Restriction map for pFN21AB8979
 (Circular) (Six-base) MAPSORT of: pFN21AB8979.seq  Check: 3496  from: 1  to: 5827



pFN21AB8979  5827 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2744    2744 
   Size:       5827
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3637     278 
   Size:         53      83     186     936    2101    2468

  Fragments arranged by size: 

                2468    2101     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3170    1937 
   Size:       1233    4594
 
Acc65I G'GTAC_C

Cuts at:   2262    3148    2262 
   Size:        886    4941
 
AclI AA'CG_TT

Cuts at:   1563    3954    4327    1563 
   Size:       2391     373    3063

  Fragments arranged by size: 

                3063    2391     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2238    2350    2717    3890    5274    5542    1632 
   Size:        606     112     367    1173    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1173     606     367     268     112
 
AflII C'TTAA_G

Cuts at:    820    1017    2278     820 
   Size:        197    1261    4369

  Fragments arranged by size: 

                4369    1261     197
 
AflIII A'CryG_T

Cuts at:   1203    2532    2642    4726    5703    1203 
   Size:       1329     110    2084     977    1327

  Fragments arranged by size: 

                2084    1329    1327     977     110
 
AgeI A'CCGG_T

Cuts at:   4658    4658 
   Size:       5827
 
AhdI GACnn_n'nnGTC

Cuts at:   4556    4556 
   Size:       5827
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5827
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2646    1184 
   Size:       1462    4365
 
AlwNI CAG_nnn'CTG

Cuts at:   2030    3570    5142    2030 
   Size:       1540    1572    2715

  Fragments arranged by size: 

                2715    1572    1540
 
ApaLI G'TGCA_C

Cuts at:   3884    5040    3884 
   Size:       1156    4671
 
ApoI r'AATT_y

Cuts at:   1493    2140    3136    3362    3514    5623    1493 
   Size:        647     996     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     996     647     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4381     160 
   Size:        872    3349    1606

  Fragments arranged by size: 

                3349    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5827
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3152    1870 
   Size:         73    1209    4545

  Fragments arranged by size: 

                4545    1209      73
 
BamHI G'GATC_C

Cuts at:   3157    3524    3157 
   Size:        367    5460
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2262    3148    4603     618 
   Size:        325     217     627     475     886    1455    1842

  Fragments arranged by size: 

                1842    1455     886     627     475     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2134    3146    1391 
   Size:        743    1012    4072

  Fragments arranged by size: 

                4072    1012     743
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4856
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4018    4052    1903 
   Size:         34    2081      34    3678

  Fragments arranged by size: 

                3678    2081      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3719    4929    3719 
   Size:       1210    4617
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5827
 
BfrBI ATG'CAT

Cuts at:   2876    2876 
   Size:       5827
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4438     136 
   Size:        107     122      71     631     605    2766    1525

  Fragments arranged by size: 

                2766    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5822    5822 
   Size:       5827
 
BlpI GC'TnA_GC

Cuts at:   3244    3244 
   Size:       5827
 
Bme1580I G_kGCm'C

Cuts at:   1790    2216    3888    5044    1790 
   Size:        426    1672    1156    2573

  Fragments arranged by size: 

                2573    1672    1156     426
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5827
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2488    3558    4516 
   Size:        416      53      83     399      24    1071    1070     958

Cuts at:   4516     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1071    1070     958     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5827
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4487    1239 
   Size:       3248    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3952    4817    5115    3952 
   Size:        865     298    4664

  Fragments arranged by size: 

                4664     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4490     882 
   Size:        617    2991    2219

  Fragments arranged by size: 

                2991    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3618     493 
   Size:        711    2414    2702

  Fragments arranged by size: 

                2702    2414     711
 
BsaBI GATnn'nnATC

Cuts at:   5821    5821 
   Size:       5827
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3634    4016    4651     275 
   Size:         53      83     186     936    2101     382     635    1451

  Fragments arranged by size: 

                2101    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2862    4260    4658    4932    5079    5490    2862 
   Size:       1398     398     274     147     411    3199

  Fragments arranged by size: 

                3199    1398     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2780    2810    1178 
   Size:         30     165      30    1377      30    4195

  Fragments arranged by size: 

                4195    1377     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2906    1186 
   Size:       1720    4107
 
BseYI C'CCAG_C

Cuts at:   5030    5526    5030 
   Size:        496    5331
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5254
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3277    4038    5066    1540 
   Size:        275     186    1276     761    1028    2301

  Fragments arranged by size: 

                2301    1276    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2134    3888    3973    5044    2134 
   Size:       1754      85    1071    2917

  Fragments arranged by size: 

                2917    1754    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2804    3338    3431    2804 
   Size:        534      93    5200

  Fragments arranged by size: 

                5200     534      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2134    2216    3146    3888    3973    5044    1391 
   Size:        399     344      82     930     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     930     742     399     344      85      82
            
 
BspEI T'CCGG_A

Cuts at:   2862    5490    2862 
   Size:       2628    3199
 
BspHI T'CATG_A

Cuts at:   3716    5446    3716 
   Size:       1730    4097
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2305    2744    3182     844 
   Size:       1461     439     438    3489

  Fragments arranged by size: 

                3489    1461     439     438
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3714    1394 
   Size:        429    1891    3507

  Fragments arranged by size: 

                3507    1891     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2412    2438    4322    4496      65 
   Size:       1183    1164      26    1884     174    1396

  Fragments arranged by size: 

                1884    1396    1183    1164     174      26
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4471    4658    1700 
   Size:        110    2661     187    2869

  Fragments arranged by size: 

                2869    2661     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5827
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5827
 
BssSI C'ACGA_G

Cuts at:   3887    4899    3887 
   Size:       1012    4815
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5827
 
BstBI TT'CG_AA

Cuts at:   4631    4631 
   Size:       5827
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5827
 
BstYI r'GATC_y

Cuts at:   1972    2375    3064    3157    3524    3909    3926    5378    5464 
   Size:        403     689      93     367     385      17    1452      86

Cuts at:   5464    5476    5822    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     689     403     385     367     346      93
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2256     513 
   Size:        552    1191    4084

  Fragments arranged by size: 

                4084    1191     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5088

  Fragments arranged by size: 

                5088     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3108    3343    4137    4157    3108 
   Size:        235     794      20    4778

  Fragments arranged by size: 

                4778     794     235      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5827
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5792
 
DraI TTT'AAA

Cuts at:   3131    3131 
   Size:       5827
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4834     809 
   Size:        507    3518    1802

  Fragments arranged by size: 

                3518    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2334    2409    2583 
   Size:         54    1425      50     275      18     504      75     174

Cuts at:   2583    3274    4163    5792       8 
   Size:        691     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     691     504     275     174      75
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3274    1537 
   Size:        275    1462    4090

  Fragments arranged by size: 

                4090    1462     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2145    3757    1353 
   Size:        792    1612    3423

  Fragments arranged by size: 

                3423    1612     792
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4414    4788    4934     387 
   Size:       1064     138    2825     374     146    1280

  Fragments arranged by size: 

                2825    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2132    2132 
   Size:       5827
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2238    2350    2717    3890    4487    5274    5542 
   Size:        393     606     112     367    1173     597     787     268

Cuts at:   5542    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1173     787     606     597     393     367     268
                 112
 
EcoO109I rG'GnC_Cy

Cuts at:   2494    2611    2494 
   Size:        117    5710
 
EcoRI G'AATT_C

Cuts at:   3136    3514    3136 
   Size:        378    5449
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2122    2122 
   Size:       5827
 
FspI TGC'GCA

Cuts at:   4333    4333 
   Size:       5827
 
HaeII r_GCGC'y

Cuts at:   1678    4974    1678 
   Size:       3296    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2443    2475    2753    2785    4468 
   Size:         32     135      32     871      32     278      32    1683

Cuts at:   4468    4500    4542    4574    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1683     871     278     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3171    3417     669 
   Size:        988     281    1233     246    3079

  Fragments arranged by size: 

                3079    1233     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5827
 
HpaI GTT'AAC

Cuts at:   3417    3417 
   Size:       5827
 
KpnI G_GTAC'C

Cuts at:   2266    3152    2266 
   Size:        886    4941
 
MfeI C'AATT_G

Cuts at:   3426    3426 
   Size:       5827
 
MluI A'CGCG_T

Cuts at:   5703    5703 
   Size:       5827
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2803    4941    5125    1305 
   Size:       1498    2138     184    2007

  Fragments arranged by size: 

                2138    2007    1498     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2411    2585      10 
   Size:         54    1425     343     579     174    3252

  Fragments arranged by size: 

                3252    1425     579     343     174      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2238    2466    2628    2924    3785    4144    4303 
   Size:        648    1072     228     162     296     861     359     159

Cuts at:   4303     518 
   Size:       2042

  Fragments arranged by size: 

                2042    1072     861     648     359     296     228     162
                 159
 
MspA1I CmG'CkG

Cuts at:   1606    2027    3243    3920    5068    5313    1606 
   Size:        421    1216     677    1148     245    2120

  Fragments arranged by size: 

                2120    1216    1148     677     421     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2256     513 
   Size:        552    1191    4084

  Fragments arranged by size: 

                4084    1191     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5827
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5827
 
NotI GC'GGCC_GC

Cuts at:   3274    3274 
   Size:       5827
 
NsiI A_TGCA'T

Cuts at:   2878    2878 
   Size:       5827
 
NspI r_CATG'y

Cuts at:   1132    1229    2536    2646    3185    4730    1132 
   Size:         97    1307     110     539    1545    2229

  Fragments arranged by size: 

                2229    1545    1307     539     110      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5827
 
PciI A'CATG_T

Cuts at:   2532    2642    4726    2532 
   Size:        110    2084    3633

  Fragments arranged by size: 

                3633    2084     110
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5827
 
PmeI GTTT'AAAC

Cuts at:   3131    3131 
   Size:       5827
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3896    3928    5409    5441    3896 
   Size:         32    1481      32    4282

  Fragments arranged by size: 

                4282    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2494    2494 
   Size:       5827
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5827
 
PsiI TTA'TAA

Cuts at:   3397    3397 
   Size:       5827
 
PstI C_TGCA'G

Cuts at:    830    3179     830 
   Size:       2349    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5827
 
PvuII CAG'CTG

Cuts at:   2027    2027 
   Size:       5827
 
SacI G_AGCT'C

Cuts at:   2134    2134 
   Size:       5827
 
SalI G'TCGA_C

Cuts at:   1936    3169    1936 
   Size:       1233    4594
 
SbfI CC_TGCA'GG

Cuts at:   3179    3179 
   Size:       5827
 
ScaI AGT'ACT

Cuts at:   2284    4075    5787    2284 
   Size:       1791    1712    2324

  Fragments arranged by size: 

                2324    1791    1712
 
SfcI C'TryA_G

Cuts at:    826    1045    3175    4310    4991    5182     826 
   Size:        219    2130    1135     681     191    1471

  Fragments arranged by size: 

                2130    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5827
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5827
 
SmaI CCC'GGG

Cuts at:   1872    3154    1872 
   Size:       1282    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2278    3931    4832    5094     820 
   Size:        197     926     335    1653     901     262    1553

  Fragments arranged by size: 

                1653    1553     926     901     335     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3618     493 
   Size:       3125    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5827
 
SphI G_CATG'C

Cuts at:   1132    3185    1132 
   Size:       2053    3774
 
SspI AAT'ATT

Cuts at:      5      52    2708    2959    3751       5 
   Size:         47    2656     251     792    2081

  Fragments arranged by size: 

                2656    2081     792     251      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5827
 
StyI C'CwwG_G

Cuts at:    513    1065    2169    2256    2634    2799    3266     513 
   Size:        552    1104      87     378     165     467    3074

  Fragments arranged by size: 

                3074    1104     552     467     378     165      87
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3854    3871    4024    4209    4654     325 
   Size:       1255    2274      17     153     185     445    1498

  Fragments arranged by size: 

                2274    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2282    2535    4073 
   Size:        274      80      33      51    1443     305     253    1538

Cuts at:   4073    5785      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1538    1443     305     274     253     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5787    5819    5787 
   Size:         32    5795
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5827
 
XbaI T'CTAG_A

Cuts at:   3163    3163 
   Size:       5827
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2308    2308 
   Size:       5827
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5827
 
XmaI C'CCGG_G

Cuts at:   1870    3152    1870 
   Size:       1282    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3956    3956 
   Size:       5827
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3635     276 
   Size:         53      83     186     936    2101    2468

  Fragments arranged by size: 

                2468    2101     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI
     PstI     PvuI    PvuII     SacI     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
    EcoNI    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     PacI     PfoI     PmlI   PspOMI    PspXI     PsrI    RsrII
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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