(Circular) (Six-base) MAPSORT of: pFN21AB8932.seq Check: 4198 from: 1 to: 5554 pFN21AB8932 5554 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3364 278 Size: 53 83 186 936 1828 2468 Fragments arranged by size: 2468 1828 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2375 2897 1937 Size: 438 522 4594 Fragments arranged by size: 4594 522 438 Acc65I G'GTAC_C Cuts at: 2875 2875 Size: 5554 AclI AA'CG_TT Cuts at: 1563 3681 4054 1563 Size: 2118 373 3063 Fragments arranged by size: 3063 2118 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3617 5001 5269 1632 Size: 1985 1384 268 1917 Fragments arranged by size: 1985 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5357 AflIII A'CryG_T Cuts at: 1203 2388 4453 5430 1203 Size: 1185 2065 977 1327 Fragments arranged by size: 2065 1327 1185 977 AgeI A'CCGG_T Cuts at: 4385 4385 Size: 5554 AhdI GACnn_n'nnGTC Cuts at: 4283 4283 Size: 5554 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5554 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5554 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2480 2512 2480 Size: 32 5522 AlwNI CAG_nnn'CTG Cuts at: 3297 4869 3297 Size: 1572 3982 ApaI G_GGCC'C Cuts at: 2301 2301 Size: 5554 ApaLI G'TGCA_C Cuts at: 3611 4767 3611 Size: 1156 4398 ApoI r'AATT_y Cuts at: 1493 2863 3089 3241 5350 1493 Size: 1370 226 152 2109 1697 Fragments arranged by size: 2109 1697 1370 226 152 AseI AT'TA_AT Cuts at: 160 1032 4108 160 Size: 872 3076 1606 Fragments arranged by size: 3076 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 AvaI C'yCGr_G Cuts at: 1870 1943 2772 2879 1870 Size: 73 829 107 4545 Fragments arranged by size: 4545 829 107 73 BamHI G'GATC_C Cuts at: 2884 3251 2884 Size: 367 5187 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2077 2159 2212 2414 2875 Size: 325 217 627 290 82 53 202 461 Cuts at: 2875 4330 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 461 325 290 217 202 82 53 BanII G_rGCy'C Cuts at: 1391 2301 2340 2873 1391 Size: 910 39 533 4072 Fragments arranged by size: 4072 910 533 39 BbeI G_GCGC'C Cuts at: 2081 2163 2418 2081 Size: 82 255 5217 Fragments arranged by size: 5217 255 82 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4583 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3745 3779 1903 Size: 34 1808 34 3678 Fragments arranged by size: 3678 1808 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3446 4656 3446 Size: 1210 4344 BclI T'GATC_A Cuts at: 1548 1548 Size: 5554 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2296 2836 4165 Size: 107 122 71 631 605 624 540 1329 Cuts at: 4165 136 Size: 1525 Fragments arranged by size: 1525 1329 631 624 605 540 122 107 71 BglII A'GATC_T Cuts at: 2577 5549 2577 Size: 2972 2582 BlpI GC'TnA_GC Cuts at: 2268 2971 2268 Size: 703 4851 Bme1580I G_kGCm'C Cuts at: 1790 2217 2301 3615 4771 1790 Size: 427 84 1314 1156 2573 Fragments arranged by size: 2573 1314 1156 427 84 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5554 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3285 4243 442 Size: 416 53 83 399 24 1868 958 1753 Fragments arranged by size: 1868 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5554 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2195 2351 2507 2803 4214 1239 Size: 956 156 156 296 1411 2579 Fragments arranged by size: 2579 1411 956 296 156 156 Bpu10I CC'TnA_GC Cuts at: 2673 2798 2673 Size: 125 5429 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3679 4544 4842 3679 Size: 865 298 4391 Fragments arranged by size: 4391 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4217 882 Size: 617 2718 2219 Fragments arranged by size: 2718 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3345 493 Size: 711 2141 2702 Fragments arranged by size: 2702 2141 711 BsaBI GATnn'nnATC Cuts at: 5548 5548 Size: 5554 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2078 2160 2325 2415 Size: 53 83 186 936 545 82 165 90 Cuts at: 2415 3361 3743 4378 275 Size: 946 382 635 1451 Fragments arranged by size: 1451 946 936 635 545 382 186 165 90 83 82 53 BsaWI w'CCGG_w Cuts at: 3987 4385 4659 4806 5217 3987 Size: 398 274 147 411 4324 Fragments arranged by size: 4324 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2480 2510 2730 2760 1178 Size: 30 165 30 1077 30 220 