Restriction map for
pFN21AB8932
(Linear) (Six-base) MAPSORT of: KIBB8932.seq Check: 2394 from: 1 to: 849
KIBB8932.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 370 849
Size: 370 479
AflIII A'CryG_T
Cuts at: 0 383 849
Size: 383 466
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 475 507 849
Size: 475 32 342
Fragments arranged by size:
475 342 32
ApaI G_GGCC'C
Cuts at: 0 296 849
Size: 296 553
AvaI C'yCGr_G
Cuts at: 0 767 849
Size: 767 82
BanI G'GyrC_C
Cuts at: 0 72 154 207 409 849
Size: 72 82 53 202 440
Fragments arranged by size:
440 202 82 72 53
BanII G_rGCy'C
Cuts at: 0 296 335 849
Size: 296 39 514
Fragments arranged by size:
514 296 39
BbeI G_GCGC'C
Cuts at: 0 76 158 413 849
Size: 76 82 255 436
Fragments arranged by size:
436 255 82 76
BglI GCCn_nnn'nGGC
Cuts at: 0 291 831 849
Size: 291 540 18
Fragments arranged by size:
540 291 18
BglII A'GATC_T
Cuts at: 0 572 849
Size: 572 277
BlpI GC'TnA_GC
Cuts at: 0 263 849
Size: 263 586
Bme1580I G_kGCm'C
Cuts at: 0 212 296 849
Size: 212 84 553
Fragments arranged by size:
553 212 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 346 502 798 849
Size: 190 156 156 296 51
Fragments arranged by size:
296 190 156 156 51
Bpu10I CC'TnA_GC
Cuts at: 0 668 793 849
Size: 668 125 56
Fragments arranged by size:
668 125 56
BsaHI Gr'CG_yC
Cuts at: 0 73 155 320 410 849
Size: 73 82 165 90 439
Fragments arranged by size:
439 165 90 82 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 475 505 725 755 849
Size: 475 30 220 30 94
Fragments arranged by size:
475 220 94 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 343 445 625 712 849
Size: 25 318 102 180 87 137
Fragments arranged by size:
318 180 137 102 87 25
BsiEI CG_ry'CG
Cuts at: 0 520 835 849
Size: 520 315 14
Fragments arranged by size:
520 315 14
BsiWI C'GTAC_G
Cuts at: 0 200 849
Size: 200 649
Bsp1286I G_dGCh'C
Cuts at: 0 212 296 335 849
Size: 212 84 39 514
Fragments arranged by size:
514 212 84 39
BsrGI T'GTAC_A
Cuts at: 0 107 849
Size: 107 742
BssHII G'CGCG_C
Cuts at: 0 164 462 849
Size: 164 298 387
Fragments arranged by size:
387 298 164
BstYI r'GATC_y
Cuts at: 0 572 849
Size: 572 277
BtgI C'CryG_G
Cuts at: 0 230 358 849
Size: 230 128 491
Fragments arranged by size:
491 230 128
EaeI y'GGCC_r
Cuts at: 0 26 517 832 849
Size: 26 491 315 17
Fragments arranged by size:
491 315 26 17
EagI C'GGCC_G
Cuts at: 0 517 832 849
Size: 517 315 17
Fragments arranged by size:
517 315 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 346 502 798 849
Size: 190 156 156 296 51
Fragments arranged by size:
296 190 156 156 51
EcoO109I rG'GnC_Cy
Cuts at: 0 45 293 849
Size: 45 248 556
Fragments arranged by size:
556 248 45
HaeII r_GCGC'y
Cuts at: 0 76 89 158 413 712 849
Size: 76 13 69 255 299 137
Fragments arranged by size:
299 255 137 76 69 13
KasI G'GCGC_C
Cuts at: 0 72 154 409 849
Size: 72 82 255 440
Fragments arranged by size:
440 255 82 72
MluI A'CGCG_T
Cuts at: 0 383 849
Size: 383 466
MspA1I CmG'CkG
Cuts at: 0 232 348 471 640 751 849
Size: 232 116 123 169 111 98
Fragments arranged by size:
232 169 123 116 111 98
NarI GG'CG_CC
Cuts at: 0 73 155 410 849
Size: 73 82 255 439
Fragments arranged by size:
439 255 82 73
NcoI C'CATG_G
Cuts at: 0 358 849
Size: 358 491
NotI GC'GGCC_GC
Cuts at: 0 832 849
Size: 832 17
PasI CC'CwG_GG
Cuts at: 0 159 849
Size: 159 690
PfoI T'CCnGG_A
Cuts at: 0 299 323 849
Size: 299 24 526
Fragments arranged by size:
526 299 24
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 475 507 849
Size: 475 32 342
Fragments arranged by size:
475 342 32
PpuMI rG'GwC_Cy
Cuts at: 0 45 849
Size: 45 804
PshAI GACnn'nnGTC
Cuts at: 0 640 849
Size: 640 209
PspOMI G'GGCC_C
Cuts at: 0 292 849
Size: 292 557
PvuII CAG'CTG
Cuts at: 0 348 471 640 849
Size: 348 123 169 209
Fragments arranged by size:
348 209 169 123
SacII CC_GC'GG
Cuts at: 0 233 849
Size: 233 616
ScaI AGT'ACT
Cuts at: 0 19 849
Size: 19 830
SfoI GGC'GCC
Cuts at: 0 74 156 411 849
Size: 74 82 255 438
Fragments arranged by size:
438 255 82 74
StyI C'CwwG_G
Cuts at: 0 358 849
Size: 358 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 571 849
Size: 571 278
TatI w'GTAC_w
Cuts at: 0 17 107 506 849
Size: 17 90 399 343
Fragments arranged by size:
399 343 90 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 725 757 849
Size: 725 32 92
Fragments arranged by size:
725 92 32
XmnI GAAnn'nnTTC
Cuts at: 0 490 849
Size: 490 359
Enzymes that do cut:
AccI AflIII AloI ApaI AvaI BanI BanII BbeI
BglI BglII BlpI Bme1580I BpmI Bpu10I BsaHI BsaXI
BseRI BsiEI BsiWI Bsp1286I BsrGI BssHII BstYI BtgI
EaeI EagI Eco57MI EcoO109I HaeII KasI MluI MspA1I
NarI NcoI NotI PasI PfoI PpiI PpuMI PshAI
PspOMI PvuII SacII ScaI SfoI StyI TaqII TatI
TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BmgBI BmrI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaWI BseYI BsgI BsiHKAI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MmeI MscI MslI NaeI
NdeI NgoMIV NheI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PsiI PspXI PsrI PstI PvuI
RsrII SacI SalI SanDI SapI SbfI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI
XmaI ZraI