(Circular) (Six-base) MAPSORT of: pFN21AB8894.seq Check: 6425 from: 1 to: 5356 pFN21AB8894 5356 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3166 278 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2699 1937 Size: 762 4594 Acc65I G'GTAC_C Cuts at: 2677 2677 Size: 5356 AclI AA'CG_TT Cuts at: 1563 3483 3856 1563 Size: 1920 373 3063 Fragments arranged by size: 3063 1920 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3419 4803 5071 1632 Size: 1787 1384 268 1917 Fragments arranged by size: 1917 1787 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5159 AflIII A'CryG_T Cuts at: 1203 4255 5232 1203 Size: 3052 977 1327 Fragments arranged by size: 3052 1327 977 AgeI A'CCGG_T Cuts at: 4187 4187 Size: 5356 AhdI GACnn_n'nnGTC Cuts at: 4085 4085 Size: 5356 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5356 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5356 AlwNI CAG_nnn'CTG Cuts at: 2106 3099 4671 2106 Size: 993 1572 2791 Fragments arranged by size: 2791 1572 993 ApaI G_GGCC'C Cuts at: 2271 2641 2271 Size: 370 4986 ApaLI G'TGCA_C Cuts at: 2042 3413 4569 2042 Size: 1371 1156 2829 Fragments arranged by size: 2829 1371 1156 ApoI r'AATT_y Cuts at: 1493 2665 2891 3043 5152 1493 Size: 1172 226 152 2109 1697 Fragments arranged by size: 2109 1697 1172 226 152 AseI AT'TA_AT Cuts at: 160 1032 3910 160 Size: 872 2878 1606 Fragments arranged by size: 2878 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 AvaI C'yCGr_G Cuts at: 1870 1943 2156 2201 2272 2681 1870 Size: 73 213 45 71 409 4545 Fragments arranged by size: 4545 409 213 73 71 45 BamHI G'GATC_C Cuts at: 2686 3053 2686 Size: 367 4989 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2216 2251 2261 2309 2677 Size: 325 217 627 429 35 10 48 368 Cuts at: 2677 4132 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 429 368 325 217 48 35 10 BanII G_rGCy'C Cuts at: 1391 2271 2641 2649 2675 1391 Size: 880 370 8 26 4072 Fragments arranged by size: 4072 880 370 26 8 BbeI G_GCGC'C Cuts at: 2220 2265 2220 Size: 45 5311 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2528 928 Size: 971 629 3756 Fragments arranged by size: 3756 971 629 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3547 3581 1903 Size: 34 1610 34 3678 Fragments arranged by size: 3678 1610 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3248 4458 3248 Size: 1210 4146 BclI T'GATC_A Cuts at: 1548 1548 Size: 5356 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2507 2616 3967 Size: 107 122 71 631 605 835 109 1351 Cuts at: 3967 136 Size: 1525 Fragments arranged by size: 1525 1351 835 631 605 122 109 107 71 BglII A'GATC_T Cuts at: 5351 5351 Size: 5356 BlpI GC'TnA_GC Cuts at: 2773 2773 Size: 5356 Bme1580I G_kGCm'C Cuts at: 1790 2046 2271 2641 3417 4573 1790 Size: 256 225 370 776 1156 2573 Fragments arranged by size: 2573 1156 776 370 256 225 BmgBI CAC'GTC Cuts at: 1318 2530 2632 1318 Size: 1212 102 4042 Fragments arranged by size: 4042 1212 102 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3087 4045 442 Size: 416 53 83 399 24 1670 958 1753 Fragments arranged by size: 1753 1670 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2663 4016 1239 Size: 1424 1353 2579 Fragments arranged by size: 2579 1424 1353 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2633 3481 4346 4644 2633 Size: 848 865 298 3345 Fragments arranged by size: 3345 865 848 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4019 882 Size: 617 2520 2219 Fragments arranged by size: 2520 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3147 493 Size: 711 1943 2702 Fragments arranged by size: 2702 1943 711 BsaBI GATnn'nnATC Cuts at: 5350 5350 Size: 5356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2217 2262 3163 3545 Size: 53 83 186 936 684 45 901 382 Cuts at: 3545 4180 275 Size: 635 1451 Fragments arranged by size: 1451 936 901 684 635 382 186 83 53 45 BsaWI w'CCGG_w Cuts at: 3789 4187 4461 4608 5019 3789 Size: 398 274 147 411 4126 Fragments arranged by size: 4126 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5131 Fragments arranged by size: 5131 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2552 1186 Size: 946 420 3990 Fragments arranged by size: 3990 946 420 BseYI C'CCAG_C Cuts at: 4559 5055 4559 Size: 496 4860 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4783 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2806 3567 4595 1540 Size: 275 186 805 761 1028 2301 Fragments arranged by size: 2301 1028 805 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 2649 3417 3502 4573 2046 Size: 603 768 85 1071 2829 Fragments arranged by size: 2829 1071 768 603 85 BsmI GAATG_Cn' Cuts at: 2867 2960 2867 Size: 93 5263 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2271 2641 2649 2675 3417 3502 Size: 399 256 225 370 8 26 742 85 Cuts at: 3502 4573 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 370 256 225 85 26 8 BspEI T'CCGG_A Cuts at: 5019 5019 Size: 5356 BspHI T'CATG_A Cuts at: 3245 4975 3245 Size: 1730 3626 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2472 2644 2711 844 Size: 1628 172 67 3489 Fragments arranged by size: 3489 1628 172 67 BsrBI CCG'CTC Cuts at: 1394 1823 2329 3243 1394 Size: 429 506 914 3507 Fragments arranged by size: 3507 914 506 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3851 4025 65 Size: 1183 2603 174 1396 Fragments arranged by size: 2603 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2222 4000 4187 1700 Size: 110 412 1778 187 2869 Fragments arranged by size: 2869 1778 412 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5356 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5356 BssSI C'ACGA_G Cuts at: 2048 3416 4428 2048 Size: 1368 1012 2976 Fragments arranged by size: 2976 1368 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5356 BstBI TT'CG_AA Cuts at: 4160 4160 Size: 5356 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5356 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5356 BstYI r'GATC_y Cuts at: 1972 2686 3053 3438 3455 4907 4993 5005 5351 Size: 714 367 385 17 1452 86 12 346 Cuts at: 5351 1972 Size: 1977 Fragments arranged by size: 1977 1452 714 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2134 513 Size: 552 1069 3735 Fragments arranged by size: 3735 1069 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2431 487 Size: 683 56 1205 3412 Fragments arranged by size: 3412 1205 683 56 BtsI GCAGTG_nn' Cuts at: 2872 3666 3686 2872 Size: 794 20 4542 Fragments arranged by size: 4542 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5356 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5321 DraI TTT'AAA Cuts at: 2660 2660 Size: 5356 DraIII CAC_nnn'GTG Cuts at: 2038 2038 Size: 5356 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4363 809 Size: 507 3047 1802 Fragments arranged by size: 3047 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2149 2433 2469 Size: 54 1425 50 275 18 319 284 36 Cuts at: 2469 2621 2803 3692 5321 8 Size: 152 182 889 1629 43 Fragments arranged by size: 1629 1425 889 319 284 275 182 152 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 2803 1537 Size: 275 991 4090 Fragments arranged by size: 4090 991 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2537 3286 1353 Size: 1184 749 3423 Fragments arranged by size: 3423 1184 749 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2510 3943 4317 4463 387 Size: 1064 138 921 1433 374 146 1280 Fragments arranged by size: 1433 1280 1064 921 374 146 138 EcoICRI GAG'CTC Cuts at: 2647 2647 Size: 5356 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2663 3419 4016 4803 5071 1239 Size: 393 1031 756 597 787 268 1524 Fragments arranged by size: 1524 1031 787 756 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2268 2638 2268 Size: 370 4986 EcoRI G'AATT_C Cuts at: 2665 3043 2665 Size: 378 4978 FspI TGC'GCA Cuts at: 3862 3862 Size: 5356 HaeII r_GCGC'y Cuts at: 1678 2220 2265 2305 2358 4503 1678 Size: 542 45 40 53 2145 2531 Fragments arranged by size: 2531 2145 542 53 45 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3997 4029 4071 4103 1373 Size: 32 135 32 2425 32 42 32 2626 Fragments arranged by size: 2626 2425 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2700 2946 669 Size: 988 281 762 246 3079 Fragments arranged by