(Circular) (Six-base) MAPSORT of: pFN21AB8989.seq Check: 6181 from: 1 to: 5875 pFN21AB8989 5875 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2882 3685 278 Size: 53 83 186 936 1346 803 2468 Fragments arranged by size: 2468 1346 936 803 186 83 53 AccI GT'mk_AC Cuts at: 1937 2199 2949 3218 1937 Size: 262 750 269 4594 Fragments arranged by size: 4594 750 269 262 Acc65I G'GTAC_C Cuts at: 2049 3196 2049 Size: 1147 4728 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2894 3938 5322 5590 1632 Size: 1262 1044 1384 268 1917 Fragments arranged by size: 1917 1384 1262 1044 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 4774 5751 1203 Size: 3571 977 1327 Fragments arranged by size: 3571 1327 977 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2696 1184 Size: 1512 4363 AlwNI CAG_nnn'CTG Cuts at: 3618 5190 3618 Size: 1572 4303 ApaLI G'TGCA_C Cuts at: 3110 3932 5088 3110 Size: 822 1156 3897 Fragments arranged by size: 3897 1156 822 ApoI r'AATT_y Cuts at: 1493 3184 3410 3562 5671 1493 Size: 1691 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 226 152 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 2045 2579 3200 1870 Size: 73 102 534 621 4545 Fragments arranged by size: 4545 621 534 102 73 BamHI G'GATC_C Cuts at: 3205 3572 3205 Size: 367 5508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2049 2445 2848 2956 3038 Size: 325 217 627 262 396 403 108 82 Cuts at: 3038 3196 4651 618 Size: 158 1455 1842 Fragments arranged by size: 1842 1455 627 403 396 325 262 217 158 108 82 BanII G_rGCy'C Cuts at: 1391 2092 3194 1391 Size: 701 1102 4072 Fragments arranged by size: 4072 1102 701 BbeI G_GCGC'C Cuts at: 2449 3042 2449 Size: 593 5282 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2375 2738 2771 2804 2882 928 Size: 971 476 363 33 33 78 3921 Fragments arranged by size: 3921 971 476 363 78 33 33 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4066 4100 1903 Size: 34 2129 34 3678 Fragments arranged by size: 3678 2129 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3767 4977 3767 Size: 1210 4665 BclI T'GATC_A Cuts at: 1548 2133 1548 Size: 585 5290 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4486 136 Size: 107 122 71 631 605 2814 1525 Fragments arranged by size: 2814 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5870 5870 Size: 5875 BlpI GC'TnA_GC Cuts at: 2168 2282 3292 2168 Size: 114 1010 4751 Fragments arranged by size: 4751 1010 114 Bme1580I G_kGCm'C Cuts at: 1790 2840 2853 3114 3936 5092 1790 Size: 1050 13 261 822 1156 2573 Fragments arranged by size: 2573 1156 1050 822 261 13 BmgBI CAC'GTC Cuts at: 1318 2977 3109 1318 Size: 1659 132 4084 Fragments arranged by size: 4084 1659 132 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3606 4564 442 Size: 416 53 83 399 24 2189 958 1753 Fragments arranged by size: 2189 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2095 2095 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2357 2408 2438 2621 2780 2813 2834 Size: 1010 108 51 30 183 159 33 21 Cuts at: 2834 3143 4535 1239 Size: 309 1392 2579 Fragments arranged by size: 2579 1392 1010 309 183 159 108 51 33 30 21 Bpu10I CC'TnA_GC Cuts at: 2091 2091 Size: 5875 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2202 2759 2996 4000 4865 5163 2202 Size: 557 237 1004 865 298 2914 Fragments arranged by size: 2914 1004 865 557 298 237 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4538 882 Size: 617 3039 2219 Fragments arranged by size: 3039 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2920 3666 493 Size: 711 1716 746 2702 Fragments arranged by size: 2702 1716 746 711 BsaBI GATnn'nnATC Cuts at: 5869 5869 Size: 5875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2058 2446 2879 3039 Size: 53 83 186 936 525 388 433 160 Cuts at: 3039 3144 3682 4064 4699 275 Size: 105 538 382 635 1451 Fragments arranged by size: 1451 936 635 538 525 433 388 382 186 160 105 83 53 BsaWI w'CCGG_w Cuts at: 4308 4706 4980 5127 5538 4308 Size: 398 274 147 411 4645 Fragments arranged by size: 4645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2093 2123 2594 2624 2918 Size: 30 165 30 690 30 471 30 294 Cuts at: 2918 2948 1178 Size: 30 4105 Fragments arranged by size: 4105 690 471 294 