(Circular) (Six-base) MAPSORT of: pFN21AB8984.seq Check: 8947 from: 1 to: 5863 pFN21AB8984 5863 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3061 3061 Size: 5863 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3673 278 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3206 1937 Size: 1269 4594 Acc65I G'GTAC_C Cuts at: 3184 3184 Size: 5863 AclI AA'CG_TT Cuts at: 1563 3990 4363 1563 Size: 2427 373 3063 Fragments arranged by size: 3063 2427 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3926 5310 5578 1632 Size: 2294 1384 268 1917 Fragments arranged by size: 2294 1917 1384 268 AfeI AGC'GCT Cuts at: 2354 2354 Size: 5863 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5666 AflIII A'CryG_T Cuts at: 1203 4762 5739 1203 Size: 3559 977 1327 Fragments arranged by size: 3559 1327 977 AgeI A'CCGG_T Cuts at: 4694 4694 Size: 5863 AhdI GACnn_n'nnGTC Cuts at: 4592 4592 Size: 5863 AleI CACnn'nnGTG Cuts at: 1166 2033 1166 Size: 867 4996 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5863 AlwNI CAG_nnn'CTG Cuts at: 2634 3606 5178 2634 Size: 972 1572 3319 Fragments arranged by size: 3319 1572 972 ApaI G_GGCC'C Cuts at: 2403 2536 3010 2403 Size: 133 474 5256 Fragments arranged by size: 5256 474 133 ApaLI G'TGCA_C Cuts at: 3920 5076 3920 Size: 1156 4707 ApoI r'AATT_y Cuts at: 1493 3172 3398 3550 5659 1493 Size: 1679 226 152 2109 1697 Fragments arranged by size: 2109 1697 1679 226 152 AscI GG'CGCG_CC Cuts at: 2135 2135 Size: 5863 AseI AT'TA_AT Cuts at: 160 1032 4417 160 Size: 872 3385 1606 Fragments arranged by size: 3385 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 AvaI C'yCGr_G Cuts at: 1870 1943 2259 2939 3188 1870 Size: 73 316 680 249 4545 Fragments arranged by size: 4545 680 316 249 73 BamHI G'GATC_C Cuts at: 3193 3560 3193 Size: 367 5496 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2192 2546 3184 4639 618 Size: 325 217 627 405 354 638 1455 1842 Fragments arranged by size: 1842 1455 638 627 405 354 325 217 BanII G_rGCy'C Cuts at: 1391 2045 2210 2403 2536 2672 3010 3081 3182 Size: 654 165 193 133 136 338 71 101 Cuts at: 3182 1391 Size: 4072 Fragments arranged by size: 4072 654 338 193 165 136 133 101 71 BbeI G_GCGC'C Cuts at: 2550 2550 Size: 5863 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2869 2893 928 Size: 971 970 24 3898 Fragments arranged by size: 3898 971 970 24 BbvCI CC'TCA_GC Cuts at: 2627 2627 Size: 5863 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4054 4088 1903 Size: 34 2117 34 3678 Fragments arranged by size: 3678 2117 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3755 4965 3755 Size: 1210 4653 BclI T'GATC_A Cuts at: 1548 1548 Size: 5863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2417 2667 4474 Size: 107 122 71 631 605 745 250 1807 Cuts at: 4474 136 Size: 1525 Fragments arranged by size: 1807 1525 745 631 605 250 122 107 71 BglII A'GATC_T Cuts at: 5858 5858 Size: 5863 BlpI GC'TnA_GC Cuts at: 2366 3280 2366 Size: 914 4949 Bme1580I G_kGCm'C Cuts at: 1790 2195 2403 2536 3010 3924 5080 1790 Size: 405 208 133 474 914 1156 2573 Fragments arranged by size: 2573 1156 914 474 405 208 133 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5863 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3594 4552 442 Size: 416 53 83 399 24 2177 958 1753 Fragments arranged by size: 2177 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5863 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4523 1239 Size: 3284 2579 Bpu10I CC'TnA_GC Cuts at: 2117 2204 2627 2117 Size: 87 423 5353 Fragments arranged by size: 5353 423 87 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2897 3988 4853 5151 2897 Size: 1091 865 298 3609 Fragments arranged by size: 3609 1091 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4526 882 Size: 617 3027 2219 Fragments arranged by size: 3027 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2035 3654 493 Size: 711 831 1619 2702 Fragments arranged by size: 2702 1619 831 711 BsaBI GATnn'nnATC Cuts at: 5857 5857 Size: 5863 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2318 2547 3670 4052 Size: 53 83 186 936 785 229 1123 382 Cuts at: 4052 4687 275 Size: 635 1451 Fragments arranged by size: 1451 1123 936 785 635 382 229 186 83 53 BsaWI w'CCGG_w Cuts at: 2165 2693 4296 4694 4968 5115 5526 2165 Size: 528 1603 398 274 147 411 2502 Fragments arranged by size: 2502 1603 528 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5638 Fragments arranged by size: 5638 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3007 1186 Size: 1821 4042 BseYI C'CCAG_C Cuts at: 2188 2248 2470 2529 2535 2976 5066 5562 2188 Size: 60 222 59 6 441 2090 496 2489 Fragments arranged by size: 2489 2090 496 441 222 60 59 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2038 1163 Size: 573 302 4988 Fragments arranged by size: 4988 573 302 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2132 2214 2687 3313 4074 5102 Size: 275 186 131 82 473 626 761 1028 Cuts at: 5102 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 626 473 275 186 131 82 BsiHKAI G_wGCw'C Cuts at: 2759 3924 4009 5080 2759 Size: 1165 85 1071 3542 Fragments arranged by size: 3542 1165 1071 85 BsmI GAATG_Cn' Cuts at: 3374 3467 3374 Size: 93 5770 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2195 2210 2403 2536 2672 2759 Size: 399 255 150 15 193 133 136 87 Cuts at: 2759 3010 3081 3182 3924 4009 5080 1391 Size: 251 71 101 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 255 251 193 150 136 133 101 87 85 71 15 BspEI T'CCGG_A Cuts at: 5526 5526 Size: 5863 BspHI T'CATG_A Cuts at: 2733 3752 5482 2733 Size: 1019 1730 3114 Fragments arranged by size: 3114 1730 1019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2193 3061 3218 844 Size: 1349 868 157 3489 Fragments arranged by size: 3489 1349 868 157 BsrBI CCG'CTC Cuts at: 1394 1823 3750 1394 Size: 429 1927 3507 Fragments arranged by size: 3507 1927 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4358 4532 65 Size: 1183 3110 174 1396 Fragments arranged by size: 3110 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2053 2081 2131 2570 4507 4694 1700 Size: 110 243 28 50 439 1937 187 2869 Fragments arranged by size: 2869 1937 439 243 187 110 50 28 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5863 BssHII G'CGCG_C Cuts at: 1925 2135 2231 2457 1925 Size: 210 96 226 5331 Fragments arranged by size: 5331 226 210 96 BssSI C'ACGA_G Cuts at: 3923 4935 3923 Size: 1012 4851 BstAPI GCAn_nnn'nTGC Cuts at: 794 2511 794 Size: 1717 4146 BstBI TT'CG_AA Cuts at: 4667 4667 Size: 5863 BstEII G'GTnAC_C Cuts at: 1184 2161 2620 1184 Size: 977 459 4427 Fragments arranged by size: 4427 977 459 BstXI CCAn_nnnn'nTGG Cuts at: 2347 2347 Size: 5863 BstYI r'GATC_y Cuts at: 1972 3193 3560 3945 3962 5414 5500 5512 5858 Size: 1221 367 385 17 1452 86 12 346 Cuts at: 5858 1972 Size: 1977 Fragments arranged by size: 1977 1452 1221 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2340 2346 2681 2950 3114 513 Size: 552 939 336 6 335 269 164 3262 Fragments arranged by size: 3262 939 552 336 335 269 164 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2295 487 Size: 683 56 1069 4055 Fragments arranged by size: 4055 1069 683 56 BtsI GCAGTG_nn' Cuts at: 2818 3379 4173 4193 2818 Size: 561 794 20 4488 Fragments arranged by size: 4488 794 561 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5863 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5828 DraI TTT'AAA Cuts at: 3167 3167 Size: 5863 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2873 4870 809 Size: 507 1557 1997 1802 Fragments arranged by size: 1997 1802 1557 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2079 2175 Size: 54 1425 50 275 18 225 24 96 Cuts at: 2175 2211 2343 2684 2748 3310 4199 5828 8 Size: 36 132 341 64 562 889 1629 43 Fragments arranged by size: 1629 1425 889 562 341 275 225 132 96 64 54 50 43 36 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2211 2684 3310 1537 Size: 275 399 473 626 4090 Fragments arranged by size: 4090 626 473 399 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2456 3793 1353 Size: 1103 1337 3423 Fragments arranged by size: 3423 1337 1103 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4450 4824 4970 387 Size: 1064 138 435 2426 374 146 1280 Fragments arranged by size: 2426 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3926 4523 5310 5578 1239 Size: 393 2294 597 787 268 1524 Fragments arranged by size: 2294 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2989 2989 Size: 5863 EcoO109I rG'GnC_Cy Cuts at: 2400 2824 2998 3006 3007 2400 Size: 424 174 8 1 5256 Fragments arranged by size: 5256 424 174 8 1 EcoRI G'AATT_C Cuts at: 3172 3550 3172 Size: 378 5485 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2917 2917 Size: 5863 FseI GG_CCGG'CC Cuts at: 2057 2057 Size: 5863 FspI TGC'GCA Cuts at: 4369 4369 Size: 5863 HaeII r_GCGC'y Cuts at: 1678 2356 2366 2423 2550 2662 3114 5010 1678 Size: 678 10 57 127 112 452 1896 2531 Fragments arranged by size: 2531 1896 678 452 127 112 57 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2901 2933 4504 4536 4578 Size: 32 135 32 1329 32 1571 32 42 Cuts at: 4578 4610 1373 Size: 32 2626 Fragments arranged by size: 2626 1571 1329 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3207 3453 669 Size: 988 281 1269 246 3079 Fragments arranged by size: 3079 1269 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5863 HpaI GTT'AAC Cuts at: 3453 3453 Size: 5863 KasI G'GCGC_C Cuts at: 2546 2546 Size: 5863 KpnI G_GTAC'C Cuts at: 3188 3188 Size: 5863 MfeI C'AATT_G Cuts at: 3462 3462 Size: 5863 MluI A'CGCG_T Cuts at: 5739 5739 Size: 5863 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4977 5161 1305 Size: 3672 184 2007 Fragments arranged by size: 3672 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4041 Fragments arranged by size: 4041 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2033 2069 2345 2738 2750 3821 4180 Size: 648 867 36 276 393 12 1071 359 Cuts at: 4180 4339 518 Size: 159 2042 Fragments arranged by size: 2042 1071 867 648 393 359 276 159 36 12 MspA1I CmG'CkG Cuts at: 1606 2392 2406 2508 2778 3105 3279 3956 5104 Size: 786 14 102 270 327 174 677 1148 Cuts at: 5104 5349 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 786 677 327 270 245 174 102 14 NaeI GCC'GGC Cuts at: 2055 2572 2055 Size: 517 5346 NarI GG'CG_CC Cuts at: 2547 2547 Size: 5863 NcoI C'CATG_G Cuts at: 513 1065 2004 2340 513 Size: 552 939 336 4036 Fragments arranged by size: 4036 939 552 336 NdeI CA'TA_TG Cuts at: 387 387 Size: 5863 NgoMIV G'CCGG_C Cuts at: 2053 2570 2053 Size: 517 5346 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5863 NotI GC'GGCC_GC Cuts at: 3310 3310 Size: 5863 NspI r_CATG'y Cuts at: 1132 1229 2527 2644 3221 4766 1132 Size: 97 1298 117 577 1545 2229 Fragments arranged by size: 2229 1545 1298 577 117 97 PasI CC'CwG_GG Cuts at: 1348 2107 2871 1348 Size: 759 764 4340 Fragments arranged by size: 4340 764 759 PciI A'CATG_T Cuts at: 4762 4762 Size: 5863 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5863 PfoI T'CCnGG_A Cuts at: 2862 2987 2862 Size: 125 5738 PmeI GTTT'AAAC Cuts at: 3167 3167 Size: 5863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3932 3964 5445 5477 3932 Size: 32 1481 32 4318 Fragments arranged by size: 4318 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2824 2998 2824 Size: 174 5689 PshAI GACnn'nnGTC Cuts at: 1613 2741 1613 Size: 1128 4735 PsiI TTA'TAA Cuts at: 3433 3433 Size: 5863 PspOMI G'GGCC_C Cuts at: 2399 2532 3006 2399 Size: 133 474 5256 Fragments arranged by size: 5256 474 133 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2015 2047 2015 Size: 32 5831 PstI C_TGCA'G Cuts at: 830 3215 830 Size: 2385 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5863 PvuII CAG'CTG Cuts at: 2392 2508 2392 Size: 116 5747 SalI G'TCGA_C Cuts at: 1936 3205 1936 Size: 1269 4594 SanDI GG'GwC_CC Cuts at: 2998 2998 Size: 5863 SbfI CC_TGCA'GG Cuts at: 3215 3215 Size: 5863 ScaI AGT'ACT Cuts at: 2495 4111 5823 2495 Size: 1616 1712 2535 Fragments arranged by size: 2535 1712 1616 SfcI C'TryA_G Cuts at: 826 1045 3211 4346 5027 5218 826 Size: 219 2166 1135 681 191 1471 Fragments arranged by size: 2166 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2548 2548 Size: 5863 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5863 SmaI CCC'GGG Cuts at: 1872 3190 1872 Size: 1318 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2912 3967 4868 5130 820 Size: 197 926 969 1055 901 262 1553 Fragments arranged by size: 1553 1055 969 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3654 493 Size: 3161 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5863 SphI G_CATG'C Cuts at: 1132 2527 3221 1132 Size: 1395 694 3774 Fragments arranged by size: 3774 1395 694 SspI AAT'ATT Cuts at: 5 52 3787 5 Size: 47 3735 2081 Fragments arranged by size: 3735 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5863 StyI C'CwwG_G Cuts at: 513 1065 2004 2340 3002 3302 513 Size: 552 939 336 662 300 3074 Fragments arranged by size: 3074 939 662 552 336 300 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2608 2801 3890 3907 4060 4245 4690 Size: 1255 1028 193 1089 17 153 185 445 Cuts at: 4690 325 Size: 1498 Fragments arranged by size: 1498 1255 1089 1028 445 193 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2493 4109 5821 Size: 274 80 33 51 1443 516 1616 1712 Cuts at: 5821 96 Size: 138 Fragments arranged by size: 1712 1616 1443 516 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5823 5855 5823 Size: 32 5831 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5863 XbaI T'CTAG_A Cuts at: 3199 3199 Size: 5863 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 2185 Size: 5863 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5863 XmaI C'CCGG_G Cuts at: 1870 3188 1870 Size: 1318 4545 XmnI GAAnn'nnTTC Cuts at: 3992 3992 Size: 5863 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3671 276 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FspAI NruI NsiI PacI PmlI PspXI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI