Restriction map for pFN21AB8876
 (Circular) (Six-base) MAPSORT of: pFN21AB8876.seq  Check: 7875  from: 1  to: 5206



pFN21AB8876  5206 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:27  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3016     278 
   Size:         53      83     186     936    1480    2468

  Fragments arranged by size: 

                2468    1480     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2247    2549    1937 
   Size:        310     302    4594

  Fragments arranged by size: 

                4594     310     302
 
Acc65I G'GTAC_C

Cuts at:   2527    2527 
   Size:       5206
 
AclI AA'CG_TT

Cuts at:   1563    3333    3706    1563 
   Size:       1770     373    3063

  Fragments arranged by size: 

                3063    1770     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2400    3269    4653    4921    1632 
   Size:        768     869    1384     268    1917

  Fragments arranged by size: 

                1917    1384     869     768     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5009
 
AflIII A'CryG_T

Cuts at:   1203    4105    5082    1203 
   Size:       2902     977    1327

  Fragments arranged by size: 

                2902    1327     977
 
AgeI A'CCGG_T

Cuts at:   2177    4037    2177 
   Size:       1860    3346
 
AhdI GACnn_n'nnGTC

Cuts at:   3935    3935 
   Size:       5206
 
AleI CACnn'nnGTG

Cuts at:   1166    2432    1166 
   Size:       1266    3940
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5206
 
AlwNI CAG_nnn'CTG

Cuts at:   2239    2949    4521    2239 
   Size:        710    1572    2924

  Fragments arranged by size: 

                2924    1572     710
 
ApaLI G'TGCA_C

Cuts at:   3263    4419    3263 
   Size:       1156    4050
 
ApoI r'AATT_y

Cuts at:   1493    2515    2741    2893    5002    1493 
   Size:       1022     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1022     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3760     160 
   Size:        872    2728    1606

  Fragments arranged by size: 

                2728    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5206
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2531    1870 
   Size:         73     588    4545

  Fragments arranged by size: 

                4545     588      73
 
BamHI G'GATC_C

Cuts at:   2536    2903    2536 
   Size:        367    4839
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2527    3982     618 
   Size:        325     217     627     740    1455    1842

  Fragments arranged by size: 

                1842    1455     740     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2202    2525    1391 
   Size:        811     323    4072

  Fragments arranged by size: 

                4072     811     323
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4235
 
BbvCI CC'TCA_GC

Cuts at:   2492    2492 
   Size:       5206
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3397    3431    1903 
   Size:         34      50      34    1376      34    3678

  Fragments arranged by size: 

                3678    1376      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3098    4308    3098 
   Size:       1210    3996
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5206
 
BfrBI ATG'CAT

Cuts at:   2008    2008 
   Size:       5206
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3817     136 
   Size:        107     122      71     631     605    2145    1525

  Fragments arranged by size: 

                2145    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5201    5201 
   Size:       5206
 
BlpI GC'TnA_GC

Cuts at:   2623    2623 
   Size:       5206
 
Bme1580I G_kGCm'C

Cuts at:   1790    2073    3267    4423    1790 
   Size:        283    1194    1156    2573

  Fragments arranged by size: 

                2573    1194    1156     283
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5206
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2937    3895     442 
   Size:        416      53      83     399      24    1520     958    1753

  Fragments arranged by size: 

                1753    1520     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5206
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2327    2399    3866    1239 
   Size:       1088      72    1467    2579

  Fragments arranged by size: 

                2579    1467    1088      72
 
Bpu10I CC'TnA_GC

Cuts at:   2444    2492    2444 
   Size:         48    5158
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3331    4196    4494    3331 
   Size:        865     298    4043

  Fragments arranged by size: 

                4043     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2375    2466    3869     882 
   Size:        617     876      91    1403    2219

  Fragments arranged by size: 

                2219    1403     876     617      91
 
BsaAI yAC'GTr

Cuts at:    493    1204    2332    2997     493 
   Size:        711    1128     665    2702

  Fragments arranged by size: 

                2702    1128     711     665
 
BsaBI GATnn'nnATC

Cuts at:   5200    5200 
   Size:       5206
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3013    3395    4030     275 
   Size:         53      83     186     936    1480     382     635    1451

  Fragments arranged by size: 

                1480    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2177    3639    4037    4311    4458    4869    2177 
   Size:       1462     398     274     147     411    2514

  Fragments arranged by size: 

                2514    1462     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4981

  Fragments arranged by size: 

                4981     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2149    1186 
   Size:        963    4243
 
BseYI C'CCAG_C

Cuts at:   4409    4905    4409 
   Size:        496    4710
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4633
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2656    3417    4445    1540 
   Size:        275     186     655     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     655     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3267    3352    4423    3267 
   Size:         85    1071    4050

  Fragments arranged by size: 

                4050    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2717    2810    2717 
   Size:         93    5113
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2073    2202    2525    3267    3352    4423    1391 
   Size:        399     283     129     323     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     323     283     129      85
            
 
BspEI T'CCGG_A

Cuts at:   4869    4869 
   Size:       5206
 
BspHI T'CATG_A

Cuts at:   3095    4825    3095 
   Size:       1730    3476
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2561     844 
   Size:       1717    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3093    1394 
   Size:        429    1270    3507

  Fragments arranged by size: 

                3507    1270     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3701    3875      65 
   Size:       1183    2453     174    1396

  Fragments arranged by size: 

                2453    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2177    3850    4037    1700 
   Size:        110     367    1673     187    2869

  Fragments arranged by size: 

                2869    1673     367     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5206
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5206
 
BssSI C'ACGA_G

Cuts at:   3266    4278    3266 
   Size:       1012    4194
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2473     794 
   Size:       1679    3527
 
BstBI TT'CG_AA

Cuts at:   4010    4010 
   Size:       5206
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5206
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2305    2377    2434    2305 
   Size:         72      57    5077

  Fragments arranged by size: 

                5077      72      57
 
BstYI r'GATC_y

Cuts at:   1972    2256    2320    2536    2903    3288    3305    4757    4843 
   Size:        284      64     216     367     385      17    1452      86

Cuts at:   4843    4855    5201    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     385     367     346     284     216      86
                  64      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4654
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4467

  Fragments arranged by size: 

                4467     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2722    3516    3536    2722 
   Size:        794      20    4392

  Fragments arranged by size: 

                4392     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5206
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5171
 
DraI TTT'AAA

Cuts at:   2510    2510 
   Size:       5206
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4213     809 
   Size:        507    2897    1802

  Fragments arranged by size: 

                2897    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2653    3542    5171 
   Size:         54    1425      50     275      18     823     889    1629

Cuts at:   5171       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     823     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2653    1537 
   Size:        275     841    4090

  Fragments arranged by size: 

                4090     841     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3136    1353 
   Size:       1783    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3793    4167    4313     387 
   Size:       1064     138    2204     374     146    1280

  Fragments arranged by size: 

                2204    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2327    2399    2400    3269    3866    4653    4921 
   Size:        393     695      72       1     869     597     787     268

Cuts at:   4921    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     869     787     695     597     393     268      72
                   1
 
EcoRI G'AATT_C

Cuts at:   2515    2893    2515 
   Size:        378    4828
 
FspI TGC'GCA

Cuts at:   3712    3712 
   Size:       5206
 
HaeII r_GCGC'y

Cuts at:   1678    4353    1678 
   Size:       2675    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3847    3879    3921    3953    1373 
   Size:         32     135      32    2275      32      42      32    2626

  Fragments arranged by size: 

                2626    2275     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2550    2796     669 
   Size:        988     281     612     246    3079

  Fragments arranged by size: 

                3079     988     612     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2412     748 
   Size:       1664    3542
 
HpaI GTT'AAC

Cuts at:   2796    2796 
   Size:       5206
 
KpnI G_GTAC'C

Cuts at:   2531    2531 
   Size:       5206
 
MfeI C'AATT_G

Cuts at:   2115    2805    2115 
   Size:        690    4516
 
MluI A'CGCG_T

Cuts at:   5082    5082 
   Size:       5206
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2322    4320    4504    1305 
   Size:       1017    1998     184    2007

  Fragments arranged by size: 

                2007    1998    1017     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3384

  Fragments arranged by size: 

                3384    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2267    2432    3164    3523    3682     518 
   Size:        648    1101     165     732     359     159    2042

  Fragments arranged by size: 

                2042    1101     732     648     359     165     159
 
MspA1I CmG'CkG

Cuts at:   1606    2485    2622    3299    4447    4692    1606 
   Size:        879     137     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     879     677     245     137
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4654
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5206
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5206
 
NotI GC'GGCC_GC

Cuts at:   2653    2653 
   Size:       5206
 
NsiI A_TGCA'T

Cuts at:   2010    2010 
   Size:       5206
 
NspI r_CATG'y

Cuts at:   1132    1229    2476    2564    4109    1132 
   Size:         97    1247      88    1545    2229

  Fragments arranged by size: 

                2229    1545    1247      97      88
 
PasI CC'CwG_GG

Cuts at:   1348    2364    2498    1348 
   Size:       1016     134    4056

  Fragments arranged by size: 

                4056    1016     134
 
PciI A'CATG_T

Cuts at:   4105    4105 
   Size:       5206
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2010    2239    1108 
   Size:        902     229    4075

  Fragments arranged by size: 

                4075     902     229
 
PfoI T'CCnGG_A

Cuts at:   2316    2406    2316 
   Size:         90    5116
 
PmeI GTTT'AAAC

Cuts at:   2510    2510 
   Size:       5206
 
PmlI CAC'GTG

Cuts at:   2332    2332 
   Size:       5206
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3275    3307    4788    4820    3275 
   Size:         32    1481      32    3661

  Fragments arranged by size: 

                3661    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    2117    1613 
   Size:        504    4702
 
PsiI TTA'TAA

Cuts at:   2776    2776 
   Size:       5206
 
PstI C_TGCA'G

Cuts at:    830    2457    2469    2558     830 
   Size:       1627      12      89    3478

  Fragments arranged by size: 

                3478    1627      89      12
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5206
 
PvuII CAG'CTG

Cuts at:   2485    2485 
   Size:       5206
 
SalI G'TCGA_C

Cuts at:   1936    2548    1936 
   Size:        612    4594
 
SbfI CC_TGCA'GG

Cuts at:   2558    2558 
   Size:       5206
 
ScaI AGT'ACT

Cuts at:   3454    5166    3454 
   Size:       1712    3494
 
SfcI C'TryA_G

Cuts at:    826    1045    2453    2465    2554    3689    4370    4561     826 
   Size:        219    1408      12      89    1135     681     191    1471

  Fragments arranged by size: 

                1471    1408    1135     681     219     191      89      12
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5206
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5206
 
SmaI CCC'GGG

Cuts at:   1872    2533    1872 
   Size:        661    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3310    4211    4473     820 
   Size:        197     926    1367     901     262    1553

  Fragments arranged by size: 

                1553    1367     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2997     493 
   Size:       2504    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5206
 
SphI G_CATG'C

Cuts at:   1132    2564    1132 
   Size:       1432    3774
 
SspI AAT'ATT

Cuts at:      5      52    3130       5 
   Size:         47    3078    2081

  Fragments arranged by size: 

                3078    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5206
 
StyI C'CwwG_G

Cuts at:    513    1065    2385    2645     513 
   Size:        552    1320     260    3074

  Fragments arranged by size: 

                3074    1320     552     260
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2490    3233    3250    3403    3588    4033     325 
   Size:       1255     910     743      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255     910     743     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3452    5164      96 
   Size:        274      80      33      51    1443    1475    1712     138

  Fragments arranged by size: 

                1712    1475    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2462    2494    5166    5198    2462 
   Size:         32    2672      32    2470

  Fragments arranged by size: 

                2672    2470      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5206
 
XbaI T'CTAG_A

Cuts at:   2542    2542 
   Size:       5206
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2434    2434 
   Size:       5206
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5206
 
XmaI C'CCGG_G

Cuts at:   1870    2531    1870 
   Size:        661    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3335    3335 
   Size:       5206
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3014     276 
   Size:         53      83     186     936    1480    2468

  Fragments arranged by size: 

                2468    1480     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
     PstI     PvuI    PvuII     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
     BplI    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     PacI    PpuMI   PspOMI    PspXI     PsrI    RsrII
     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI
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