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2246 2570 2597 2648 2660 3149 1186 Size: 931 129 324 27 51 12 489 3912 Fragments arranged by size: 3912 931 489 324 129 51 27 12 BseYI C'CCAG_C Cuts at: 2084 2290 2560 2833 5078 5574 2084 Size: 206 270 273 2245 496 2385 Fragments arranged by size: 2385 2245 496 273 270 206 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2282 1163 Size: 573 546 4756 Fragments arranged by size: 4756 573 546 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2039 3325 4086 5114 1540 Size: 275 186 38 1286 761 1028 2301 Fragments arranged by size: 2301 1286 1028 761 275 186 38 BsiHKAI G_wGCw'C Cuts at: 3012 3114 3936 4021 5092 3012 Size: 102 822 85 1071 3795 Fragments arranged by size: 3795 1071 822 102 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2092 2840 2853 3012 3114 3194 3936 Size: 399 302 748 13 159 102 80 742 Cuts at: 3936 4021 5092 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 748 742 399 302 159 102 85 80 13 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 2463 3764 5494 2463 Size: 1301 1730 2844 Fragments arranged by size: 2844 1730 1301 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3093 3230 844 Size: 2249 137 3489 Fragments arranged by size: 3489 2249 137 BsrBI CCG'CTC Cuts at: 1394 1823 2035 2236 2575 2860 3762 1394 Size: 429 212 201 339 285 902 3507 Fragments arranged by size: 3507 902 429 339 285 212 201 BsrDI GCAATG_nn' Cuts at: 65 1248 4370 4544 65 Size: 1183 3122 174 1396 Fragments arranged by size: 3122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2117 2491 3077 4519 4706 1700 Size: 110 307 374 586 1442 187 2869 Fragments arranged by size: 2869 1442 586 374 307 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5875 BssHII G'CGCG_C Cuts at: 1925 2930 1925 Size: 1005 4870 BssSI C'ACGA_G Cuts at: 2311 3935 4947 2311 Size: 1624 1012 3239 Fragments arranged by size: 3239 1624 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3118 794 Size: 2324 3551 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5875 BstXI CCAn_nnnn'nTGG Cuts at: 3025 3025 Size: 5875 BstYI r'GATC_y Cuts at: 1972 2205 2985 3205 3572 3957 3974 5426 5512 Size: 233 780 220 367 385 17 1452 86 Cuts at: 5512 5524 5870 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 780 385 367 346 233 220 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2943 513 Size: 552 939 939 3445 Fragments arranged by size: 3445 939 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 2265 3391 4185 4205 2265 Size: 1126 794 20 3935 Fragments arranged by size: 3935 1126 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5840 DraI TTT'AAA Cuts at: 2458 3179 2458 Size: 721 5154 DraIII CAC_nnn'GTG Cuts at: 2923 2923 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2036 2493 3322 Size: 54 1425 50 275 18 206 457 829 Cuts at: 3322 4211 5840 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 829 457 275 206 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2036 3322 1537 Size: 275 224 1286 4090 Fragments arranged by size: 4090 1286 275 224 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 3805 1353 Size: 768 1684 3423 Fragments arranged by size: 3423 1684 768 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2258 4462 4836 4982 387 Size: 1064 138 669 2204 374 146 1280 Fragments arranged by size: 2204 1280 1064 669 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2357 2408 2438 2621 2780 2813 Size: 393 617 108 51 30 183 159 33 Cuts at: 2813 2834 2894 3143 3938 4535 5322 5590 1239 Size: 21 60 249 795 597 787 268 1524 Fragments arranged by size: 1524 795 787 617 597 393 268 249 183 159 108 60 51 33 30 21 EcoO109I rG'GnC_Cy Cuts at: 2011 2065 2011 Size: 54 5821 EcoRI G'AATT_C Cuts at: 3184 3562 3184 Size: 378 5497 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2725 2725 Size: 5875 FspI TGC'GCA Cuts at: 2348 2897 4381 2348 Size: 549 1484 3842 Fragments arranged by size: 3842 1484 549 HaeII r_GCGC'y Cuts at: 1678 2395 2449 2537 2600 3042 5022 1678 Size: 717 54 88 63 442 1980 2531 Fragments arranged by size: 2531 1980 717 442 88 63 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 2594 2626 4516 Size: 32 135 32 521 32 469 32 1890 Cuts at: 4516 4548 4590 4622 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1890 