################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AccIII AclI AcsI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI AspEI AspI AvrII BalI BamHI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BsrI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PstI PvuI RsrII SacI SalI SbfI ScaI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp45I Tsp509I Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 873 GACGTC GACGT^C Acc65I 44 GGTACC G^GTACC Alw44I 1105 GTGCAC G^TGCAC ApaLI 1105 GTGCAC G^TGCAC Asp700I 699 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 44 GGTACC G^GTACC BanII 83 GGGCTC G[GA]GC[CT]^C BbrPI 913 CACGTG CAC^GTG BclI 128 TGATCA T^GATCA BsaAI 913 CACGTG [CT]AC^GT[GA] BsgI 256 GTGCAG GTGCAG(16/14) BsiEI 31 CGGCCG CG[GA][CT]^CG BspHI 458 TCATGA T^CATGA BspMI 1079 ACCTGC ACCTGC(4/8) BssHII 925 GCGCGC G^CGCGC BssSI 306 CACGAG CACGAG(-5/-1) BstAPI 1107 GCACGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1013 CCACCGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 451 TTTAAA TTT^AAA DraIII 913 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 938 CCGCGG C^C[GA][CT]GG EagI 31 CGGCCG C^GGCCG EarI 121 GAAGAG CTCTTC(1/4) EclXI 31 CGGCCG C^GGCCG Eco52I 31 CGGCCG C^GGCCG FbaI 128 TGATCA T^GATCA KpnI 44 GGTACC GGTAC^C Ksp632I 121 GAAGAG CTCTTC(1/4) KspI 938 CCGCGG CCGC^GG MseI 452 TTAA T^TAA MslI 1014 CACCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NotI 30 GCGGCCGC GC^GGCCGC PmaCI 913 CACGTG CAC^GTG PmlI 913 CACGTG CAC^GTG PpuMI 5 AGGACCC [GA]G^G[AT]CC[CT] PshAI 936 GACCGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 458 TCATGA T^CATGA SacII 938 CCGCGG CCGC^GG SapI 121 GAAGAGC GCTCTTC(1/4) SexAI 140 ACCAGGT A^CC[AT]GGT SmaI 40 CCCGGG CCC^GGG StuI 701 AGGCCT AGG^CCT TaqI 26 TCGA T^CGA Tru9I 452 TTAA T^TAA TspRI 254 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 26 TCGA T^CGA XmaCI 40 CCCGGG C^CCGGG XmaI 40 CCCGGG C^CCGGG XmnI 699 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 193 GTAGAC GT^[AC][GT]AC 943 GTCTAC AlwI 201 GATCC GGATC(4/5) 981 GATCC AspHI 1003 GTGCTC G[AT]GC[AT]^C 1105 GTGCAC AvaI 40 CCCGGG C^[CT]CG[GA]G 574 CCCGAG AvaII 6 GGACC G^G[AT]CC 138 GGACC AviII 341 TGCGCA TGC^GCA 890 TGCGCA BbeI 440 GGCGCC GGCGC^C 1033 GGCGCC BlpI 162 GCTGAGC GC^T[ACGT]AGC 276 GCTGAGC Bpu1102I 162 GCTGAGC GC^T[ACGT]AGC 276 GCTGAGC BsiHKAI 1003 GTGCTC G[AT]GC[AT]^C 1105 GTGCAC BsmFI 881 GGGAC GGGAC(10/14) 645 GTCCC BsoBI 40 CCCGGG C^[CT]CG[GA]G 574 CCCGAG BstF5I 264 CATCC GGATG(2/0) 471 GGATG BstYI 200 AGATCC [GA]^GATC[CT] 980 AGATCC CelII 162 GCTGAGC GC^T[ACGT]AGC 276 GCTGAGC DraII 5 AGGACCC [GA]G^G[ACGT]CC[CT] 59 AGGCCCC EaeI 31 CGGCCG [CT]^GGCC[GA] 488 CGGCCA EcoO109I 5 AGGACCC [GA]G^G[ACGT]CC[CT] 59 AGGCCCC FokI 264 CATCC GGATG(9/13) 471 GGATG FspI 341 TGCGCA TGC^GCA 890 TGCGCA HincII 355 GTCAAC GT[CT]^[GA]AC 418 GTCAAC HindII 355 GTCAAC GT[CT]^[GA]AC 418 GTCAAC KasI 440 GGCGCC G^GCGCC 1033 GGCGCC MflI 200 AGATCC [GA]^GATC[CT] 980 AGATCC NarI 440 GGCGCC GG^CGCC 1033 GGCGCC NlaIII 459 CATG CATG^ 819 CATG PvuII 205 CAGCTG CAG^CTG 220 CAGCTG SfaNI 263 GCATC GCATC(5/9) 500 GCATC SfoI 440 GGCGCC GGC^GCC 1033 GGCGCC XhoII 200 AGATCC [GA]^GATC[CT] 980 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AcyI AfaI AluI BanI BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiYI BslI Bsp1286I BsrBI BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIV PleI RsaI Sau3AI Sau96I ScrFI SmlI StyI TseI XcmI