Restriction map for
pFN21AB8901
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaII AviII AvrII
BbeI BbrPI BbsI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HpaI KasI MaeII MamI
MfeI MluI MroI MseI MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 299 GGTACC G^GTACC
AccI 463 GTAGAC GT^[AC][GT]AC
AccII 189 CGCG CG^CG
AfaI 300 GTAC GT^AC
Aor51HI 264 AGCGCT AGC^GCT
Asp718I 299 GGTACC G^GTACC
AvaI 230 CCCGGG C^[CT]CG[GA]G
BamHI 542 GGATCC G^GATCC
BanII 678 GGGCTC G[GA]GC[CT]^C
BbvCI 267 GCTGAGG CCTCAGC(-5/-2)
BfaI 532 CTAG C^TAG
BglI 608 GCCGAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 69 CTGGAG CTGGAG(16/14)
BsaI 136 GAGACC GGTCTC(1/5)
BsmI 310 GCATTC GAATGC(1/-1)
BsoBI 230 CCCGGG C^[CT]CG[GA]G
BspMI 477 GCAGGT ACCTGC(4/8)
BstUI 189 CGCG CG^CG
BstYI 542 GGATCC [GA]^GATC[CT]
CfoI 265 GCGC GCG^C
DraII 107 AGGCCCC [GA]G^G[ACGT]CC[CT]
Eco47III 264 AGCGCT AGC^GCT
EcoO109I 107 AGGCCCC [GA]G^G[ACGT]CC[CT]
HaeII 264 AGCGCT [GA]GCGC^[CT]
HhaI 265 GCGC GCG^C
HinP1I 265 GCGC G^CGC
HincII 28 GTTGAC GT[CT]^[GA]AC
HindII 28 GTTGAC GT[CT]^[GA]AC
HindIII 46 AAGCTT A^AGCTT
KpnI 299 GGTACC GGTAC^C
KspI 188 CCGCGG CCGC^GG
MaeI 532 CTAG C^TAG
MaeIII 317 GTGAC ^GT[ACGT]AC
MflI 542 GGATCC [GA]^GATC[CT]
MslI 651 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 189 CGCG CG^CG
NaeI 661 GCCGGC GCC^GGC
NcoI 245 CCATGG C^CATGG
NgoMIV 661 GCCGGC G^CCGGC
PvuII 66 CAGCTG CAG^CTG
RsaI 300 GTAC GT^AC
SacII 188 CCGCGG CCGC^GG
SmaI 230 CCCGGG CCC^GGG
TfiI 124 GAATC G^A[AT]TC
Tsp45I 317 GTGAC ^GT[GC]AC
XhoII 542 GGATCC [GA]^GATC[CT]
XmaCI 230 CCCGGG C^CCGGG
XmaI 230 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BalI 207 TGGCCA TGG^CCA
509 TGGCCA
BanI 170 GGCACC G^G[CT][GA]CC
299 GGTACC
BciVI 61 GGATAC GTATCC(6/5)
564 GGATAC
BmyI 443 GGGCAC G[AGT]GC[ACT]^C
678 GGGCTC
BsmAI 136 GAGAC GTCTC(1/5)
3 GTCTC
BsmFI 118 GGGAC GGGAC(10/14)
349 GGGAC
Bsp1286I 443 GGGCAC G[AGT]GC[ACT]^C
678 GGGCTC
BsrFI 302 ACCGGC [GA]^CCGG[CT]
661 GCCGGC
Cfr10I 302 ACCGGC [GA]^CCGG[CT]
661 GCCGGC
Cfr13I 108 GGCCC G^G[ACGT]CC
615 GGGCC
DsaI 188 CCGCGG C^C[GA][CT]GG
245 CCATGG
EcoT14I 245 CCATGG C^C[AT][AT]GG
538 CCTTGG
MluNI 207 TGGCCA TGG^CCA
509 TGGCCA
MscI 207 TGGCCA TGG^CCA
509 TGGCCA
NlaIII 246 CATG CATG^
651 CATG
PleI 72 GAGTC GAGTC(4/5)
489 GAGTC
Sau96I 108 GGCCC G^G[ACGT]CC
615 GGGCC
SfaNI 226 GATGC GCATC(5/9)
474 GATGC
StyI 245 CCATGG C^C[AT][AT]GG
538 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EaeI EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIV Sau3AI ScrFI
TseI TspRI