(Circular) (Six-base) MAPSORT of: pFN21AB8938.seq Check: 537 from: 1 to: 5575 pFN21AB8938 5575 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3385 278 Size: 53 83 186 936 1849 2468 Fragments arranged by size: 2468 1849 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2593 2918 1937 Size: 656 325 4594 Fragments arranged by size: 4594 656 325 Acc65I G'GTAC_C Cuts at: 2896 2896 Size: 5575 AclI AA'CG_TT Cuts at: 1563 3702 4075 1563 Size: 2139 373 3063 Fragments arranged by size: 3063 2139 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3638 5022 5290 1632 Size: 2006 1384 268 1917 Fragments arranged by size: 2006 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5378 AflIII A'CryG_T Cuts at: 1203 4474 5451 1203 Size: 3271 977 1327 Fragments arranged by size: 3271 1327 977 AgeI A'CCGG_T Cuts at: 4406 4406 Size: 5575 AhdI GACnn_n'nnGTC Cuts at: 4304 4304 Size: 5575 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5575 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5575 AlwNI CAG_nnn'CTG Cuts at: 2588 3318 4890 2588 Size: 730 1572 3273 Fragments arranged by size: 3273 1572 730 ApaLI G'TGCA_C Cuts at: 3632 4788 3632 Size: 1156 4419 ApoI r'AATT_y Cuts at: 1493 2648 2783 2884 3110 3262 5371 1493 Size: 1155 135 101 226 152 2109 1697 Fragments arranged by size: 2109 1697 1155 226 152 135 101 AseI AT'TA_AT Cuts at: 160 1032 4129 160 Size: 872 3097 1606 Fragments arranged by size: 3097 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 AvaI C'yCGr_G Cuts at: 1870 1943 2117 2900 1870 Size: 73 174 783 4545 Fragments arranged by size: 4545 783 174 73 BamHI G'GATC_C Cuts at: 2905 3272 2905 Size: 367 5208 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2896 4351 618 Size: 325 217 627 1109 1455 1842 Fragments arranged by size: 1842 1455 1109 627 325 217 BanII G_rGCy'C Cuts at: 1391 2403 2543 2894 1391 Size: 1012 140 351 4072 Fragments arranged by size: 4072 1012 351 140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2611 2856 928 Size: 971 712 245 3647 Fragments arranged by size: 3647 971 712 245 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3766 3800 1903 Size: 34 1829 34 3678 Fragments arranged by size: 3678 1829 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2411 3467 4677 2411 Size: 1056 1210 3309 Fragments arranged by size: 3309 1210 1056 BclI T'GATC_A Cuts at: 1548 1548 Size: 5575 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2321 4186 136 Size: 107 122 71 631 605 649 1865 1525 Fragments arranged by size: 1865 1525 649 631 605 122 107 71 BglII A'GATC_T Cuts at: 5570 5570 Size: 5575 BlpI GC'TnA_GC Cuts at: 2992 2992 Size: 5575 Bme1580I G_kGCm'C Cuts at: 1790 3636 4792 1790 Size: 1846 1156 2573 Fragments arranged by size: 2573 1846 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5575 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2256 3306 4264 Size: 416 53 83 399 24 839 1050 958 Cuts at: 4264 442 Size: 1753 Fragments arranged by size: 1753 1050 958 839 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5575 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2562 2562 Size: 5575 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2864 4235 1239 Size: 1625 1371 2579 Fragments arranged by size: 2579 1625 1371 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3700 4565 4863 3700 Size: 865 298 4412 Fragments arranged by size: 4412 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2566 4238 882 Size: 617 1067 1672 2219 Fragments arranged by size: 2219 1672 1067 617 BsaAI yAC'GTr Cuts at: 493 1204 3366 493 Size: 711 2162 2702 Fragments arranged by size: 2702 2162 711 BsaBI GATnn'nnATC Cuts at: 5569 5569 Size: 5575 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3382 3764 4399 275 Size: 53 83 186 936 1849 382 635 1451 Fragments arranged by size: 1849 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4008 4406 4680 4827 5238 4008 Size: 398 274 147 411 4345 Fragments arranged by size: 4345 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5350 Fragments arranged by size: 5350 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2325 2328 1186 Size: 1139 3 4433 Fragments arranged by size: 4433 1139 3 BseYI C'CCAG_C Cuts at: 2454 4778 5274 2454 Size: 2324 496 2755 Fragments arranged by size: 2755 2324 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5002 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2131 2310 3025 3786 4814 1540 Size: 275 186 130 179 715 761 1028 2301 Fragments arranged by size: 2301 1028 761 715 275 186 179 130 BsiHKAI G_wGCw'C Cuts at: 2543 3636 3721 4792 2543 Size: 1093 85 1071 3326 Fragments arranged by size: 3326 1093 1071 85 BsmI GAATG_Cn' Cuts at: 3086 3179 3086 Size: 93 5482 BsmBI CGTCTCn'nnnn_ Cuts at: 2550 2550 Size: 5575 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2403 2543 2894 3636 3721 4792 1391 Size: 399 613 140 351 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 613 399 351 140 85 BspEI T'CCGG_A Cuts at: 5238 5238 Size: 5575 BspHI T'CATG_A Cuts at: 3464 5194 3464 Size: 1730 3845 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2930 844 Size: 2086 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3462 1394 Size: 429 1639 3507 Fragments arranged by size: 3507 1639 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4070 4244 65 Size: 1183 2822 174 1396 Fragments arranged by size: 2822 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2180 4219 4406 1700 Size: 110 370 2039 187 2869 Fragments arranged by size: 2869 2039 370 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5575 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5575 BssSI C'ACGA_G Cuts at: 3635 4647 3635 Size: 1012 4563 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5575 BstBI TT'CG_AA Cuts at: 4379 4379 Size: 5575 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5575 BstYI r'GATC_y Cuts at: 1972 2905 3272 3657 3674 5126 5212 5224 5570 Size: 933 367 385 17 1452 86 12 346 Cuts at: 5570 1972 Size: 1977 Fragments arranged by size: 1977 1452 933 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2564 513 Size: 552 1499 3524 Fragments arranged by size: 3524 1499 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4836 Fragments arranged by size: 4836 683 56 BtsI GCAGTG_nn' Cuts at: 3091 3885 3905 3091 Size: 794 20 4761 Fragments arranged by size: 4761 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5575 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5540 DraI TTT'AAA Cuts at: 2879 2879 Size: 5575 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4582 809 Size: 507 3266 1802 Fragments arranged by size: 3266 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2128 2307 3022 Size: 54 1425 50 275 18 298 179 715 Cuts at: 3022 3911 5540 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 715 298 275 179 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2128 2307 3022 1537 Size: 275 316 179 715 4090 Fragments arranged by size: 4090 715 316 275 179 EarI CTCTTCn'nnn_ Cuts at: 1353 2593 3505 1353 Size: 1240 912 3423 Fragments arranged by size: 3423 1240 912 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2340 2346 4162 4536 4682 387 Size: 1064 138 751 6 1816 374 146 1280 Fragments arranged by size: 1816 1280 1064 751 374 146 138 6 EcoICRI GAG'CTC Cuts at: 2541 2541 Size: 5575 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2864 3638 4235 5022 5290 1239 Size: 393 1232 774 597 787 268 1524 Fragments arranged by size: 1524 1232 787 774 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2518 2518 Size: 5575 EcoRI G'AATT_C Cuts at: 2884 3262 2884 Size: 378 5197 FspI TGC'GCA Cuts at: 4081 4081 Size: 5575 HaeII r_GCGC'y Cuts at: 1678 2178 4722 1678 Size: 500 2544 2531 Fragments arranged by size: 2544 2531 500 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2374 2406 2477 2509 4216 Size: 32 135 32 802 32 71 32 1707 Cuts at: 4216 4248 4290 4322 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1707 802 135 71 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2919 3165 669 Size: 988 281 981 246 3079 Fragments arranged by size: 3079 988 981 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5575 HpaI GTT'AAC Cuts at: 3165 3165 Size: 5575 KpnI G_GTAC'C Cuts at: 2900 2900 Size: 5575 MfeI C'AATT_G Cuts at: 3174 3174 Size: 5575 MluI A'CGCG_T Cuts at: 5451 5451 Size: 5575 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2619 4689 4873 1305 Size: 1314 2070 184 2007 Fragments arranged by size: 2070 2007 1314 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3753 Fragments arranged by size: 3753 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3533 3892 4051 518 Size: 648 2367 359 159 2042 Fragments arranged by size: 2367 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2312 2991 3668 4816 5061 1606 Size: 423 283 679 677 1148 245 2120 Fragments arranged by size: 2120 1148 679 677 423 283 245 NaeI GCC'GGC Cuts at: 2182 2182 Size: 5575 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5023 NdeI CA'TA_TG Cuts at: 387 387 Size: 5575 NgoMIV G'CCGG_C Cuts at: 2180 2180 Size: 5575 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5575 NotI GC'GGCC_GC Cuts at: 3022 3022 Size: 5575 NspI r_CATG'y Cuts at: 1132 1229 2933 4478 1132 Size: 97 1704 1545 2229 Fragments arranged by size: 2229 1704 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5575 PciI A'CATG_T Cuts at: 4474 4474 Size: 5575 PflMI CCAn_nnn'nTGG Cuts at: 1108 2842 1108 Size: 1734 3841 PfoI T'CCnGG_A Cuts at: 2240 2240 Size: 5575 PmeI GTTT'AAAC Cuts at: 2879 2879 Size: 5575 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3644 3676 5157 5189 3644 Size: 32 1481 32 4030 Fragments arranged by size: 4030 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2518 2518 Size: 5575 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5575 PsiI TTA'TAA Cuts at: 3145 3145 Size: 5575 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2015 2047 2015 Size: 32 5543 PstI C_TGCA'G Cuts at: 830 2160 2927 830 Size: 1330 767 3478 Fragments arranged by size: 3478 1330 767 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5575 SacI G_AGCT'C Cuts at: 2543 2543 Size: 5575 SalI G'TCGA_C Cuts at: 1936 2917 1936 Size: 981 4594 SbfI CC_TGCA'GG Cuts at: 2927 2927 Size: 5575 ScaI AGT'ACT Cuts at: 2282 3823 5535 2282 Size: 1541 1712 2322 Fragments arranged by size: 2322 1712 1541 SfcI C'TryA_G Cuts at: 826 1045 2156 2614 2923 4058 4739 4930 826 Size: 219 1111 458 309 1135 681 191 1471 Fragments arranged by size: 1471 1135 1111 681 458 309 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5575 SmaI CCC'GGG Cuts at: 1872 2902 1872 Size: 1030 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3679 4580 4842 820 Size: 197 926 1736 901 262 1553 Fragments arranged by size: 1736 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3366 493 Size: 2873 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5575 SphI G_CATG'C Cuts at: 1132 2933 1132 Size: 1801 3774 SspI AAT'ATT Cuts at: 5 52 2509 3499 5 Size: 47 2457 990 2081 Fragments arranged by size: 2457 2081 990 47 StuI AGG'CCT Cuts at: 1517 2865 1517 Size: 1348 4227 StyI C'CwwG_G Cuts at: 513 1065 3014 513 Size: 552 1949 3074 Fragments arranged by size: 3074 1949 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2238 3602 3619 3772 3957 4402 325 Size: 1255 658 1364 17 153 185 445 1498 Fragments arranged by size: 1498 1364 1255 658 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2031 2040 2280 Size: 274 80 33 51 1443 54 9 240 Cuts at: 2280 3821 5533 96 Size: 1541 1712 138 Fragments arranged by size: 1712 1541 1443 274 240 138 80 54 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5535 5567 5535 Size: 32 5543 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5575 XbaI T'CTAG_A Cuts at: 2632 2911 2632 Size: 279 5296 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5575 XmaI C'CCGG_G Cuts at: 1870 2900 1870 Size: 1030 4545 XmnI GAAnn'nnTTC Cuts at: 3704 3704 Size: 5575 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3383 276 Size: 53 83 186 936 1849 2468 Fragments arranged by size: 2468 1849 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI