Restriction map for
pFN21AB8876
(Linear) (Six-base) MAPSORT of: KIBB8876.seq Check: 246 from: 1 to: 501
KIBB8876.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AccI GT'mk_AC
Cuts at: 0 242 501
Size: 242 259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 501
Size: 395 106
AgeI A'CCGG_T
Cuts at: 0 172 501
Size: 172 329
AleI CACnn'nnGTG
Cuts at: 0 427 501
Size: 427 74
AlwNI CAG_nnn'CTG
Cuts at: 0 234 501
Size: 234 267
BanII G_rGCy'C
Cuts at: 0 197 501
Size: 197 304
BbvCI CC'TCA_GC
Cuts at: 0 487 501
Size: 487 14
BfrBI ATG'CAT
Cuts at: 0 3 501
Size: 3 498
Bme1580I G_kGCm'C
Cuts at: 0 68 501
Size: 68 433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 394 501
Size: 322 72 107
Fragments arranged by size:
322 107 72
Bpu10I CC'TnA_GC
Cuts at: 0 439 487 501
Size: 439 48 14
Fragments arranged by size:
439 48 14
BsaI GGTCTCn'nnnn_
Cuts at: 0 370 461 501
Size: 370 91 40
Fragments arranged by size:
370 91 40
BsaAI yAC'GTr
Cuts at: 0 327 501
Size: 327 174
BsaWI w'CCGG_w
Cuts at: 0 172 501
Size: 172 329
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 144 501
Size: 144 357
Bsp1286I G_dGCh'C
Cuts at: 0 68 197 501
Size: 68 129 304
Fragments arranged by size:
304 129 68
BsrFI r'CCGG_y
Cuts at: 0 172 501
Size: 172 329
BstAPI GCAn_nnn'nTGC
Cuts at: 0 468 501
Size: 468 33
BstXI CCAn_nnnn'nTGG
Cuts at: 0 300 372 429 501
Size: 300 72 57 72
Fragments arranged by size:
300 72 72 57
BstYI r'GATC_y
Cuts at: 0 251 315 501
Size: 251 64 186
Fragments arranged by size:
251 186 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 394 395 501
Size: 322 72 1 106
Fragments arranged by size:
322 106 72 1
HindIII A'AGCT_T
Cuts at: 0 407 501
Size: 407 94
MfeI C'AATT_G
Cuts at: 0 110 501
Size: 110 391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 501
Size: 317 184
MslI CAynn'nnrTG
Cuts at: 0 262 427 501
Size: 262 165 74
Fragments arranged by size:
262 165 74
MspA1I CmG'CkG
Cuts at: 0 480 501
Size: 480 21
NsiI A_TGCA'T
Cuts at: 0 5 501
Size: 5 496
NspI r_CATG'y
Cuts at: 0 471 501
Size: 471 30
PasI CC'CwG_GG
Cuts at: 0 359 493 501
Size: 359 134 8
Fragments arranged by size:
359 134 8
PflMI CCAn_nnn'nTGG
Cuts at: 0 234 501
Size: 234 267
PfoI T'CCnGG_A
Cuts at: 0 311 401 501
Size: 311 90 100
Fragments arranged by size:
311 100 90
PmlI CAC'GTG
Cuts at: 0 327 501
Size: 327 174
PshAI GACnn'nnGTC
Cuts at: 0 112 501
Size: 112 389
PstI C_TGCA'G
Cuts at: 0 452 464 501
Size: 452 12 37
Fragments arranged by size:
452 37 12
PvuII CAG'CTG
Cuts at: 0 480 501
Size: 480 21
SfcI C'TryA_G
Cuts at: 0 448 460 501
Size: 448 12 41
Fragments arranged by size:
448 41 12
StyI C'CwwG_G
Cuts at: 0 380 501
Size: 380 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 485 501
Size: 485 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 457 489 501
Size: 457 32 12
Fragments arranged by size:
457 32 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 429 501
Size: 429 72
Enzymes that do cut:
AccI AcuI AgeI AleI AlwNI BanII BbvCI BfrBI
Bme1580I BpmI Bpu10I BsaI BsaAI BsaWI BseRI Bsp1286I
BsrFI BstAPI BstXI BstYI Eco57MI HindIII MfeI MmeI
MslI MspA1I NsiI NspI PasI PflMI PfoI PmlI
PshAI PstI PvuII SfcI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpuEI BsaBI BsaHI BsaXI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HpaI KasI KpnI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PciI PmeI
PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI