 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2317 2625 3583 3965 Size: 53 83 186 936 784 308 958 382 Cuts at: 3965 4600 275 Size: 635 1451 Fragments arranged by size: 1451 958 936 784 635 382 308 186 83 53 BsaWI w'CCGG_w Cuts at: 4209 4607 4881 5028 5439 4209 Size: 398 274 147 411 4546 Fragments arranged by size: 4546 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2012 2042 2978 3008 1178 Size: 30 165 30 609 30 936 30 3946 Fragments arranged by size: 3946 936 609 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2588 1186 Size: 1402 4374 BseYI C'CCAG_C Cuts at: 2096 2143 2544 4979 5475 2096 Size: 47 401 2435 496 2397 Fragments arranged by size: 2435 2397 496 401 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2362 1163 Size: 573 626 4577 Fragments arranged by size: 4577 626 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2304 2655 2892 2922 3837 3922 4993 2304 Size: 351 237 30 915 85 1071 3087 Fragments arranged by size: 3087 1071 915 351 237 85 30 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 BsmBI CGTCTCn'nnnn_ Cuts at: 2260 2479 2546 2725 2260 Size: 219 67 179 5311 Fragments arranged by size: 5311 219 179 67 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2304 2506 2655 2702 2892 2922 Size: 399 439 75 202 149 47 190 30 Cuts at: 2922 3095 3837 3922 4993 1391 Size: 173 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 439 399 202 190 173 149 85 75 47 30 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 2484 3665 5395 2484 Size: 1181 1730 2865 Fragments arranged by size: 2865 1730 1181 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2097 2187 2643 3131 844 Size: 1253 90 456 488 3489 Fragments arranged by size: 3489 1253 488 456 90 BsrBI CCG'CTC Cuts at: 1394 1823 2641 3663 1394 Size: 429 818 1022 3507 Fragments arranged by size: 3507 1022 818 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4271 4445 65 Size: 1183 3023 174 1396 Fragments arranged by size: 3023 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4420 4607 1700 Size: 110 2610 187 2869 Fragments arranged by size: 2869 2610 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 2303 3836 4848 2303 Size: 1533 1012 3231 Fragments arranged by size: 3231 1533 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5776 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstXI CCAn_nnnn'nTGG Cuts at: 2413 2413 Size: 5776 BstYI r'GATC_y Cuts at: 1972 2670 2898 3106 3473 3858 3875 5327 5413 Size: 698 228 208 367 385 17 1452 86 Cuts at: 5413 5425 5771 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 698 385 367 346 228 208 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2247 513 Size: 552 939 243 4042 Fragments arranged by size: 4042 939 552 243 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 3292 4086 4106 3292 Size: 794 20 4962 Fragments arranged by size: 4962 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DraIII CAC_nnn'GTG Cuts at: 2224 2224 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2271 2382 Size: 54 1425 50 275 18 420 21 111 Cuts at: 2382 2403 3223 4112 5741 8 Size: 21 820 889 1629 43 Fragments arranged by size: 1629 1425 889 820 420 275 111 54 50 43 21 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2660 3706 1353 Size: 1307 1046 3423 Fragments arranged by size: 3423 1307 1046 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2777 4363 4737 4883 387 Size: 1064 138 1188 1586 374 146 1280 Fragments arranged by size: 1586 1280 1188 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2920 2920 Size: 5776 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2686 3839 4436 5223 5491 1239 Size: 393 1054 1153 597 787 268 1524 Fragments arranged by size: 1524 1153 1054 787 597 393 268 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3076 3076 Size: 5776 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 2320 2628 3048 4923 1678 Size: 642 308 420 1875 2531 Fragments arranged by size: 2531 1875 642 420 308 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4417 4449 4491 4523 1373 Size: 32 135 32 2845 32 42 32 2626 Fragments arranged by size: 2845 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3120 3366 669 Size: 988 281 1182 246 3079 Fragments arranged by size: 3079 1182 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KasI G'GCGC_C Cuts at: 2316 2624 2316 Size: 308 5468 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2315 2502 3015 4890 5074 1305 Size: 1010 187 513 1875 184 2007 Fragments arranged by size: 2007 1875 1010 513 187 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3954 Fragments arranged by size: 3954 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2879 3734 4093 4252 518 Size: 648 1713 855 359 159 2042 Fragments arranged by size: 2042 1713 855 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2290 2322 2513 2533 2848 3192 3869 5017 Size: 684 32 191 20 315 344 677 1148 Cuts at: 5017 5262 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 684 677 344 315 245 191 32 20 NarI GG'CG_CC Cuts at: 2317 2625 2317 Size: 308 5468 NcoI C'CATG_G Cuts at: 513 1065 2004 2247 513 Size: 552 939 243 4042 Fragments arranged by size: 4042 939 552 243 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 3134 4679 1132 Size: 97 1905 1545 2229 Fragments arranged by size: 2229 1905 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 2236 2391 1108 Size: 1128 155 4493 Fragments arranged by size: 4493 1128 155 PfoI T'CCnGG_A Cuts at: 2702 2702 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PmlI CAC'GTG Cuts at: 2826 2826 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2012 2044 3845 3877 5358 5390 2012 Size: 32 1801 32 1481 32 2398 Fragments arranged by size: 2398 1801 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PstI C_TGCA'G Cuts at: 830 3128 830 Size: 2298 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 RsrII CG'GwC_CG Cuts at: 2785 2785 Size: 5776 SacI G_AGCT'C Cuts at: 2922 2922 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SapI GCTCTTCn'nnn_ Cuts at: 2660 2660 Size: 5776 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SexAI A'CCwGG_T Cuts at: 2235 2235 Size: 5776 SfcI C'TryA_G Cuts at: 826 1045 3124 4259 4940 5131 826 Size: 219 2079 1135 681 191 1471 Fragments arranged by size: 2079 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2318 2626 2318 Size: 308 5468 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3880 4781 5043 820 Size: 197 926 1937 901 262 1553 Fragments arranged by size: 1937 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5776 StyI C'CwwG_G Cuts at: 513 1065 2004 2247 3215 513 Size: 552 939 243 968 3074 Fragments arranged by size: 3074 968 939 552 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2104 2740 3803 3820 3973 4158 4603 Size: 1255 524 636 1063 17 153 185 445 Cuts at: 4603 325 Size: 1498 Fragments arranged by size: 1498 1255 1063 636 524 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2661 4022 5734 Size: 274 80 33 51 1443 684 1361 1712 Cuts at: 5734 96 Size: 138 Fragments arranged by size: 1712 1443 1361 684 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2294 2326 5736 5768 2294 Size: 32 3410 32 2302 Fragments arranged by size: 3410 2302 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2692 2692 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII SacII SanDI SfiI SrfI SwaI