Restriction map for
pFN21AB8979
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII AccI AccII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspHI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KspI MaeI MaeII MamI MfeI
MluI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 257 GGTACC G^GTACC
AccIII 857 TCCGGA T^CCGGA
AciI 570 CCGC CCGC(-3/-1)
AcsI 135 AAATTC [GA]^AATT[CT]
AflII 273 CTTAAG C^TTAAG
AlwNI 20 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 135 AAATTC [GA]^AATT[CT]
Asp718I 257 GGTACC G^GTACC
AspHI 125 GAGCTC G[AT]GC[AT]^C
BanI 257 GGTACC G^G[CT][GA]CC
BanII 125 GAGCTC G[GA]GC[CT]^C
BfrI 273 CTTAAG C^TTAAG
BsaWI 857 TCCGGA [AT]^CCGG[AT]
BseAI 857 TCCGGA T^CCGGA
BseRI 910 CTCCTC GAGGAG(10/8)
BsiHKAI 125 GAGCTC G[AT]GC[AT]^C
BsmAI 908 GTCTC GTCTC(1/5)
BsmFI 325 GTCCC GGGAC(10/14)
BsmI 799 GCATTC GAATGC(1/-1)
BspEI 857 TCCGGA T^CCGGA
DdeI 1056 CTTAG C^T[ACGT]AG
DsaI 251 CCATGG C^C[GA][CT]GG
EarI 145 GAAGAG CTCTTC(1/4)
EcoT22I 869 ATGCAT ATGCA^T
KpnI 257 GGTACC GGTAC^C
Ksp632I 145 GAAGAG CTCTTC(1/4)
MroI 857 TCCGGA T^CCGGA
MspA1I 20 CAGCTG C[AC]G^C[GT]G
NcoI 251 CCATGG C^CATGG
NsiI 869 ATGCAT ATGCA^T
PpuMI 488 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 20 CAGCTG CAG^CTG
SacI 125 GAGCTC GAGCT^C
ScaI 277 AGTACT AGT^ACT
SmlI 273 CTTAAG C^T[CT][GA]AG
TfiI 809 GATTC G^A[AT]TC
Tsp45I 340 GTGAC ^GT[GC]AC
XcmI 296 CCATCTACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AflIII 527 ACATGT A^C[GA][CT]GT
637 ACATGT
AlwI 371 GATCC GGATC(4/5)
1060 GATCC
AvaII 365 GGACC G^G[AT]CC
489 GGTCC
BalI 404 TGGCCA TGG^CCA
578 TGGCCA
BmyI 125 GAGCTC G[AGT]GC[ACT]^C
207 GTGCCC
Bsp1286I 125 GAGCTC G[AGT]GC[ACT]^C
207 GTGCCC
BspLU11I 527 ACATGT A^CATGT
637 ACATGT
BspMI 291 ACCTGC ACCTGC(4/8)
730 ACCTGC
BsrDI 408 CATTGC GCAATG(2/0)
434 CATTGC
BsrI 473 ACTGG ACTGG(1/-1)
645 ACTGG
BstYI 370 AGATCC [GA]^GATC[CT]
1059 AGATCC
DraII 488 AGGTCCT [GA]G^G[ACGT]CC[CT]
605 GGGGCCT
EcoO109I 488 AGGTCCT [GA]G^G[ACGT]CC[CT]
605 GGGGCCT
MflI 370 AGATCC [GA]^GATC[CT]
1059 AGATCC
MluNI 404 TGGCCA TGG^CCA
578 TGGCCA
MscI 404 TGGCCA TGG^CCA
578 TGGCCA
MwoI 36 GCTTCTGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
563 GCATCAACCGC
NspI 527 ACATGT [GA]CATG^[CT]
637 ACATGT
PleI 757 GAGTC GAGTC(4/5)
906 GAGTC
SspI 701 AATATT AAT^ATT
952 AATATT
XhoII 370 AGATCC [GA]^GATC[CT]
1059 AGATCC
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AfaI AluI BbvI BcnI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cac8I Cfr13I DpnII EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI StyI Tru9I TseI Tsp509I
TspRI