(Circular) (Six-base) MAPSORT of: pFN21AB8990.seq Check: 8214 from: 1 to: 5878 pFN21AB8990 5878 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2740 2740 Size: 5878 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3221 1937 Size: 1284 4594 Acc65I G'GTAC_C Cuts at: 3199 3199 Size: 5878 AclI AA'CG_TT Cuts at: 1563 4005 4378 1563 Size: 2442 373 3063 Fragments arranged by size: 3063 2442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3941 5325 5593 1632 Size: 2309 1384 268 1917 Fragments arranged by size: 2309 1917 1384 268 AfeI AGC'GCT Cuts at: 3017 3017 Size: 5878 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 4777 5754 1203 Size: 3574 977 1327 Fragments arranged by size: 3574 1327 977 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 4607 4607 Size: 5878 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5878 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5878 AlwNI CAG_nnn'CTG Cuts at: 2439 2755 3621 5193 2439 Size: 316 866 1572 3124 Fragments arranged by size: 3124 1572 866 316 ApaI G_GGCC'C Cuts at: 2112 2112 Size: 5878 ApaLI G'TGCA_C Cuts at: 3935 5091 3935 Size: 1156 4722 ApoI r'AATT_y Cuts at: 1493 3187 3413 3565 5674 1493 Size: 1694 226 152 2109 1697 Fragments arranged by size: 2109 1697 1694 226 152 AseI AT'TA_AT Cuts at: 160 1032 4432 160 Size: 872 3400 1606 Fragments arranged by size: 3400 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 3203 1870 Size: 73 1260 4545 Fragments arranged by size: 4545 1260 73 BamHI G'GATC_C Cuts at: 3208 3575 3208 Size: 367 5511 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3199 4654 618 Size: 325 217 627 1412 1455 1842 Fragments arranged by size: 1842 1455 1412 627 325 217 BanII G_rGCy'C Cuts at: 1391 2112 2573 3197 1391 Size: 721 461 624 4072 Fragments arranged by size: 4072 721 624 461 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2239 928 Size: 971 340 4567 Fragments arranged by size: 4567 971 340 BbvCI CC'TCA_GC Cuts at: 2240 2240 Size: 5878 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4069 4103 1903 Size: 34 2132 34 3678 Fragments arranged by size: 3678 2132 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2875 3770 4980 2875 Size: 895 1210 3773 Fragments arranged by size: 3773 1210 895 BclI T'GATC_A Cuts at: 1548 1548 Size: 5878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4489 136 Size: 107 122 71 631 605 2817 1525 Fragments arranged by size: 2817 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2283 5873 2283 Size: 3590 2288 BlpI GC'TnA_GC Cuts at: 3295 3295 Size: 5878 Bme1580I G_kGCm'C Cuts at: 1790 2061 2112 3110 3939 5095 1790 Size: 271 51 998 829 1156 2573 Fragments arranged by size: 2573 1156 998 829 271 51 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5878 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3169 3609 4567 Size: 416 53 83 399 24 1752 440 958 Cuts at: 4567 442 Size: 1753 Fragments arranged by size: 1753 1752 958 440 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2893 4538 1239 Size: 1654 1645 2579 Fragments arranged by size: 2579 1654 1645 Bpu10I CC'TnA_GC Cuts at: 2240 2567 2240 Size: 327 5551 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4003 4868 5166 4003 Size: 865 298 4715 Fragments arranged by size: 4715 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4541 882 Size: 617 3042 2219 Fragments arranged by size: 3042 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3001 3669 493 Size: 711 1797 668 2702 Fragments arranged by size: 2702 1797 711 668 BsaBI GATnn'nnATC Cuts at: 2741 5872 2741 Size: 3131 2747 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3685 4067 4702 275 Size: 53 83 186 936 2152 382 635 1451 Fragments arranged by size: 2152 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2589 4311 4709 4983 5130 5541 2589 Size: 1722 398 274 147 411 2926 Fragments arranged by size: 2926 1722 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5653 Fragments arranged by size: 5653 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2815 1186 Size: 1629 4249 BseYI C'CCAG_C Cuts at: 2161 2520 5081 5577 2161 Size: 359 2561 496 2462 Fragments arranged by size: 2561 2462 496 359 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2240 2254 2716 1163 Size: 573 504 14 462 4325 Fragments arranged by size: 4325 573 504 462 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2467 3328 4089 5117 1540 Size: 275 186 466 861 761 1028 2301 Fragments arranged by size: 2301 1028 861 761 466 275 186 BsiHKAI G_wGCw'C Cuts at: 2573 3939 4024 5095 2573 Size: 1366 85 1071 3356 Fragments arranged by size: 3356 1366 1071 85 BsmI GAATG_Cn' Cuts at: 2410 3389 3482 2410 Size: 979 93 4806 Fragments arranged by size: 4806 979 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2378 2378 Size: 5878 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2112 2573 3110 3197 3939 4024 Size: 399 271 51 461 537 87 742 85 Cuts at: 4024 5095 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 537 461 399 271 87 85 51 BspEI T'CCGG_A Cuts at: 2589 5541 2589 Size: 2952 2926 BspHI T'CATG_A Cuts at: 3767 5497 3767 Size: 1730 4148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2740 3063 3233 844 Size: 1896 323 170 3489 Fragments arranged by size: 3489 1896 323 170 BsrBI CCG'CTC Cuts at: 1394 1823 3765 1394 Size: 429 1942 3507 Fragments arranged by size: 3507 1942 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4373 4547 65 Size: 1183 3125 174 1396 Fragments arranged by size: 3125 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4522 4709 1700 Size: 110 2712 187 2869 Fragments arranged by size: 2869 2712 187 110 BsrGI T'GTAC_A Cuts at: 96 2427 96 Size: 2331 3547 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5878 BssSI C'ACGA_G Cuts at: 3938 4950 3938 Size: 1012 4866 BstAPI GCAn_nnn'nTGC Cuts at: 794 2746 2755 2979 794 Size: 1952 9 224 3693 Fragments arranged by size: 3693 1952 224 9 BstBI TT'CG_AA Cuts at: 4682 4682 Size: 5878 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstXI CCAn_nnnn'nTGG Cuts at: 2497 3091 3130 2497 Size: 594 39 5245 Fragments arranged by size: 5245 594 39 BstYI r'GATC_y Cuts at: 1972 2283 2787 3208 3575 3960 3977 5429 5515 Size: 311 504 421 367 385 17 1452 86 Cuts at: 5515 5527 5873 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 504 421 385 367 346 311 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2064 2496 2808 3102 3129 513 Size: 552 999 432 312 294 27 3262 Fragments arranged by size: 3262 999 552 432 312 294 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2625 2876 487 Size: 683 56 1399 251 3489 Fragments arranged by size: 3489 1399 683 251 56 BtsI GCAGTG_nn' Cuts at: 3110 3394 4188 4208 3110 Size: 284 794 20 4780 Fragments arranged by size: 4780 794 284 20 ClaI AT'CG_AT Cuts at: 1554 2934 1554 Size: 1380 4498 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2138 2173 529 Size: 35 1574 35 4234 Fragments arranged by size: 4234 1574 35 35 DraI TTT'AAA Cuts at: 3182 3182 Size: 5878 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4885 809 Size: 507 3569 1802 Fragments arranged by size: 3569 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2214 2301 3325 Size: 54 1425 50 275 18 384 87 1024 Cuts at: 3325 4214 5843 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1024 889 384 275 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3325 1537 Size: 275 1513 4090 Fragments arranged by size: 4090 1513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3808 1353 Size: 2455 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4465 4839 4985 387 Size: 1064 138 2876 374 146 1280 Fragments arranged by size: 2876 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2571 2571 Size: 5878 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2893 3941 4538 5325 5593 1239 Size: 393 1261 1048 597 787 268 1524 Fragments arranged by size: 1524 1261 1048 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2108 2124 2108 Size: 16 5862 EcoRI G'AATT_C Cuts at: 3187 3565 3187 Size: 378 5500 EcoRV GAT'ATC Cuts at: 2025 2025 Size: 5878 FspI TGC'GCA Cuts at: 4384 4384 Size: 5878 HaeII r_GCGC'y Cuts at: 1678 2082 3019 5025 1678 Size: 404 937 2006 2531 Fragments arranged by size: 2531 2006 937 404 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4519 4551 4593 4625 1373 Size: 32 135 32 2947 32 42 32 2626 Fragments arranged by size: 2947 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3222 3468 669 Size: 988 281 1284 246 3079 Fragments arranged by size: 3079 1284 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5878 HpaI GTT'AAC Cuts at: 3468 3468 Size: 5878 KpnI G_GTAC'C Cuts at: 3203 3203 Size: 5878 MfeI C'AATT_G Cuts at: 3477 3477 Size: 5878 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2382 4992 5176 1305 Size: 1077 2610 184 2007 Fragments arranged by size: 2610 2007 1077 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2216 10 Size: 54 1425 343 384 3672 Fragments arranged by size: 3672 1425 384 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 2582 2678 2750 3089 3128 3836 Size: 648 1329 87 96 72 339 39 708 Cuts at: 3836 4195 4354 518 Size: 359 159 2042 Fragments arranged by size: 2042 1329 708 648 359 339 159 96 87 72 39 MspA1I CmG'CkG Cuts at: 1606 2268 2913 2979 3294 3971 5119 5364 1606 Size: 662 645 66 315 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 662 645 315 245 66 NcoI C'CATG_G Cuts at: 513 1065 2496 3102 513 Size: 552 1431 606 3289 Fragments arranged by size: 3289 1431 606 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5878 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 3325 3325 Size: 5878 NspI r_CATG'y Cuts at: 1132 1229 2089 2683 3236 4781 1132 Size: 97 860 594 553 1545 2229 Fragments arranged by size: 2229 1545 860 594 553 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5878 PciI A'CATG_T Cuts at: 4777 4777 Size: 5878 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5878 PfoI T'CCnGG_A Cuts at: 2877 2877 Size: 5878 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3947 3979 5460 5492 3947 Size: 32 1481 32 4333 Fragments arranged by size: 4333 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2124 2124 Size: 5878 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5878 PsiI TTA'TAA Cuts at: 3448 3448 Size: 5878 PspOMI G'GGCC_C Cuts at: 2108 2108 Size: 5878 PstI C_TGCA'G Cuts at: 830 3074 3156 3230 830 Size: 2244 82 74 3478 Fragments arranged by size: 3478 2244 82 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5878 PvuII CAG'CTG Cuts at: 2268 2913 2979 2268 Size: 645 66 5167 Fragments arranged by size: 5167 645 66 SacI G_AGCT'C Cuts at: 2573 2573 Size: 5878 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SbfI CC_TGCA'GG Cuts at: 3230 3230 Size: 5878 ScaI AGT'ACT Cuts at: 4126 5838 4126 Size: 1712 4166 SfcI C'TryA_G Cuts at: 826 1045 2533 2968 3070 3152 3226 4361 5042 Size: 219 1488 435 102 82 74 1135 681 Cuts at: 5042 5233 826 Size: 191 1471 Fragments arranged by size: 1488 1471 1135 681 435 219 191 102 82 74 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5878 SmaI CCC'GGG Cuts at: 1872 3205 1872 Size: 1333 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3982 4883 5145 820 Size: 197 926 2039 901 262 1553 Fragments arranged by size: 2039 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5878 SphI G_CATG'C Cuts at: 1132 3236 1132 Size: 2104 3774 SspI AAT'ATT Cuts at: 5 52 3802 5 Size: 47 3750 2081 Fragments arranged by size: 3750 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5878 StyI C'CwwG_G Cuts at: 513 1065 2496 3102 3317 513 Size: 552 1431 606 215 3074 Fragments arranged by size: 3074 1431 606 552 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2188 2590 3905 3922 4075 4260 4705 Size: 1255 608 402 1315 17 153 185 445 Cuts at: 4705 325 Size: 1498 Fragments arranged by size: 1498 1315 1255 608 445 402 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2427 2604 4124 Size: 274 80 33 51 1443 450 177 1520 Cuts at: 4124 5836 96 Size: 1712 138 Fragments arranged by size: 1712 1520 1443 450 274 177 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5838 5870 5838 Size: 32 5846 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5878 XbaI T'CTAG_A Cuts at: 3214 3214 Size: 5878 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2658 2658 Size: 5878 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5878 XmaI C'CCGG_G Cuts at: 1870 3203 1870 Size: 1333 4545 XmnI GAAnn'nnTTC Cuts at: 3084 4007 3084 Size: 923 4955 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI