Restriction map for pFN21AB8999
 (Circular) (Six-base) MAPSORT of: pFN21AB8999.seq  Check: 1334  from: 1  to: 5776



pFN21AB8999  5776 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:28  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3586     278 
   Size:         53      83     186     936    2050    2468

  Fragments arranged by size: 

                2468    2050     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3119    1937 
   Size:       1182    4594
 
Acc65I G'GTAC_C

Cuts at:   3097    3097 
   Size:       5776
 
AclI AA'CG_TT

Cuts at:   1563    3903    4276    1563 
   Size:       2340     373    3063

  Fragments arranged by size: 

                3063    2340     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3839    5223    5491    1632 
   Size:       2207    1384     268    1917

  Fragments arranged by size: 

                2207    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5579
 
AflIII A'CryG_T

Cuts at:   1203    2823    3073    4675    5652    1203 
   Size:       1620     250    1602     977    1327

  Fragments arranged by size: 

                1620    1602    1327     977     250
 
AgeI A'CCGG_T

Cuts at:   4607    4607 
   Size:       5776
 
AhdI GACnn_n'nnGTC

Cuts at:   4505    4505 
   Size:       5776
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5776
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5776
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2012    2044    2012 
   Size:         32    5744
 
AlwNI CAG_nnn'CTG

Cuts at:   2391    3519    5091    2391 
   Size:       1128    1572    3076

  Fragments arranged by size: 

                3076    1572    1128
 
ApaLI G'TGCA_C

Cuts at:   2300    3833    4989    2300 
   Size:       1533    1156    3087

  Fragments arranged by size: 

                3087    1533    1156
 
ApoI r'AATT_y

Cuts at:   1493    3085    3311    3463    5572    1493 
   Size:       1592     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1592     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4330     160 
   Size:        872    3298    1606

  Fragments arranged by size: 

                3298    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5776
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3101    1870 
   Size:         73    1158    4545

  Fragments arranged by size: 

                4545    1158      73
 
BamHI G'GATC_C

Cuts at:   2898    3106    3473    2898 
   Size:        208     367    5201

  Fragments arranged by size: 

                5201     367     208
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2316    2624    3097    4552     618 
   Size:        325     217     627     529     308     473    1455    1842

  Fragments arranged by size: 

                1842    1455     627     529     473     325     308     217
            
 
BanII G_rGCy'C

Cuts at:   1391    2702    2922    3095    1391 
   Size:       1311     220     173    4072

  Fragments arranged by size: 

                4072    1311     220     173
 
BbeI G_GCGC'C

Cuts at:   2320    2628    2320 
   Size:        308    5468
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2110    2714     928 
   Size:        971     211     604    3990

  Fragments arranged by size: 

                3990     971     604     211
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2315    2349    3967    4001    1903 
   Size:         34     378      34    1618      34    3678

  Fragments arranged by size: 

                3678    1618     378      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3668    4878    3668 
   Size:       1210    4566
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5776
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4387     136 
   Size:        107     122      71     631     605    2715    1525

  Fragments arranged by size: 

                2715    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2670    5771    2670 
   Size:       3101    2675
 
BlpI GC'TnA_GC

Cuts at:   2291    3193    2291 
   Size:        902    4874
 
Bme1580I G_kGCm'C

Cuts at:   1790    2229    2304    2506    3837    4993    1790 
   Size:        439      75     202    1331    1156    2573

  Fragments arranged by size: 

                2573    1331    1156     439     202      75
 
BmgBI CAC'GTC

Cuts at:   1318    2368    1318 
   Size:       1050    4726
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3507    4465     442 
   Size:        416      53      83     399      24    2090     958    1753

  Fragments arranged by size: 

                2090    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5776
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2575    2575 
   Size:       5776
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2686    4436    1239 
   Size:       1447    1750    2579

  Fragments arranged by size: 

                2579    1750    1447
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3901    4766    5064    3901 
   Size:        865     298    4613

  Fragments arranged by size: 

                4613     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2054    4439     882 
   Size:        617     555    2385    2219

  Fragments arranged by size: 

                2385    2219     617     555
 
BsaAI yAC'GTr

Cuts at:    493    1204    2826    3567     493 
   Size:        711    1622     741    2702

  Fragments arranged by size: 

                2702    1622     741     711
 
BsaBI GATnn'nnATC

Cuts at:   5770    5770 
   Size:       5776
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2317    2625    3583    3965 
   Size:         53      83     186     936     784     308     958     382

Cuts at:   3965    4600     275 
   Size:        635    1451

  Fragments arranged by size: 

                1451     958     936     784     635     382     308     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   4209    4607    4881    5028    5439    4209 
   Size:        398     274     147     411    4546

  Fragments arranged by size: 

                4546     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2012    2042    2978    3008    1178 
   Size:         30     165      30     609      30     936      30    3946

  Fragments arranged by size: 

                3946     936     609     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2588    1186 
   Size:       1402    4374
 
BseYI C'CCAG_C

Cuts at:   2096    2143    2544    4979    5475    2096 
   Size:         47     401    2435     496    2397

  Fragments arranged by size: 

                2435    2397     496     401      47
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2362    1163 
   Size:        573     626    4577

  Fragments arranged by size: 

                4577     626     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3226    3987    5015    1540 
   Size:        275     186    1225     761    1028    2301

  Fragments arranged by size: 

                2301    1225    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2304    2655    2892    2922    3837    3922    4993    2304 
   Size:        351     237      30     915      85    1071    3087

  Fragments arranged by size: 

                3087    1071     915     351     237      85      30
 
BsmI GAATG_Cn'

Cuts at:   3287    3380    3287 
   Size:         93    5683
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2260    2479    2546    2725    2260 
   Size:        219      67     179    5311

  Fragments arranged by size: 

                5311     219     179      67
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2229    2304    2506    2655    2702    2892    2922 
   Size:        399     439      75     202     149      47     190      30

Cuts at:   2922    3095    3837    3922    4993    1391 
   Size:        173     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     439     399     202     190     173
                 149      85      75      47      30
 
BspEI T'CCGG_A

Cuts at:   5439    5439 
   Size:       5776
 
BspHI T'CATG_A

Cuts at:   2484    3665    5395    2484 
   Size:       1181    1730    2865

  Fragments arranged by size: 

                2865    1730    1181
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2097    2187    2643    3131     844 
   Size:       1253      90     456     488    3489

  Fragments arranged by size: 

                3489    1253     488     456      90
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2641    3663    1394 
   Size:        429     818    1022    3507

  Fragments arranged by size: 

                3507    1022     818     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4271    4445      65 
   Size:       1183    3023     174    1396

  Fragments arranged by size: 

                3023    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4420    4607    1700 
   Size:        110    2610     187    2869

  Fragments arranged by size: 

                2869    2610     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5776
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5776
 
BssSI C'ACGA_G

Cuts at:   2303    3836    4848    2303 
   Size:       1533    1012    3231

  Fragments arranged by size: 

                3231    1533    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5776
 
BstBI TT'CG_AA

Cuts at:   4580    4580 
   Size:       5776
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5776
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2413    2413 
   Size:       5776
 
BstYI r'GATC_y

Cuts at:   1972    2670    2898    3106    3473    3858    3875    5327    5413 
   Size:        698     228     208     367     385      17    1452      86

Cuts at:   5413    5425    5771    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     698     385     367     346     228     208
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2247     513 
   Size:        552     939     243    4042

  Fragments arranged by size: 

                4042     939     552     243
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5037

  Fragments arranged by size: 

                5037     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3292    4086    4106    3292 
   Size:        794      20    4962

  Fragments arranged by size: 

                4962     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5776
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5741
 
DraI TTT'AAA

Cuts at:   3080    3080 
   Size:       5776
 
DraIII CAC_nnn'GTG

Cuts at:   2224    2224 
   Size:       5776
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4783     809 
   Size:        507    3467    1802

  Fragments arranged by size: 

                3467    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2250    2271    2382 
   Size:         54    1425      50     275      18     420      21     111

Cuts at:   2382    2403    3223    4112    5741       8 
   Size:         21     820     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     820     420     275     111      54
                  50      43      21      21      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3223    1537 
   Size:        275    1411    4090

  Fragments arranged by size: 

                4090    1411     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2660    3706    1353 
   Size:       1307    1046    3423

  Fragments arranged by size: 

                3423    1307    1046
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2777    4363    4737    4883     387 
   Size:       1064     138    1188    1586     374     146    1280

  Fragments arranged by size: 

                1586    1280    1188    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2920    2920 
   Size:       5776
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2686    3839    4436    5223    5491    1239 
   Size:        393    1054    1153     597     787     268    1524

  Fragments arranged by size: 

                1524    1153    1054     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   3085    3463    3085 
   Size:        378    5398
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3076    3076 
   Size:       5776
 
FspI TGC'GCA

Cuts at:   4282    4282 
   Size:       5776
 
HaeII r_GCGC'y

Cuts at:   1678    2320    2628    3048    4923    1678 
   Size:        642     308     420    1875    2531

  Fragments arranged by size: 

                2531    1875     642     420     308
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4417    4449    4491    4523    1373 
   Size:         32     135      32    2845      32      42      32    2626

  Fragments arranged by size: 

                2845    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3120    3366     669 
   Size:        988     281    1182     246    3079

  Fragments arranged by size: 

                3079    1182     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5776
 
HpaI GTT'AAC

Cuts at:   3366    3366 
   Size:       5776
 
KasI G'GCGC_C

Cuts at:   2316    2624    2316 
   Size:        308    5468
 
KpnI G_GTAC'C

Cuts at:   3101    3101 
   Size:       5776
 
MfeI C'AATT_G

Cuts at:   3375    3375 
   Size:       5776
 
MluI A'CGCG_T

Cuts at:   5652    5652 
   Size:       5776
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2315    2502    3015    4890    5074    1305 
   Size:       1010     187     513    1875     184    2007

  Fragments arranged by size: 

                2007    1875    1010     513     187     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3954

  Fragments arranged by size: 

                3954    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2879    3734    4093    4252     518 
   Size:        648    1713     855     359     159    2042

  Fragments arranged by size: 

                2042    1713     855     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2290    2322    2513    2533    2848    3192    3869    5017 
   Size:        684      32     191      20     315     344     677    1148

Cuts at:   5017    5262    1606 
   Size:        245    2120

  Fragments arranged by size: 

                2120    1148     684     677     344     315     245     191
                  32      20
 
NarI GG'CG_CC

Cuts at:   2317    2625    2317 
   Size:        308    5468
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2247     513 
   Size:        552     939     243    4042

  Fragments arranged by size: 

                4042     939     552     243
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5776
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5776
 
NotI GC'GGCC_GC

Cuts at:   3223    3223 
   Size:       5776
 
NspI r_CATG'y

Cuts at:   1132    1229    3134    4679    1132 
   Size:         97    1905    1545    2229

  Fragments arranged by size: 

                2229    1905    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5776
 
PciI A'CATG_T

Cuts at:   4675    4675 
   Size:       5776
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2236    2391    1108 
   Size:       1128     155    4493

  Fragments arranged by size: 

                4493    1128     155
 
PfoI T'CCnGG_A

Cuts at:   2702    2702 
   Size:       5776
 
PmeI GTTT'AAAC

Cuts at:   3080    3080 
   Size:       5776
 
PmlI CAC'GTG

Cuts at:   2826    2826 
   Size:       5776
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2012    2044    3845    3877    5358    5390    2012 
   Size:         32    1801      32    1481      32    2398

  Fragments arranged by size: 

                2398    1801    1481      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5776
 
PsiI TTA'TAA

Cuts at:   3346    3346 
   Size:       5776
 
PstI C_TGCA'G

Cuts at:    830    3128     830 
   Size:       2298    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5776
 
RsrII CG'GwC_CG

Cuts at:   2785    2785 
   Size:       5776
 
SacI G_AGCT'C

Cuts at:   2922    2922 
   Size:       5776
 
SalI G'TCGA_C

Cuts at:   1936    3118    1936 
   Size:       1182    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2660    2660 
   Size:       5776
 
SbfI CC_TGCA'GG

Cuts at:   3128    3128 
   Size:       5776
 
ScaI AGT'ACT

Cuts at:   4024    5736    4024 
   Size:       1712    4064
 
SexAI A'CCwGG_T

Cuts at:   2235    2235 
   Size:       5776
 
SfcI C'TryA_G

Cuts at:    826    1045    3124    4259    4940    5131     826 
   Size:        219    2079    1135     681     191    1471

  Fragments arranged by size: 

                2079    1471    1135     681     219     191
 
SfoI GGC'GCC

Cuts at:   2318    2626    2318 
   Size:        308    5468
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5776
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5776
 
SmaI CCC'GGG

Cuts at:   1872    3103    1872 
   Size:       1231    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3880    4781    5043     820 
   Size:        197     926    1937     901     262    1553

  Fragments arranged by size: 

                1937    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3567     493 
   Size:       3074    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5776
 
SphI G_CATG'C

Cuts at:   1132    3134    1132 
   Size:       2002    3774
 
SspI AAT'ATT

Cuts at:      5      52    3700       5 
   Size:         47    3648    2081

  Fragments arranged by size: 

                3648    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5776
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2247    3215     513 
   Size:        552     939     243     968    3074

  Fragments arranged by size: 

                3074     968     939     552     243
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2104    2740    3803    3820    3973    4158    4603 
   Size:       1255     524     636    1063      17     153     185     445

Cuts at:   4603     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255    1063     636     524     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2661    4022    5734 
   Size:        274      80      33      51    1443     684    1361    1712

Cuts at:   5734      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1361     684     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2294    2326    5736    5768    2294 
   Size:         32    3410      32    2302

  Fragments arranged by size: 

                3410    2302      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5776
 
XbaI T'CTAG_A

Cuts at:   3112    3112 
   Size:       5776
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2692    2692 
   Size:       5776
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5776
 
XmaI C'CCGG_G

Cuts at:   1870    3101    1870 
   Size:       1231    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3905    3905 
   Size:       5776
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3584     276 
   Size:         53      83     186     936    2050    2468

  Fragments arranged by size: 

                2468    2050     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NarI     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI     PstI     PvuI    RsrII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI    BbvCI    BfrBI
   Bpu10I    BsiWI  BstZ17I   Bsu36I    EcoNI EcoO109I    EcoRV     FseI
    FspAI     NaeI   NgoMIV     NruI     NsiI     PacI    PpuMI   PspOMI
    PspXI     PsrI    PvuII    SacII    SanDI     SfiI     SrfI     SwaI
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