size: 3079 988 762 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5356 HpaI GTT'AAC Cuts at: 2946 2946 Size: 5356 KasI G'GCGC_C Cuts at: 2216 2261 2216 Size: 45 5311 KpnI G_GTAC'C Cuts at: 2681 2681 Size: 5356 MfeI C'AATT_G Cuts at: 2955 2955 Size: 5356 MluI A'CGCG_T Cuts at: 5232 5232 Size: 5356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4470 4654 1305 Size: 3165 184 2007 Fragments arranged by size: 3165 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2435 2471 10 Size: 54 1425 343 603 36 2895 Fragments arranged by size: 2895 1425 603 343 54 36 MslI CAynn'nnrTG Cuts at: 518 1166 3314 3673 3832 518 Size: 648 2148 359 159 2042 Fragments arranged by size: 2148 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2084 2106 2214 2772 3449 4597 4842 1606 Size: 478 22 108 558 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 558 478 245 108 22 NaeI GCC'GGC Cuts at: 2224 2224 Size: 5356 NarI GG'CG_CC Cuts at: 2217 2262 2217 Size: 45 5311 NcoI C'CATG_G Cuts at: 513 1065 2134 513 Size: 552 1069 3735 Fragments arranged by size: 3735 1069 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5356 NgoMIV G'CCGG_C Cuts at: 2222 2222 Size: 5356 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5356 NotI GC'GGCC_GC Cuts at: 2803 2803 Size: 5356 NspI r_CATG'y Cuts at: 1132 1229 2714 4259 1132 Size: 97 1485 1545 2229 Fragments arranged by size: 2229 1545 1485 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5356 PciI A'CATG_T Cuts at: 4255 4255 Size: 5356 PflMI CCAn_nnn'nTGG Cuts at: 1108 2038 1108 Size: 930 4426 PmeI GTTT'AAAC Cuts at: 2660 2660 Size: 5356 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3425 3457 4938 4970 3425 Size: 32 1481 32 3811 Fragments arranged by size: 3811 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5356 PsiI TTA'TAA Cuts at: 2926 2926 Size: 5356 PspOMI G'GGCC_C Cuts at: 2267 2637 2267 Size: 370 4986 PstI C_TGCA'G Cuts at: 830 2111 2708 830 Size: 1281 597 3478 Fragments arranged by size: 3478 1281 597 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5356 PvuII CAG'CTG Cuts at: 2084 2084 Size: 5356 SacI G_AGCT'C Cuts at: 2649 2649 Size: 5356 SalI G'TCGA_C Cuts at: 1936 2698 1936 Size: 762 4594 SbfI CC_TGCA'GG Cuts at: 2708 2708 Size: 5356 ScaI AGT'ACT Cuts at: 3604 5316 3604 Size: 1712 3644 SfcI C'TryA_G Cuts at: 826 1045 2107 2176 2194 2704 3839 4520 4711 Size: 219 1062 69 18 510 1135 681 191 Cuts at: 4711 826 Size: 1471 Fragments arranged by size: 1471 1135 1062 681 510 219 191 69 18 SfoI GGC'GCC Cuts at: 2218 2263 2218 Size: 45 5311 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5356 SmaI CCC'GGG Cuts at: 1872 2274 2683 1872 Size: 402 409 4545 Fragments arranged by size: 4545 409 402 SmlI C'TyrA_G Cuts at: 820 1017 1943 2648 3460 4361 4623 820 Size: 197 926 705 812 901 262 1553 Fragments arranged by size: 1553 926 901 812 705 262 197 SnaBI TAC'GTA Cuts at: 493 3147 493 Size: 2654 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5356 SphI G_CATG'C Cuts at: 1132 2714 1132 Size: 1582 3774 SspI AAT'ATT Cuts at: 5 52 3280 5 Size: 47 3228 2081 Fragments arranged by size: 3228 2081 47 StuI AGG'CCT Cuts at: 1517 2501 1517 Size: 984 4372 StyI C'CwwG_G Cuts at: 513 1065 2134 2472 2795 513 Size: 552 1069 338 323 3074 Fragments arranged by size: 3074 1069 552 338 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2540 3383 3400 3553 3738 4183 325 Size: 1255 960 843 17 153 185 445 1498 Fragments arranged by size: 1498 1255 960 843 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3602 5314 96 Size: 274 80 33 51 1443 1625 1712 138 Fragments arranged by size: 1712 1625 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5316 5348 5316 Size: 32 5324 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5356 XbaI T'CTAG_A Cuts at: 2692 2692 Size: 5356 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5356 XmaI C'CCGG_G Cuts at: 1870 2272 2681 1870 Size: 402 409 4545 Fragments arranged by size: 4545 409 402 XmnI GAAnn'nnTTC Cuts at: 3485 3485 Size: 5356 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3164 276 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI