(Circular) (Six-base) MAPSORT of: pFN21AB8979.seq Check: 3496 from: 1 to: 5827 pFN21AB8979 5827 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2744 2744 Size: 5827 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3637 278 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3170 1937 Size: 1233 4594 Acc65I G'GTAC_C Cuts at: 2262 3148 2262 Size: 886 4941 AclI AA'CG_TT Cuts at: 1563 3954 4327 1563 Size: 2391 373 3063 Fragments arranged by size: 3063 2391 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2238 2350 2717 3890 5274 5542 1632 Size: 606 112 367 1173 1384 268 1917 Fragments arranged by size: 1917 1384 1173 606 367 268 112 AflII C'TTAA_G Cuts at: 820 1017 2278 820 Size: 197 1261 4369 Fragments arranged by size: 4369 1261 197 AflIII A'CryG_T Cuts at: 1203 2532 2642 4726 5703 1203 Size: 1329 110 2084 977 1327 Fragments arranged by size: 2084 1329 1327 977 110 AgeI A'CCGG_T Cuts at: 4658 4658 Size: 5827 AhdI GACnn_n'nnGTC Cuts at: 4556 4556 Size: 5827 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5827 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2646 1184 Size: 1462 4365 AlwNI CAG_nnn'CTG Cuts at: 2030 3570 5142 2030 Size: 1540 1572 2715 Fragments arranged by size: 2715 1572 1540 ApaLI G'TGCA_C Cuts at: 3884 5040 3884 Size: 1156 4671 ApoI r'AATT_y Cuts at: 1493 2140 3136 3362 3514 5623 1493 Size: 647 996 226 152 2109 1697 Fragments arranged by size: 2109 1697 996 647 226 152 AseI AT'TA_AT Cuts at: 160 1032 4381 160 Size: 872 3349 1606 Fragments arranged by size: 3349 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 AvaI C'yCGr_G Cuts at: 1870 1943 3152 1870 Size: 73 1209 4545 Fragments arranged by size: 4545 1209 73 BamHI G'GATC_C Cuts at: 3157 3524 3157 Size: 367 5460 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2262 3148 4603 618 Size: 325 217 627 475 886 1455 1842 Fragments arranged by size: 1842 1455 886 627 475 325 217 BanII G_rGCy'C Cuts at: 1391 2134 3146 1391 Size: 743 1012 4072 Fragments arranged by size: 4072 1012 743 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4856 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4018 4052 1903 Size: 34 2081 34 3678 Fragments arranged by size: 3678 2081 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3719 4929 3719 Size: 1210 4617 BclI T'GATC_A Cuts at: 1548 1548 Size: 5827 BfrBI ATG'CAT Cuts at: 2876 2876 Size: 5827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4438 136 Size: 107 122 71 631 605 2766 1525 Fragments arranged by size: 2766 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5822 5822 Size: 5827 BlpI GC'TnA_GC Cuts at: 3244 3244 Size: 5827 Bme1580I G_kGCm'C Cuts at: 1790 2216 3888 5044 1790 Size: 426 1672 1156 2573 Fragments arranged by size: 2573 1672 1156 426 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5827 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2488 3558 4516 Size: 416 53 83 399 24 1071 1070 958 Cuts at: 4516 442 Size: 1753 Fragments arranged by size: 1753 1071 1070 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5827 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4487 1239 Size: 3248 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3952 4817 5115 3952 Size: 865 298 4664 Fragments arranged by size: 4664 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4490 882 Size: 617 2991 2219 Fragments arranged by size: 2991 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3618 493 Size: 711 2414 2702 Fragments arranged by size: 2702 2414 711 BsaBI GATnn'nnATC Cuts at: 5821 5821 Size: 5827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3634 4016 4651 275 Size: 53 83 186 936 2101 382 635 1451 Fragments arranged by size: 2101 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2862 4260 4658 4932 5079 5490 2862 Size: 1398 398 274 147 411 3199 Fragments arranged by size: 3199 1398 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2780 2810 1178 Size: 30 165 30 1377 30 4195 Fragments arranged by size: 4195 1377 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2906 1186 Size: 1720 4107 BseYI C'CCAG_C Cuts at: 5030 5526 5030 Size: 496 5331 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5254 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3277 4038 5066 1540 Size: 275 186 1276 761 1028 2301 Fragments arranged by size: 2301 1276 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2134 3888 3973 5044 2134 Size: 1754 85 1071 2917 Fragments arranged by size: 2917 1754 1071 85 BsmI GAATG_Cn' Cuts at: 2804 3338 3431 2804 Size: 534 93 5200 Fragments arranged by size: 5200 534 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2134 2216 3146 3888 3973 5044 1391 Size: 399 344 82 930 742 85 1071 2174 Fragments arranged by size: 2174 1071 930 742 399 344 85 82 BspEI T'CCGG_A Cuts at: 2862 5490 2862 Size: 2628 3199 BspHI T'CATG_A Cuts at: 3716 5446 3716 Size: 1730 4097 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2305 2744 3182 844 Size: 1461 439 438 3489 Fragments arranged by size: 3489 1461 439 438 BsrBI CCG'CTC Cuts at: 1394 1823 3714 1394 Size: 429 1891 3507 Fragments arranged by size: 3507 1891 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2412 2438 4322 4496 65 Size: 1183 1164 26 1884 174 1396 Fragments arranged by size: 1884 1396 1183 1164 174 26 BsrFI r'CCGG_y Cuts at: 1700 1810 4471 4658 1700 Size: 110 2661 187 2869 Fragments arranged by size: 2869 2661 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5827 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5827 BssSI C'ACGA_G Cuts at: 3887 4899 3887 Size: 1012 4815 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5827 BstBI TT'CG_AA Cuts at: 4631 4631 Size: 5827 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5827 BstYI r'GATC_y Cuts at: 1972 2375 3064 3157 3524 3909 3926 5378 5464 Size: 403 689 93 367 385 17 1452 86 Cuts at: 5464 5476 5822 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 689 403 385 367 346 93 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2256 513 Size: 552 1191 4084 Fragments arranged by size: 4084 1191 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5088 Fragments arranged by size: 5088 683 56 BtsI GCAGTG_nn' Cuts at: 3108 3343 4137 4157 3108 Size: 235 794 20 4778 Fragments arranged by size: 4778 794 235 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5827 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5792 DraI TTT'AAA Cuts at: 3131 3131 Size: 5827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4834 809 Size: 507 3518 1802 Fragments arranged by size: 3518 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2334 2409 2583 Size: 54 1425 50 275 18 504 75 174 Cuts at: 2583 3274 4163 5792 8 Size: 691 889 1629 43 Fragments arranged by size: 1629 1425 889 691 504 275 174 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3274 1537 Size: 275 1462 4090 Fragments arranged by size: 4090 1462 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2145 3757 1353 Size: 792 1612 3423 Fragments arranged by size: 3423 1612 792 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4414 4788 4934 387 Size: 1064 138 2825 374 146 1280 Fragments arranged by size: 2825 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2132 2132 Size: 5827 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2238 2350 2717 3890 4487 5274 5542 Size: 393 606 112 367 1173 597 787 268 Cuts at: 5542 1239 Size: 1524 Fragments arranged by size: 1524 1173 787 606 597 393 367 268 112 EcoO109I rG'GnC_Cy Cuts at: 2494 2611 2494 Size: 117 5710 EcoRI G'AATT_C Cuts at: 3136 3514 3136 Size: 378 5449 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2122 2122 Size: 5827 FspI TGC'GCA Cuts at: 4333 4333 Size: 5827 HaeII r_GCGC'y Cuts at: 1678 4974 1678 Size: 3296 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2443 2475 2753 2785 4468 Size: 32 135 32 871 32 278 32 1683 Cuts at: 4468 4500 4542 4574 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1683 871 278 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3171 3417 669 Size: 988 281 1233 246 3079 Fragments arranged by size: 3079 1233 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5827 HpaI GTT'AAC Cuts at: 3417 3417 Size: 5827 KpnI G_GTAC'C Cuts at: 2266 3152 2266 Size: 886 4941 MfeI C'AATT_G Cuts at: 3426 3426 Size: 5827 MluI A'CGCG_T Cuts at: 5703 5703 Size: 5827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2803 4941 5125 1305 Size: 1498 2138 184 2007 Fragments arranged by size: 2138 2007 1498 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2411 2585 10 Size: 54 1425 343 579 174 3252 Fragments arranged by size: 3252 1425 579 343 174 54 MslI CAynn'nnrTG Cuts at: 518 1166 2238 2466 2628 2924 3785 4144 4303 Size: 648 1072 228 162 296 861 359 159 Cuts at: 4303 518 Size: 2042 Fragments arranged by size: 2042 1072 861 648 359 296 228 162 159 MspA1I CmG'CkG Cuts at: 1606 2027 3243 3920 5068 5313 1606 Size: 421 1216 677 1148 245 2120 Fragments arranged by size: 2120 1216 1148 677 421 245 NcoI C'CATG_G Cuts at: 513 1065 2256 513 Size: 552 1191 4084 Fragments arranged by size: 4084 1191 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5827 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5827 NotI GC'GGCC_GC Cuts at: 3274 3274 Size: 5827 NsiI A_TGCA'T Cuts at: 2878 2878 Size: 5827 NspI r_CATG'y Cuts at: 1132 1229 2536 2646 3185 4730 1132 Size: 97 1307 110 539 1545 2229 Fragments arranged by size: 2229 1545 1307 539 110 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5827 PciI A'CATG_T Cuts at: 2532 2642 4726 2532 Size: 110 2084 3633 Fragments arranged by size: 3633 2084 110 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5827 PmeI GTTT'AAAC Cuts at: 3131 3131 Size: 5827 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 5409 5441 3896 Size: 32 1481 32 4282 Fragments arranged by size: 4282 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2494 2494 Size: 5827 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5827 PsiI TTA'TAA Cuts at: 3397 3397 Size: 5827 PstI C_TGCA'G Cuts at: 830 3179 830 Size: 2349 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5827 PvuII CAG'CTG Cuts at: 2027 2027 Size: 5827 SacI G_AGCT'C Cuts at: 2134 2134 Size: 5827 SalI G'TCGA_C Cuts at: 1936 3169 1936 Size: 1233 4594 SbfI CC_TGCA'GG Cuts at: 3179 3179 Size: 5827 ScaI AGT'ACT Cuts at: 2284 4075 5787 2284 Size: 1791 1712 2324 Fragments arranged by size: 2324 1791 1712 SfcI C'TryA_G Cuts at: 826 1045 3175 4310 4991 5182 826 Size: 219 2130 1135 681 191 1471 Fragments arranged by size: 2130 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5827 SmaI CCC'GGG Cuts at: 1872 3154 1872 Size: 1282 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2278 3931 4832 5094 820 Size: 197 926 335 1653 901 262 1553 Fragments arranged by size: 1653 1553 926 901 335 262 197 SnaBI TAC'GTA Cuts at: 493 3618 493 Size: 3125 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5827 SphI G_CATG'C Cuts at: 1132 3185 1132 Size: 2053 3774 SspI AAT'ATT Cuts at: 5 52 2708 2959 3751 5 Size: 47 2656 251 792 2081 Fragments arranged by size: 2656 2081 792 251 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5827 StyI C'CwwG_G Cuts at: 513 1065 2169 2256 2634 2799 3266 513 Size: 552 1104 87 378 165 467 3074 Fragments arranged by size: 3074 1104 552 467 378 165 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3854 3871 4024 4209 4654 325 Size: 1255 2274 17 153 185 445 1498 Fragments arranged by size: 2274 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2282 2535 4073 Size: 274 80 33 51 1443 305 253 1538 Cuts at: 4073 5785 96 Size: 1712 138 Fragments arranged by size: 1712 1538 1443 305 274 253 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5787 5819 5787 Size: 32 5795 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5827 XbaI T'CTAG_A Cuts at: 3163 3163 Size: 5827 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2308 2308 Size: 5827 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5827 XmaI C'CCGG_G Cuts at: 1870 3152 1870 Size: 1282 4545 XmnI GAAnn'nnTTC Cuts at: 3956 3956 Size: 5827 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3635 276 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI