(Circular) (Six-base) MAPSORT of: pFN21AB8993.seq Check: 1312 from: 1 to: 5908 pFN21AB8993 5908 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3718 278 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3251 1937 Size: 1314 4594 Acc65I G'GTAC_C Cuts at: 3229 3229 Size: 5908 AclI AA'CG_TT Cuts at: 1563 3207 4035 4408 1563 Size: 1644 828 373 3063 Fragments arranged by size: 3063 1644 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2004 2046 2718 3971 5355 5623 1632 Size: 372 42 672 1253 1384 268 1917 Fragments arranged by size: 1917 1384 1253 672 372 268 42 AflII C'TTAA_G Cuts at: 820 1017 2509 820 Size: 197 1492 4219 Fragments arranged by size: 4219 1492 197 AflIII A'CryG_T Cuts at: 1203 2011 4807 5784 1203 Size: 808 2796 977 1327 Fragments arranged by size: 2796 1327 977 808 AgeI A'CCGG_T Cuts at: 4739 4739 Size: 5908 AhdI GACnn_n'nnGTC Cuts at: 2122 4637 2122 Size: 2515 3393 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5908 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5908 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 2085 Size: 32 5876 AlwNI CAG_nnn'CTG Cuts at: 3651 5223 3651 Size: 1572 4336 ApaLI G'TGCA_C Cuts at: 3965 5121 3965 Size: 1156 4752 ApoI r'AATT_y Cuts at: 1493 2349 2439 2449 2539 2734 2785 2950 3217 Size: 856 90 10 90 195 51 165 267 Cuts at: 3217 3443 3595 5704 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 856 267 226 195 165 152 90 90 51 10 AseI AT'TA_AT Cuts at: 160 1032 2325 4462 160 Size: 872 1293 2137 1606 Fragments arranged by size: 2137 1606 1293 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 AvaI C'yCGr_G Cuts at: 1870 1943 3233 1870 Size: 73 1290 4545 Fragments arranged by size: 4545 1290 73 BamHI G'GATC_C Cuts at: 2165 3238 3605 2165 Size: 1073 367 4468 Fragments arranged by size: 4468 1073 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3229 4684 618 Size: 325 217 627 1442 1455 1842 Fragments arranged by size: 1842 1455 1442 627 325 217 BanII G_rGCy'C Cuts at: 1391 3227 1391 Size: 1836 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4937 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4099 4133 1903 Size: 34 2162 34 3678 Fragments arranged by size: 3678 2162 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3800 5010 3800 Size: 1210 4698 BclI T'GATC_A Cuts at: 1548 1548 Size: 5908 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4519 136 Size: 107 122 71 631 605 2847 1525 Fragments arranged by size: 2847 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2459 5903 2459 Size: 3444 2464 BlpI GC'TnA_GC Cuts at: 3325 3325 Size: 5908 Bme1580I G_kGCm'C Cuts at: 1790 3969 5125 1790 Size: 2179 1156 2573 Fragments arranged by size: 2573 2179 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5908 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3639 4597 442 Size: 416 53 83 399 24 2222 958 1753 Fragments arranged by size: 2222 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3164 1056 Size: 2108 3800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4568 1239 Size: 3329 2579 Bpu10I CC'TnA_GC Cuts at: 2689 2689 Size: 5908 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2013 4033 4898 5196 2013 Size: 2020 865 298 2725 Fragments arranged by size: 2725 2020 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2085 4571 882 Size: 617 586 2486 2219 Fragments arranged by size: 2486 2219 617 586 BsaAI yAC'GTr Cuts at: 493 1204 3699 493 Size: 711 2495 2702 Fragments arranged by size: 2702 2495 711 BsaBI GATnn'nnATC Cuts at: 5902 5902 Size: 5908 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3715 4097 4732 275 Size: 53 83 186 936 2182 382 635 1451 Fragments arranged by size: 2182 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4341 4739 5013 5160 5571 4341 Size: 398 274 147 411 4678 Fragments arranged by size: 4678 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2065 2095 2350 2380 1178 Size: 30 165 30 662 30 255 30 4706 Fragments arranged by size: 4706 662 255 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2808 1186 Size: 1622 4286 BseYI C'CCAG_C Cuts at: 5111 5607 5111 Size: 496 5412 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5335 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3129 3358 4119 5147 1540 Size: 275 186 1128 229 761 1028 2301 Fragments arranged by size: 2301 1128 1028 761 275 229 186 BsiHKAI G_wGCw'C Cuts at: 3969 4054 5125 3969 Size: 85 1071 4752 Fragments arranged by size: 4752 1071 85 BsmI GAATG_Cn' Cuts at: 3419 3512 3419 Size: 93 5815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3227 3969 4054 5125 1391 Size: 399 1437 742 85 1071 2174 Fragments arranged by size: 2174 1437 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5571 5571 Size: 5908 BspHI T'CATG_A Cuts at: 3797 5527 3797 Size: 1730 4178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3263 844 Size: 2419 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3795 1394 Size: 429 1972 3507 Fragments arranged by size: 3507 1972 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4403 4577 65 Size: 1183 3155 174 1396 Fragments arranged by size: 3155 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4552 4739 1700 Size: 110 2742 187 2869 Fragments arranged by size: 2869 2742 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5908 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5908 BssSI C'ACGA_G Cuts at: 3968 4980 3968 Size: 1012 4896 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5908 BstBI TT'CG_AA Cuts at: 4712 4712 Size: 5908 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5908 BstYI r'GATC_y Cuts at: 1972 2165 2459 3238 3605 3990 4007 5459 5545 Size: 193 294 779 367 385 17 1452 86 Cuts at: 5545 5557 5903 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 779 385 367 346 294 193 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3107 513 Size: 552 939 1103 3314 Fragments arranged by size: 3314 1103 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5169 Fragments arranged by size: 5169 683 56 BtsI GCAGTG_nn' Cuts at: 3424 4218 4238 3424 Size: 794 20 5094 Fragments arranged by size: 5094 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5908 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5873 DraI TTT'AAA Cuts at: 2739 2883 3212 2739 Size: 144 329 5435 Fragments arranged by size: 5435 329 144 DraIII CAC_nnn'GTG Cuts at: 2947 2947 Size: 5908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4915 809 Size: 507 3599 1802 Fragments arranged by size: 3599 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3355 4244 5873 Size: 54 1425 50 275 18 1525 889 1629 Cuts at: 5873 8 Size: 43 Fragments arranged by size: 1629 1525 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3355 1537 Size: 275 1543 4090 Fragments arranged by size: 4090 1543 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2342 2484 2573 3171 3838 1353 Size: 989 142 89 598 667 3423 Fragments arranged by size: 3423 989 667 598 142 89 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4495 4869 5015 387 Size: 1064 138 2906 374 146 1280 Fragments arranged by size: 2906 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2004 2046 2718 3971 4568 5355 5623 Size: 393 372 42 672 1253 597 787 268 Cuts at: 5623 1239 Size: 1524 Fragments arranged by size: 1524 1253 787 672 597 393 372 268 42 EcoNI CCTnn'n_nnAGG Cuts at: 2074 2074 Size: 5908 EcoRI G'AATT_C Cuts at: 3217 3595 3217 Size: 378 5530 EcoRV GAT'ATC Cuts at: 3050 3050 Size: 5908 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2778 2778 Size: 5908 FspI TGC'GCA Cuts at: 4414 4414 Size: 5908 HaeII r_GCGC'y Cuts at: 1678 5055 1678 Size: 3377 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2140 2483 2515 4549 Size: 32 135 32 536 32 343 32 2034 Cuts at: 4549 4581 4623 4655 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2034 536 343 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2892 3252 3498 669 Size: 988 281 954 360 246 3079 Fragments arranged by size: 3079 988 954 360 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5908 HpaI GTT'AAC Cuts at: 3498 3498 Size: 5908 KpnI G_GTAC'C Cuts at: 3233 3233 Size: 5908 MfeI C'AATT_G Cuts at: 3507 3507 Size: 5908 MluI A'CGCG_T Cuts at: 5784 5784 Size: 5908 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2821 5022 5206 1305 Size: 1516 2201 184 2007 Fragments arranged by size: 2201 2007 1516 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4086 Fragments arranged by size: 4086 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3866 4225 4384 518 Size: 648 2700 359 159 2042 Fragments arranged by size: 2700 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3143 3324 4001 5149 5394 1606 Size: 1537 181 677 1148 245 2120 Fragments arranged by size: 2120 1537 1148 677 245 181 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4417 Fragments arranged by size: 4417 939 552 NdeI CA'TA_TG Cuts at: 387 2904 387 Size: 2517 3391 NheI G'CTAG_C Cuts at: 1052 3160 1052 Size: 2108 3800 NotI GC'GGCC_GC Cuts at: 3355 3355 Size: 5908 NspI r_CATG'y Cuts at: 1132 1229 2015 2144 2876 2929 3091 3266 4811 Size: 97 786 129 732 53 162 175 1545 Cuts at: 4811 1132 Size: 2229 Fragments arranged by size: 2229 1545 786 732 175 162 129 97 53 PasI CC'CwG_GG Cuts at: 1348 2105 1348 Size: 757 5151 PciI A'CATG_T Cuts at: 2011 4807 2011 Size: 2796 3112 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5908 PmeI GTTT'AAAC Cuts at: 3212 3212 Size: 5908 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3977 4009 5490 5522 3977 Size: 32 1481 32 4363 Fragments arranged by size: 4363 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5908 PsiI TTA'TAA Cuts at: 2532 3478 2532 Size: 946 4962 PstI C_TGCA'G Cuts at: 830 3260 830 Size: 2430 3478 PvuI CG_AT'CG Cuts at: 2001 3129 2001 Size: 1128 4780 PvuII CAG'CTG Cuts at: 3143 3143 Size: 5908 SalI G'TCGA_C Cuts at: 1936 3250 1936 Size: 1314 4594 SapI GCTCTTCn'nnn_ Cuts at: 3171 3171 Size: 5908 SbfI CC_TGCA'GG Cuts at: 3260 3260 Size: 5908 ScaI AGT'ACT Cuts at: 4156 5868 4156 Size: 1712 4196 SfcI C'TryA_G Cuts at: 826 1045 3256 4391 5072 5263 826 Size: 219 2211 1135 681 191 1471 Fragments arranged by size: 2211 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5908 SmaI CCC'GGG Cuts at: 1872 3235 1872 Size: 1363 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2028 2509 4012 4913 5175 820 Size: 197 926 85 481 1503 901 262 1553 Fragments arranged by size: 1553 1503 926 901 481 262 197 85 SnaBI TAC'GTA Cuts at: 493 3699 493 Size: 3206 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5908 SphI G_CATG'C Cuts at: 1132 3266 1132 Size: 2134 3774 SspI AAT'ATT Cuts at: 5 52 2379 3832 5 Size: 47 2327 1453 2081 Fragments arranged by size: 2327 2081 1453 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5908 StyI C'CwwG_G Cuts at: 513 1065 2004 3347 513 Size: 552 939 1343 3074 Fragments arranged by size: 3074 1343 939 552 SwaI ATTT'AAAT Cuts at: 2739 2739 Size: 5908 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3935 3952 4105 4290 4735 325 Size: 1255 2355 17 153 185 445 1498 Fragments arranged by size: 2355 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4154 5866 96 Size: 274 80 33 51 1443 2177 1712 138 Fragments arranged by size: 2177 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2065 2097 3034 3066 5868 5900 2065 Size: 32 937 32 2802 32 2073 Fragments arranged by size: 2802 2073 937 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5908 XbaI T'CTAG_A Cuts at: 3244 3244 Size: 5908 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5908 XmaI C'CCGG_G Cuts at: 1870 3233 1870 Size: 1363 4545 XmnI GAAnn'nnTTC Cuts at: 4037 4037 Size: 5908 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3716 276 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI XcmI