Restriction map for
pFN21AB8990
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BcgI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaHI BsaI BsiWI BsoBI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MfeI MluI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tsp509I Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 584 TCCGGA T^CCGGA
Aor51HI 1010 AGCGCT AGC^GCT
ApaI 103 GGGCCC GGGCC^C
Asp700I 1075 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 564 GAGCTC G[AT]GC[AT]^C
BalI 209 TGGCCA TGG^CCA
BbsI 241 GTCTTC GAAGAC(2/6)
BbvCI 234 CCTCAGC CCTCAGC(-5/-2)
BciVI 876 GGATAC GTATCC(6/5)
BglII 278 AGATCT A^GATCT
BpmI 903 CTCCAG CTGGAG(16/14)
BpuAI 241 GTCTTC GAAGAC(2/6)
BsaAI 994 TACGTG [CT]AC^GT[GA]
BsaBI 732 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 584 TCCGGA [AT]^CCGG[AT]
BseAI 584 TCCGGA T^CCGGA
BseRI 795 GAGGAG GAGGAG(10/8)
BsiEI 459 CGACCG CG[GA][CT]^CG
BsiHKAI 564 GAGCTC G[AT]GC[AT]^C
BsmAI 379 GAGAC GTCTC(1/5)
BsmBI 379 GAGACG CGTCTC(1/5)
BsmFI 118 GGGAC GGGAC(10/14)
BsmI 399 GAATGC GAATGC(1/-1)
Bsp1407I 422 TGTACA T^GTACA
BspDI 928 ATCGAT AT^CGAT
BspEI 584 TCCGGA T^CCGGA
BsrGI 422 TGTACA T^GTACA
ClaI 928 ATCGAT AT^CGAT
Eco47III 1010 AGCGCT AGC^GCT
EcoRV 18 GATATC GAT^ATC
FauI 323 GCGGG CCCGC(4/6)
MamI 732 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 209 TGGCCA TGG^CCA
MroI 584 TCCGGA T^CCGGA
MscI 209 TGGCCA TGG^CCA
MseI 113 TTAA T^TAA
PleI 46 GACTC GAGTC(4/5)
PpuMI 118 GGGACCT [GA]G^G[AT]CC[CT]
SacI 564 GAGCTC GAGCT^C
SspBI 422 TGTACA T^GTACA
TfiI 1137 GATTC G^A[AT]TC
Tru9I 113 TTAA T^TAA
XcmI 646 CCATCCCTCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1075 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 391 CGCG CG^CG
505 CGCG
AlwI 312 GATCC GGATC(4/5)
783 GATCC
AlwNI 429 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
745 CAGGTGCTG
BanII 103 GGGCCC G[GA]GC[CT]^C
564 GAGCTC
BfaI 217 CTAG C^TAG
811 CTAG
BspMI 744 GCAGGT ACCTGC(4/8)
1067 GCAGGT
BstUI 391 CGCG CG^CG
505 CGCG
BstYI 278 AGATCT [GA]^GATC[CT]
782 AGATCC
DraII 102 AGGGCCC [GA]G^G[ACGT]CC[CT]
118 GGGACCT
EaeI 209 TGGCCA [CT]^GGCC[GA]
296 TGGCCG
EcoO109I 102 AGGGCCC [GA]G^G[ACGT]CC[CT]
118 GGGACCT
EcoT14I 491 CCATGG C^C[AT][AT]GG
1097 CCATGG
HaeII 73 AGCGCC [GA]GCGC^[CT]
1010 AGCGCT
HapII 498 CCGG C^CGG
585 CCGG
HgaI 381 GACGC GACGC(5/10)
239 GCGTC
HinfI 46 GACTC G^A[ACGT]TC
1137 GATTC
HpaII 498 CCGG C^CGG
585 CCGG
MaeI 217 CTAG C^TAG
811 CTAG
MaeII 620 ACGT A^CGT
995 ACGT
MaeIII 91 GTCAC ^GT[ACGT]AC
622 GTCAC
MboII 15 GAAGA GAAGA(8/7)
242 TCTTC
MflI 278 AGATCT [GA]^GATC[CT]
782 AGATCC
MspI 498 CCGG C^CGG
585 CCGG
MvnI 391 CGCG CG^CG
505 CGCG
NcoI 491 CCATGG C^CATGG
1097 CCATGG
NspI 80 ACATGC [GA]CATG^[CT]
674 ACATGC
PstI 1065 CTGCAG CTGCA^G
1147 CTGCAG
StyI 491 CCATGG C^C[AT][AT]GG
1097 CCATGG
Tsp45I 91 GTCAC ^GT[GC]AC
622 GTCAC
TspRI 986 CACTG CA[GC]TG[ACGT][ACGT]^
1106 CACTG
XhoII 278 AGATCT [GA]^GATC[CT]
782 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BmyI BsaJI BsgI BsiYI BslI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HphI
ItaI MboI MnlI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI TthHB8I