Restriction map for pFN21AB8990
 (Circular) (Six-base) MAPSORT of: pFN21AB8990.seq  Check: 8214  from: 1  to: 5878



pFN21AB8990  5878 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2740    2740 
   Size:       5878
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3688     278 
   Size:         53      83     186     936    2152    2468

  Fragments arranged by size: 

                2468    2152     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3221    1937 
   Size:       1284    4594
 
Acc65I G'GTAC_C

Cuts at:   3199    3199 
   Size:       5878
 
AclI AA'CG_TT

Cuts at:   1563    4005    4378    1563 
   Size:       2442     373    3063

  Fragments arranged by size: 

                3063    2442     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3941    5325    5593    1632 
   Size:       2309    1384     268    1917

  Fragments arranged by size: 

                2309    1917    1384     268
 
AfeI AGC'GCT

Cuts at:   3017    3017 
   Size:       5878
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5681
 
AflIII A'CryG_T

Cuts at:   1203    4777    5754    1203 
   Size:       3574     977    1327

  Fragments arranged by size: 

                3574    1327     977
 
AgeI A'CCGG_T

Cuts at:   4709    4709 
   Size:       5878
 
AhdI GACnn_n'nnGTC

Cuts at:   4607    4607 
   Size:       5878
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5878
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5878
 
AlwNI CAG_nnn'CTG

Cuts at:   2439    2755    3621    5193    2439 
   Size:        316     866    1572    3124

  Fragments arranged by size: 

                3124    1572     866     316
 
ApaI G_GGCC'C

Cuts at:   2112    2112 
   Size:       5878
 
ApaLI G'TGCA_C

Cuts at:   3935    5091    3935 
   Size:       1156    4722
 
ApoI r'AATT_y

Cuts at:   1493    3187    3413    3565    5674    1493 
   Size:       1694     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1694     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4432     160 
   Size:        872    3400    1606

  Fragments arranged by size: 

                3400    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5878
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3203    1870 
   Size:         73    1260    4545

  Fragments arranged by size: 

                4545    1260      73
 
BamHI G'GATC_C

Cuts at:   3208    3575    3208 
   Size:        367    5511
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3199    4654     618 
   Size:        325     217     627    1412    1455    1842

  Fragments arranged by size: 

                1842    1455    1412     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2112    2573    3197    1391 
   Size:        721     461     624    4072

  Fragments arranged by size: 

                4072     721     624     461
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2239     928 
   Size:        971     340    4567

  Fragments arranged by size: 

                4567     971     340
 
BbvCI CC'TCA_GC

Cuts at:   2240    2240 
   Size:       5878
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4069    4103    1903 
   Size:         34    2132      34    3678

  Fragments arranged by size: 

                3678    2132      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2875    3770    4980    2875 
   Size:        895    1210    3773

  Fragments arranged by size: 

                3773    1210     895
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5878
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4489     136 
   Size:        107     122      71     631     605    2817    1525

  Fragments arranged by size: 

                2817    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2283    5873    2283 
   Size:       3590    2288
 
BlpI GC'TnA_GC

Cuts at:   3295    3295 
   Size:       5878
 
Bme1580I G_kGCm'C

Cuts at:   1790    2061    2112    3110    3939    5095    1790 
   Size:        271      51     998     829    1156    2573

  Fragments arranged by size: 

                2573    1156     998     829     271      51
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5878
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3169    3609    4567 
   Size:        416      53      83     399      24    1752     440     958

Cuts at:   4567     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1752     958     440     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5878
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2893    4538    1239 
   Size:       1654    1645    2579

  Fragments arranged by size: 

                2579    1654    1645
 
Bpu10I CC'TnA_GC

Cuts at:   2240    2567    2240 
   Size:        327    5551
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4003    4868    5166    4003 
   Size:        865     298    4715

  Fragments arranged by size: 

                4715     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4541     882 
   Size:        617    3042    2219

  Fragments arranged by size: 

                3042    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3001    3669     493 
   Size:        711    1797     668    2702

  Fragments arranged by size: 

                2702    1797     711     668
 
BsaBI GATnn'nnATC

Cuts at:   2741    5872    2741 
   Size:       3131    2747
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3685    4067    4702     275 
   Size:         53      83     186     936    2152     382     635    1451

  Fragments arranged by size: 

                2152    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2589    4311    4709    4983    5130    5541    2589 
   Size:       1722     398     274     147     411    2926

  Fragments arranged by size: 

                2926    1722     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5653

  Fragments arranged by size: 

                5653     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2815    1186 
   Size:       1629    4249
 
BseYI C'CCAG_C

Cuts at:   2161    2520    5081    5577    2161 
   Size:        359    2561     496    2462

  Fragments arranged by size: 

                2561    2462     496     359
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2240    2254    2716    1163 
   Size:        573     504      14     462    4325

  Fragments arranged by size: 

                4325     573     504     462      14
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2467    3328    4089    5117    1540 
   Size:        275     186     466     861     761    1028    2301

  Fragments arranged by size: 

                2301    1028     861     761     466     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2573    3939    4024    5095    2573 
   Size:       1366      85    1071    3356

  Fragments arranged by size: 

                3356    1366    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2410    3389    3482    2410 
   Size:        979      93    4806

  Fragments arranged by size: 

                4806     979      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2378    2378 
   Size:       5878
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2061    2112    2573    3110    3197    3939    4024 
   Size:        399     271      51     461     537      87     742      85

Cuts at:   4024    5095    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     537     461     399     271      87
                  85      51
 
BspEI T'CCGG_A

Cuts at:   2589    5541    2589 
   Size:       2952    2926
 
BspHI T'CATG_A

Cuts at:   3767    5497    3767 
   Size:       1730    4148
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2740    3063    3233     844 
   Size:       1896     323     170    3489

  Fragments arranged by size: 

                3489    1896     323     170
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3765    1394 
   Size:        429    1942    3507

  Fragments arranged by size: 

                3507    1942     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4373    4547      65 
   Size:       1183    3125     174    1396

  Fragments arranged by size: 

                3125    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4522    4709    1700 
   Size:        110    2712     187    2869

  Fragments arranged by size: 

                2869    2712     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2427      96 
   Size:       2331    3547
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5878
 
BssSI C'ACGA_G

Cuts at:   3938    4950    3938 
   Size:       1012    4866
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2746    2755    2979     794 
   Size:       1952       9     224    3693

  Fragments arranged by size: 

                3693    1952     224       9
 
BstBI TT'CG_AA

Cuts at:   4682    4682 
   Size:       5878
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5878
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2497    3091    3130    2497 
   Size:        594      39    5245

  Fragments arranged by size: 

                5245     594      39
 
BstYI r'GATC_y

Cuts at:   1972    2283    2787    3208    3575    3960    3977    5429    5515 
   Size:        311     504     421     367     385      17    1452      86

Cuts at:   5515    5527    5873    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     504     421     385     367     346     311
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2064    2496    2808    3102    3129     513 
   Size:        552     999     432     312     294      27    3262

  Fragments arranged by size: 

                3262     999     552     432     312     294      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2625    2876     487 
   Size:        683      56    1399     251    3489

  Fragments arranged by size: 

                3489    1399     683     251      56
 
BtsI GCAGTG_nn'

Cuts at:   3110    3394    4188    4208    3110 
   Size:        284     794      20    4780

  Fragments arranged by size: 

                4780     794     284      20
 
ClaI AT'CG_AT

Cuts at:   1554    2934    1554 
   Size:       1380    4498
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2138    2173     529 
   Size:         35    1574      35    4234

  Fragments arranged by size: 

                4234    1574      35      35
 
DraI TTT'AAA

Cuts at:   3182    3182 
   Size:       5878
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4885     809 
   Size:        507    3569    1802

  Fragments arranged by size: 

                3569    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2214    2301    3325 
   Size:         54    1425      50     275      18     384      87    1024

Cuts at:   3325    4214    5843       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425    1024     889     384     275      87      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3325    1537 
   Size:        275    1513    4090

  Fragments arranged by size: 

                4090    1513     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3808    1353 
   Size:       2455    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4465    4839    4985     387 
   Size:       1064     138    2876     374     146    1280

  Fragments arranged by size: 

                2876    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2571    2571 
   Size:       5878
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2893    3941    4538    5325    5593    1239 
   Size:        393    1261    1048     597     787     268    1524

  Fragments arranged by size: 

                1524    1261    1048     787     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2108    2124    2108 
   Size:         16    5862
 
EcoRI G'AATT_C

Cuts at:   3187    3565    3187 
   Size:        378    5500
 
EcoRV GAT'ATC

Cuts at:   2025    2025 
   Size:       5878
 
FspI TGC'GCA

Cuts at:   4384    4384 
   Size:       5878
 
HaeII r_GCGC'y

Cuts at:   1678    2082    3019    5025    1678 
   Size:        404     937    2006    2531

  Fragments arranged by size: 

                2531    2006     937     404
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4519    4551    4593    4625    1373 
   Size:         32     135      32    2947      32      42      32    2626

  Fragments arranged by size: 

                2947    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3222    3468     669 
   Size:        988     281    1284     246    3079

  Fragments arranged by size: 

                3079    1284     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5878
 
HpaI GTT'AAC

Cuts at:   3468    3468 
   Size:       5878
 
KpnI G_GTAC'C

Cuts at:   3203    3203 
   Size:       5878
 
MfeI C'AATT_G

Cuts at:   3477    3477 
   Size:       5878
 
MluI A'CGCG_T

Cuts at:   5754    5754 
   Size:       5878
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2382    4992    5176    1305 
   Size:       1077    2610     184    2007

  Fragments arranged by size: 

                2610    2007    1077     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2216      10 
   Size:         54    1425     343     384    3672

  Fragments arranged by size: 

                3672    1425     384     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2495    2582    2678    2750    3089    3128    3836 
   Size:        648    1329      87      96      72     339      39     708

Cuts at:   3836    4195    4354     518 
   Size:        359     159    2042

  Fragments arranged by size: 

                2042    1329     708     648     359     339     159      96
                  87      72      39
 
MspA1I CmG'CkG

Cuts at:   1606    2268    2913    2979    3294    3971    5119    5364    1606 
   Size:        662     645      66     315     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     662     645     315     245      66
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2496    3102     513 
   Size:        552    1431     606    3289

  Fragments arranged by size: 

                3289    1431     606     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5878
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5878
 
NotI GC'GGCC_GC

Cuts at:   3325    3325 
   Size:       5878
 
NspI r_CATG'y

Cuts at:   1132    1229    2089    2683    3236    4781    1132 
   Size:         97     860     594     553    1545    2229

  Fragments arranged by size: 

                2229    1545     860     594     553      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5878
 
PciI A'CATG_T

Cuts at:   4777    4777 
   Size:       5878
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5878
 
PfoI T'CCnGG_A

Cuts at:   2877    2877 
   Size:       5878
 
PmeI GTTT'AAAC

Cuts at:   3182    3182 
   Size:       5878
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3947    3979    5460    5492    3947 
   Size:         32    1481      32    4333

  Fragments arranged by size: 

                4333    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2124    2124 
   Size:       5878
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5878
 
PsiI TTA'TAA

Cuts at:   3448    3448 
   Size:       5878
 
PspOMI G'GGCC_C

Cuts at:   2108    2108 
   Size:       5878
 
PstI C_TGCA'G

Cuts at:    830    3074    3156    3230     830 
   Size:       2244      82      74    3478

  Fragments arranged by size: 

                3478    2244      82      74
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5878
 
PvuII CAG'CTG

Cuts at:   2268    2913    2979    2268 
   Size:        645      66    5167

  Fragments arranged by size: 

                5167     645      66
 
SacI G_AGCT'C

Cuts at:   2573    2573 
   Size:       5878
 
SalI G'TCGA_C

Cuts at:   1936    3220    1936 
   Size:       1284    4594
 
SbfI CC_TGCA'GG

Cuts at:   3230    3230 
   Size:       5878
 
ScaI AGT'ACT

Cuts at:   4126    5838    4126 
   Size:       1712    4166
 
SfcI C'TryA_G

Cuts at:    826    1045    2533    2968    3070    3152    3226    4361    5042 
   Size:        219    1488     435     102      82      74    1135     681

Cuts at:   5042    5233     826 
   Size:        191    1471

  Fragments arranged by size: 

                1488    1471    1135     681     435     219     191     102
                  82      74
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5878
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5878
 
SmaI CCC'GGG

Cuts at:   1872    3205    1872 
   Size:       1333    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3982    4883    5145     820 
   Size:        197     926    2039     901     262    1553

  Fragments arranged by size: 

                2039    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3669     493 
   Size:       3176    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5878
 
SphI G_CATG'C

Cuts at:   1132    3236    1132 
   Size:       2104    3774
 
SspI AAT'ATT

Cuts at:      5      52    3802       5 
   Size:         47    3750    2081

  Fragments arranged by size: 

                3750    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5878
 
StyI C'CwwG_G

Cuts at:    513    1065    2496    3102    3317     513 
   Size:        552    1431     606     215    3074

  Fragments arranged by size: 

                3074    1431     606     552     215
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2188    2590    3905    3922    4075    4260    4705 
   Size:       1255     608     402    1315      17     153     185     445

Cuts at:   4705     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1315    1255     608     445     402     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2427    2604    4124 
   Size:        274      80      33      51    1443     450     177    1520

Cuts at:   4124    5836      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1520    1443     450     274     177     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5838    5870    5838 
   Size:         32    5846
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5878
 
XbaI T'CTAG_A

Cuts at:   3214    3214 
   Size:       5878
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2658    2658 
   Size:       5878
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5878
 
XmaI C'CCGG_G

Cuts at:   1870    3203    1870 
   Size:       1333    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3084    4007    3084 
   Size:        923    4955
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3686     276 
   Size:         53      83     186     936    2152    2468

  Fragments arranged by size: 

                2468    2152     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI     PvuI
    PvuII     SacI     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AloI     AscI    AvrII     BaeI     BbeI    BfrBI     BplI    BsiWI
  BstZ17I   Bsu36I   DraIII    EcoNI     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PmlI    PspXI
     PsrI    RsrII    SacII    SanDI     SapI    SexAI     SfiI     SfoI
     SrfI     SwaI
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