521 469 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2362 2425 3219 3465 669 Size: 988 281 424 63 794 246 3079 Fragments arranged by size: 3079 988 794 424 281 246 63 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KasI G'GCGC_C Cuts at: 2445 3038 2445 Size: 593 5282 KpnI G_GTAC'C Cuts at: 2053 3200 2053 Size: 1147 4728 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2121 2508 2592 3120 4989 5173 1305 Size: 816 387 84 528 1869 184 2007 Fragments arranged by size: 2007 1869 816 528 387 184 84 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4053 Fragments arranged by size: 4053 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3023 3833 4192 4351 518 Size: 648 1857 810 359 159 2042 Fragments arranged by size: 2042 1857 810 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2084 2099 2212 2227 2594 2676 2945 3291 Size: 478 15 113 15 367 82 269 346 Cuts at: 3291 3968 5116 5361 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 478 367 346 269 245 113 82 15 15 NaeI GCC'GGC Cuts at: 2119 2493 3079 2119 Size: 374 586 4915 Fragments arranged by size: 4915 586 374 NarI GG'CG_CC Cuts at: 2446 3039 2446 Size: 593 5282 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4384 Fragments arranged by size: 4384 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5875 NgoMIV G'CCGG_C Cuts at: 2117 2491 3077 2117 Size: 374 586 4915 Fragments arranged by size: 4915 586 374 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 2036 3322 2036 Size: 1286 4589 NspI r_CATG'y Cuts at: 1132 1229 3233 4778 1132 Size: 97 2004 1545 2229 Fragments arranged by size: 2229 2004 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5875 PfoI T'CCnGG_A Cuts at: 2175 2175 Size: 5875 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PmlI CAC'GTG Cuts at: 2920 2920 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2011 2011 Size: 5875 PshAI GACnn'nnGTC Cuts at: 1613 2945 1613 Size: 1332 4543 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PstI C_TGCA'G Cuts at: 830 3227 830 Size: 2397 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 PvuII CAG'CTG Cuts at: 2212 2227 2212 Size: 15 5860 SacII CC_GC'GG Cuts at: 2946 2946 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SapI GCTCTTCn'nnn_ Cuts at: 2121 2121 Size: 5875 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SexAI A'CCwGG_T Cuts at: 2145 2145 Size: 5875 SfcI C'TryA_G Cuts at: 826 1045 3223 4358 5039 5230 826 Size: 219 2178 1135 681 191 1471 Fragments arranged by size: 2178 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2447 3040 2447 Size: 593 5282 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 2047 3202 1872 Size: 175 1155 4545 Fragments arranged by size: 4545 1155 175 SmlI C'TyrA_G Cuts at: 820 1017 1943 2217 2774 3011 3979 4880 5142 Size: 197 926 274 557 237 968 901 262 Cuts at: 5142 820 Size: 1553 Fragments arranged by size: 1553 968 926 901 557 274 262 237 197 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 3233 1132 Size: 2101 3774 SspI AAT'ATT Cuts at: 5 52 3799 5 Size: 47 3747 2081 Fragments arranged by size: 3747 2081 47 StuI AGG'CCT Cuts at: 1517 2708 1517 Size: 1191 4684 StyI C'CwwG_G Cuts at: 513 1065 2004 2715 2736 2781 3314 513 Size: 552 939 711 21 45 533 3074 Fragments arranged by size: 3074 939 711 552 533 45 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3902 3919 4072 4257 4702 325 Size: 1255 2322 17 153 185 445 1498 Fragments arranged by size: 2322 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4121 5833 96 Size: 274 80 33 51 1443 2144 1712 138 Fragments arranged by size: 2144 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5835 5867 5835 Size: 32 5843 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2722 2788 3025 2722 Size: 66 237 5572 Fragments arranged by size: 5572 237 66 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 2045 3200 1870 Size: 175 1155 4545 Fragments arranged by size: 4545 1155 175 XmnI GAAnn'nnTTC Cuts at: 2708 4004 2708 Size: 1296 4579 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2880 3683 276 Size: 53 83 186 936 1346 803 2468 Fragments arranged by size: 2468 1346 936 803 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SanDI SfiI SrfI SwaI