Restriction map for
pFN21AB8997
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbsI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeII MamI
MfeI MluI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 237 TCCGGA T^CCGGA
AlwNI 1072 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 154 GAGCTC G[AT]GC[AT]^C
AvaI 674 CCCGGG C^[CT]CG[GA]G
BalI 825 TGGCCA TGG^CCA
BbeI 926 GGCGCC GGCGC^C
BbvCI 429 CCTCAGC CCTCAGC(-5/-2)
BcgI 176 CGACACTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 376 CTGGAG CTGGAG(16/14)
BsaWI 237 TCCGGA [AT]^CCGG[AT]
BseAI 237 TCCGGA T^CCGGA
BsgI 1069 GTGCAG GTGCAG(16/14)
BsiEI 766 CGATCG CG[GA][CT]^CG
BsiHKAI 154 GAGCTC G[AT]GC[AT]^C
BsmBI 85 GAGACG CGTCTC(1/5)
BsmI 17 GAATGC GAATGC(1/-1)
BsoBI 674 CCCGGG C^[CT]CG[GA]G
BspEI 237 TCCGGA T^CCGGA
BspMI 489 GCAGGT ACCTGC(4/8)
BsrBI 135 CCGCTC CCGCTC(-3/-3)
BssSI 603 CACGAG CACGAG(-5/-1)
BstXI 297 CCACAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 619 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1011 CCATGG C^C[GA][CT]GG
EcoT14I 1011 CCATGG C^C[AT][AT]GG
KasI 926 GGCGCC G^GCGCC
MluNI 825 TGGCCA TGG^CCA
MroI 237 TCCGGA T^CCGGA
MscI 825 TGGCCA TGG^CCA
MseI 425 TTAA T^TAA
MspA1I 895 CAGCGG C[AC]G^C[GT]G
NaeI 923 GCCGGC GCC^GGC
NarI 926 GGCGCC GG^CGCC
NcoI 1011 CCATGG C^CATGG
NgoMIV 923 GCCGGC G^CCGGC
NheI 699 GCTAGC G^CTAGC
PflMI 688 CCAAACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1074 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 766 CGATCG CGAT^CG
SacI 154 GAGCTC GAGCT^C
SfoI 926 GGCGCC GGC^GCC
SmaI 674 CCCGGG CCC^GGG
StuI 998 AGGCCT AGG^CCT
StyI 1011 CCATGG C^C[AT][AT]GG
TaqI 170 TCGA T^CGA
Tru9I 425 TTAA T^TAA
TthHB8I 170 TCGA T^CGA
Van91I 688 CCAAACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1145 CCATGCTGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 674 CCCGGG C^CCGGG
XmaI 674 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 60 CGCG CG^CG
123 CGCG
AfaI 1152 GTAC GT^AC
1177 GTAC
AlwI 294 GATCC GGATC(4/5)
252 GGATC
ApaI 501 GGGCCC GGGCC^C
794 GGGCCC
BciVI 315 GTATCC GTATCC(6/5)
1100 GTATCC
BglI 331 GCCAGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
827 GCCATGATGGC
BsmAI 85 GAGAC GTCTC(1/5)
606 GAGAC
BsrFI 260 ACCGGC [GA]^CCGG[CT]
923 GCCGGC
BstEII 10 GGTAACC G^GT[ACGT]ACC
816 GGTCACC
BstPI 10 GGTAACC G^GT[ACGT]ACC
816 GGTCACC
BstUI 60 CGCG CG^CG
123 CGCG
BstYI 252 GGATCT [GA]^GATC[CT]
293 AGATCC
Cfr10I 260 ACCGGC [GA]^CCGG[CT]
923 GCCGGC
DraII 500 AGGGCCC [GA]G^G[ACGT]CC[CT]
501 GGGCCCT
EarI 478 CTCTTC CTCTTC(1/4)
286 GAAGAG
EcoO109I 500 AGGGCCC [GA]G^G[ACGT]CC[CT]
501 GGGCCCT
EcoO65I 10 GGTAACC G^GT[ACGT]ACC
816 GGTCACC
HaeII 323 AGCGCC [GA]GCGC^[CT]
926 GGCGCC
HgaI 131 GACGC GACGC(5/10)
857 GACGC
Ksp632I 478 CTCTTC CTCTTC(1/4)
286 GAAGAG
MflI 252 GGATCT [GA]^GATC[CT]
293 AGATCC
MvnI 60 CGCG CG^CG
123 CGCG
NspI 245 ACATGC [GA]CATG^[CT]
750 GCATGT
PleI 714 GACTC GAGTC(4/5)
729 GACTC
PstI 184 CTGCAG CTGCA^G
464 CTGCAG
RsaI 1152 GTAC GT^AC
1177 GTAC
SapI 286 GAAGAGC GCTCTTC(1/4)
477 GCTCTTC
SfaNI 437 GCATC GCATC(5/9)
885 GCATC
SfcI 184 CTGCAG C^T[GA][CT]AG
464 CTGCAG
SfiI 330 GGCCAGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
826 GGCCATGATGGCC
SmlI 310 CTTGAG C^T[CT][GA]AG
776 CTCAAG
TfiI 3 GATTC G^A[AT]TC
111 GATTC
XhoII 252 GGATCT [GA]^GATC[CT]
293 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcyI AluI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BsaHI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TseI Tsp45I Tsp509I TspRI