30 3972 Fragments arranged by size: 3972 1077 220 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2348 2450 2630 2717 1186 Size: 844 318 102 180 87 4023 Fragments arranged by size: 4023 844 318 180 102 87 BseYI C'CCAG_C Cuts at: 4757 5253 4757 Size: 496 5058 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4981 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2525 2840 3004 3765 4793 1540 Size: 275 186 524 315 164 761 1028 2301 Fragments arranged by size: 2301 1028 761 524 315 275 186 164 BsiHKAI G_wGCw'C Cuts at: 3615 3700 4771 3615 Size: 85 1071 4398 Fragments arranged by size: 4398 1071 85 BsiWI C'GTAC_G Cuts at: 2205 2205 Size: 5554 BsmI GAATG_Cn' Cuts at: 3065 3158 3065 Size: 93 5461 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2301 2340 2873 3615 3700 4771 Size: 399 427 84 39 533 742 85 1071 Cuts at: 4771 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 533 427 399 85 84 39 BspEI T'CCGG_A Cuts at: 5217 5217 Size: 5554 BspHI T'CATG_A Cuts at: 3443 5173 3443 Size: 1730 3824 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2909 844 Size: 2065 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3441 1394 Size: 429 1618 3507 Fragments arranged by size: 3507 1618 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4049 4223 65 Size: 1183 2801 174 1396 Fragments arranged by size: 2801 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4198 4385 1700 Size: 110 2388 187 2869 Fragments arranged by size: 2869 2388 187 110 BsrGI T'GTAC_A Cuts at: 96 2112 96 Size: 2016 3538 BssHII G'CGCG_C Cuts at: 1925 2169 2467 1925 Size: 244 298 5012 Fragments arranged by size: 5012 298 244 BssSI C'ACGA_G Cuts at: 2850 3614 4626 2850 Size: 764 1012 3778 Fragments arranged by size: 3778 1012 764 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5554 BstBI TT'CG_AA Cuts at: 4358 4358 Size: 5554 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5554 BstYI r'GATC_y Cuts at: 1972 2577 2884 3251 3636 3653 5105 5191 5203 Size: 605 307 367 385 17 1452 86 12 Cuts at: 5203 5549 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 605 385 367 346 307 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2235 2363 513 Size: 552 1170 128 3704 Fragments arranged by size: 3704 1170 552 128 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4815 Fragments arranged by size: 4815 683 56 BtsI GCAGTG_nn' Cuts at: 3070 3864 3884 3070 Size: 794 20 4740 Fragments arranged by size: 4740 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5554 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5519 DraI TTT'AAA Cuts at: 2858 2858 Size: 5554 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4561 809 Size: 507 3245 1802 Fragments arranged by size: 3245 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2522 2837 Size: 54 1425 50 275 18 201 491 315 Cuts at: 2837 3001 3890 5519 8 Size: 164 889 1629 43 Fragments arranged by size: 1629 1425 889 491 315 275 201 164 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2522 2837 3001 1537 Size: 275 710 315 164 4090 Fragments arranged by size: 4090 710 315 275 164 EarI CTCTTCn'nnn_ Cuts at: 1353 3484 1353 Size: 2131 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4141 4515 4661 387 Size: 1064 138 2552 374 146 1280 Fragments arranged by size: 2552 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2351 2507 2803 3617 4214 5001 Size: 393 563 156 156 296 814 597 787 Cuts at: 5001 5269 1239 Size: 268 1524 Fragments arranged by size: 1524 814 787 597 563 393 296 268 156 156 EcoO109I rG'GnC_Cy Cuts at: 2050 2298 2050 Size: 248 5306 EcoRI G'AATT_C Cuts at: 2863 3241 2863 Size: 378 5176 FspI TGC'GCA Cuts at: 4060 4060 Size: 5554 HaeII r_GCGC'y Cuts at: 1678 2081 2094 2163 2418 2717 4701 1678 Size: 403 13 69 255 299 1984 2531 Fragments arranged by size: 2531 1984 403 299 255 69 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4195 4227 4269 4301 1373 Size: 32 135 32 2623 32 42 32 2626 Fragments arranged by size: 2626 2623 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2898 3144 669 Size: 988 281 960 246 3079 Fragments arranged by size: 3079 988 960 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5554 HpaI GTT'AAC Cuts at: 3144 3144 Size: 5554 KasI G'GCGC_C Cuts at: 2077 2159 2414 2077 Size: 82 255 5217 Fragments arranged by size: 5217 255 82 KpnI G_GTAC'C Cuts at: 2879 2879 Size: 5554 MfeI C'AATT_G Cuts at: 3153 3153 Size: 5554 MluI A'CGCG_T Cuts at: 2388 5430 2388 Size: 3042 2512 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4668 4852 1305 Size: 3363 184 2007 Fragments arranged by size: 3363 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3732 Fragments arranged by size: 3732 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3512 3871 4030 518 Size: 648 2346 359 159 2042 Fragments arranged by size: 2346 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2237 2353 2476 2645 2756 2970 3647 4795 Size: 631 116 123 169 111 214 677 1148 Cuts at: 4795 5040 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 631 245 214 169 123 116 111 NarI GG'CG_CC Cuts at: 2078 2160 2415 2078 Size: 82 255 5217 Fragments arranged by size: 5217 255 82 NcoI C'CATG_G Cuts at: 513 1065 2363 513 Size: 552 1298 3704 Fragments arranged by size: 3704 1298 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5554 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5554 NotI GC'GGCC_GC Cuts at: 2837 3001 2837 Size: 164 5390 NspI r_CATG'y Cuts at: 1132 1229 2912 4457 1132 Size: 97 1683 1545 2229 Fragments arranged by size: 2229 1683 1545 97 PasI CC'CwG_GG Cuts at: 1348 2164 1348 Size: 816 4738 PciI A'CATG_T Cuts at: 4453 4453 Size: 5554 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5554 PfoI T'CCnGG_A Cuts at: 2304 2328 2304 Size: 24 5530 PmeI GTTT'AAAC Cuts at: 2858 2858 Size: 5554 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2480 2512 3623 3655 5136 5168 2480 Size: 32 1111 32 1481 32 2866 Fragments arranged by size: 2866 1481 1111 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2050 2050 Size: 5554 PshAI GACnn'nnGTC Cuts at: 1613 2645 1613 Size: 1032 4522 PsiI TTA'TAA Cuts at: 3124 3124 Size: 5554 PspOMI G'GGCC_C Cuts at: 2297 2297 Size: 5554 PstI C_TGCA'G Cuts at: 830 2906 830 Size: 2076 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5554 PvuII CAG'CTG Cuts at: 2353 2476 2645 2353 Size: 123 169 5262 Fragments arranged by size: 5262 169 123 SacII CC_GC'GG Cuts at: 2238 2238 Size: 5554 SalI G'TCGA_C Cuts at: 1936 2896 1936 Size: 960 4594 SbfI CC_TGCA'GG Cuts at: 2906 2906 Size: 5554 ScaI AGT'ACT Cuts at: 2024 3802 5514 2024 Size: 1778 1712 2064 Fragments arranged by size: 2064 1778 1712 SfcI C'TryA_G Cuts at: 826 1045 2902 4037 4718 4909 826 Size: 219 1857 1135 681 191 1471 Fragments arranged by size: 1857 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2079 2161 2416 2079 Size: 82 255 5217 Fragments arranged by size: 5217 255 82 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5554 SmaI CCC'GGG Cuts at: 1872 2881 1872 Size: 1009 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3658 4559 4821 820 Size: 197 926 1715 901 262 1553 Fragments arranged by size: 1715 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3345 493 Size: 2852 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5554 SphI G_CATG'C Cuts at: 1132 2912 1132 Size: 1780 3774 SspI AAT'ATT Cuts at: 5 52 3478 5 Size: 47 3426 2081 Fragments arranged by size: 3426 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5554 StyI C'CwwG_G Cuts at: 513 1065 2363 2993 513 Size: 552 1298 630 3074 Fragments arranged by size: 3074 1298 630 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2576 3581 3598 3751 3936 4381 325 Size: 1255 996 1005 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1005 996 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2022 2112 2511 Size: 274 80 33 51 1443 45 90 399 Cuts at: 2511 3800 5512 96 Size: 1289 1712 138 Fragments arranged by size: 1712 1443 1289 399 274 138 90 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2730 2762 5514 5546 2730 Size: 32 2752 32 2738 Fragments arranged by size: 2752 2738 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5554 XbaI T'CTAG_A Cuts at: 2890 2890 Size: 5554 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5554 XmaI C'CCGG_G Cuts at: 1870 2879 1870 Size: 1009 4545 XmnI GAAnn'nnTTC Cuts at: 2495 3683 2495 Size: 1188 4366 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3362 276 Size: 53 83 186 936 1828 2468 Fragments arranged by size: 2468 1828 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI